LOCUS       sequence-475552-        8412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This virus-induced gene silencing (VIGS) reporter vector is used to 
            evaluate the silencing of GhPDS gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVSr-GhPDS
FEATURES             Location/Qualifiers
     source          1..8412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        618..963
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     906..926
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             982..1917
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1972..2148
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     2297..2319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2311..2328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2312..2328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2338..2357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2338..2356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2381..2397
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2382..2398
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2407..4116
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          3155..4116
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     CDS             4504..6156
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4570..4588)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6218..6394
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6404..6428
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6519..7107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6599..6618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7278..8093)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7278..8093)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8001..8020
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8085..8104
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      join(8384..8412,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcgtacccc tactccaaaa atgtcaaaga tacagtctca
      601 gaagaccaaa gggctattga gacttttcaa caaagggtaa tttcgggaaa cctcctcgga
      661 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc
      721 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatgcctc tgccgacagt
      781 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      841 acgtcttcaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa
      901 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
      961 acagcccaag ctctagccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
     1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
     1921 tagggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     1981 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     2041 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     2101 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatcga taacattaac
     2161 gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2221 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2281 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     2341 tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcga cggtatcgat
     2401 aagcttattc gggtcaaggc ggaagccagc gcgccacccc acgtcagcaa atacggaggc
     2461 gcggggttga cggcgtcacc cggtcctaac ggcgaccaac aaaccagcca gaagaaatta
     2521 cagtaaaaaa aaagtaaatt gcactttgat ccacctttta ttacctaagt ctcaatttgg
     2581 atcaccctta aacctatctt ttcaatttgg gccgggttgt ggtttggact accatgaaca
     2641 acttttcgtc atgtctaact tccctttcag caaacatatg aaccatatat agaggagatc
     2701 ggccgtatac tagagctgat gtgtttaagg tcgttgattg cacgagaaaa aaaaatccaa
     2761 atcgcaacaa tagcaaattt atctggttca aagtgaaaag atatgtttaa aggtagtcca
     2821 aagtaaaact tatagataat aaaatgtggt ccaaagcgta attcactcaa aaaaaatcaa
     2881 cgagacgtgt accaaacgga gacaaacggc atcttctcga aatttcccaa ccgctcgctc
     2941 gcccgcctcg tcttcccgga aaccgcggtg gtttcagcgt ggcggattct ccaagcagac
     3001 ggagacgtca cggcacggga ctcctcccac cacccaaccg ccataaatac cagccccctc
     3061 atctcctctc ctcgcatcag ctccaccccc gaaaaatttc tccccaatct cgcgaggctc
     3121 tcgtcgtcga atcgaatcct ctcgcgtcct caaggtacgc tgcttctcct ctcctcgctt
     3181 cgtttcgatt cgatttcgga cgggtgaggt tgttttgttg ctagatccga ttggtggtta
     3241 gggttgtcga tgtgattatc gtgagatgtt taggggttgt agatctgatg gttgtgattt
     3301 gggcacggtt ggttcgatag gtggaatcgt ggttaggttt tgggattgga tgttggttct
     3361 gatgattggg gggaattttt acggttagat gaattgttgg atgattcgat tggggaaatc
     3421 ggtgtagatc tgttggggaa ttgtggaact agtcatgcct gagtgattgg tgcgatttgt
     3481 agcgtgttcc atcttgtagg ccttgttgcg agcatgttca gatctactgt tccgctcttg
     3541 attgagttat tggtgccatg ggttggtgca aacacaggct ttaatatgtt atatctgttt
     3601 tgtgtttgat gtagatctgt agggtagttc ttcttagaca tggttcaatt atgtagcttg
     3661 tgcgtttcga tttgatttca tatgttcaca gattagataa tgatgaactc ttttaattaa
     3721 ttgtcaatgg taaataggaa gtcttgtcgc tatatctgtc ataatgatct catgttacta
     3781 tctgccagta atttatgcta agaactatat tagaatatca tgttacaatc tgtagtaata
     3841 tcatgttaca atctgtagtt catctatata atctattgtg gtaatttctt tttactatct
     3901 gtgtgaagat tattgccact agttcattct acttatttct gaagttcagg atacgtgtgc
     3961 tgttactacc tatctgaata catgtgtgat gtgcctgtta ctatcttttt gaatacatgt
     4021 atgttctgtt ggaatatgtt tgctgtttga tccgttgttg tgtccttaat cttgtgctag
     4081 ttcttaccct atctgtttgg tgattatttc ttgcagatag ttatcggatc catccggggg
     4141 gcaatgagat ccttgcctga agactggaga gagatttcat acttcaagaa attagagaaa
     4201 ttagttggag ttccagttat caacgttcac atctggtttg ataggaaatt gaagaacacc
     4261 tatgatcatc tactctttag cagaagcccg cttttaagtg tttatgctga catgtctgta
     4321 acatgtaagg aatattacaa tccaaaccaa tccatgttgg agttagtttt tgccccagca
     4381 gaagaatgga ttgcatgtag tgactcagaa attattgatg ctacaatgaa ggaacttgca
     4441 aagctctttc ctgatgaaat atctgcagat cagagtaaag caatggtgag caagggcgag
     4501 gagatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     4561 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     4621 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     4681 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     4741 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     4801 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     4861 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     4921 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     4981 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     5041 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     5101 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     5161 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     5221 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     5281 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     5341 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     5401 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     5461 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     5521 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     5581 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     5641 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     5701 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     5761 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     5821 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     5881 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     5941 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     6001 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     6061 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     6121 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagaattcg gtacgctgaa
     6181 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
     6241 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     6301 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     6361 cctaaaacca aaatccagta ctaaaatcca gatccactag ccttgacagg atatattggc
     6421 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
     6481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag agttggtagc
     7021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgtg
     7261 taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     7321 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     7381 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     7441 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     7501 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     7561 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     7621 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     7681 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     7741 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg
     7801 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     7861 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     7921 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg gtagattgtc
     7981 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     8041 ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct cataacaccc
     8101 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     8161 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     8221 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     8281 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     8341 tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc
     8401 gtcgagcggg ct
//
