LOCUS       sequence-475542-        8565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NUP98::SETBP1 in bicistronic retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8565)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 8565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CL20_MSCV-NUP98::SETBP1-ires-GFP
FEATURES             Location/Qualifiers
     source          1..8565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..27
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(246..350)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     418..436
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(474..496)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     596..615
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     809..831
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     959..978
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             1178..1694
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1758..2099
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2166..2582
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2512..2534
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2550..2566
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    2609..2633
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4698..4722)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4735..5302
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4752..5304
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4896..4913)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5123..5142
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5316..5318,5319..5321,5322..6035)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5361..5382)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5622..5641)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5969..5990
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6137..6651
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6813..6829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6837..6843,6844..6861,6862..6867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6882..6903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7020..7037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7191..7779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7271..7290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7950..8565,1..176,177..245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
       61 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
      121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
      181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
      241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
      361 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
      421 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
      481 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
      541 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
      601 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
      661 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
      721 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      781 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa
      841 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
      901 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
      961 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     1021 tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg
     1081 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     1141 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     1201 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     1261 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     1321 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     1381 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     1441 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     1501 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     1561 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
     1621 cgaatcgtgg actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc
     1681 gggggtcttt catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga
     1741 cccccccgcc gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc
     1801 gtgtttgtgc cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct
     1861 gtatctggcg gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga
     1921 gacgtcccag ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt
     1981 ggaatccgac cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc
     2041 cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc
     2101 agcgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa
     2161 attagggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg
     2221 agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct
     2281 ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc
     2341 tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg
     2401 tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct
     2461 ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac
     2521 ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg
     2581 ccggaattag atctctcgag gttatcaaac aagtttgtac aaaaaagcag gctccgcggc
     2641 cgcccccttc accatgttta acaaatcatt tggaacaccc tttgggggtg gcacaggtgg
     2701 ctttggcaca acttcaacat ttggacagaa tactggcttt ggcactacta gtggaggggc
     2761 atttggaaca tctgcatttg gttctagcaa caatactgga ggcctctttg gaaattcaca
     2821 gactaaacca ggaggattgt ttggaaccag ttcatttagc cagccagcta cctccacaag
     2881 cactggcttt gggtttggta cgtcaacagg aacagcaaat accttgtttg gaactgcaag
     2941 cacagggacc agtctcttct catcccaaaa caatgccttt gcacaaaata aaccaactgg
     3001 ctttggcaat tttggaacca gtactagcag tggaggactc tttggaacca caaataccac
     3061 ctctaatcct tttggcagca catctggctc cctctttggg ccaagtagtt ttacagctgc
     3121 tcctactggg actactatta aatttaaccc tccaactggt acagatacta tggtcaaagc
     3181 tggagttagc actaacataa gtaccaagca ccagtgtatt actgctatga aagaatatga
     3241 aagcaagtca ctagaggaac ttcgtttaga ggattatcag gctaacagga agggcccaca
     3301 gaaccaggtg ggagcaggta ccacaactgg cttgtttggg tcttctccag ccacttccag
     3361 cgcaacagga ctcttcagct cctccaccac taattcaggc tttgcatatg gtcagaacaa
     3421 aactgccttt ggaactagta caactggatt tggaacaaat ccaggtggtc tctttggcca
     3481 acagaatcag cagactacca gcctcttcag caaaccattt ggccaggcta caaccaccca
     3541 gaacactggc ttttcctttg gtaataccag caccatagga cagccaagca ccaacaccat
     3601 gggattattt ggagtaaccc aagcctcaca gcctggaggt ctttttggga cagctacaaa
     3661 caccagcact gggacagcat ttggaacagg aacaggtctc tttgggcaga ccaatactgg
     3721 atttggtgct gttggttcga ccctgtttgg caataacaag cttactacat ttggaagcag
     3781 cacaaccagt gcaccttcat ttggtacaac cagtggcggg ctctttggtt ttggcacaaa
     3841 taccagtggg aatagtattt ttggaagtaa accagcacct gggactcttg gaactgggct
     3901 tggtgcagga tttggaacag ctcttggtgc tggacaggca tctttgtttg ggaacaacca
     3961 acctaagatt ggagggcctc ttggtacagg agcctttggg gcccctggat ttaatactac
     4021 gacagccact ttgggctttg gagcccccca ggccccagta gtcggctcct ccctgaagaa
     4081 gaggttcaag cggcgggaga tcgaagccat ccagtgcgaa gtgcggaaga tgtgcaacta
     4141 caccaagatc ctgtccacca agaagaacct ggaccacgtg aacaagatcc tgaaggccaa
     4201 gcggctgcag agacaatcaa aaacaggcaa caacttcgtg aagaagaggc gcgggcgtcc
     4261 caggaagcag cccacccagt tcgatgagga ctccagagac caaatgccgg tgctggaaaa
     4321 atgcatcgac ctgcccagca aaagaggcca gaagcccagc ctgagcccgc tggtgctgga
     4381 gcccgccgcc agccaagaca ccatcatggc caccatcgag gcggtcatcc acatggcccg
     4441 ggaggcgccg cccctgcccc cgccaccgcc gccgcccctg ccgccaccgc cgccaccacc
     4501 cctgcccccg ccaccccctc tacccaagac cccccgaggc ggaaagagga aacacaaacc
     4561 gcaggccccc gctcagcccc cacagcagtc gcccccgcag cagccccttc cccaggaaga
     4621 ggaggtgaaa gccaaaaggc agaggaagtc ccgagggagt gagagcgagg tccttcccta
     4681 gaagggtggg cgcgccgacc cagctttctt gtacaaagtg gttcgataac gaattccgcc
     4741 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     4801 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     4861 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     4921 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     4981 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5041 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5101 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5161 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5221 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     5281 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     5341 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     5401 gcgtgtctgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     5461 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     5521 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     5581 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     5641 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5701 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5761 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc
     5821 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5881 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5941 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     6001 ggatcactct cggcatggac gagctgtaca agtaatgaat taattaagaa ttatcaagct
     6061 tatcgatacc gtcgacctgc agccaagctt atcgataaaa taaaagattt tatttagtct
     6121 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     6181 gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta
     6241 ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     6301 ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag
     6361 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     6421 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     6481 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     6541 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     6601 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     6661 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     6721 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     6781 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     6841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtca
//
