LOCUS       sequence-475630-        9762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NUP98::RAP1GDS1 in bicistronic retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Michmerhuizen NL, Heikamp EB, Iacobucci I, Umeda M, Arthur B, Mishra
            V, Park CS, Di Giacomo D, Hiltenbrand R, Gao Q, Radko-Juettner S, 
            Lott J, Martucci C, Subramanyam V, Hatton C, Wenge DV, Baviskar P, 
            Portola P, Claquin A, Chandra B, Baggett DW, Khalighifar A, Huang H,
            Zhou P, Long L, Shi H, Sun Y, Papachristou EK, Sekhar Reddy 
            Chilamakuri C, de Luna Vitorino FN, Gongora JM, Wu H, Pounds SB, 
            Janke LJ, Kentsis A, D'Santos CS, Garcia BA, Kriwacki RW, Chi H, 
            Klco JM, Armstrong SA, Mullighan CG
  TITLE     KAT6A and KAT7 Histone Acetyltransferase Complexes Are Molecular 
            Dependencies and Therapeutic Targets in NUP98-Rearranged Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772.
  PUBMED    40536430
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2025 Jun 19. doi: 10.1158/2159-8290.CD-24-1772."
COMMENT     Sequence Label: CL20_MSCV-NUP98::RAP1GDS1-ires-GFP
FEATURES             Location/Qualifiers
     source          1..9762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..40
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3302..3326)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3353..3769)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(3369..3385)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3401..3423)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3836..4177
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             4241..4757
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(4957..4976)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5104..5126)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5320..5339)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5439..5461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5499..5517)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5585..5689
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5690..5758,5759..6550)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5908..5927)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6721..7309
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7210..7229
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7463..7480
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7597..7618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7633..7638,7639..7656,7657..7663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7671..7687
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             7849..8363
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(8465..9178,9179..9181,9182..9184))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8510..8531)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8859..8878
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9118..9139
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    9196..9748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9358..9377)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     9587..9604
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ggaattcgtt atcgaaccac tttgtacaag aaagctgggt tgcggccgca ctcgagctag
       61 ctttccacag taagtctctg ctctgtgaga gaggcctgct gggcaacact tttgttctca
      121 tgactgcgaa gtttggatac gacatctaga aaagccaaat cctgtacttc cttgtgtaga
      181 cattcagatc ccataagagc acatatcagg accatggaat tatatttgat ttcaggagca
      241 cttctctcat ctgctagcag tctatgtaaa atctgtacaa gtttagcact ttctagatct
      301 ttctcagcag tgcccaattc taaagctgct attaatgcca aagcaacaag agcttcattc
      361 tgcattatta catgttcact agttgccatg gtaactagat gcttgatgcc accactctgc
      421 acaatggttt taattacatc ttttgattta ctgtgtcgta taagggcaga cagcagtctg
      481 tttgactccc ccatcacacc agcatgatct ttggcttcac accattccac caaacgctcc
      541 actaacttaa cattctttcc caattgttca gcagcttctg cttgtgcatc tattaacatt
      601 cttaatgttc ccagaagttt gaactgaaca ggaggcattt cagatttaag aaatttcaaa
      661 actgcctctg tgaccccagc tgataacatc tttgctttat ttataactgg aatggccagg
      721 tttctgaggg cacttagtgc tgcatgctgt actgttacat ttccatcttc tacatgtctg
      781 tccagtaaat ccataagttt ttctacaatc ccattgtcta ccatatgaat acaatttgca
