LOCUS       sequence-475653-       11036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTNNA1 M1-domain deletion mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Wang Y, Yemelyanov A, Go CD, Kim SK, Quinn JM, Flozak AS, Le PM, 
            Liang S, Gingras AC, Ikura M, Ishiyama N, Gottardi CJ
  TITLE     alpha-Catenin force-sensitive binding and sequestration of LZTS2 
            leads to cytokinesis failure.
  JOURNAL   J Cell Biol. 2025 Mar 3;224(3):e202308124. doi: 
            10.1083/jcb.202308124. Epub 2025 Jan 9.
  PUBMED    39786338
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-03-3"; volume: "224(3):e202308124. doi"; pages: 
            " 10.1083/jcb.202308124. Epub 2025 Jan 9"
COMMENT     Sequence Label: pLV-mEGFP-hCTNNA1_DM1-domain
FEATURES             Location/Qualifiers
     source          1..11036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..290
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             318..917
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(318..337)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     814..834
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(918..938)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    951..1539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1004..1024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1422..1433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1627..1860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1932..2053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1969..1988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2023..2042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2093..2228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2248..2267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2249..2267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2277..2294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2277..2293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2286..2308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2435..2890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2522..2541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2732..2753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2916..3020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3021..3089,3090..3881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3239..3258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4052..4640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4541..4560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4794..4811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4928..4949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4964..4969,4970..4987,4988..4994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5002..5018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5007..5029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5026..5042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5026..5042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5061..5081
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5063..5081
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5109..5335
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5336..5516
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5563..5688
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6181..6414
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6599..6643
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6909..7026
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7035..7055
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        7116..7419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7420..7623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7574..7594
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7644..7668
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7676..7685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7682..7684,7685..7687,7688..8398)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7727..7748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7988..8007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8335..8356
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(10778..10802)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(10834..11036,1..9)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg ccgccctacc
       61 tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc
      121 tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg cctttgtccg
      181 gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct gaccctgctt
      241 gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc caagctgtga
      301 ccggcgccta cgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
      361 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
      421 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
      481 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
      541 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
      601 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
      661 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
      721 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
      781 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
      841 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
      901 gcaagcccgg tgcctgacaa ctttattata catagttgat caattccgat aatcaacctc
      961 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     1021 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     1081 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     1141 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     1201 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     1261 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     1321 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     1381 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     1441 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     1501 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgggaattc ccgcggttcg
     1561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     1621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     1681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     1741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     1801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     1861 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     1921 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2041 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     2101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2161 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2221 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     2281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2401 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     2461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2881 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3001 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3061 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3121 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3181 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3241 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3301 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3361 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3421 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3481 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3541 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3601 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3721 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3961 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4021 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4081 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4141 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4201 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4261 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4321 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4381 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4441 agctatgaga aagcgccacg cttcccgaag agagaaaggc ggacaggtat ccggtaagcg
