LOCUS       sequence-475644-       10187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned 
            in the destination vector pHW1133 to swap driver via RMCE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Khan H, Huang X, Raj V, Wang H
  TITLE     A versatile site-directed gene trap strategy to manipulate gene 
            activity and control gene expression in Caenorhabditis elegans.
  JOURNAL   PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 
            10.1371/journal.pgen.1011541. eCollection 2025 Jan.
  PUBMED    39841730
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2025-01-22"; volume: "21(1):e1011541. doi"; pages: "
            10.1371/journal.pgen.1011541. eCollection 2025 Jan"
COMMENT     Sequence Label: pHW1176
FEATURES             Location/Qualifiers
     source          1..10187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    38..54
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     43..65
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     62..78
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     97..117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        99..117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    185..218
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             259..282
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1437..1760
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(1778..1811)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1821..1850
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3667..3990)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5908..5932
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5935..6960
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5935..6960
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6961..6985)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7338..7371)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7386..7419)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(7437..7453)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7438..7454)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(7478..7497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7479..7497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7507..7524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7507..7523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7516..7538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7665..8120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7752..7771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7962..7983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8146..8250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8251..8319,8320..9111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8469..8488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9282..9870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9771..9790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10024..10041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10151..10172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10187,1..5,6..23,24..30)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
      121 caaaagctgg agctccaccg cggtggcggc cgctctagaa gggctgcagg aattcgatgc
      181 tagcgaagtt cctattctct agaaagtata ggaacttcta actaatctga tttaaatttt
      241 cagggtaccg gcggaagcga ctacaaggac gacgacgaca aggagccaac cacttgacta
      301 agtgataagc tagctaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa
      361 atctatgctt ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc
      421 tcttctcaat aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt
      481 gcgagtttat ataccttcca attttctttc tattgtattt tcaacttcta attttaattc
      541 agggaaactg cttcaacgca tcaaaaatga gtaaaggaga agaattgttc actggagttg
      601 tcccaatcct cgtcgagctc gacggagacg tcaacggaca caagttctcc gtctccggag
      661 agggagaggg agacgccacc tacggaaagc tcaccctcaa gttcatctgc accaccggaa
      721 agctcccagt cccatggcca accctcgtca ccaccttctg ctacggagtc caatgcttct
      781 cccgttaccc agaccacatg aagcgtcacg acttcttcaa gtccgccatg ccagagggat
      841 acgtccaaga gcgtaccatc ttcttcaagg taagtttaaa catatatata ctaactactg
      901 attatttaaa ttttcaggac gacggaaact acaagacccg tgccgaggtc aagttcgagg
      961 gagacaccct cgtcaaccgt atcgagctca aggtaagttt aaacagttcg gtactaacta
     1021 accatacata tttaaatttt cagggaatcg acttcaagga ggacggaaac atcctcggac
     1081 acaagctcga gtacaactac aactcccaca acgtctacat catggccgac aagcaaaaga
     1141 acggaatcaa ggtcaacttc aaggtaagtt taaacatgat tttactaact aactaatctg
     1201 atttaaattt tcagatccgt cacaacatcg aggacggatc cgtccaactc gccgaccact
     1261 accaacaaaa caccccaatc ggagacggac cagtcctcct cccagacaac cactacctct
