LOCUS       sequence-475635-         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual sgRNA empty vector expressing BFP with Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM,
            Sekine S, Li Y, Ward ME, Narendra DP
  TITLE     A unified mechanism for mitochondrial damage sensing in 
            PINK1-Parkin-mediated mitophagy.
  JOURNAL   EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z.
  PUBMED    41266657
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Nov 20. doi: 10.1038/s44318-025-00604-z."
COMMENT     Sequence Label: pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(220..253)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     257..277
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tggttagtac cgggcccgct ctagagatcc gacgcgccat ctctaggccc gcgccggccc
       61 cctcgcacgg acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata
      121 atatttccta gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt
      181 taatactagc tacattttac atgataggct tggatttcta taacttcgta tagcatacat
      241 tatacgaagt tataaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt
      301 tttgagacta taagtatccc ttggagaacc accttgttga gacgggagtc gtcttcgctt
      361 tccagatctg ataacttgtg aagacgacca tcgtctcgtt taagagctaa gctggaaaca
      421 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      481 tgcttttttt ctcgagtact aggatccatt aggcg
//
