LOCUS       sequence-475654-       10697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTNNA1 M2,3-domain deletion mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Wang Y, Yemelyanov A, Go CD, Kim SK, Quinn JM, Flozak AS, Le PM, 
            Liang S, Gingras AC, Ikura M, Ishiyama N, Gottardi CJ
  TITLE     alpha-Catenin force-sensitive binding and sequestration of LZTS2 
            leads to cytokinesis failure.
  JOURNAL   J Cell Biol. 2025 Mar 3;224(3):e202308124. doi: 
            10.1083/jcb.202308124. Epub 2025 Jan 9.
  PUBMED    39786338
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-03-3"; volume: "224(3):e202308124. doi"; pages: 
            " 10.1083/jcb.202308124. Epub 2025 Jan 9"
COMMENT     Sequence Label: pLV-mEGFP-hCTNNA1_DM2,3-domain
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2010..2313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2314..2517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2468..2488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2538..2562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2570..2579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2576..2578,2579..2581,2582..3292)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2621..2642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2882..2901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3229..3250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5333..5357)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5389..5600
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5613..5881
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5909..6508
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5909..5928)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6405..6425
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6509..6529)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6542..7130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6595..6615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7013..7024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7218..7451
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7523..7644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7560..7579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7614..7633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7684..7819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7839..7858)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7840..7858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7868..7885)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7868..7884)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7877..7899)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8026..8481
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8113..8132)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8323..8344
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8507..8611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8612..8680,8681..9472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8830..8849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9643..10231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10132..10151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10385..10402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10519..10540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10555..10560,10561..10578,10579..10585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10593..10609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10598..10620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10617..10633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10617..10633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10652..10672
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10654..10672
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgt agttattaat agtaatcaat tacggggtca
     1981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2161 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2221 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2461 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     2521 ttagtgaacc gtcagatcca agtttgtaca aaaaagcagg ctctcgagtg ccaccatggt
     2581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3181 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg
     3301 cagcggcagc tctagaatga ctgctgtcca tgcaggcaac ataaacttca agtgggatcc
     3361 taaaagtcta gagatcagga ctctggcagt tgagagactg ttggagcctc ttgttacaca
     3421 ggttacaacc cttgtaaaca ccaatagtaa agggccctct aataagaaga gaggtcgttc
     3481 taagaaggcc catgttttgg ctgcatctgt tgaacaagca actgagaatt tcttggagaa
     3541 gggggataaa attgcgaagg agagccagtt tctcaaggag gagcttgtgg ctgctgtaga
     3601 agatgttcga aaacaaggtg atttgatgaa ggctgctgca ggagagttcg cagatgatcc
     3661 ctgctcttct gtgaagcgag gcaacatggt tcgggcagct cgagctttgc tctctgctgt
     3721 tacccggttg ctgattttgg ctgacatggc agatgtctac aaattacttg ttcagctgaa
     3781 agttgtggaa gatggtatct tgaagttgag gaatgctggc aatgaacaag acttaggaat
     3841 ccagtataaa gccctaaaac ctgaagtgga taagctgaac attatggcag ccaaaagaca
     3901 acaggaattg aaagatgttg gccatcgtga tcagatggct gcagctagag gaatcctgca
     3961 gaagaacgtt ccgatcctct atactgcatc ccaggcatgc ctacagcacc ctgatgtcgc
     4021 agcctataag gccaacaggg acctgatata caagcagctg cagcaggcgg tcacaggcat
     4081 ttccaatgca gcccaggcca ctgcctcaga cgatgcctca cagcaccagg gtggaggagg
     4141 aggagaactg gcatatgcac tcaataactt tgacaaacaa atcattgtgg accccttgag
     4201 cttcagcgag gagcgcttta ggccttccct ggaggagcgt ctggaaagca tcattagtgg
     4261 ggctgccttg atggccgact cgtcctgcac gcgtgatgac cgtcgtgagc gaattgtggc
     4321 agagtgtaat gctgtccgcc aggccctgca ggacctgctt tcggagtaca tgggcaatgc
     4381 tggacgtaaa gaaagaagtg atgcactcaa ttctgcaata gataaaatga ccaagaagac
     4441 cagggacttg cgtagacagc tccgcaaagc tgtcatggac cacgtttcag attctttcct
     4501 gaggacccct gaggagttgg atgactctga ctttgagaca gaagattttg atgtcagaag
     4561 caggacgagc gtccagacag aagacgatca gctgatagct ggccagagtg cccgggcgat
     4621 catggctcag cttccccagg agcaaaaagc gaagattgcg gaacaggtgg ccagcttcca
     4681 ggaagaaaag agcaagctgg atgctgaagt gtccaaatgg gacgacagtg gcaatgacat
     4741 cattgtgctg gccaagcaga tgtgcatgat tatgatggag atgacagact ttacccgagg
     4801 taaaggacca ctcaaaaata catcggatgt catcagtgct gccaagaaaa ttgctgaggc
     4861 aggatccagg atggacaagc ttggccgcac cattgcagac cattgccccg actcggcttg
     4921 caagcaggac ctgctggcct acctgcaacg catcgccctc tactgccacc agctgaacat
     4981 ctgcagcaag gtcaaggccg aggtgcagaa tctcggcggg gagcttgttg tctctggggt
     5041 ggacagcgcc atgtccctga tccaggcagc caagaacttg atgaatgctg tggtgcagac
     5101 agtgaaggca tcctacgtcg cctctaccaa ataccaaaag tcacagggta tggcttccct
     5161 caaccttcct gctgtgtcat ggaagatgaa ggcaccagag aaaaagccat tggtgaagag
     5221 agagaaacag gatgagacac agaccaagat taaacgggca tctcagaaga agcacgtgaa
     5281 cccggtgcag gccctcagcg agttcaaagc tatggacagc atctaatcta gaacccagct
     5341 ttcttgtaca aagtggaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     5401 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     5461 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     5521 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     5581 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     5641 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     5701 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     5761 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     5821 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     5881 ccaagctgtg accggcgcct acgccaccat gaccgagtac aagcccacgg tgcgcctcgc
     5941 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6001 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6061 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6121 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6181 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6241 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6301 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6361 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6421 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6481 gtgcatgacc cgcaagcccg gtgcctgaca actttattat acatagttga tcaattccga
     6541 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6601 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6661 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6721 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6781 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6841 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6901 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6961 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7021 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7081 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt
     7141 cccgcggttc gctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7201 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7261 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7441 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7501 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7621 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7681 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7741 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7801 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7861 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7921 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7981 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8041 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8101 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8161 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     8461 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9121 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9781 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10021 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gagagaaagg cggacaggta
    10081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10381 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    10441 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
    10501 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    10561 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    10621 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
    10681 gctggagctg caagctt
//