      841 tcatttctgg caaaatttgc aattgccaat gctccagcaa gctgtagctg gtggttattt
      901 gatgggatcc aagagagtac cctttgaaat acactacctt ttcctccttc aaataacttc
      961 tgcatggatt catctccaag aagtaataaa accatgagat ctgaaccagt tttgagctca
     1021 gtaatatcat cttctttgtc actatccact ttttgctgaa caatctctag tagacactct
     1081 actaggcctg cttcaaccag ctgtagttta atagcatcat tttctgccaa tggagcaaga
     1141 acttcaaaaa tcatttctct cttatcatgt tctatttgtt tcttgaagag ttttactagc
     1201 tcttcagcaa tgtttgtact ggcaaactgt tctttacttg actcaagttc tgctaaatta
     1261 ccaaatgcaa caagacacat ttctgtaaga gctgcatttt ggcagtggat gcccagtaat
     1321 ttcactaagg taggaataac acccatattg ataagctgag cttgaagcga atcattctca
     1381 ttgctatagt tcatcagcat gccacaaaag acagtcaaga gcttctcatt ggcgggatct
     1441 gttatactgc acagtgacct taaatggtca attacaatct gtgcaccacc tgcttggtca
     1501 actgcacttc tgccctcatg gctatcgtaa catatgtttc ctagagccct gcccgtttga
     1561 agcagcactt cctggtcttt gctatttagc agctgcacca gtggtgaaat caatccagca
     1621 tccacacatg gaattcgcat aaactcattt ttggctactt ctgctatgat gttagctact
     1681 ttggctttgc aggaagactg tggagtcaac agacttgcaa acagctgaag tattccactt
     1741 gcttggattt tttcacttgt ttccgtatta ttttgagcca gggcttgaag cagacaatcc
     1801 aagcatcctt ctaaactatc ctcagtcttg tcaacagctg ttatcttcag cttcttcaag
     1861 gtatcactga gattatctac tggggcctgg ggggctccaa agcccaaagt ggctgtcgta
     1921 gtattaaatc caggggcccc aaaggctcct gtaccaagag gccctccaat cttaggttgg
     1981 ttgttcccaa acaaagatgc ctgtccagca ccaagagctg ttccaaatcc tgcaccaagc
     2041 ccagttccaa gagtcccagg tgctggttta cttccaaaaa tactattccc actggtattt
     2101 gtgccaaaac caaagagccc gccactggtt gtaccaaatg aaggtgcact ggttgtgctg
     2161 cttccaaatg tagtaagctt gttattgcca aacagggtcg aaccaacagc accaaatcca
     2221 gtattggtct gcccaaagag acctgttcct gttccaaatg ctgtcccagt gctggtgttt
     2281 gtagctgtcc caaaaagacc tccaggctgt gaggcttggg ttactccaaa taatcccatg
     2341 gtgttggtgc ttggctgtcc tatggtgctg gtattaccaa aggaaaagcc agtgttctgg
     2401 gtggttgtag cctggccaaa tggtttgctg aagaggctgg tagtctgctg attctgttgg
     2461 ccaaagagac cacctggatt tgttccaaat ccagttgtac tagttccaaa ggcagttttg
     2521 ttctgaccat atgcaaagcc tgaattagtg gtggaggagc tgaagagtcc tgttgcgctg
     2581 gaagtggctg gagaagaccc aaacaagcca gttgtggtac ctgctcccac ctggttctgt
     2641 gggcccttcc tgttagcctg ataatcctct aaacgaagtt cctctagtga cttgctttca
     2701 tattctttca tagcagtaat acactggtgc ttggtactta tgttagtgct aactccagct
     2761 ttgaccatag tatctgtacc agttggaggg ttaaatttaa tagtagtccc agtaggagca
     2821 gctgtaaaac tacttggccc aaagagggag ccagatgtgc tgccaaaagg attagaggtg
     2881 gtatttgtgg ttccaaagag tcctccactg ctagtactgg ttccaaaatt gccaaagcca
     2941 gttggtttat tttgtgcaaa ggcattgttt tgggatgaga agagactggt ccctgtgctt
     3001 gcagttccaa acaaggtatt tgctgttcct gttgacgtac caaacccaaa gccagtgctt
     3061 gtggaggtag ctggctggct aaatgaactg gttccaaaca atcctcctgg tttagtctgt
     3121 gaatttccaa agaggcctcc agtattgttg ctagaaccaa atgcagatgt tccaaatgcc
     3181 cctccactag tagtgccaaa gccagtattc tgtccaaatg ttgaagttgt gccaaagcca
     3241 cctgtgccac ccccaaaggg tgttccaaat gatttgttaa acatggtacc gaattccttc
     3301 aagcctgctt ttttgtacaa acttgtttga taacctcgag agatctaatt ccggcgccta
     3361 gagaaggagt gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa
     3421 gggggagaga cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct
     3481 tgacccaggg aggggggtca aaagccaagg cttcccaggt cacgatgtag gggacctggt
     3541 ctgggtgtcc atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc
     3601 ggttaaaggt gccgtctcgc ggccatccga cgttaaaggt tggccattct gcagagcaga
     3661 aggtaaccca acgtctcttc ttgacatcta ccgactggtt gtgagcgatc cgctcgacat
     3721 ctttccagtg acctaaggtc aaacttaagg gagtggtaac agtctggccc taattttcag
     3781 acaaatacag aaacacagtc agacagagac aacacagaac gatgctgcag cgctgcagca
     3841 gacaagacgc gcggcttcgg ttccaaaccg aaagcaaaaa ttcagacgga ggcgggaact
     3901 gttttaggtt ctcgtctcct accagaacca catatcctga cggggtcgga ttccacatcg
     3961 actcccttcc tcaggtcggg ccacaaaaac ggcccccaaa gtccctggga cgtctcccag
     4021 ggttgcggcc gggtgttcag aactcgtcag ttccaccacg ggtccgccag atacagagct
     4081 agttagctaa ctagtacaga cgcaggcgca aacattagat gccggcacaa acacgcacaa
     4141 agacagagac agacacgaaa cgaccgctgg ccagcttacc tcccggcggg ggggtcggtg
