LOCUS       sequence-475624-       10145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA-tagged NUP98::LNP1 in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 10145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CCLMPC_HA-NUP98::LNP1
FEATURES             Location/Qualifiers
     source          1..10145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      919..1507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1408..1427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1661..1678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1795..1816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1831..1836,1837..1854,1855..1861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1869..1885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1874..1896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1893..1909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1893..1909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1928..1948
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1930..1948
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2049..2428
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2429..2632
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2583..2603
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2647..2827
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2874..2999
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3492..3725
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3910..3954
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4251..4368
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4971..4995
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5019..5045
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6631..6648)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(7228..7252)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7281..7780
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7316..7339)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7713..7732
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7801..7810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7807..8517
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7952..7970)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8220..8239
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             8639..8872
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8944..9065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8981..9000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9035..9054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9105..9240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9260..9279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9261..9279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9289..9306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9289..9305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9298..9320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9447..9902
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9534..9553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9744..9765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9928..10032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10033..10101,10102..10145,1..748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
       61 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      601 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1741 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1801 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1861 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     1921 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttggccatt
     1981 gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc
     2041 gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     2101 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2161 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     2221 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     2281 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     2341 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     2401 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     2461 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     2521 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     2581 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
     2641 gaaccggggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     2701 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     2761 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     2821 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     2881 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     2941 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     3001 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     3061 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     3121 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     3181 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     3241 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     3301 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     3361 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     3421 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     3481 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     3541 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     3601 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     3661 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     3721 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     3781 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     3841 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     3901 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     3961 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     4021 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     4081 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     4141 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     4201 tctcgacggt atcgataagc taattcacaa atggcagtat tcatccacaa ttttaaaaga
     4261 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     4321 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     4381 agggacagca gagatccagt ttgggaatta gcttgatcga ttagtccaat ttgttaaaga
     4441 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
     4501 agagtacgag ccatagatag aataaaagat tttatttagt ctccagaaaa aggggggaat
     4561 gaaagacccc acctgtaggt ttggcaagct aggatcaagg ttaggaacag agagacagca
     4621 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     4681 acagttggaa cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     4741 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     4801 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     4861 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     4921 aagagcccac aacccctcac tcggcgcgat ctagatctcg aatcgaattc acaagtttgt
     4981 acaaaaaagc aggctccgcg gccgccccct tcaccatgta cccatacgat gttccagatt
     5041 acgcttttaa caaatcattt ggaacaccct ttgggggtgg cacaggtggc tttggcacaa
     5101 cttcaacatt tggacagaat actggctttg gcactactag tggaggggca tttggaacat
     5161 ctgcatttgg ttctagcaac aatactggag gcctctttgg aaattcacag actaaaccag
     5221 gaggattgtt tggaaccagt tcatttagcc agccagctac ctccacaagc actggctttg
     5281 ggtttggtac gtcaacagga acagcaaata ccttgtttgg aactgcaagc acagggacca
     5341 gtctcttctc atcccaaaac aatgcctttg cacaaaataa accaactggc tttggcaatt
     5401 ttggaaccag tactagcagt ggaggactct ttggaaccac aaataccacc tctaatcctt
     5461 ttggcagcac atctggctcc ctctttgggc caagtagttt tacagctgct cctactggga
     5521 ctactattaa atttaaccct ccaactggta cagatactat ggtcaaagct ggagttagca
     5581 ctaacataag taccaagcac cagtgtatta ctgctatgaa agaatatgaa agcaagtcac
     5641 tagaggaact tcgtttagag gattatcagg ctaacaggaa gggcccacag aaccaggtgg
     5701 gagcaggtac cacaactggc ttgtttgggt cttctccagc cacttccagc gcaacaggac
     5761 tcttcagctc ctccaccact aattcaggct ttgcatatgg tcagaacaaa actgcctttg
     5821 gaactagtac aactggattt ggaacaaatc caggtggtct ctttggccaa cagaatcagc
     5881 agactaccag cctcttcagc aaaccatttg gccaggctac aaccacccag aacactggct
     5941 tttcctttgg taataccagc accataggac agccaagcac caacaccatg ggattatttg
     6001 gagtaaccca agcctcacag cctggaggtc tttttgggac agctacaaac accagcactg
     6061 ggacagcatt tggaacagga acaggtctct ttgggcagac caatactgga tttggtgctg
     6121 ttggttcgac cctgtttggc aataacaagc ttactacatt tggaagcagc acaaccagtg
     6181 caccttcatt tggtacaacc agtggcgggc tctttggttt tggcacaaat accagtggga
     6241 atagtatttt tggaagtaaa ccagcacctg ggactcttgg aactgggctt ggtgcaggat
     6301 ttggaacagc tcttggtgct ggacaggcat ctttgtttgg gaacaaccaa cctaagattg
     6361 gagggcctct tggtacagga gcctttgggg cccctggatt taatactacg acagccactt
     6421 tgggctttgg agccccccag gccccagtag ctttgacaga tccaaatgct tctgctgccc
     6481 agcaggctgt tctccagcag cacatcaata gtctaacata ctcacctttt ggagactctc
     6541 ctctcttccg gaatccgatg tcagacccta agaagaagga agagggacag gccttcagta
     6601 tttggcatca cctttacatg gagcacaaag atgatgatga tgatgatgtg tcttttgcca
     6661 aatggatgag cagcttctgg ggccacagct ggagagagga ggatcagaga ggactccggg
     6721 aacgccaccg actgcaagcc accagtcaca ggaaaacctc cctgccctgc ccacttcctg
     6781 tgcttcccag aattccatca tctgactgcc atcctagaag gcattctcat gaggaccagg
     6841 aattccgatg ccgatcgtct gaccgtcttc ctagaaggca ttctcatgag gaccaagaat
     6901 ttcgatgccg tagccacgta cgggattaca gaaaatactc agaggatggg tcattcaagg
     6961 agccactgga atcaaaagga agatcccatt ccaaaattga gaaattttca gagtcctttg
     7021 aacggcaact gtgctttaga accaagcgtt ctgcctcttt gggacctgaa agcagaaagg
     7081 agagaaatga aagagaatgc ctgaggatgg agataaaatc ccgaaagaaa gtagaggaag
     7141 aaaggagctc taggaaagaa gaacatggag aagcacacat ggctcccctg tttgaaaaag
     7201 ggcctgaata aaagggtggg cgcgccgacc cagctttctt gtacaaagtg gtgatatcgg
     7261 taccgagctc gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     7321 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     7381 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     7441 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     7501 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     7561 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     7621 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     7681 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     7741 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg accatctaga tccaccgcgg
     7801 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     7861 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     7921 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     7981 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     8041 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     8101 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     8161 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     8221 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     8281 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     8341 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     8401 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     8461 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagagc
     8521 ggccaactcg agggatcccc cggggtcgac tgatcaaatt cgagctcggt acctttaaga
     8581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8641 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8701 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8761 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8821 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8881 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8941 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9061 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     9121 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9181 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9241 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     9301 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9361 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9421 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9481 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9541 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9601 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9661 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9721 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9781 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9841 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9901 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     9961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10141 cagtt
//
