LOCUS       sequence-475626-        9915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1263
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     RBS             2964..2972
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    6643..6764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6680..6699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6734..6753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6771..7226)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6908..6929)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7120..7139
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7253..7357
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7359..7716
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7359..7550
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7382..7402)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7567..7702
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7751..8545
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7751..8545
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7805..7824)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8415..8434
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8733..8752)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8777..8824
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9153..9741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9642..9661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
      601 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
      661 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
      721 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
      781 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
      841 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
      901 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
      961 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     1021 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     1081 aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     1141 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     1201 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     1261 aattccggaa gtgctgggag cgcaagcgga agcggaggct ccggtgagga aacagctata
     1321 tgggaacaac atacagtgac gcttcacagg gctcctggat ttggatttgg aattgcaata
     1381 tctggtggac gagataatcc tcattttcag agtggggaaa cgtcaatagt gatttcagat
     1441 gtgctgaaag gaggaccagc tgaaggacag ctacaggaaa atgaccgagt tgcaatggtt
     1501 aacggagttt caatggataa tgttgaacat gcttttgctg ttcagcaact aaggaaaagt
     1561 gggaaaaatg caaaaattac aattagaagg aagaagaaag ttcaaatacc agtaagtcgt
     1621 cctgatcctg aaccagtatc tgataatgaa gaagatagtt atgatgagga aatacatgat
     1681 ccaagaagtg gccggagtgg tgtggttaac agaaggagtg agaagatttg gccgagggat
     1741 agaagtgcaa gtagagagag gagcttgtcc ccgcggtcag acaggcggtc agtggcttcc
     1801 agccagcctg ctaaacctac taaagtcaca ctggtgaaat cccggaaaaa tgaagaatat
     1861 ggtcttcgat tggcaagcca tatatttgtt aaggaaattt cacaagatag tttggcagca
     1921 agagatggca atattcaaga aggtgatgtt gtattgaaga taaatggtac tgtgacagaa
     1981 aatatgtcat tgacagatgc aaagacattg atagaaaggt ctaaaggcaa attaaaaatg
     2041 gtagttcaaa gagatgaacg ggctacgcta ttgaatgtcc ctgatctttc tgacagcatc
     2101 cactctgcta atgcctctga gagagacgac atttcagaaa ttcagtcact ggcatcagat
     2161 cattctggtc gatcacacga taggcctccc cgccgcagcc ggtcacgatc tcctgaccag
     2221 cggtcagagc cttctgatca ttccaggcac tcgccgcagc agccaagcaa tggcagtctg
     2281 cggagtagag atgaagagag aatttctaaa cctggggctg tctcaactcc tgtaaagcat
     2341 gctgatgatc acacacctaa aacagtggaa gaagttacag ttgaaagaaa tgagaaacaa
     2401 acaccttctc ttccagaacc aaagcctgtg tatgcccaag ttggcaacca gatgtggatt
     2461 tacctgtcag tccatctgat ggtgtcctac ctaattcaac tcatgaagat gggatttctt
     2521 cggcccagca tgaaattggt aaaattcaga aaaggagata gtgtgggttt gcggctggct
     2581 ggtggaaatg atgttggaat atttgtagct ggcgttctag aagatagccc tgcagccaag
     2641 gaaggcttag aggaaggtga tcaaattctc agggtaaaca acgtagattt tacaaatatc
     2701 ataagagaag aagccgtcct tttcctgctt gacctcccta aaggagaaga agtgaccata
     2761 ttggctcaga agaagaagga tgtttatcgt cgcattgtag aatcagatgt aggagattct
     2821 ttctatatta gaacccattt tgaatatgaa aaggaatctc cctatggact tagttttaac
     2881 aaaggagagg tgttccgtgc tgtggatacc ttgtacaatg gaaaactggg ctcttggctt
     2941 gctattcgaa ttggtaaaaa tcataaggag gtagaacgag gcatcatccc taataagaac
     3001 agagctgagc agctcgccag tgtacagtat acacttccaa aaacagcagg cggagaccgt
     3061 gctgacttct ggagattcag aggtcttcgc agctccaaga gaaatcttcg aaaaagcaga
     3121 gaggatttgt ccgctcagcc tgttcaaaca aagtttccag cttatgaaag agtggttctt
     3181 cgagaagctg gatttctgag gcctgtaacc atttttggac caatagctga tgttgccaga
     3241 gaaaagctgg caagagaaga accagatatt tatcaaattg caaagagtga accacgagac
     3301 gctggaactg accaacgtag ctctggctat attcgcctgc atacaataaa gcaaatcata
     3361 gatcaagaca aacatgcttt attagatgta acaccaaatg cagttgatcg tcttaactat
     3421 gcccagtggt atccaattgt tgtatttctt aaccctgatt ctaagcaagg agtaaaaaca
     3481 atgagaatga ggttatgtcc agaatctcgg aaaagtgcca ggaagttata cgagcgatct
     3541 cataaacttg ctaaaaataa tcaccatctt tttacaacta caattaactt aaattcaatg
     3601 aatgatggtt ggtatggtgc gctgaaagaa gcagttcaac aacagcaaaa ccagctggta
     3661 tgggtttccg agggaaaggc ggatggtgct acaagtgatg accttgattt gcatgatgat
     3721 cgtctgtcct acctgtcagc tccaggtagt gaatactcaa tgtatagcac ggacagtaga
     3781 cacacttctg actatgaaga cacagacaca gaaggcgggg cctacactga tcaagaacta
     3841 gatgaaactc ttaatgatga ggttgggact ccaccggagt ctgccattac acggtcctct
     3901 gagcctgtaa gagaggactc ctctggaatg catcatgaaa accaaacata tcctccttac
     3961 tcaccacaag cgcagccaca accaattcat agaatagact cccctggatt taagccagcc
     4021 tctcaacaga aagcagaagc ttcatctcca gtcccttacc tttcgcctga aacaaaccca
