LOCUS       sequence-475551-       14672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus 
            of cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14672)
  AUTHORS   Han M, Fu ML, Zhu Y, Choi AA, Li E, Bezney J, Cai S, Miles L, Ma Y, 
            Qi LS
  TITLE     Programmable control of spatial transcriptome in live cells and 
            neurons.
  JOURNAL   Nature. 2025 Jul;643(8070):241-251. doi: 10.1038/s41586-025-09020-z.
            Epub 2025 May 21.
  PUBMED    40399675
REFERENCE   2  (bases 1 to 14672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-025-09020-z"; journalName: "Nature"; date: 
            "2025-07"; volume: "643"; issue: "8070"; pages: "241-251"
COMMENT     Sequence Label: pSLQ10531
FEATURES             Location/Qualifiers
     source          1..14672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             483..524
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    565..1153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(618..638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1036..1047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1156..1172)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1157..1173)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1263..1496
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1521..1539)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1522..1539)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1829..1848)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2062..2081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2181..2203
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2241..2259)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2327..2431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2432..2500,2501..3292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2650..2669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3463..4051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3952..3971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4205..4222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4292..4312)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4297..4626
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4477..4612
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5760..5825
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5955..5975
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6400..6534
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6437..6456)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6491..6510
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6702..7335
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7382..7507
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8004..8237
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8422..8466
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8751..8868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8941..9152
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          9153..10091
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10048..10068
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      10106..10115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10115..10138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             10154..10174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(13496..13498,13499..14209)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(13496..13498,13499..14209)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(13538..13559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(13799..13818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14146..14167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cacttccatt atccgttgac cataaaccgg atagagaaga tgaagctgcg aggattgaag
       61 ccgcaggagg gaaagtgatt cagtggaatg gagctcgtgt tttcggtgtt ctcgccatgt
      121 cgagatccat tggcgataga tacttgaaac catccatcat tcctgatccg gaagtgacgg
      181 ctgtgaagag agtaaaagaa gatgattgtc tgattttggc gagtgacggg gtttgggatg
      241 taatgacgga tgaagaagcg tgtgagatgg caaggaagcg gattctcttg tggcacaaga
      301 aaaacgcggt ggctggggat gcatcgttgc tcgcggatga gcggagaaag gaagggaaag
      361 atcctgcggc gatgtccgcg gctgagtatt tgtcaaagct ggcgatacag agaggaagca
      421 aagacaacat aagtgtggtg gtggttgatt tgaagtcaag cgctgcaggt gctaccacta
      481 gtggcaagcc catccccaac cccctgctgg gcctggacag cacctaacct gcaggcatgc
      541 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
      601 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
      661 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
      721 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
      781 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
      841 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
      901 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
      961 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     1021 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     1081 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     1141 gccgcctccc cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt
     1201 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     1261 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     1321 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1381 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1441 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     1501 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     1561 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     1621 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     1681 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     1741 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     1801 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     1861 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     1921 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     1981 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     2041 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     2101 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2161 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2221 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     2281 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4081 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4141 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4201 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4261 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
