LOCUS       sequence-475766-       16503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one bimolecular fluorescence complementation (BiFC) 
            vector is used to study the self-interation of AtbZIP63 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16503)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 16503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTHV3-(AtbZIP63-nV+cV)
FEATURES             Location/Qualifiers
     source          1..16503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1153..1863)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1431..1450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2306..2651)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2681..2855
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2901..2922)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(2962..3307)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3337..3511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(3629..4141,4142..4144,4145..4147))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     3822..3841
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4081..4102
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(5143..5819)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9589..9606)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9589..9605)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9598..9620)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9808..9832
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11133..11762
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12191..13264
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13330..13524
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13760..13782)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13868..14008
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13923..13942
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14023..14040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14194..14782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14274..14293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14869..15663)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16088..16112
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    16380..16401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16416..16421,16422..16439,16440..16446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16454..16470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     16459..16481
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     16478..16494
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     16478..16494
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     1201 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     1261 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     1321 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     1381 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     1441 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     1501 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     1561 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     1621 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     1681 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     1741 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     1801 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     1861 catggatcca gaactcgtaa acttcgtaac cgccttagtc ccttcagaaa cagcatgttt
     1921 cgccaactct ccaggcaaca caagtctcac cgcagtctga atctccctag aagtaatcgt
     1981 cggcttcttg ttgtacctcg caagcttcga agactcacca gcaagtttct caaagatatc
     2041 attgatgaaa ctgttcatga ttcccatggc tttgctggag attccgatat ctggatgaac
     2101 ttgcttcaac accttgaaga tgtagatctt gtatgtctca acgttcttct ttgatctctt
     2161 cttcttcttg tctccggcgc cggctggttc cttcgggagt ttctttccgg cttttggttt
     2221 cttctctgcc gctgctgctg gttcggctgc cggagttttc tctgccggtt tcttctccgc
     2281 tggtttctta tctgccttcg ccatctgtcc tctccaaatg aaatgaactt ccttatatag
     2341 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     2401 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     2461 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     2521 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     2581 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     2641 gaaaagtctc aattgccctt tggtcttctg agactgtatc gatctggatt ttagtactgg
     2701 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     2761 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     2821 tatctgggaa ctactcacac attattatgg agaaattact tgtacagctc gtccatgccg
     2881 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2941 gggtccatga gaccggtacc ctgtcctctc caaatgaaat gaacttcctt atatagagga
     3001 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     3061 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     3121 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga tggcctttcc
     3181 tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct tcacaataaa
     3241 gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc tttgttgaaa
     3301 agtctcaatt gccctttggt cttctgagac tgtatcgatc tggattttag tactggattt
     3361 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     3421 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     3481 tgggaactac tcacacatta ttatggagaa attagtcttt gctcagggcg gactggtagc
     3541 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3601 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
     3661 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
