LOCUS       sequence-475763-       12009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for doxycycline inducible expression of nontargeting 
            shRNA, GFP marker, based on Addgene 11652.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12009)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Dox nontargeting shRNA GFP
FEATURES             Location/Qualifiers
     source          1..12009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             75..708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    755..880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1373..1606
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1791..1835
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2102..2219
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2258..2757
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2293..2316)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2690..2709
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2808..2817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2814..2816,2817..2819,2820..3530)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2814..2816,2817..2819,2820..3530)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2859..2880)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3120..3139)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3467..3488
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3608..4175
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3626..4177
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3770..3787)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3997..4016
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4801..4821
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4924..5118
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     primer_bind     complement(5257..5273)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5258..5274)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    5294..5882
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5347..5367)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5765..5776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(6050..6068,6069..6091,6092..6110,6111..6133,6134..6152,
                     6153..6175,6176..6194,6195..6217,6218..6236,6237..6259,
                     6260..6278,6279..6301,6302..6320)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        6349..6563
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6484..6503
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(6645..6678)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6698..6878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6903..6921)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6904..6921)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7211..7230)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7444..7463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7563..7585
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7623..7641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7709..7813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7814..7882,7883..8674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8032..8051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8845..9433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9334..9353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9587..9604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9674..9694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9679..10008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9859..9994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11142..11207
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11337..11357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11782..11916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11819..11838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11873..11892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
       61 acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct
      121 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
      181 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
      241 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
      301 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
      361 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
      421 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
      481 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
      541 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      601 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      661 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      721 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      781 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      841 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      901 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      961 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1021 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1081 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1141 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1201 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1261 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1321 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1381 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1441 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1501 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1561 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1621 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1681 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1741 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1801 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1861 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1921 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1981 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2041 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     2101 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2161 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     2221 tcgatcacga gactagcctc gacgatggtc gagtaccggg taggggaggc gcttttccca
     2281 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     2341 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     2401 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     2461 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     2521 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     2581 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     2641 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     2701 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     2761 tctagcggga tccaggccta agcttacgcg tcctagcgct accggtcgcc accatggtga
     2821 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2881 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2941 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3001 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3061 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3121 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3181 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3241 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3301 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3361 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3421 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3481 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     3541 gatctcgact agctagtagc tagctagcta gtcgagctca agcttcgaat tcccgggctg
     3601 caggaattcc gccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     3661 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     3721 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     3781 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     3841 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     3901 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     3961 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     4021 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4081 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg
     4141 gggacgtggt tttcctttga aaaacacgat gataatacca tggctagact ggacaagagc
     4201 aaagtcataa acggcgctct ggaattactc aatggagtcg gtatcgaagg cctgacgaca
     4261 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     4321 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     4381 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcattccgc
     4441 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     4501 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     4561 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     4621 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     4681 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     4741 ttcggcctgg aactaatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcg
     4801 ccaaaaaaga agagaaaggt cgacggcggt ggtgctttgt ctcctcagca ctctgctgtc
     4861 actcaaggaa gtatcatcaa gaacaaggag ggcatggatg ctaagtcact aactgcctgg
     4921 tcccggacac tggtgacctt caaggatgta tttgtggact tcaccaggga ggagtggaag
     4981 ctgctggaca ctgctcagca gatcgtgtac agaaatgtga tgctggagaa ctataagaac
     5041 ctggtttcct tgggttatca gcttactaag ccagatgtga tcctccggtt ggagaaggga
     5101 gaagagccct ggctggtgga gagagaaatt caccaagaga cccatcctga ttcagagact
     5161 gcatttgaaa tcaaatcatc agtttaagga tctcgactag ctagtagcta gctagctagt
     5221 cgagctcaag cttcgaattc gatatcaagc ttatcgcgat accgtcgacc tcgacctcga
     5281 ctagtcatat gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5341 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5401 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5461 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5521 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5581 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5641 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     5701 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5761 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5821 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5881 gcatcggtac gtatggccag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5941 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6001 aagatgggat caattcacca tgctagtgga tccccctcga gtttaccact ccctatcagt
     6061 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     6121 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     6181 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     6241 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     6301 ctccctatca gtgatagaga aaagtgaaag tcggggctgc aggaattcga acgctgacgt
     6361 catcaacccg ctccaaggaa tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc
     6421 gtgcgccctg gcaggaagat ggctgtgagg gacaggggag tggcgccctg caatatttgc
     6481 atgtcgctat gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc
     6541 ttataagttc tgtatgagac cacgcgccct ctcgcttggg cgagagtaag ttcaagagac
     6601 ttactctcgc ccaagcgaga tttttacgat accgtcgcat gggaataact tcgtatagca
     6661 tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6721 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6781 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6841 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     6901 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     6961 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     7021 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     7081 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     7141 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     7201 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     7261 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     7321 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     7381 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     7441 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     7501 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     7561 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     7621 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7681 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7801 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7861 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7921 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7981 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8041 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8101 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8161 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8221 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8281 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8341 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8401 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8461 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8521 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9541 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9601 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9661 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9781 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9841 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9901 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9961 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
    10021 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
    10081 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
    10141 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
    10201 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
    10261 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
    10321 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
    10381 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
    10441 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
    10501 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
    10561 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
    10621 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    10681 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    10741 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    10801 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    10861 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    10921 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    10981 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    11041 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    11101 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    11161 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    11221 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    11281 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    11341 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    11401 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    11461 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    11521 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    11581 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    11641 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    11701 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    11761 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    11821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11881 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11941 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    12001 ttactctaa
//
