LOCUS       sequence-475762-       16524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one bimolecular fluorescence complementation (BiFC) 
            vector is used to study the self-interation of AtbZIP63 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16524)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 16524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTHV3-(nV+AtbZIP63-cV)
FEATURES             Location/Qualifiers
     source          1..16524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1153..1863)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1431..1450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2306..2651)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2681..2855
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2901..2922)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(3904..4249)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    4279..4453
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(4571..5083,5084..5086,5087..5089))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     4764..4783
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5023..5044
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5164..5840)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9610..9627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9610..9626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9619..9641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9829..9853
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11154..11783
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12212..13285
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13351..13545
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13781..13803)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13889..14029
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13944..13963
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14044..14061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14215..14803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14295..14314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14890..15684)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16109..16133
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    16401..16422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16437..16442,16443..16460,16461..16467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16475..16491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16480..16502
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16499..16515
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16499..16515
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     1201 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     1261 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     1321 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     1381 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     1441 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     1501 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     1561 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     1621 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     1681 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     1741 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     1801 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     1861 catggatcca gaactcgtaa acttcgtaac cgccttagtc ccttcagaaa cagcatgttt
     1921 cgccaactct ccaggcaaca caagtctcac cgcagtctga atctccctag aagtaatcgt
     1981 cggcttcttg ttgtacctcg caagcttcga agactcacca gcaagtttct caaagatatc
     2041 attgatgaaa ctgttcatga ttcccatggc tttgctggag attccgatat ctggatgaac
     2101 ttgcttcaac accttgaaga tgtagatctt gtatgtctca acgttcttct ttgatctctt
     2161 cttcttcttg tctccggcgc cggctggttc cttcgggagt ttctttccgg cttttggttt
     2221 cttctctgcc gctgctgctg gttcggctgc cggagttttc tctgccggtt tcttctccgc
     2281 tggtttctta tctgccttcg ccatctgtcc tctccaaatg aaatgaactt ccttatatag
     2341 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     2401 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     2461 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     2521 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     2581 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     2641 gaaaagtctc aattgccctt tggtcttctg agactgtatc gatctggatt ttagtactgg
     2701 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     2761 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     2821 tatctgggaa ctactcacac attattatgg agaaattact tgtacagctc gtccatgccg
     2881 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2941 gggtccatga gaccctgatc cccaacgctt cgaatacgtt tctgcagatg ttccaagctc
     3001 tcaactctac gcatcgaagc tgttctgttc atcttgcacc ctatcaaggc cttgcctttg
     3061 ttccccgagc taatgatctc tggtgtagtc acttggctgc ttgtagtgtc tggagaattt
     3121 gatgtctctg aaggaagaga gacagttgaa acaatctgag gcatattgtg gaacattggg
     3181 ttaaagccag tgagtctctt cactgtctct tcagccattt tcacctttgc tcgtagtgtc
     3241 tcaatattgg cttttaaaac tctgttttct acagatgcat cattgaatgt ttgagttaca
     3301 tcagtgagac ccttcatgag ttttgaattc tctacacgaa gctgtgaaac ttgtgtctct
     3361 agctcactca agtgtgcttg ctttcttctt ctggaccgtc tagctgattc tctattagag
     3421 agcatccttt taacgcgttt aacattagta gggttcatat tagtttcacc atcagcttct
     3481 tcttcatcac cagagagctc agatccactt gttatagcac tgctcatcat tggtgtagct
     3541 ttggaagaag caagagaggc ttgttctgat tcattggctc caccattgtc agatctacca
     3601 gaagtatcct gaggtttgat gaaagttccc cttttcatgg cgacagcagc acaagcaaga
     3661 ttaagtttac tcttgagaaa tgctctgtat tcctctgaat ctacaggaac attaggagga
     3721 gaggagacgg aaacaccaca cgccgtcgta gattctccgt cgtctgcagc ggcggaggat
     3781 tcttgtatga aacgattgaa tgcccattcg gaagcactgc gattcaacga cgtcattcca
     3841 ttaaccgacc agtgatggtt accggagatt tcttcgtcgg agaaaacttt ttccatggta
     3901 ccctgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     3961 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     4021 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     4081 gggaccactg tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca
     4141 tttgtaggag ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca
     4201 atggaatccg aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg
     4261 gtcttctgag actgtatcga tctggatttt agtactggat tttggtttta ggaattagaa
     4321 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
     4381 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
     4441 tattatggag aaattagtct ttgctcaggg cggactggta gctcaggtag tggttgtcgg
     4501 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4561 cgccgccgtc ctcgatgttg tggcggatct tgaagttggc cttgatgccg ttcttctgct
