LOCUS       sequence-47576-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: Lyn-CFP-GAI(1-151)
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(95..97,98..100,101..811)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(140..161)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(401..420)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     748..769
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             836..853
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtgaat tccggccacc atgggatgta taaaatcaaa
       61 agggaaagac agcgcggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      121 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      181 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      241 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctggggcgt
      301 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      361 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      421 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      481 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acatcagcca
      541 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      601 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      661 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      721 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      781 gatcactctc ggcatggacg agctgtacaa gagcgcagga ggaatgaaga gagatcatca
      841 tcatcatcat catcaagata agaagactat gatga
//