     4321 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4381 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4441 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4501 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4561 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4621 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
     4681 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
     4741 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
     4801 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
     4861 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
     4921 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
     4981 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
     5041 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
     5101 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
     5161 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
     5221 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
     5281 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
     5341 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
     5401 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
     5461 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
     5521 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
     5581 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
     5641 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
     5701 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     5761 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     5821 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     5881 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     5941 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     6001 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     6061 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     6121 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     6181 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     6241 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     6301 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     6361 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     6421 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6481 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
     6541 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
     6601 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     6661 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     6721 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6781 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     6841 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     6901 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     6961 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     7021 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     7081 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     7141 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7201 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7261 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7321 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7381 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7441 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7501 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7561 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7621 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7681 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     7741 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     7801 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     7861 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7921 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7981 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     8041 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     8101 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8161 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8221 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8281 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8341 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8401 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8461 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8521 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8581 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8641 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     8701 ttagtgaacg gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga
     8761 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     8821 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     8881 agggacagca gagatccagt ttggatcgat aagcttgata tcgaattcgt gcccgtcagt
     8941 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     9001 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     9061 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     9121 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     9181 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     9241 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     9301 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     9361 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     9421 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     9481 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     9541 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     9601 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     9661 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     9721 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     9781 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     9841 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg cgcttttgga