     4621 tgtcggcggt gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4681 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4741 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4801 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4861 cggggtagcg ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg
     4921 gcacgggcag cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat
     4981 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     5041 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtctcggatc
     5101 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
     5161 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     5221 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     5281 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     5341 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     5401 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     5461 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     5521 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     5581 caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     5641 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     5701 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     5761 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     5821 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     5881 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc tcacgttgac
     5941 ggaaactgca gaacgatctc agttaggtca tgggaaagct gatattcatc cctctgagac
     6001 tctatataat taaatgcgtt tgggggatta gctaattgag aatccatata gagaaatatg
     6061 gccgcgcagc gacgccggtt gaagaatatg aacaaggaat aagatgaaca aggaagaaga
     6121 tgaacaagca ataatttaat taattattgt taataagcat aaattatgaa aataattttg
     6181 atggtaatga aagtattata aataatattt aggagaaaat attgttaagg aaatatctgg
     6241 ttatgtttat ttgttaaggt atatgggttt ttatagaaac ccaggagcag ctatggtaag
     6301 aaacccagga ctcaaaaaat gcctctcaac ttctctcata tgttgcggag tctggagtcc
     6361 catttatacc atagaaagca ttgggggtat agcatggtaa tttcacatcg gactccatac
     6421 cgcggccatc tatataatat tactgatggc cgcgcgaagt cccggtatgc cttttcagcc
     6481 gttggatctg gagccgtacc tttttcgcgt tcccattttg cccctggagt cccgagctat
     6541 ttacgacccc ctgccaccga gtccccttcg tggagcgctc ttcgtccaac acgtgtccca
     6601 acttttgcgc atttttgact acctgtcgcg cacttgaatt aaagttgccc ttttattttc
     6661 ttccaattat tttgcggctg agtagtctag tggatccccc ggagctccac ttgggatagg
     6721 ttaagaaaat atgtctggct tgtaaacgaa aagaatcggg gtttctaggc attttttaaa
     6781 tagtgaccca ggactcaaaa actagctctc aacttctctc atatgttgtg gagtctggag
     6841 tcctttatat atgtttacgc tatagaaggc atttggggac tctcccgggc ccaccatagg
     6901 actccatacc gcggccatct atataatatt actgatggcc gcgcggattt tggagccccc
     6961 ttttgtcgtt agcgcgacct ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc
     7021 attttgcgcc tgactagcct agatattagc aacaacttcg cggctaagtt gttgaccgtc
     7081 aagtataaat taatgggctt atggcctaat gggtatagag caaaatggca tgcctgcaga
     7141 ctagtgcttt actctgatcc gcagatattt catcaggaaa gagctttgca agttccttca
     7201 ttgtagcatc aataatttct gagtcactac atgcaatcca ttcttctgct ggggcaaaaa
     7261 ctaactccaa catggattgg tttggattgt aatattcctt acatgttaca gacatgtcag
     7321 cataaacact taaaagcggg cttctgctaa agagtagatg atcataggtg ttcttcaatt
     7381 tcctatcaaa ccagatgtga acgttgataa ctggaactcc aactaatttc tctaatttct
     7441 tgaagtatga aatctctctc cagtcttcag gcactagtga attcgctagc gttaactggc
     7501 cataatcctg tgtatgcaac gttgaaaatt cggatctatt tttacgattc gatcatgaat
     7561 taataaaatt taaattttat tgaatgattt tctagtacat aactgacata cgacttatca
     7621 gttgcgaaac gaacagctct aattacattg ttaagtgaaa ttacgcctaa ttcgtctaag
     7681 tacaacataa ccaactgttt aaacctaatt aaataagtcg acccagaagc tgtcagagat
     7741 gtcgtccaga cttggaagtt caggtaagct ttgtggagat gcaacgctct ccgtaagttg
     7801 tggttgaatc ggatctggac gtggtatata cgcgtcctgg tgtatagcag atcctctact
     7861 ccgtatatct tgaaatatag gggatttatt atctcccaga tatacacgcc attctctgcc
     7921 tgacgtgcag tgatgatttc ccctgtgcgt gaatccatga ccggtgcagc ctatgtggaa
     7981 gtatatggtg cacccacagt ttagatcaat gcgtcgtcgt ctaatcgccc tcctcttggc
     8041 ttgcctgtgt gccttcttga tagagggggg cgtcgagagt gatgaagata acattcttga
     8101 gagtccaatg tttgagcgac gcattttccg ctttatccag gaaatcttta tagctggatc
     8161 cctcgcctgg attgcaaagc acgattgaag ggatccctcc tttaatttga actggtttcc
     8221 cgtacttgca atttgactgc cagtcttttt gggccccaag aagctctttc caatgcttta
     8281 gctttaggta ttgcggtgct acgtcatcaa tgacgttata ggcaacatta ttcgagtaca
     8341 ctcgattatt gaagtccagg tgtccactga ggtaattatg tgggcctaaa gcacgagccc
     8401 acatcgtctt gcccgttcta gaatcacctt ccactatgag actaactggt cttacaggcc
     8461 gcgcagcggg acccattcca aaataatcat ccgcccattc ttgcatctgt tcaggaacgt
     8521 tagtgaaaga ggaaagtgga aatggaggga cccattgttc aggcgccttt ttgaaaaggc
     8581 gttccaagtt cgctgtaatg ttatggtaac tcacgatgaa cgcccttgga tctccggccc
     8641 ggataattgc aagagcttcc gccaaactag gtgcattgat ggcgttataa tagacgtcgt
     8701 ctttattctt ctttgtaccc ccaggcactt tgaactgtcc ggattcacaa caatcgccgt
     8761 ctttggtgat gtaattcttg acggcgttgg cgtctttggc tgcttgtatg tttgggtgaa
     8821 aattggtcga ccttctgggg tgagtaaggt cgaaaaatct cttattcttg atgttggact
     8881 ttccagagag ttggatgaga cagtgaaggt gtgggaatcc gtccgagtgc tcctctctgg
     8941 caactcgaat atatgttggt ttgacgactg accatgataa ggtttgaagc atttgaagag
     9001 cttcattttt tgggatatcg cacttgggat aggttaagaa aatatgtctg gcttgtaaac
     9061 gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca aaaactagct
     9121 ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta cgctatagaa
     9181 ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca tctatataat
     9241 attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga cctttaattc
     9301 aaattaaagg ttgctttttt ctatcgtcca atcattttgc gcctgactag cctagatatt
     9361 agcaacaact tcgcgactaa gttgttgacc gtcaagtata aattaatggg cttatggccc
     9421 aatgggtata gagcaaaatg cccaagcgtg atgccccgtg gcgcttatta gcgggtacgt
     9481 ctaaggtaag tcgaaatgcc aactactctc ccttgagagt attgggccct aaagttaata
     9541 agggacctcg agggggggcc cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     9601 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9661 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9721 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     9781 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     9841 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     9901 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     9961 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
    10021 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
    10081 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
    10141 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
