LOCUS       sequence-475761-       17466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one bimolecular fluorescence complementation (BiFC) 
            vector is used to study the self-interation of AtbZIP63 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17466)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 17466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTHV3-(AtbZIP63-nV+AtbZIP63-cV)
FEATURES             Location/Qualifiers
     source          1..17466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1153..1863)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1431..1450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2306..2651)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2681..2855
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2901..2922)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(3904..4249)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    4279..4453
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(4571..5083,5084..5086,5087..5089))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     4764..4783
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5023..5044
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(6106..6782)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(10552..10569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10552..10568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10561..10583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10771..10795
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12096..12725
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13154..14227
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14293..14487
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14723..14745)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14831..14971
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14886..14905
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14986..15003)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15157..15745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15237..15256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15832..16626)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    17051..17075
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    17343..17364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17379..17384,17385..17402,17403..17409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17417..17433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17422..17444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17441..17457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17441..17457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     1201 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     1261 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     1321 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     1381 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     1441 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     1501 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     1561 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     1621 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     1681 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     1741 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     1801 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     1861 catggatcca gaactcgtaa acttcgtaac cgccttagtc ccttcagaaa cagcatgttt
     1921 cgccaactct ccaggcaaca caagtctcac cgcagtctga atctccctag aagtaatcgt
     1981 cggcttcttg ttgtacctcg caagcttcga agactcacca gcaagtttct caaagatatc
     2041 attgatgaaa ctgttcatga ttcccatggc tttgctggag attccgatat ctggatgaac
     2101 ttgcttcaac accttgaaga tgtagatctt gtatgtctca acgttcttct ttgatctctt
     2161 cttcttcttg tctccggcgc cggctggttc cttcgggagt ttctttccgg cttttggttt
     2221 cttctctgcc gctgctgctg gttcggctgc cggagttttc tctgccggtt tcttctccgc
     2281 tggtttctta tctgccttcg ccatctgtcc tctccaaatg aaatgaactt ccttatatag
     2341 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     2401 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     2461 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     2521 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     2581 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     2641 gaaaagtctc aattgccctt tggtcttctg agactgtatc gatctggatt ttagtactgg
     2701 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     2761 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     2821 tatctgggaa ctactcacac attattatgg agaaattact tgtacagctc gtccatgccg
     2881 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2941 gggtccatga gaccctgatc cccaacgctt cgaatacgtt tctgcagatg ttccaagctc
     3001 tcaactctac gcatcgaagc tgttctgttc atcttgcacc ctatcaaggc cttgcctttg
     3061 ttccccgagc taatgatctc tggtgtagtc acttggctgc ttgtagtgtc tggagaattt
     3121 gatgtctctg aaggaagaga gacagttgaa acaatctgag gcatattgtg gaacattggg
     3181 ttaaagccag tgagtctctt cactgtctct tcagccattt tcacctttgc tcgtagtgtc
     3241 tcaatattgg cttttaaaac tctgttttct acagatgcat cattgaatgt ttgagttaca
     3301 tcagtgagac ccttcatgag ttttgaattc tctacacgaa gctgtgaaac ttgtgtctct
     3361 agctcactca agtgtgcttg ctttcttctt ctggaccgtc tagctgattc tctattagag
     3421 agcatccttt taacgcgttt aacattagta gggttcatat tagtttcacc atcagcttct
     3481 tcttcatcac cagagagctc agatccactt gttatagcac tgctcatcat tggtgtagct
     3541 ttggaagaag caagagaggc ttgttctgat tcattggctc caccattgtc agatctacca
     3601 gaagtatcct gaggtttgat gaaagttccc cttttcatgg cgacagcagc acaagcaaga
     3661 ttaagtttac tcttgagaaa tgctctgtat tcctctgaat ctacaggaac attaggagga
     3721 gaggagacgg aaacaccaca cgccgtcgta gattctccgt cgtctgcagc ggcggaggat
     3781 tcttgtatga aacgattgaa tgcccattcg gaagcactgc gattcaacga cgtcattcca
     3841 ttaaccgacc agtgatggtt accggagatt tcttcgtcgg agaaaacttt ttccatggta
     3901 ccctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     3961 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     4021 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     4081 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
     4141 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
     4201 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
     4261 gtcttctgag actgtatcga tctggatttt agtactggat tttggtttta ggaattagaa
     4321 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
     4381 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
     4441 tattatggag aaattagtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg
     4501 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4561 cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
     4621 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4681 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4741 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4801 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4861 cggggtagcg ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg
     4921 gcacgggcag cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat
