LOCUS       sequence-475711-       11084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTNNA1 Nii-domain deletion mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Wang Y, Yemelyanov A, Go CD, Kim SK, Quinn JM, Flozak AS, Le PM, 
            Liang S, Gingras AC, Ikura M, Ishiyama N, Gottardi CJ
  TITLE     alpha-Catenin force-sensitive binding and sequestration of LZTS2 
            leads to cytokinesis failure.
  JOURNAL   J Cell Biol. 2025 Mar 3;224(3):e202308124. doi: 
            10.1083/jcb.202308124. Epub 2025 Jan 9.
  PUBMED    39786338
REFERENCE   2  (bases 1 to 11084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-03-3"; volume: "224(3):e202308124. doi"; pages: 
            " 10.1083/jcb.202308124. Epub 2025 Jan 9"
COMMENT     Sequence Label: pLV-mEGFP-hCTNNA1_DNii domain
FEATURES             Location/Qualifiers
     source          1..11084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2010..2313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2314..2517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2468..2488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2538..2562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2570..2579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2576..2578,2579..2581,2582..3292)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2621..2642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2882..2901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3229..3250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5720..5744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5776..5987
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6000..6268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6296..6895
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6296..6315)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6792..6812
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6896..6916)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6929..7517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6982..7002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7400..7411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7605..7838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7910..8031
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7947..7966)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8001..8020
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8071..8206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8226..8245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8227..8245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8255..8272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8255..8271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8264..8286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8413..8868
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8500..8519)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8710..8731
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8894..8998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8999..9067,9068..9859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9217..9236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10030..10618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10519..10538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10772..10789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10906..10927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10942..10947,10948..10965,10966..10972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10980..10996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10985..11007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11004..11020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11004..11020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11039..11059
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11041..11059
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgt agttattaat agtaatcaat tacggggtca
     1981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2161 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2221 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2461 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     2521 ttagtgaacc gtcagatcca agtttgtaca aaaaagcagg ctctcgagtg ccaccatggt
     2581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3181 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg
     3301 cagcggcagc tctagaatga ctgctgtcca tgcaggcaac ataaacttca agtgggatcc
     3361 taaaagtcta gagatcagga ctctggcagt tgagagactg ttggagcctc ttgttacaca
     3421 ggttacaacc cttgtaaaca ccaatagtaa agggccctct aataagaaga gaggtcgttc
     3481 taagaaggcc catgttttgg ctgcatctgt tgaacaagca actgagaatt tcttggagaa
     3541 gggggataaa attgcgaagg agagccagtt tctcaaggag gagcttgtgg ctgctgtaga
     3601 agatgttcga aaacaaggtg atttgatgaa ggctgctgca ggagagttcg cagatgatcc
     3661 ctgctcttct gtgaagcgag gcaacatggt tcgggcagct cgagctttgc tctctgctgt
     3721 tacccggttg ctgattttgg ctgacatggc agatgtctac aaattacttg ttcagctgaa
     3781 agttgtggaa gatggtatct tgaagttgag gaatgctggc gaactggcat atgcactcaa
     3841 taactttgac aaacaaatca ttgtggaccc cttgagcttc agcgaggagc gctttaggcc
     3901 ttccctggag gagcgtctgg aaagcatcat tagtggggct gccttgatgg ccgactcgtc
     3961 ctgcacgcgt gatgaccgtc gtgagcgaat tgtggcagag tgtaatgctg tccgccaggc
     4021 cctgcaggac ctgctttcgg agtacatggg caatgctgga cgtaaagaaa gaagtgatgc
     4081 actcaattct gcaatagata aaatgaccaa gaagaccagg gacttgcgta gacagctccg
     4141 caaagctgtc atggaccacg tttcagattc tttcctggaa accaatgttc cacttttggt
     4201 attgattgaa gctgcaaaga atggaaatga gaaagaagtt aaggagtatg cccaagtttt
     4261 ccgtgaacat gccaacaaat tgattgaggt tgccaacttg gcctgttcca tctcaaataa
     4321 tgaagaaggt gtaaagcttg ttcgaatgtc tgcaagccag ttagaagccc tctgtcctca
     4381 ggttattaat gctgcactgg ctttagcagc aaaaccacag agtaaactgg cccaagagaa
     4441 catggatctt tttaaagaac aatgggaaaa acaagtccgt gttctcacag atgctgtcga
     4501 tgacattact tccattgatg acttcttggc tgtctcagag aatcacattt tggaagatgt
     4561 gaacaaatgt gtcattgctc tccaagagaa ggatgtggat ggcctggacc gcacagctgg
     4621 tgcaattcga ggccgggcag cccgggtcat tcacgtagtc acctcagaga tggacaacta