     9901 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     9961 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    10021 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    10081 ttccatttca ggaattctca cgcgtgccac catggattac aaggacgacg atgacaaggg
    10141 atccggcgga ccgcccaaaa agaagaggaa agtgggatcc ggtaccaaca tccccgctct
    10201 ggtggaaaac cagaagaagt actttggcac ctacagcgtg atggccatgc tgaacgctca
    10261 gaccgtgctg gaccacatcc agaaggtggc cgatattgag ggcgagcaga acgagaacaa
    10321 cgagaatctg tggtttcacc ccgtgatgag ccacctgtac aacgccaaga acggctacga
    10381 caagcagccc gagaaaacca tgttcatcat cgagcggctg cagagctact tcccattcct
    10441 gaagatcatg gccgagaacc agagagagta cagcaacggc aagtacaagc agaaccgcgt
    10501 ggaagtgaac agcaacgaca tcttcgaggt gctgaagcgc gccttcggcg tgctgaagat
    10561 gtacagggac ctgaccaacg catacaagac ctacgaggaa aagctgaacg acggctgcga
    10621 gttcctgacc agcacagagc aacctctgag cggcatgatc aacaactact acacagtggc
    10681 cctgcggaac atgaacgaga gatacggcta caagacagag gacctggcct tcatccagga
    10741 caagcggttc aagttcgtga aggacgccta cggcaagaaa aagtcccaag tgaataccgg
    10801 attcttcctg agcctgcagg actacaacgg cgacacacag aagaagctgc acctgagcgg
    10861 agtgggaatc gccctgctga tctgcctgtt cctggacaag cagtacatca acatctttct
    10921 gagcaggctg cccatcttct ccagctacaa tgcccagagc gaggaacggc ggatcatcat
    10981 cagatccttc ggcatcaaca gcatcaagct gcccaaggac cggatccaca gcgagaagtc
    11041 caacaagagc gtggccatgg atatgctcaa cgaagtgaag cggtgccccg acgagctgtt
    11101 cacaacactg tctgccgaga agcagtcccg gttcagaatc atcagcgacg accacaatga
    11161 agtgctgatg aagcggagca gcgacagatt cgtgcctctg ctgctgcagt atatcgatta
    11221 cggcaagctg ttcgaccaca tcaggttcca cgtgaacatg ggcaagctga gatacctgct
    11281 gaaggccgac aagacctgca tcgacggcca gaccagagtc agagtgatcg agcagcccct
    11341 gaacggcttc ggcagactgg aagaggccga gacaatgcgg aagcaagaga acggcacctt
    11401 cggcaacagc ggcatccgga tcagagactt cgagaacatg aagcgggacg acgccaatcc
    11461 tgccaactat ccctacatcg tggacaccta cacacactac atcctggaaa acaacaaggt
    11521 cgagatgttt atcaacgaca aagaggacag cgccccactg ctgcccgtga tcgaggatga
    11581 tagatacgtg gtcaagacaa tccccagctg ccggatgagc accctggaaa ttccagccat
    11641 ggccttccac atgtttctgt tcggcagcaa gaaaaccgag aagctgatcg tggacgtgca
    11701 caaccggtac aagagactgt tccaggccat gcagaaagaa gaagtgaccg ccgagaatat
    11761 cgccagcttc ggaatcgccg agagcgacct gcctcagaag atcctggatc tgatcagcgg
    11821 caatgcccac ggcaaggatg tggacgcctt catcagactg accgtggacg acatgctgac
    11881 cgacaccgag cggagaatca agagattcaa ggacgaccgg aagtccattc ggagcgccga
    11941 caacaagatg ggaaagagag gcttcaagca gatctccaca ggcaagctgg ccgacttcct
    12001 ggccaaggac atcgtgctgt ttcagcccag cgtgaacgat ggcgagaaca agatcaccgg
    12061 cctgaactac cggatcatgc agagcgccat tgccgtgtac gatagcggcg acgattacga
    12121 ggccaagcag cagttcaagc tgatgttcga gaaggcccgg ctgatcggca agggcacaac
    12181 agagcctcat ccatttctgt acaaggtgtt cgcccgcagc atccccgcca atgccgtcga
    12241 gttctacgag cgctacctga tcgagcggaa gttctacctg accggcctgt ccaacgagat
    12301 caagaaaggc aacagagtgg atgtgccctt catccggcgg gaccagaaca agtggaaaac
    12361 acccgccatg aagaccctgg gcagaatcta cagcgaggat ctgcccgtgg aactgcccag
    12421 acagatgttc gacaatgaga tcaagtccca cctgaagtcc ctgccacaga tggaaggcat
    12481 cgacttcaac aatgccaacg tgacctatct gatcgccgag tacatgaaga gagtgctgga
    12541 cgacgacttc cagaccttct accagtggaa ccgcaactac cggtacatgg acatgcttaa
    12601 gggcgagtac gacagaaagg gctccctgca gcactgcttc accagcgtgg aagagagaga
    12661 aggcctctgg aaagagcggg cctccagaac agagcggtac agaaagcagg ccagcaacaa
    12721 gatccgcagc aaccggcaga tgagaaacgc cagcagcgaa gagatcgaga caatcctgga
    12781 taagcggctg agcaacagcc ggaacgagta ccagaaaagc gagaaagtga tccggcgcta
    12841 cagagtgcag gatgccctgc tgtttctgct ggccaaaaag accctgaccg aactggccga
    12901 tttcgacggc gagaggttca aactgaaaga aatcatgccc gacgccgaga agggaatcct
    12961 gagcgagatc atgcccatga gcttcacctt cgagaaaggc ggcaagaagt acaccatcac
    13021 cagcgagggc atgaagctga agaactacgg cgacttcttt gtgctggcta gcgacaagag
    13081 gatcggcaac ctgctggaac tcgtgggcag cgacatcgtg tccaaagagg atatcatgga
    13141 agagttcaac aaatacgacc agtgcaggcc cgagatcagc tccatcgtgt tcaacctgga
    13201 aaagtgggcc ttcgacacat accccgagct gtctgccaga gtggaccggg aagagaaggt
    13261 ggacttcaag agcatcctga aaatcctgct gaacaacaag aacatcaaca aagagcagag
    13321 cgacatcctg cggaagatcc ggaacgcctt cgatgcaaac aattaccccg acaaaggcgt
    13381 ggtggaaatc aaggccctgc ctgagatcgc catgagcatc aagaaggcct ttggggagta
    13441 cgccatcatg aaggcggccg cggagggagc tctagaggcg ctaccggtcg ccaccgtgag
    13501 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
    13561 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
    13621 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
    13681 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
    13741 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
    13801 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
    13861 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
    13921 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
    13981 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
    14041 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
    14101 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
    14161 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc atatgggatc
    14221 cggatccacg cgtgtgcctt tgtatggttt tacttcgatt tgtggaagaa gacctgagat
    14281 ggaagctgct gtttcgacta taccaagatt ccttcaatct tcctctggtt cgatgttaga
    14341 tggtcggttt gatcctcaat ccgccgctca tttcttcggt gtttacgacg gccatggcgg
    14401 ttctcaggta gcgaactatt gtagagagag gatgcatttg gctttggcgg aggagatagc
    14461 taaggagaaa ccgatgctct gcgatggtga tacgtggctg gagaagtgga agaaagctct
    14521 tttcaactcg ttcctgagag ttgactcgga gattgagtca gttgcgccgg agacggttgg
    14581 gtcaacgtcg gtggttgccg ttgttttccc gtctcacatc ttcgtcgcta actgcggtga
    14641 ctctagagcc gttctttgcc gcggcaaaac tg
//