     4981 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     5041 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatc tgatccccaa
     5101 cgcttcgaat acgtttctgc agatgttcca agctctcaac tctacgcatc gaagctgttc
     5161 tgttcatctt gcaccctatc aaggccttgc ctttgttccc cgagctaatg atctctggtg
     5221 tagtcacttg gctgcttgta gtgtctggag aatttgatgt ctctgaagga agagagacag
     5281 ttgaaacaat ctgaggcata ttgtggaaca ttgggttaaa gccagtgagt ctcttcactg
     5341 tctcttcagc cattttcacc tttgctcgta gtgtctcaat attggctttt aaaactctgt
     5401 tttctacaga tgcatcattg aatgtttgag ttacatcagt gagacccttc atgagttttg
     5461 aattctctac acgaagctgt gaaacttgtg tctctagctc actcaagtgt gcttgctttc
     5521 ttcttctgga ccgtctagct gattctctat tagagagcat ccttttaacg cgtttaacat
     5581 tagtagggtt catattagtt tcaccatcag cttcttcttc atcaccagag agctcagatc
     5641 cacttgttat agcactgctc atcattggtg tagctttgga agaagcaaga gaggcttgtt
     5701 ctgattcatt ggctccacca ttgtcagatc taccagaagt atcctgaggt ttgatgaaag
     5761 ttcccctttt catggcgaca gcagcacaag caagattaag tttactcttg agaaatgctc
     5821 tgtattcctc tgaatctaca ggaacattag gaggagagga gacggaaaca ccacacgccg
     5881 tcgtagattc tccgtcgtct gcagcggcgg aggattcttg tatgaaacga ttgaatgccc
     5941 attcggaagc actgcgattc aacgacgtca ttccattaac cgaccagtga tggttaccgg
     6001 agatttcttc gtcggagaaa actttttcca tggtctcgga tctcattgcc ccccggatct
     6061 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     6121 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     6181 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     6241 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     6301 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
     6361 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
     6421 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
     6481 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tcacatcaat
     6541 ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg
     6601 ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat
     6661 cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac
     6721 agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct
     6781 caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt
     6841 cgtgctccac catgttggca agctgctcta gctcacgttg acggaaactg cagaacgatc
     6901 tcagttaggt catgggaaag ctgatattca tccctctgag actctatata attaaatgcg
     6961 tttgggggat tagctaattg agaatccata tagagaaata tggccgcgca gcgacgccgg
     7021 ttgaagaata tgaacaagga ataagatgaa caaggaagaa gatgaacaag caataattta
     7081 attaattatt gttaataagc ataaattatg aaaataattt tgatggtaat gaaagtatta
     7141 taaataatat ttaggagaaa atattgttaa ggaaatatct ggttatgttt atttgttaag
     7201 gtatatgggt ttttatagaa acccaggagc agctatggta agaaacccag gactcaaaaa
     7261 atgcctctca acttctctca tatgttgcgg agtctggagt cccatttata ccatagaaag
     7321 cattgggggt atagcatggt aatttcacat cggactccat accgcggcca tctatataat
     7381 attactgatg gccgcgcgaa gtcccggtat gccttttcag ccgttggatc tggagccgta
     7441 cctttttcgc gttcccattt tgcccctgga gtcccgagct atttacgacc ccctgccacc
     7501 gagtcccctt cgtggagcgc tcttcgtcca acacgtgtcc caacttttgc gcatttttga
     7561 ctacctgtcg cgcacttgaa ttaaagttgc ccttttattt tcttccaatt attttgcggc
     7621 tgagtagtct agtggatccc ccggagctcc acttgggata ggttaagaaa atatgtctgg
     7681 cttgtaaacg aaaagaatcg gggtttctag gcatttttta aatagtgacc caggactcaa
     7741 aaactagctc tcaacttctc tcatatgttg tggagtctgg agtcctttat atatgtttac
     7801 gctatagaag gcatttgggg actctcccgg gcccaccata ggactccata ccgcggccat
     7861 ctatataata ttactgatgg ccgcgcggat tttggagccc ccttttgtcg ttagcgcgac
     7921 ctttaattca aattaaaggt tgcttttttc tatcgtccga tcattttgcg cctgactagc
     7981 ctagatatta gcaacaactt cgcggctaag ttgttgaccg tcaagtataa attaatgggc
     8041 ttatggccta atgggtatag agcaaaatgg catgcctgca gactagtgct ttactctgat
     8101 ccgcagatat ttcatcagga aagagctttg caagttcctt cattgtagca tcaataattt
     8161 ctgagtcact acatgcaatc cattcttctg ctggggcaaa aactaactcc aacatggatt
     8221 ggtttggatt gtaatattcc ttacatgtta cagacatgtc agcataaaca cttaaaagcg
     8281 ggcttctgct aaagagtaga tgatcatagg tgttcttcaa tttcctatca aaccagatgt
     8341 gaacgttgat aactggaact ccaactaatt tctctaattt cttgaagtat gaaatctctc
     8401 tccagtcttc aggcactagt gaattcgcta gcgttaactg gccataatcc tgtgtatgca
     8461 acgttgaaaa ttcggatcta tttttacgat tcgatcatga attaataaaa tttaaatttt
     8521 attgaatgat tttctagtac ataactgaca tacgacttat cagttgcgaa acgaacagct
     8581 ctaattacat tgttaagtga aattacgcct aattcgtcta agtacaacat aaccaactgt
     8641 ttaaacctaa ttaaataagt cgacccagaa gctgtcagag atgtcgtcca gacttggaag
     8701 ttcaggtaag ctttgtggag atgcaacgct ctccgtaagt tgtggttgaa tcggatctgg
     8761 acgtggtata tacgcgtcct ggtgtatagc agatcctcta ctccgtatat cttgaaatat
     8821 aggggattta ttatctccca gatatacacg ccattctctg cctgacgtgc agtgatgatt
     8881 tcccctgtgc gtgaatccat gaccggtgca gcctatgtgg aagtatatgg tgcacccaca
     8941 gtttagatca atgcgtcgtc gtctaatcgc cctcctcttg gcttgcctgt gtgccttctt
     9001 gatagagggg ggcgtcgaga gtgatgaaga taacattctt gagagtccaa tgtttgagcg
     9061 acgcattttc cgctttatcc aggaaatctt tatagctgga tccctcgcct ggattgcaaa
     9121 gcacgattga agggatccct cctttaattt gaactggttt cccgtacttg caatttgact
     9181 gccagtcttt ttgggcccca agaagctctt tccaatgctt tagctttagg tattgcggtg
     9241 ctacgtcatc aatgacgtta taggcaacat tattcgagta cactcgatta ttgaagtcca
     9301 ggtgtccact gaggtaatta tgtgggccta aagcacgagc ccacatcgtc ttgcccgttc
     9361 tagaatcacc ttccactatg agactaactg gtcttacagg ccgcgcagcg ggacccattc
     9421 caaaataatc atccgcccat tcttgcatct gttcaggaac gttagtgaaa gaggaaagtg
     9481 gaaatggagg gacccattgt tcaggcgcct ttttgaaaag gcgttccaag ttcgctgtaa
     9541 tgttatggta actcacgatg aacgcccttg gatctccggc ccggataatt gcaagagctt
     9601 ccgccaaact aggtgcattg atggcgttat aatagacgtc gtctttattc ttctttgtac
     9661 ccccaggcac tttgaactgt ccggattcac aacaatcgcc gtctttggtg atgtaattct
     9721 tgacggcgtt ggcgtctttg gctgcttgta tgtttgggtg aaaattggtc gaccttctgg
     9781 ggtgagtaag gtcgaaaaat ctcttattct tgatgttgga ctttccagag agttggatga
     9841 gacagtgaag gtgtgggaat ccgtccgagt gctcctctct ggcaactcga atatatgttg
     9901 gtttgacgac tgaccatgat aaggtttgaa gcatttgaag agcttcattt tttgggatat
     9961 cgcacttggg ataggttaag aaaatatgtc tggcttgtaa acgaaaagaa tcggggtttc
    10021 taggcatttt ttaaatagtg acccaggact caaaaactag ctctcaactt ctctcatatg
    10081 ttgtggagtc tggagtcctt tatatatgtt tacgctatag aaggcatttg gggactctcc
    10141 cgggcccacc ataggactcc ataccgcggc catctatata atattactga tggccgcgcg
    10201 gattttggag cccccttttg tcgttagcgc gacctttaat tcaaattaaa ggttgctttt
    10261 ttctatcgtc caatcatttt gcgcctgact agcctagata ttagcaacaa cttcgcgact
    10321 aagttgttga ccgtcaagta taaattaatg ggcttatggc ccaatgggta tagagcaaaa
    10381 tgcccaagcg tgatgccccg tggcgcttat tagcgggtac gtctaaggta agtcgaaatg
    10441 ccaactactc tcccttgaga gtattgggcc ctaaagttaa taagggacct cgaggggggg
    10501 cccggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt
    10561 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10621 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10681 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
    10741 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
