LOCUS       sequence-475545-       12284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA-tagged NUP98::JADE2 in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CCLMPC_HA-NUP98::JADE2
FEATURES             Location/Qualifiers
     source          1..12284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      916..1504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1405..1424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1658..1675
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1792..1813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1828..1833,1834..1851,1852..1858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1866..1882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1871..1893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1890..1906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1890..1906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1925..1945
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1927..1945
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2046..2425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2426..2629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2580..2600
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2644..2824
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2871..2996
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3489..3722
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3907..3951
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4248..4365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4968..4992
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5013..5039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(9364..9388)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        9417..9916
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9452..9475)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9849..9868
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      9937..9946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9943..10653
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10088..10106)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10356..10375
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             10775..11008
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11080..11201
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11117..11136)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11171..11190
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11241..11376
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11396..11415)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11397..11415)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11425..11442)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11425..11441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11434..11456)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11583..12038
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11670..11689)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11880..11901
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12064..12168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12169..12237,12238..12284,1..745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
       61 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
      901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
      961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1681 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     1801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1921 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt ggccattgca
     1981 tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc
     2041 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     2101 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2161 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2221 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2281 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2341 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2401 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2461 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2521 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2581 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     2641 ccggggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     2761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     2821 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     2881 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     2941 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     3001 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     3061 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     3121 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     3181 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     3241 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     3301 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     3361 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     3421 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     3481 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     3541 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     3601 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     3661 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     3721 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     3781 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     3841 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     3901 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     3961 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     4021 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     4081 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     4141 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     4201 cgacggtatc gataagctaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
     4261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     4321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     4381 gacagcagag atccagtttg ggaattagct tgatcgatta gtccaatttg ttaaagacag
     4441 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
     4501 gtacgagcca tagatagaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     4561 agaccccacc tgtaggtttg gcaagctagg atcaaggtta ggaacagaga gacagcagaa
     4621 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     4681 gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     4741 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac
     4801 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
     4861 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
     4921 agcccacaac ccctcactcg gcgcgatcta gatctcgaat cgaattcaca agtttgtaca
     4981 aaaaagcagg cttggaagga gttcgaacca tgtacccata cgatgttcca gattacgctt
     5041 ttaacaaatc atttggaaca ccctttgggg gtggcacagg tggctttggc acaacttcaa
     5101 catttggaca gaatactggc tttggcacta ctagtggagg ggcatttgga acatctgcat
     5161 ttggttctag caacaatact ggaggcctct ttggaaattc acagactaaa ccaggaggat
     5221 tgtttggaac cagttcattt agccagccag ctacctccac aagcactggc tttgggtttg
     5281 gtacgtcaac aggaacagca aataccttgt ttggaactgc aagcacaggg accagtctct
     5341 tctcatccca aaacaatgcc tttgcacaaa ataaaccaac tggctttggc aattttggaa
     5401 ccagtactag cagtggagga ctctttggaa ccacaaatac cacctctaat ccttttggca
     5461 gcacatctgg ctccctcttt gggccaagta gttttacagc tgctcctact gggactacta
     5521 ttaaatttaa ccctccaact ggtacagata ctatggtcaa agctggagtt agcactaaca
     5581 taagtaccaa gcaccagtgt attactgcta tgaaagaata tgaaagcaag tcactagagg
     5641 aacttcgttt agaggattat caggctaaca ggaagggccc acagaaccag gtgggagcag