     4501 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4561 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4621 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4681 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4741 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4801 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4921 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     4981 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5041 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     5101 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     5161 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     5221 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     5281 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     5341 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5401 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5461 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     5521 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     5581 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     5641 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     5701 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     5761 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     5821 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     5881 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     5941 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     6001 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     6061 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     6121 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     6181 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     6241 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     6301 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     6361 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     6421 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     6481 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     6541 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     6601 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     6661 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     6721 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     6781 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     6841 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     6901 tcgctagctt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     6961 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     7021 aattttacta gtatcaactt tgtatagaaa agttgtagtt attaatagta atcaattacg
     7081 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     7141 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7201 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7261 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     7321 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     7381 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7441 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7501 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     7561 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     7621 gctggtttag tgaaccgtca gatccaagtt tgtacaaaaa agcaggctct cgagtgccac
     7681 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     7741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     7801 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     7861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     7921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     7981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     8041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     8101 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     8161 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     8221 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     8281 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     8341 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg
     8401 cagcggcagc ggcagctcta gaatgactgc tgtccatgca ggcaacataa acttcaagtg
     8461 ggatcctaaa agtctagaga tcaggactct ggcagttgag agactgttgg agcctcttgt
     8521 tacacaggtt acaacccttg taaacaccaa tagtaaaggg ccctctaata agaagagagg
     8581 tcgttctaag aaggcccatg ttttggctgc atctgttgaa caagcaactg agaatttctt
     8641 ggagaagggg gataaaattg cgaaggagag ccagtttctc aaggaggagc ttgtggctgc
     8701 tgtagaagat gttcgaaaac aaggtgattt gatgaaggct gctgcaggag agttcgcaga
     8761 tgatccctgc tcttctgtga agcgaggcaa catggttcgg gcagctcgag ctttgctctc
     8821 tgctgttacc cggttgctga ttttggctga catggcagat gtctacaaat tacttgttca
     8881 gctgaaagtt gtggaagatg gtatcttgaa gttgaggaat gctggcaatg aacaagactt
     8941 aggaatccag tataaagccc taaaacctga agtggataag ctgaacatta tggcagccaa
     9001 aagacaacag gaattgaaag atgttggcca tcgtgatcag atggctgcag ctagaggaat
     9061 cctgcagaag aacgttccga tcctctatac tgcatcccag gcatgcctac agcaccctga
     9121 tgtcgcagcc tataaggcca acagggacct gatatacaag cagctgcagc aggcggtcac
     9181 aggcatttcc aatgcagccc aggccactgc ctcagacgat gcctcacagc accaggaaac
     9241 caatgttcca cttttggtat tgattgaagc tgcaaagaat ggaaatgaga aagaagttaa
     9301 ggagtatgcc caagttttcc gtgaacatgc caacaaattg attgaggttg ccaacttggc
     9361 ctgttccatc tcaaataatg aagaaggtgt aaagcttgtt cgaatgtctg caagccagtt
     9421 agaagccctc tgtcctcagg ttattaatgc tgcactggct ttagcagcaa aaccacagag
     9481 taaactggcc caagagaaca tggatctttt taaagaacaa tgggaaaaac aagtccgtgt
     9541 tctcacagat gctgtcgatg acattacttc cattgatgac ttcttggctg tctcagagaa
     9601 tcacattttg gaagatgtga acaaatgtgt cattgctctc caagagaagg atgtggatgg
     9661 cctggaccgc acagctggtg caattcgagg ccgggcagcc cgggtcattc acgtagtcac
     9721 ctcagagatg gacaactatg agccaggagt ctacacagag aaggttctgg aagccactaa
     9781 gctgctctcc aacacagtca tgccacgttt tactgagcaa gtagaagcag ccgtggaagc
     9841 cctcagctcg gaccctgccc agcccatgga tgagaatgag tttatcgatg cttcccgcct
     9901 ggtatatgat ggcatccggg acatcaggaa agcagtgctg atgataagga cccctgagga
     9961 gttggatgac tctgactttg agacagaaga ttttgatgtc agaagcagga cgagcgtcca
    10021 gacagaagac gatcagctga tagctggcca gagtgcccgg gcgatcatgg ctcagcttcc
    10081 ccaggagcaa aaagcgaaga ttgcggaaca ggtggccagc ttccaggaag aaaagagcaa
    10141 gctggatgct gaagtgtcca aatgggacga cagtggcaat gacatcattg tgctggccaa
    10201 gcagatgtgc atgattatga tggagatgac agactttacc cgaggtaaag gaccactcaa
    10261 aaatacatcg gatgtcatca gtgctgccaa gaaaattgct gaggcaggat ccaggatgga
    10321 caagcttggc cgcaccattg cagaccattg ccccgactcg gcttgcaagc aggacctgct
    10381 ggcctacctg caacgcatcg ccctctactg ccaccagctg aacatctgca gcaaggtcaa
    10441 ggccgaggtg cagaatctcg gcggggagct tgttgtctct ggggtggaca gcgccatgtc
    10501 cctgatccag gcagccaaga acttgatgaa tgctgtggtg cagacagtga aggcatccta
    10561 cgtcgcctct accaaatacc aaaagtcaca gggtatggct tccctcaacc ttcctgctgt
    10621 gtcatggaag atgaaggcac cagagaaaaa gccattggtg aagagagaga aacaggatga
    10681 gacacagacc aagattaaac gggcatctca gaagaagcac gtgaacccgg tgcaggccct
    10741 cagcgagttc aaagctatgg acagcatcta atctagaacc cagctttctt gtacaaagtg
    10801 gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
    10861 ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg
    10921 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
    10981 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgcc
//