     1321 ccacccaatc cgccctctcc aaggacccaa acgagaagcg tgaccacatg gtcctcctcg
     1381 agttcgtcac cgccgccgga atcacccacg gaatggacga gctctacaag taataaatgc
     1441 aaaatccttt caagcattcc cttcttctct atcactcttc tttctttttg tcaaaaaatt
     1501 ctctcgctaa tttatttgct tttttaatgt tattatttta tgacttttta tagtcactga
     1561 aaagtttgca tctgagtgaa gtgaatgcta tcaaaatgtg attctgtctg atgtactttc
     1621 acaatctctc ttcaattcca ttttgaagtg ctttaaaccc gaaaggttga gaaaaatgcg
     1681 agcgctcaaa tatttgtatt gtgttcgttg agtgacccaa caaaaagagg aaactttatt
     1741 gtgccgccaa gaaaaaagtc tcaaaggtaa gtttaaaata acttcgtata gcatacatta
     1801 tacgaagtta ttttcaggga gagcaaaagc ttatctccga ggaggacctc taaggatgat
     1861 cgacgccaac gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat
     1921 tatttattta ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa
     1981 tctaaacatt tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa
     2041 cacaatgtgc aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg
     2101 gtggaagggg gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa
     2161 caacattcaa ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg
     2221 aggaaggaaa ctagctgccc tcgattgtct atcttccatt ctcaccaagt ttcaacaact
     2281 ttattcagtc attcttctct ggttcatttc ccaaccaatc ttcccgaata aagcccatct
     2341 gtcctttcgg ccacattcct cacctacatc cgtactcctt atctcccgtt cttatccagt
     2401 cttttatcgt ctctgcgtct cgcacactgt ctcgtcggta atgtaaaaaa aaaacaaaaa
     2461 aaacttgaaa acttgcggaa agatttccta ataaaaggag acgaatccgg taggaaatcg
     2521 acaagtctct gcttctcgaa gactaggtga gttacatttc tttggccgaa ttattttgaa
     2581 atcctaaatt tgataatttc agagatgtct gtaaaacttg cgtgttatgt gacggcttcg
     2641 gtcactgtcg ccactcttat ggtatgcttc atgaccatgt caaccatcta ctcggaggtt
     2701 gatggattca gagagaagct tgataccgag atgaatgtgt tcagagtgag ttaaattata
     2761 ttttgaattt taaataattt ttaattttct agcaatctac caatggattg tggaaggaca
     2821 tagttgtcat cggaagatct agcaagcgtg tccgttgtca atatgaagag accaacgcta
     2881 ccccaactcc acatgctgat ggatccccat ctgctccacc aggtcaacca ccagcagttc
     2941 caccagtctt caaccagcca aagactccaa atggagccaa tggaaatgga ccaacctgca
     3001 actgcaatgc tgataacaag tgcccagctg gaccatccgg accaaaggga gttccaggag
     3061 ttccaggact cgacggagtt ccaggacttg acggtgttcc aggagttgga gctgatgata
     3121 tcgctccaca acgcgagtct gtcggatgct tcacttgccc acaaggacca gttggaccac
     3181 caggagctct tggaagacca ggaccacgtg gacttccagg accaagagga caaaatggaa
     3241 acccaggaag agatggacaa ccaggacatc caggagagca aggatcatcc ggtcaaatcg
     3301 gaaagatcgg agagccagga ccaccaggag agaagggacg cgacgccgag catccaatcg
     3361 gaagaccagg accaaaggga ccaagaggag atcaaggacc aacaggacca gctggacaga
     3421 acggtcttca cggaccacca ggagagccag gaaccgttgg accagaagga ccatctggaa
     3481 agcaaggacg tcaaggacca gacggaaccc agggagagac tggaccagac ggaagaccag
     3541 gaaaggatgc cgagtactgc cagtgcccag acaagtctcc accatcagag gctgtcaacg
     3601 ccaaccgtgg atacagaaat atctaaattg ttggtgtttt ctaataaaat atttgagatt
     3661 cagtgagact tttttcttgg cggcacaata aagtttcctc tttttgttgg gtcactcaac
     3721 gaacacaata caaatatttg agcgctcgca tttttctcaa cctttcgggt ttaaagcact
     3781 tcaaaatgga attgaagaga gattgtgaaa gtacatcaga cagaatcaca ttttgatagc
     3841 attcacttca ctcagatgca aacttttcag tgactataaa aagtcataaa ataataacat
     3901 taaaaaagca aataaattag cgagagaatt ttttgacaaa aagaaagaag agtgatagag
     3961 aagaagggaa tgcttgaaag gattttgcat ttatcttaat caccgtcttc gagaagacga
     4021 accatggcgc cagtttcaga atccaagttt ctaatatagt tcatcacaat gttgacgttg
     4081 gtccaacctc cggcttgcat aatctctggg atggaaactc cagcgcgagc catgtcacgg
     4141 gcagctccga cacgggcgga atgtccagac caagccaagt aacgttgtcc ggaatcatcc
     4201 ttggctccgt agatgagacg gtgggtagct tcgaagattc ctgaaaattt attttcagct
     4261 tattcttagc taatatgttg tttaaactta ccttcaagag ctcgagtcga gagttgtgat
     4321 gttgcactcg gggcagcaac tccgttcttg cgaacacgac agaacaggta gttgtttgga
     4381 tcatcggcga ctccggaaac cgaaatccag cgctcaacga gcttggtaac tccaagggag
     4441 agcgcttttt cgactccggc agtcgagacc agcgtttttg tacgtccgat gtggatgagc
     4501 attcttcctc cgtctgtgcg actaatatcc ttgacgcgaa tgcgggcaat ttcggcgata
     4561 cggagaagtg tgttgtaagc aattccgagg aaggcgaggt ttcggatgtc ctggcaacga
     4621 tcggaatttt ccataagaga acggacctga tcgaagtcgg ttctgaaaat ttcgtcaagt
     4681 cttaggattg aatacattaa tatttaaact tacctttcga aggcgagggc ttgcttggct
     4741 ctctctccag catcaacatt ttccttgcgg atacgtctca ttacgagaga tacggcatta
     4801 gaatctgatg ggcgtggaag tccagatcgg cgatggagca tattgagctg tccgagatgt
     4861 tgttggatcg tcttcaccgc caatccacga gcttggaggt agagcaagta gtcacgaacg
     4921 tcttctggct cggctgggaa ccacttgcgg ttgttaagct tgcaccaggc agcccaagaa
     4981 cggcacactg agagaagcat cttccaggtg tgctcggaga aggcttgtct gtcacggaac
     5041 atatccatga ggttcttgcg aacttcatca cttgtcgcgt caactggaag tgctggaaga
