LOCUS       sequence-475750-       11408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CTNNA1 eye phenotype via forward genetic screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Wang Y, Yemelyanov A, Go CD, Kim SK, Quinn JM, Flozak AS, Le PM, 
            Liang S, Gingras AC, Ikura M, Ishiyama N, Gottardi CJ
  TITLE     alpha-Catenin force-sensitive binding and sequestration of LZTS2 
            leads to cytokinesis failure.
  JOURNAL   J Cell Biol. 2025 Mar 3;224(3):e202308124. doi: 
            10.1083/jcb.202308124. Epub 2025 Jan 9.
  PUBMED    39786338
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2025-03-3"; volume: "224(3):e202308124. doi"; pages: 
            " 10.1083/jcb.202308124. Epub 2025 Jan 9"
COMMENT     Sequence Label: pLV-mEGFP-hCTNNA1_L436P
FEATURES             Location/Qualifiers
     source          1..11408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2010..2313
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2314..2517
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2468..2488
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2538..2562
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2570..2579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2576..2578,2579..2581,2582..3292)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2621..2642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2882..2901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3229..3250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6044..6068)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6100..6311
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6324..6592
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6620..7219
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6620..6639)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7116..7136
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(7220..7240)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7253..7841
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7306..7326)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7724..7735)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7929..8162
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8234..8355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8271..8290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8325..8344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8395..8530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8550..8569)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8551..8569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8579..8596)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8579..8595)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8588..8610)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8737..9192
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8824..8843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9034..9055
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9218..9322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9323..9391,9392..10183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9541..9560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10354..10942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10843..10862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11096..11113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11230..11251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11266..11271,11272..11289,11290..11296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11304..11320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11309..11331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11328..11344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11328..11344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11363..11383
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11365..11383
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgt agttattaat agtaatcaat tacggggtca
     1981 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2041 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2101 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2161 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     2221 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     2281 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     2341 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2401 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     2461 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
     2521 ttagtgaacc gtcagatcca agtttgtaca aaaaagcagg ctctcgagtg ccaccatggt
     2581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     2641 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     2701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     2761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     2821 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     2881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     2941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3001 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     3061 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     3121 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3181 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3241 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcagcgg
     3301 cagcggcagc tctagaatga ctgctgtcca tgcaggcaac ataaacttca agtgggatcc
     3361 taaaagtcta gagatcagga ctctggcagt tgagagactg ttggagcctc ttgttacaca
     3421 ggttacaacc cttgtaaaca ccaatagtaa agggccctct aataagaaga gaggtcgttc
     3481 taagaaggcc catgttttgg ctgcatctgt tgaacaagca actgagaatt tcttggagaa
     3541 gggggataaa attgcgaagg agagccagtt tctcaaggag gagcttgtgg ctgctgtaga
     3601 agatgttcga aaacaaggtg atttgatgaa ggctgctgca ggagagttcg cagatgatcc
     3661 ctgctcttct gtgaagcgag gcaacatggt tcgggcagct cgagctttgc tctctgctgt
     3721 tacccggttg ctgattttgg ctgacatggc agatgtctac aaattacttg ttcagctgaa
     3781 agttgtggaa gatggtatct tgaagttgag gaatgctggc aatgaacaag acttaggaat
     3841 ccagtataaa gccctaaaac ctgaagtgga taagctgaac attatggcag ccaaaagaca
     3901 acaggaattg aaagatgttg gccatcgtga tcagatggct gcagctagag gaatcctgca
     3961 gaagaacgtt ccgatcctct atactgcatc ccaggcatgc ctacagcacc ctgatgtcgc
     4021 agcctataag gccaacaggg acctgatata caagcagctg cagcaggcgg tcacaggcat
     4081 ttccaatgca gcccaggcca ctgcctcaga cgatgcctca cagcaccagg gtggaggagg
     4141 aggagaactg gcatatgcac tcaataactt tgacaaacaa atcattgtgg accccttgag
     4201 cttcagcgag gagcgcttta ggccttccct ggaggagcgt ctggaaagca tcattagtgg
     4261 ggctgccttg atggccgact cgtcctgcac gcgtgatgac cgtcgtgagc gaattgtggc
     4321 agagtgtaat gctgtccgcc aggccctgca ggacctgctt tcggagtaca tgggcaatgc
     4381 tggacgtaaa gaaagaagtg atgcactcaa ttctgcaata gataaaatga ccaagaagac
     4441 cagggacttg cgtagacagc tccgcaaagc tgtcatggac cacgtttcag attctttcct
     4501 ggaaaccaat gttccacttt tggtattgat tgaagctgca aagaatggaa atgagaaaga
     4561 agttaaggag tatgcccaag ttttccgtga acatgccaac aaattgattg aggttgccaa
     4621 cccagcctgt tccatctcaa ataatgaaga aggtgtaaag cttgttcgaa tgtctgcaag
