LOCUS       sequence-475637-         500 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Thayer JA, Petersen JD, Huang X, Gruel Budet LM, Hawrot J, Ramos DM,
            Sekine S, Li Y, Ward ME, Narendra DP
  TITLE     A unified mechanism for mitochondrial damage sensing in 
            PINK1-Parkin-mediated mitophagy.
  JOURNAL   EMBO J. 2025 Nov 20. doi: 10.1038/s44318-025-00604-z.
  PUBMED    41266657
REFERENCE   2  (bases 1 to 500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2025 Nov 20. doi: 10.1038/s44318-025-00604-z."
COMMENT     Sequence Label: pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2
FEATURES             Location/Qualifiers
     source          1..500
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     194..214
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     365..384
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tataagtatc ccttggagaa ccaccttgtt ggttgaggag taacggccca ggtttcagag
       61 ctaagcacaa gagtgcatag caagttgaaa taaggctagt ccgtttacaa cttgaaaaag
      121 tggcacccga gtcgggtgct ttttttgctc gaatctacac tcagctatgg cgcgccccaa
      181 ggtcgggcag gaagagggcc tatttcccat gattccttca tatttgcata tacgatacaa
      241 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
      301 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      361 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      421 cttgtggaaa gccagaaaca tgggaaggtc agcgtgtgaa gtgtttaaga gctaagctgg
      481 aaacagcata gcaagtttaa
//
