LOCUS       sequence-475632-        3183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal centromeric shuttle vector depleted of restriction sites 
            outside the polylinker region, yeast selection marker TRP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Scutteri L, Barth P, Rahi SJ
  TITLE     Minimal shuttle vectors for Saccharomyces cerevisiae.
  JOURNAL   Synth Biol (Oxf). 2025 May 21;10(1):ysaf010. doi: 
            10.1093/synbio/ysaf010. eCollection 2025.
  PUBMED    40612073
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2025-05-21"; volume: "10(1):ysaf010. doi"; pages: " 
            10.1093/synbio/ysaf010. eCollection 2025"
COMMENT     Sequence Label: pLS414
FEATURES             Location/Qualifiers
     source          1..3183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..64
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      948..1536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1437..1456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1537..2040
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     2041..2057
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2041..2057
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2114..2131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2114..2130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2131..2408
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             2409..3083
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
ORIGIN
        1 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
       61 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgcagaag atcagttggg
      181 tgcgcgagtg ggttacatcg aactggacct caacagtggt aagatactag agagttttcg
      241 ccccgaagaa cgattcccaa tgatgtccac ttttaaggtt ctgctatgtg gcgcggtatt
      301 atcccgtatt gacgcggggc aagagcaact aggtcgccgc atacactatt ctcagaatga
      361 cttggttgag tattcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      421 attatgtagt gctgccataa cgatgagtga taacacggcg gcgaacttac ttctgacaac
      481 gattggagga ccaaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      541 ccttgatcgt tgggaaccag agctgaatga agccatacca aacgacgagc gtgacaccac
      601 gatgcctgtt gctatggcaa caaccttgcg gaaactatta actggcgaac tacttactct
      661 agcttcccgg caacagttaa tagactggat ggaggctgat aaagttgcag gaccacttct
      721 gcgctctgcg cttccggctg gctggtttat tgctgataaa tctggggccg gagagcgtgg
      781 gtcacgcggt atcatagcag cactagggcc agatggtaag ccctcccgta tcgtagttat
      841 ctacacgacg gggagccagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      901 tgcgtcactg attaagcatt ggtagtagaa aagatcaaag gatcttcttg agatcctttt
      961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1081 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1501 ttgtgatgct cgtcaggggg gcggagccta tggaaaatca cgtgctataa aaataattat
     1561 aatttaaatt ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa
     1621 agaaaaaata gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat
     1681 actacctttt atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt
     1741 ctgtgtagaa gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga
     1801 atgtatatct atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt
     1861 tgttgaaatt ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct
     1921 tctttattta ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa
     1981 ttcccaaatt attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc
     2041 caggaaacag ctatgacgcg cgcgagctcg gtacccggga tcctctagag tcgacctgca
     2101 ggcatgccct aggactggcc gtcgttttac aacgacatta ctatatatat aatataggaa
     2161 gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg ccagatggca
     2221 gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg
     2281 gagcttttct ttttttgccg attaagaatt cggtcgaaaa aagaaaagga gagggccaag
     2341 agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac acaaaggcag
     2401 cttggagtat gtctgttatc aatttcacag gtagttctgg tccattggtg aaagtttgcg
     2461 gcttgcagag cacagaggcc gccgaatgtg ctcttgattc cgatgctgac ttgctcggta
     2521 ttatatgtgt ccccaataga aagagaacta ttgacccggt tattgcaagg aaaatttcaa
     2581 gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt ggcgtgtttc
     2641 gtaatcaacc aaaggaggat gttttggctc tggtcaatga ttacggcatt gatatagtgc
     2701 aactgcatgg tgacgagtcg tggcaagaat accaagagtt cctcggtttg ccagttatta
     2761 aaagactcgt atttccaaaa gactgcaaca tactactcag cgcagcttca cagaaacctc
     2821 attcgtttat tcccttgttt gattcagaag cgggtgggac aggtgaactt ttggattgga
     2881 actcgatttc tgattgggta ggaaggcaag agagtcccga aagtttacat tttatgttag
     2941 ctggtggact gacgccagaa aatgttggtg atgcgcttag attaaatggc gttattggtg
     3001 ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa atagcaaatt
     3061 tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta tttatttaag tattgtttgt
     3121 gcatttgcct gcaagccttt tgaaaagcaa gcataaaata tctaaacata aaatctgtaa
     3181 aat
//