     4681 ccagttagaa gccctctgtc ctcaggttat taatgctgca ctggctttag cagcaaaacc
     4741 acagagtaaa ctggcccaag agaacatgga tctttttaaa gaacaatggg aaaaacaagt
     4801 ccgtgttctc acagatgctg tcgatgacat tacttccatt gatgacttct tggctgtctc
     4861 agagaatcac attttggaag atgtgaacaa atgtgtcatt gctctccaag agaaggatgt
     4921 ggatggcctg gaccgcacag ctggtgcaat tcgaggccgg gcagcccggg tcattcacgt
     4981 agtcacctca gagatggaca actatgagcc aggagtctac acagagaagg ttctggaagc
     5041 cactaagctg ctctccaaca cagtcatgcc acgttttact gagcaagtag aagcagccgt
     5101 ggaagccctc agctcggacc ctgcccagcc catggatgag aatgagttta tcgatgcttc
     5161 ccgcctggta tatgatggca tccgggacat caggaaagca gtgctgatga taaggacccc
     5221 tgaggagttg gatgactctg actttgagac agaagatttt gatgtcagaa gcaggacgag
     5281 cgtccagaca gaagacgatc agctgatagc tggccagagt gcccgggcga tcatggctca
     5341 gcttccccag gagcaaaaag cgaagattgc ggaacaggtg gccagcttcc aggaagaaaa
     5401 gagcaagctg gatgctgaag tgtccaaatg ggacgacagt ggcaatgaca tcattgtgct
     5461 ggccaagcag atgtgcatga ttatgatgga gatgacagac tttacccgag gtaaaggacc
     5521 actcaaaaat acatcggatg tcatcagtgc tgccaagaaa attgctgagg caggatccag
     5581 gatggacaag cttggccgca ccattgcaga ccattgcccc gactcggctt gcaagcagga
     5641 cctgctggcc tacctgcaac gcatcgccct ctactgccac cagctgaaca tctgcagcaa
     5701 ggtcaaggcc gaggtgcaga atctcggcgg ggagcttgtt gtctctgggg tggacagcgc
     5761 catgtccctg atccaggcag ccaagaactt gatgaatgct gtggtgcaga cagtgaaggc
     5821 atcctacgtc gcctctacca aataccaaaa gtcacagggt atggcttccc tcaaccttcc
     5881 tgctgtgtca tggaagatga aggcaccaga gaaaaagcca ttggtgaaga gagagaaaca
     5941 ggatgagaca cagaccaaga ttaaacgggc atctcagaag aagcacgtga acccggtgca
     6001 ggccctcagc gagttcaaag ctatggacag catctaatct agaacccagc tttcttgtac
     6061 aaagtggaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     6121 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     6181 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     6241 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     6301 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     6361 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     6421 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     6481 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     6541 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     6601 gaccggcgcc tacgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6661 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6721 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6781 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6841 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6901 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6961 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7021 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7081 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7141 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7201 ccgcaagccc ggtgcctgac aactttatta tacatagttg atcaattccg ataatcaacc
     7261 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7321 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7381 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7441 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7501 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7561 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7621 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7681 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7741 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7801 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat tcccgcggtt
     7861 cgctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7921 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7981 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8041 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8101 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8161 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8221 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8341 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8401 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8461 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8521 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     8581 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8641 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8701 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     8761 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8821 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8881 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8941 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     9001 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     9061 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     9121 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     9181 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     9241 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9301 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9361 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9421 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9481 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9541 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     9601 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     9661 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     9721 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     9781 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     9841 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     9901 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     9961 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    10021 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    10081 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    10141 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    10201 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10261 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10321 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10381 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10441 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10501 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10561 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10621 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10681 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10741 tgagctatga gaaagcgcca cgcttcccga agagagaaag gcggacaggt atccggtaag
    10801 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10861 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10921 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10981 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    11041 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    11101 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    11161 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
    11221 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    11281 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    11341 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
    11401 gcaagctt
//