    10201 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
    10261 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
    10321 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
    10381 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
    10441 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
    10501 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
    10561 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
    10621 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
    10681 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
    10741 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
    10801 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
    10861 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
    10921 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
    10981 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
    11041 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
    11101 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
    11161 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
    11221 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
    11281 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
    11341 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
    11401 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
    11461 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
    11521 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
    11581 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
    11641 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
    11701 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
    11761 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
    11821 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
    11881 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    11941 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    12001 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    12061 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    12121 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
    12181 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
    12241 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
    12301 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
    12361 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
    12421 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
    12481 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
    12541 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
    12601 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
    12661 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
    12721 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
    12781 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
    12841 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
    12901 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
    12961 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
    13021 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
    13081 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
    13141 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
    13201 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    13261 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    13321 aggggaaaaa ggtcgaaaag gtgtctttcc tgtggatagc acgtacattg ggaacccaaa
    13381 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    13441 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    13501 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    13561 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    13621 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    13681 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    13741 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    13801 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    13861 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    13921 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    13981 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    14041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    14101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    14161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    14221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    14281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    14341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    14401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    14461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    14521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    14581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    14641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    14701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    14761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    14821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
    14881 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    14941 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    15001 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    15061 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    15121 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    15181 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    15241 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    15301 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    15361 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    15421 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    15481 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    15541 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    15601 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    15661 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    15721 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    15781 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    15841 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    15901 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    15961 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    16021 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    16081 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    16141 cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat
    16201 taattcgggg atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    16261 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc
    16321 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    16381 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    16441 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    16501 ggaaacagct atgacatgat tacg
//
