LOCUS       sequence-475543-       13320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhOMT1 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS-GhOMT1_1
FEATURES             Location/Qualifiers
     source          1..13320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6406..6422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6415..6437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6625..6649
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7950..8579
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9008..10081
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10147..10341
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10577..10599)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10685..10825
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10740..10759
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10840..10857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11011..11599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11091..11110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11686..12480)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12905..12929
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13197..13218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13233..13238,13239..13256,13257..13263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13271..13287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13276..13298
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13295..13311
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13295..13311
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagttc ctggatattc
     3781 aaatgctgtc atcccgtatg ccttactaaa aggaacccct ccttctagta tagcatcatt
     3841 taaatggtac cagctttgca tgaagacttg gtcatggtgc aacaaaagga gaggtgcaat
     3901 cgaccctcca tcttgattct taacaaggaa cttgcatagt gggcctgcac cgtacagtct
     3961 ttcaatttct tctttttcct ttgcttttac tgcgcatttg agaatggaat ggctggccaa
     4021 caggcgtagc attcgatcta gtagcactgg tgcgtcagga ttctttgaag gaagggcact
     4081 cgcaatctgg gaaggtgaca gaaacgcgcc gtcaccagcg gctaagattg tgtcaattat
     4141 gttcagctcc aaggctgatt tcaagaccat tggaagtacc acggcgttgg ctaggcggac
     4201 ggccagtttc ccaacttctt cctcttgatc ttcttgagac atggtttctt tctcagcgcc
     4261 tgtttctgac tagtgaattc gctagcgtta actggccata atcctgtgta tgcaacgttg
     4321 aaaattcgga tctattttta cgattcgatc atgaattaat aaaatttaaa ttttattgaa
     4381 tgattttcta gtacataact gacatacgac ttatcagttg cgaaacgaac agctctaatt
     4441 acattgttaa gtgaaattac gcctaattcg tctaagtaca acataaccaa ctgtttaaac
     4501 ctaattaaat aagtcgaccc agaagctgtc agagatgtcg tccagacttg gaagttcagg
     4561 taagctttgt ggagatgcaa cgctctccgt aagttgtggt tgaatcggat ctggacgtgg
     4621 tatatacgcg tcctggtgta tagcagatcc tctactccgt atatcttgaa atatagggga
     4681 tttattatct cccagatata cacgccattc tctgcctgac gtgcagtgat gatttcccct
     4741 gtgcgtgaat ccatgaccgg tgcagcctat gtggaagtat atggtgcacc cacagtttag
     4801 atcaatgcgt cgtcgtctaa tcgccctcct cttggcttgc ctgtgtgcct tcttgataga
     4861 ggggggcgtc gagagtgatg aagataacat tcttgagagt ccaatgtttg agcgacgcat
     4921 tttccgcttt atccaggaaa tctttatagc tggatccctc gcctggattg caaagcacga
     4981 ttgaagggat ccctccttta atttgaactg gtttcccgta cttgcaattt gactgccagt
     5041 ctttttgggc cccaagaagc tctttccaat gctttagctt taggtattgc ggtgctacgt
     5101 catcaatgac gttataggca acattattcg agtacactcg attattgaag tccaggtgtc
     5161 cactgaggta attatgtggg cctaaagcac gagcccacat cgtcttgccc gttctagaat
     5221 caccttccac tatgagacta actggtctta caggccgcgc agcgggaccc attccaaaat
     5281 aatcatccgc ccattcttgc atctgttcag gaacgttagt gaaagaggaa agtggaaatg
     5341 gagggaccca ttgttcaggc gcctttttga aaaggcgttc caagttcgct gtaatgttat
     5401 ggtaactcac gatgaacgcc cttggatctc cggcccggat aattgcaaga gcttccgcca
     5461 aactaggtgc attgatggcg ttataataga cgtcgtcttt attcttcttt gtacccccag
     5521 gcactttgaa ctgtccggat tcacaacaat cgccgtcttt ggtgatgtaa ttcttgacgg
     5581 cgttggcgtc tttggctgct tgtatgtttg ggtgaaaatt ggtcgacctt ctggggtgag
     5641 taaggtcgaa aaatctctta ttcttgatgt tggactttcc agagagttgg atgagacagt
     5701 gaaggtgtgg gaatccgtcc gagtgctcct ctctggcaac tcgaatatat gttggtttga
     5761 cgactgacca tgataaggtt tgaagcattt gaagagcttc attttttggg atatcgcact
     5821 tgggataggt taagaaaata tgtctggctt gtaaacgaaa agaatcgggg tttctaggca
     5881 ttttttaaat agtgacccag gactcaaaaa ctagctctca acttctctca tatgttgtgg
     5941 agtctggagt cctttatata tgtttacgct atagaaggca tttggggact ctcccgggcc
     6001 caccatagga ctccataccg cggccatcta tataatatta ctgatggccg cgcggatttt
     6061 ggagccccct tttgtcgtta gcgcgacctt taattcaaat taaaggttgc ttttttctat
     6121 cgtccaatca ttttgcgcct gactagccta gatattagca acaacttcgc gactaagttg
     6181 ttgaccgtca agtataaatt aatgggctta tggcccaatg ggtatagagc aaaatgccca
     6241 agcgtgatgc cccgtggcgc ttattagcgg gtacgtctaa ggtaagtcga aatgccaact
     6301 actctccctt gagagtattg ggccctaaag ttaataaggg acctcgaggg ggggcccggt
     6361 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt
     6421 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6481 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6541 gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg
     6601 ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     6661 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     6721 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     6781 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     6841 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     6901 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     6961 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     7021 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     7081 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     7141 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     7201 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     7261 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     7321 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     7381 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     7441 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     7501 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     7561 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     7621 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     7681 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     7741 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     7801 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     7861 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     7921 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     7981 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     8041 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     8101 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     8161 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     8221 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     8281 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     8341 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     8401 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     8461 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
     8521 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
     8581 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
     8641 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
     8701 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
     8761 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
     8821 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
     8881 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
     8941 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
     9001 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
     9061 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
     9121 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
     9181 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
     9241 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
     9301 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
     9361 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
     9421 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
     9481 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
     9541 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
     9601 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
     9661 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
     9721 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
     9781 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
     9841 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
     9901 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
     9961 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
    10021 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
    10081 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtgt
    10141 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
    10201 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
    10261 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
    10321 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
    10381 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
    10441 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
    10501 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
    10561 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    10621 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    10681 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    10741 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    10801 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    11041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    11101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    11161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
    11461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11641 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
    11701 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    11761 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    11821 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    11881 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    11941 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    12001 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    12061 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    12121 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    12181 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    12241 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    12301 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    12361 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    12421 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    12481 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    12541 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    12601 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    12661 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    12721 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    12781 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    12841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    12901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    12961 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcggggatag ccctttggtc
    13021 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    13081 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    13141 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    13201 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    13261 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
//