     5701 gtaccacaac tggcttgttt gggtcttctc cagccacttc cagcgcaaca ggactcttca
     5761 gctcctccac cactaattca ggctttgcat atggtcagaa caaaactgcc tttggaacta
     5821 gtacaactgg atttggaaca aatccaggtg gtctctttgg ccaacagaat cagcagacta
     5881 ccagcctctt cagcaaacca tttggccagg ctacaaccac ccagaacact ggcttttcct
     5941 ttggtaatac cagcaccata ggacagccaa gcaccaacac catgggatta tttggagtaa
     6001 cccaagcctc acagcctgga ggtctttttg ggacagctac aaacaccagc actgggacag
     6061 catttggaac aggaacaggt ctctttgggc agaccaatac tggatttggt gctgttggtt
     6121 cgaccctgtt tggcaataac aagcttacta catttggaag cagcacaacc agtgcacctt
     6181 catttggtac aaccagtggc gggctctttg gttttggcac aaataccagt gggaatagta
     6241 tttttggaag taaaccagca cctgggactc ttggaactgg gcttggtgca ggatttggaa
     6301 cagctcttgg tgctggacag gcatctttgt ttgggaacaa ccaacctaag attggagggc
     6361 ctcttggtac aggagccttt ggggcccctg gatttaatac tacgacagcc actttgggct
     6421 ttggagcccc ccaggcccca gtagctttga cagatccaaa tgcttctgct gcccagcagg
     6481 ctgttctcca gcagcacatc aatagtctaa catactcacc ttttggagac tctcctctct
     6541 tccggaatcc gatgtcagac cctaagaaga aggaagagag attgaaacca acaaatccag
     6601 cagcccagaa ggctcttact acacctactc attataaact gacaccccgc cctgccacta
     6661 gagtccggcc aaaggcttta caaacaacag gcacagccaa gtcacatctc tttgatgggc
     6721 tggatgacga tgaaccatcc ctagccaatg gagcattcat gcccaagaag agcattaaga
     6781 agttggtttt gaagaacctt aataatagca atctcttttc tcctgttaat cgtgattcag
     6841 aaaatctagc ttcaccatct gaatatccag aaaatggaga gagatttagt ttcctaagca
     6901 aacctgttga tgagaatcgc cgctcgggcc cggccctggg gacagggcgg gctaggggcg
     6961 ccccagagtc catgatggaa gagaagaggc gaaaatactc catcagcagt gacaactctg
     7021 acaccactga cagtcatgcg acatctacat ccgcatcaag atgctccaaa ctgcccagca
     7081 gcaccaagtc gggctggccc cgacagaacg aaaagaagcc ctccgaggtt ttccggacag
     7141 acttgatcac agccatgaag atcccggact cataccagct cagcccggat gactactaca
     7201 tcctggcaga cccatggcga caggaatggg agaaaggtgt gcaggtgcct gccggggcag
     7261 aggccatccc agagcccgtg gtgaggatcc tcccaccact ggaaggcccc cctgcccagg
     7321 catccccgag cagcaccatg cttggtgagg gctcccagcc tgattggcca gggggcagcc
     7381 gctatgactt ggacgagatt gatgcctact ggctggagct catcaactcg gagcttaagg
     7441 agatggagag gccggagctg gacgagctga cattagagcg tgtgctggag gagctggaga
     7501 ccctgtgcca ccagaatatg gccagggcca ttgagacgca ggaggggctg ggcatcgagt
     7561 acgacgagga tgttgtctgc gacgtgtgtc gctctcctga gggcgaggat ggcaacgaga
     7621 tggtcttctg tgacaagtgc aacgtctgtg tgcatcaggc atgctacggg atcctcaagg
     7681 tgcccacggg cagctggctg tgccggacgt gtgccctggg tgtccagcca aagtgcctgc
     7741 tctgccccaa gcgaggagga gccttgaagc ccactagaag tgggaccaag tgggtgcatg
     7801 tcagctgtgc cctatggatt cctgaggtca gcatcggctg cccagagaag atggagccca
     7861 tcaccaagat ctcgcatatc ccagccagcc gctgggctct gtcctgcagc ctctgcaagg
     7921 aatgcacagg cacctgcatc cagtgttcca tgccttcctg cgtcacagcg ttccatgtca
     7981 catgcgcctt tgaccacggc ctggaaatgc ggactatatt agcagacaac gatgaggtca
     8041 agttcaagtc attctgccag gagcacagtg acgggggccc acgtaatgag cccacatctg
     8101 agcccacgga acccagccag gctggcgagg acctggaaaa ggtgaccctg cgcaagcagc
     8161 ggctgcagca gctagaggag gacttctacg agctggtgga gccggctgag gtggctgagc
     8221 ggctggacct ggctgaggca ctggtcgact tcatctacca gtactggaag ctgaagagga
     8281 aagccaatgc caaccagccg ctgctgaccc ccaagaccga cgaggtggac aacctggccc
     8341 agcaggagca ggacgtcctc taccgccgcc tgaagctctt cacccatctg cggcaggacc
     8401 tagagagggt tagaaatctg tgctacatgg tgacaaggcg cgagagaacg aaacacgcca
     8461 tctgcaaact ccaggagcag atattccacc tgcagatgaa acttattgaa caggatctgt
     8521 gtcgaggcct gtccacctca ttccccatcg atggcacctt cttcaacagc tggctggcac
     8581 agtcggtgca gatcacagca gagaacatgg ccatgagcga gtggccactg aacaatgggc
     8641 accgcgagga ccctgctcca gggctgctgt cagaggaact gctgcaggac gaggagacac
     8701 tgctcagctt catgcgggac ccctcgctgc gacctggtga ccctgctagg aaggcccgag
     8761 gccgcacccg cctgcctgcc aagaagaaac caccaccacc accaccgcag gacgggcctg
     8821 gttcacggac gactccagac aaagccccca agaagacctg gggccaggat gcaggcagtg
     8881 gcaagggggg tcaagggcca cctaccagga agccaccacg tcggacatct tctcacttgc
     8941 cgtccagccc tgcagccggg gactgtccca tcctagccac ccctgaaagc cccccgccac
     9001 tggcccctga gaccccggac gaggcagcct cagtagctgc tgactcagat gtccaagtgc
     9061 ctggccctgc agcaagccct aagcctttgg gccggctccg gccaccccgc gagagcaagg
     9121 taacccggag attgccgggt gccaggcctg atgctgggat gggaccacct tcagctgtgg
     9181 ctgagaggcc caaggtcagc ctgcattttg acactgagac tgatggctac ttctctgatg
     9241 gggagatgag cgactcagat gtagaggccg aggacggtgg ggtgcagcgg ggtccccggg
     9301 aggcaggggc agaggaggtg gtccgcatgg gcgtactggc ctcctagctc gagtgcggcc
     9361 gcaacccagc tttcttgtac aaagtggtga tatcggtacc gagctcgaat tctaccgggt
     9421 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     9481 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     9541 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     9601 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     9661 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     9721 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     9781 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     9841 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     9901 atctccgggc ctttcgacca tctagatcca ccgcgggcca ccatggtgag caagggcgag
     9961 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
    10021 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
    10081 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
    10141 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
    10201 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
    10261 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
    10321 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
    10381 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
    10441 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
    10501 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
    10561 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
    10621 tccaccggcg gcatggacga gctgtacaag tagagcggcc aactcgaggg atcccccggg
    10681 gtcgactgat caaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
    10741 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
    10801 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
    10861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10981 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
    11041 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
    11101 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    11161 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
    11221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    11341 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
    11401 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
    11461 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
    11521 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
    11581 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    11641 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11701 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    11761 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11821 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11881 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11941 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    12001 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    12061 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    12121 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    12181 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    12241 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tggg
//