     4681 tgagccagga gtctacacag agaaggttct ggaagccact aagctgctct ccaacacagt
     4741 catgccacgt tttactgagc aagtagaagc agccgtggaa gccctcagct cggaccctgc
     4801 ccagcccatg gatgagaatg agtttatcga tgcttcccgc ctggtatatg atggcatccg
     4861 ggacatcagg aaagcagtgc tgatgataag gacccctgag gagttggatg actctgactt
     4921 tgagacagaa gattttgatg tcagaagcag gacgagcgtc cagacagaag acgatcagct
     4981 gatagctggc cagagtgccc gggcgatcat ggctcagctt ccccaggagc aaaaagcgaa
     5041 gattgcggaa caggtggcca gcttccagga agaaaagagc aagctggatg ctgaagtgtc
     5101 caaatgggac gacagtggca atgacatcat tgtgctggcc aagcagatgt gcatgattat
     5161 gatggagatg acagacttta cccgaggtaa aggaccactc aaaaatacat cggatgtcat
     5221 cagtgctgcc aagaaaattg ctgaggcagg atccaggatg gacaagcttg gccgcaccat
     5281 tgcagaccat tgccccgact cggcttgcaa gcaggacctg ctggcctacc tgcaacgcat
     5341 cgccctctac tgccaccagc tgaacatctg cagcaaggtc aaggccgagg tgcagaatct
     5401 cggcggggag cttgttgtct ctggggtgga cagcgccatg tccctgatcc aggcagccaa
     5461 gaacttgatg aatgctgtgg tgcagacagt gaaggcatcc tacgtcgcct ctaccaaata
     5521 ccaaaagtca cagggtatgg cttccctcaa ccttcctgct gtgtcatgga agatgaaggc
     5581 accagagaaa aagccattgg tgaagagaga gaaacaggat gagacacaga ccaagattaa
     5641 acgggcatct cagaagaagc acgtgaaccc ggtgcaggcc ctcagcgagt tcaaagctat
     5701 ggacagcatc taatctagaa cccagctttc ttgtacaaag tggaaggatc tgcgatcgct
     5761 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     5821 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     5881 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     5941 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     6001 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     6061 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     6121 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     6181 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     6241 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ccaccatgac
     6301 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6361 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6421 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6481 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6541 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6601 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6661 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6721 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6781 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6841 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacaact
     6901 ttattataca tagttgatca attccgataa tcaacctctg gattacaaaa tttgtgaaag
     6961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7081 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7141 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7201 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7261 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7321 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7381 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7441 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7501 ttgggccgcc tccccgcatc gggaattccc gcggttcgct ttaagaccaa tgacttacaa
     7561 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7621 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7681 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7741 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7801 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7861 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
     7921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8041 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     8101 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8161 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
     8221 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     8281 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     8341 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     8401 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8461 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8521 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8581 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     8641 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     8701 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8761 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8821 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     8881 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8941 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     9001 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     9061 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     9121 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     9181 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9241 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     9301 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     9361 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     9421 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9481 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9541 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9601 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9661 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9721 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9781 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9841 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10141 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    10201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10441 tcccgaagag agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    10621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    10681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    10741 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    10801 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    10861 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    10921 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    10981 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
    11041 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gctt
//