     4201 gtccctgggc aggggtctcc aaatctcggt ggaacctcca aatgaaagac ccccgaggtg
     4261 ggcagtcaat caatctgagg agaccctccc aaggatcagc gagtccacga ttcggatgca
     4321 aacagcaaga ggctttattg ggaatacggg tacccgggcg acgcagtcta tcggaggact
     4381 ggcgcgccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
     4441 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
     4501 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc gggaccgcat
     4561 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
     4621 gtttggccca tattctgctg tctctctgtt cctaaccttg atctgaactt ctctattctc
     4681 agttatgtat tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac
     4741 aggtggggtc tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatct
     4801 atggctcgta ctctataggc ttcagctggt gatattgttg agtcaaaact agagcctgga
     4861 ccactgatat cctgtcttta acaaattgga ctaatcgcct ctacgccgga cgcatcgtgg
     4921 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     4981 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     5041 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgc
     5101 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5161 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5221 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
     5281 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5341 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5401 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5461 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     5521 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7381 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7441 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7501 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7561 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7621 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7681 ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa ctatttcagg
     7741 agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac ttaatattcg
     7801 actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat gaaagacccc
     7861 cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt
     7921 cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgacg cagtctatcg
     7981 gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag
     8041 cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt
     8101 tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggcggg
     8161 accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag
     8221 atatcctgtt tggcccatat tctgctgtct ctctgttcct aaccttgatc tgaacttctc
     8281 tattctcagt tatgtatttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca
     8341 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
     8401 tttatcgata agcttggctg caggtcgacg gtatcgataa gcttgataat tcttaattaa
     8461 ttcattactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     8521 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     8581 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     8641 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcgccttga
     8701 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     8761 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     8821 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     8881 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     8941 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     9001 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     9061 tgccgtaggt ggcatcgccc tcgccctcgc cagacacgct gaacttgtgg ccgtttacgt
     9121 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     9181 ccatggttgt ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc
     9241 ggggggccta gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc
     9301 cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat
     9361 acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg
     9421 ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg
     9481 tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa
     9541 gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg
     9601 agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc
     9661 acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct
     9721 tattccaagc ggcttcggcc agtaacgtta gggggggggg gc
//