     4081 gcatcatcaa cctctgctgt taatcataat gtaaatttaa ctaatgtcag actggaggag
     4141 cccaccccag ctccttccac ctcttactca ccacaagctg attctttaag aacaccaagt
     4201 actgaggcag ctcacataat gctaagagat caagaaccat cattgtcgtc gcatgtagat
     4261 ccaacaaagg tgtatagaaa ggacccatat cccgaggaaa tgatgaggca gaaccatgtt
     4321 ttgaaacagc cagccgttag tcacccaggg cacaggccag acaaagagcc taatctgacc
     4381 tatgaacccc aactcccata cgtagagaaa caagccagca gagacctgga gcagcccaca
     4441 tacagatacg agtcctcaag ctatacggac cagttttctc gaaactatga acatcgtctg
     4501 cgatacgaag atcgcgtccc catgtatgaa gaacagtggt catattatga tgacaaacag
     4561 ccctacccat ctcggccacc ttttgataat cagcactctc aagaccttga ctccagacag
     4621 catcccgaag agtcctcaga acgagggtac tttccacgtt ttgaagagcc agcccctctg
     4681 tcttacgaca gcagaccacg ttacgaacag gcacctagag catccgccct gcggcacgaa
     4741 gagcagccag ctcctgggta tgacacacat ggtagactca gaccggaagc ccagccccac
     4801 ccttcagcag ggcccaagcc tgcagagtcc aagcagtatt ttgagcaata ttcacgcagt
     4861 tacgagcaag taccacccca aggatttacc tctagagcag gtcattttga gcctctccat
     4921 ggtgctgcag ctgtccctcc gctgatacct tcatctcagc ataagccaga agctctgcct
     4981 tcaaacacca aaccactgcc tccaccccca actcaaaccg aagaagagga agatccagca
     5041 atgaagccac agtctgtact caccagagtt aagatgtttg aaaacaaaag gtctgcatcc
     5101 ttagagacca agaaggatgt aaatgacact ggcagtttta agcctccaga agtagcatct
     5161 aaaccttcag gtgctcccat cattggtccc aaacccactt ctcagaatca attcagtgaa
     5221 catgacaaaa ctctgtacag gattccagaa cctcaaaaac ctcaactgaa gccacctgaa
     5281 gatattgttc ggtccaatca ttatgaccct gaagaagatg aagaatatta tcgaaaacag
     5341 ctgtcatact ttgaccgaag aagttttgag aataagcctc ctgcacacat tgctgccagc
     5401 catctctccg agcctgcaaa gccagctcat tctcagaatc aatcaaattt ttctagttat
     5461 tcttcaaagg gaaagcctcc tgaagctgat ggtgtggata gatcatttgg cgagaaacgc
     5521 tatgaaccca tccaggccac tccccctcct cctccattgc cctcgcagta tgcccagcca
     5581 tctcagcctg tcaccagcgc gtctctccac atacattcta agggagcaca tggtgaaggt
     5641 aattcagtgt cattggattt tcagaattcc ttagtgtcca aaccagaccc acctccatct
     5701 cagaataagc cagcaacttt cagaccacca aaccgagaag atactgctca ggcagctttc
     5761 tatccccaga aaagttttcc agataaagcc ccagttaatg gaactgaaca gactcagaaa
     5821 acagtcactc cagcatacaa tcgattcaca ccaaaaccat atacaagttc tgcccgacca
     5881 tttgaacgca agtttgaaag tcctaaattc aatcacaatc ttctgccaag tgaaactgca
     5941 cataaacctg acttgtcttc aaaaactccc acttctccaa aaactcttgt gaaatcgcac
     6001 agtttggcac agcctcctga gtttgacagt ggagttgaaa ctttctctat ccatgcagag
     6061 aagcctaaat atcaaataaa taatatcagc acagtgccta aagctattcc tgtgagtcct
     6121 tcagctgtgg aagaggatga agatgaagat ggtcatactg tggtggccac agcccgaggc
     6181 atatttaaca gcaatggggg cgtgctgagt tccatagaaa ctggtgttag tataattatc
     6241 cctcaaggag ccattcccga aggagttgag caggaaatct atttcaaggt ctgccgggac
     6301 aacagcatcc ttccaccttt agataaagag aaaggtgaaa cactgctgag tcctttggtg
     6361 atgtgtggtc cccatggcct caagttcctg aagcctgtgg agctgcgctt accacactgt
     6421 gatcctaaaa cctggcaaaa caagtgtctt cccggagatc caaattatct cgttggagca
     6481 aactgtgttt ctgtccttat tgaccacttt taaggatcca ccggatctag ataactgatc
     6541 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     6601 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     6661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     6781 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     6841 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     6901 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     6961 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7021 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7081 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7141 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7201 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     7261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7321 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     7381 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7441 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     7501 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     7561 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     7621 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7681 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     7741 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7801 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7861 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7921 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7981 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     8041 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     8101 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     8161 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     8221 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     8281 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     8341 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8401 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     8461 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     8521 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     8581 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     8641 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     8701 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     8761 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     8821 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     8881 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     8941 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     9001 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9061 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9841 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     9901 atatatggag ttccg
//