    10801 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
    10861 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
    10921 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
    10981 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
    11041 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
    11101 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
    11161 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
    11221 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
    11281 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
    11341 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
    11401 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
    11461 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
    11521 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
    11581 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
    11641 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
    11701 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
    11761 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
    11821 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
    11881 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
    11941 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
    12001 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
    12061 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
    12121 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
    12181 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
    12241 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
    12301 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
    12361 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
    12421 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
    12481 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
    12541 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
    12601 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
    12661 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
    12721 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
    12781 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
    12841 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
    12901 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
    12961 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
    13021 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
    13081 ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc
    13141 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
    13201 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
    13261 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
    13321 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
    13381 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
    13441 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
    13501 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
    13561 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
    13621 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
    13681 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
    13741 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
    13801 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
    13861 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
    13921 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
    13981 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
    14041 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
    14101 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
    14161 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
    14221 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
    14281 aggtgtcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
    14341 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
    14401 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
    14461 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
    14521 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
    14581 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
    14641 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
    14701 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    14761 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    14821 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
    14881 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
    14941 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
    15001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    15061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    15121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    15181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    15241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    15301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    15361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    15421 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    15481 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    15541 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
    15601 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    15661 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    15721 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    15781 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
    15841 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    15901 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    15961 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    16021 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    16081 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    16141 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    16201 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    16261 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    16321 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    16381 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    16441 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    16501 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    16561 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    16621 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    16681 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    16741 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    16801 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    16861 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    16921 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    16981 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    17041 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
    17101 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg ggatagccct
    17161 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
    17221 catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    17281 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    17341 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    17401 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    17461 attacg
//
