LOCUS       sequence-475555-       13365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one plant virus expression vector for virus-induced genome 
            editing (VIGE)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVEe
FEATURES             Location/Qualifiers
     source          1..13365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        4231..4306
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(6451..6468)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6451..6467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6460..6482)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6670..6694
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7995..8624
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9053..10126
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10192..10386
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10622..10644)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10730..10870
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10785..10804
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10885..10902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11056..11644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11136..11155)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11731..12525)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12950..12974
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13242..13263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13278..13283,13284..13301,13302..13308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13316..13332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13321..13343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13340..13356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13340..13356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgt tgaacaacgg
     3781 aaactcgact tgccttccgc acaatacatc atttcttctt agcttttttt cttcttcttc
     3841 gttcatacag tttttttttg tttatcagct tacattttct tgaaccgtag ctttcgtttt
     3901 cttcttttta actttccatt cggagttttt gtatcttgtt tcatagtttg tcccaggatt
     3961 agaatgatta ggcatcgaac cttcaagaat ttgattgaat aaaacatctt cattcttaag
     4021 atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt
     4081 atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca
     4141 aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa
     4201 gtagtgattc gagacctctt acacggtctc gttttagagc tagaaatagc aagttaaaat
     4261 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttttctcga
     4321 gattcgctag cgttaactgg ccataatcct gtgtatgcaa cgttgaaaat tcggatctat
     4381 ttttacgatt cgatcatgaa ttaataaaat ttaaatttta ttgaatgatt ttctagtaca
     4441 taactgacat acgacttatc agttgcgaaa cgaacagctc taattacatt gttaagtgaa
     4501 attacgccta attcgtctaa gtacaacata accaactgtt taaacctaat taaataagtc
     4561 gacccagaag ctgtcagaga tgtcgtccag acttggaagt tcaggtaagc tttgtggaga
     4621 tgcaacgctc tccgtaagtt gtggttgaat cggatctgga cgtggtatat acgcgtcctg
     4681 gtgtatagca gatcctctac tccgtatatc ttgaaatata ggggatttat tatctcccag
     4741 atatacacgc cattctctgc ctgacgtgca gtgatgattt cccctgtgcg tgaatccatg
     4801 accggtgcag cctatgtgga agtatatggt gcacccacag tttagatcaa tgcgtcgtcg
     4861 tctaatcgcc ctcctcttgg cttgcctgtg tgccttcttg atagaggggg gcgtcgagag
     4921 tgatgaagat aacattcttg agagtccaat gtttgagcga cgcattttcc gctttatcca
     4981 ggaaatcttt atagctggat ccctcgcctg gattgcaaag cacgattgaa gggatccctc
     5041 ctttaatttg aactggtttc ccgtacttgc aatttgactg ccagtctttt tgggccccaa
     5101 gaagctcttt ccaatgcttt agctttaggt attgcggtgc tacgtcatca atgacgttat
     5161 aggcaacatt attcgagtac actcgattat tgaagtccag gtgtccactg aggtaattat
     5221 gtgggcctaa agcacgagcc cacatcgtct tgcccgttct agaatcacct tccactatga
     5281 gactaactgg tcttacaggc cgcgcagcgg gacccattcc aaaataatca tccgcccatt
     5341 cttgcatctg ttcaggaacg ttagtgaaag aggaaagtgg aaatggaggg acccattgtt
     5401 caggcgcctt tttgaaaagg cgttccaagt tcgctgtaat gttatggtaa ctcacgatga
     5461 acgcccttgg atctccggcc cggataattg caagagcttc cgccaaacta ggtgcattga
     5521 tggcgttata atagacgtcg tctttattct tctttgtacc cccaggcact ttgaactgtc
     5581 cggattcaca acaatcgccg tctttggtga tgtaattctt gacggcgttg gcgtctttgg
     5641 ctgcttgtat gtttgggtga aaattggtcg accttctggg gtgagtaagg tcgaaaaatc
     5701 tcttattctt gatgttggac tttccagaga gttggatgag acagtgaagg tgtgggaatc
     5761 cgtccgagtg ctcctctctg gcaactcgaa tatatgttgg tttgacgact gaccatgata
     5821 aggtttgaag catttgaaga gcttcatttt ttgggatatc gcacttggga taggttaaga
     5881 aaatatgtct ggcttgtaaa cgaaaagaat cggggtttct aggcattttt taaatagtga
     5941 cccaggactc aaaaactagc tctcaacttc tctcatatgt tgtggagtct ggagtccttt
     6001 atatatgttt acgctataga aggcatttgg ggactctccc gggcccacca taggactcca
     6061 taccgcggcc atctatataa tattactgat ggccgcgcgg attttggagc ccccttttgt
     6121 cgttagcgcg acctttaatt caaattaaag gttgcttttt tctatcgtcc aatcattttg
     6181 cgcctgacta gcctagatat tagcaacaac ttcgcgacta agttgttgac cgtcaagtat
     6241 aaattaatgg gcttatggcc caatgggtat agagcaaaat gcccaagcgt gatgccccgt
     6301 ggcgcttatt agcgggtacg tctaaggtaa gtcgaaatgc caactactct cccttgagag
     6361 tattgggccc taaagttaat aagggacctc gagggggggc ccggtacccg gggatcctct
     6421 agagtcgacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
     6481 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6541 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6601 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     6661 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     6721 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     6781 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     6841 tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc
     6901 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     6961 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     7021 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     7081 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     7141 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     7201 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     7261 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     7321 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     7381 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     7441 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     7501 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     7561 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     7621 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     7681 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     7741 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca
     7801 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     7861 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     7921 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     7981 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     8041 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     8101 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     8161 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     8221 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     8281 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     8341 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     8401 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     8461 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     8521 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     8581 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     8641 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     8701 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     8761 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     8821 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     8881 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     8941 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     9001 ttgcctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg
     9061 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     9121 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     9181 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     9241 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     9301 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     9361 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     9421 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     9481 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     9541 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     9601 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     9661 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     9721 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     9781 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     9841 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     9901 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     9961 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
    10021 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
    10081 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
    10141 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtgtctttc ctgtggatag
    10201 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
    10261 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
    10321 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
    10381 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
    10441 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
    10501 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
    10561 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
    10621 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
    10681 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
    10741 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
    10801 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
    10861 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    10921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    10981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
    11521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    11581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    11641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    11701 ttaagggatt ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata
    11761 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    11821 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    11881 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    11941 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    12001 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    12061 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    12121 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    12181 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    12241 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    12301 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    12361 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    12421 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    12481 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    12541 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    12601 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    12661 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    12721 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    12781 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    12841 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    12901 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
    12961 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
    13021 tgtactgaat taacgccgaa ttaattcggg gatagccctt tggtcttctg agactgtatc
    13081 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
    13141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    13201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    13261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    13321 agcggataac aatttcacac aggaaacagc tatgacatga ttacg
//