     5101 ttttgatgaa cagtgagaag atttgacata ttttcgaagt tttttagatg cactagaaca
     5161 aagcgtgttg gcttcctctg agcccgcttt ccttatatac ccgcattctg cagccttaca
     5221 gaatgttcta gaaggtccta gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag
     5281 acgcagacgg aaaatgtatc tgggtctctt tattgtgtac actacttttc catgtaccga
     5341 atgtgagtcg ccctcctttt gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat
     5401 agtataaata ccgttgaaaa taaataccga acaacatttg ctctaattgt gaaattagaa
     5461 atcttcaaac tataatcatg tcactttacc actatttccg tccagctcaa cgttccgttt
     5521 ttggtgatca tttttgcttt cgtcgtaaat ctacacacgc gtctcttccg tgcgagagtc
     5581 caagccagca gccaaattcg ttgactgagt attcaacgtt tatacgttgt cggcaacgag
     5641 aaataggaaa atgcatcggg aaatgttctt ttttcgattt tttccaaggt tttgacaaat
     5701 tttaccacga attttgctat gttttcaatt aaaaaatatg ttattcaact gtttctatga
     5761 ggaaaataag gctttgcatg taattttctt attcagcata atttttaatt aatttgaatt
     5821 ttctgtccta acgtttattt tgttttcttg gttatgactg atctgaaatt aatttttgaa
     5881 ttttaaggta atatgtcagg cggtgccgca agtttgtaca aaaaagcagg ctccatgaaa
     5941 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     6001 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     6061 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     6121 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     6181 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     6241 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     6301 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     6361 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     6421 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     6481 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     6541 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     6601 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     6661 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     6721 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     6781 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     6841 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     6901 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     6961 acccagcttt cttgtacaaa gtgggtccaa ttactcttca acatccctac atgctctttc
     7021 tccctgtgct cccaccccct atttttgtta ttatcaaaaa acttctctta atttctttgt
     7081 tttttagctt cttttaagtc acctctaaca atgaaattgt gtagattcaa aaatagaatt
     7141 aattcgtaat aaaaagtcga aaaaaattgt gctccctccc cccattaata ataattctat
     7201 cccaaaatct acacaatgtt ctgtgtacac ttcttatgtt ttttacttct gataaatttt
     7261 tttgaaacat catagaaaaa accgcacaca aaatacctta tcatatgtta cgtttcagtt
     7321 tatgaccgca atttttaata acttcgtata gcatacatta tacgaagtta ttttcagggt
     7381 cccgggaagt tcctatacta tttgaagaat aggaacttcc ccgggatcaa gcttatcgat
     7441 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
     7501 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7561 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7621 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     7681 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     7741 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     7801 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     7861 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7921 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7981 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8041 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     8101 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8161 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8221 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8281 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8341 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8401 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8461 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8521 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8581 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8641 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8701 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8761 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8821 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8881 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8941 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9001 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9061 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     9961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10021 cgcagcgagt cagtgagcga ggaagcggcc caatacgcaa accgcctctc cccgcgcgtt
    10081 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10141 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggct
//