     3721 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     3781 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3841 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     3901 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
     3961 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
     4021 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4081 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4141 tcaccatctg atccccaacg cttcgaatac gtttctgcag atgttccaag ctctcaactc
     4201 tacgcatcga agctgttctg ttcatcttgc accctatcaa ggccttgcct ttgttccccg
     4261 agctaatgat ctctggtgta gtcacttggc tgcttgtagt gtctggagaa tttgatgtct
     4321 ctgaaggaag agagacagtt gaaacaatct gaggcatatt gtggaacatt gggttaaagc
     4381 cagtgagtct cttcactgtc tcttcagcca ttttcacctt tgctcgtagt gtctcaatat
     4441 tggcttttaa aactctgttt tctacagatg catcattgaa tgtttgagtt acatcagtga
     4501 gacccttcat gagttttgaa ttctctacac gaagctgtga aacttgtgtc tctagctcac
     4561 tcaagtgtgc ttgctttctt cttctggacc gtctagctga ttctctatta gagagcatcc
     4621 ttttaacgcg tttaacatta gtagggttca tattagtttc accatcagct tcttcttcat
     4681 caccagagag ctcagatcca cttgttatag cactgctcat cattggtgta gctttggaag
     4741 aagcaagaga ggcttgttct gattcattgg ctccaccatt gtcagatcta ccagaagtat
     4801 cccttttcat ggcgacagca gcacaagcaa gattaagttt actcttgaga aatgctctgt
     4861 attcctctga atctacagga acattaggag gagaggagac ggaaacacca cacgccgtcg
     4921 tagattctcc gtcgtctgca gcggcggagg attcttgtat gaaacgattg aatgcccatt
     4981 cggaagcact gcgattcaac gacgtcattc cattaaccga ccagtgatgg ttaccggaga
     5041 tttcttcgtc ggagaaaact ttttccatgg tctcggatct cattgccccc cggatctgcg
     5101 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
     5161 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     5221 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     5281 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     5341 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     5401 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     5461 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     5521 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca
     5581 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     5641 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     5701 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     5761 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
     5821 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
     5881 gctccaccat gttggcaagc tgctctagct cacgttgacg gaaactgcag aacgatctca
     5941 gttaggtcat gggaaagctg atattcatcc ctctgagact ctatataatt aaatgcgttt
     6001 gggggattag ctaattgaga atccatatag agaaatatgg ccgcgcagcg acgccggttg
     6061 aagaatatga acaaggaata agatgaacaa ggaagaagat gaacaagcaa taatttaatt
     6121 aattattgtt aataagcata aattatgaaa ataattttga tggtaatgaa agtattataa
     6181 ataatattta ggagaaaata ttgttaagga aatatctggt tatgtttatt tgttaaggta
     6241 tatgggtttt tatagaaacc caggagcagc tatggtaaga aacccaggac tcaaaaaatg
     6301 cctctcaact tctctcatat gttgcggagt ctggagtccc atttatacca tagaaagcat
     6361 tgggggtata gcatggtaat ttcacatcgg actccatacc gcggccatct atataatatt
     6421 actgatggcc gcgcgaagtc ccggtatgcc ttttcagccg ttggatctgg agccgtacct
     6481 ttttcgcgtt cccattttgc ccctggagtc ccgagctatt tacgaccccc tgccaccgag
     6541 tccccttcgt ggagcgctct tcgtccaaca cgtgtcccaa cttttgcgca tttttgacta
     6601 cctgtcgcgc acttgaatta aagttgccct tttattttct tccaattatt ttgcggctga
     6661 gtagtctagt ggatcccccg gagctccact tgggataggt taagaaaata tgtctggctt
     6721 gtaaacgaaa agaatcgggg tttctaggca ttttttaaat agtgacccag gactcaaaaa
     6781 ctagctctca acttctctca tatgttgtgg agtctggagt cctttatata tgtttacgct
     6841 atagaaggca tttggggact ctcccgggcc caccatagga ctccataccg cggccatcta
     6901 tataatatta ctgatggccg cgcggatttt ggagccccct tttgtcgtta gcgcgacctt
     6961 taattcaaat taaaggttgc ttttttctat cgtccgatca ttttgcgcct gactagccta
     7021 gatattagca acaacttcgc ggctaagttg ttgaccgtca agtataaatt aatgggctta
     7081 tggcctaatg ggtatagagc aaaatggcat gcctgcagac tagtgcttta ctctgatccg
     7141 cagatatttc atcaggaaag agctttgcaa gttccttcat tgtagcatca ataatttctg
     7201 agtcactaca tgcaatccat tcttctgctg gggcaaaaac taactccaac atggattggt
     7261 ttggattgta atattcctta catgttacag acatgtcagc ataaacactt aaaagcgggc
     7321 ttctgctaaa gagtagatga tcataggtgt tcttcaattt cctatcaaac cagatgtgaa
     7381 cgttgataac tggaactcca actaatttct ctaatttctt gaagtatgaa atctctctcc
     7441 agtcttcagg cactagtgaa ttcgctagcg ttaactggcc ataatcctgt gtatgcaacg
     7501 ttgaaaattc ggatctattt ttacgattcg atcatgaatt aataaaattt aaattttatt
     7561 gaatgatttt ctagtacata actgacatac gacttatcag ttgcgaaacg aacagctcta
     7621 attacattgt taagtgaaat tacgcctaat tcgtctaagt acaacataac caactgttta
     7681 aacctaatta aataagtcga cccagaagct gtcagagatg tcgtccagac ttggaagttc
     7741 aggtaagctt tgtggagatg caacgctctc cgtaagttgt ggttgaatcg gatctggacg
     7801 tggtatatac gcgtcctggt gtatagcaga tcctctactc cgtatatctt gaaatatagg
     7861 ggatttatta tctcccagat atacacgcca ttctctgcct gacgtgcagt gatgatttcc
     7921 cctgtgcgtg aatccatgac cggtgcagcc tatgtggaag tatatggtgc acccacagtt
     7981 tagatcaatg cgtcgtcgtc taatcgccct cctcttggct tgcctgtgtg ccttcttgat
     8041 agaggggggc gtcgagagtg atgaagataa cattcttgag agtccaatgt ttgagcgacg
     8101 cattttccgc tttatccagg aaatctttat agctggatcc ctcgcctgga ttgcaaagca
     8161 cgattgaagg gatccctcct ttaatttgaa ctggtttccc gtacttgcaa tttgactgcc
     8221 agtctttttg ggccccaaga agctctttcc aatgctttag ctttaggtat tgcggtgcta
     8281 cgtcatcaat gacgttatag gcaacattat tcgagtacac tcgattattg aagtccaggt
     8341 gtccactgag gtaattatgt gggcctaaag cacgagccca catcgtcttg cccgttctag
     8401 aatcaccttc cactatgaga ctaactggtc ttacaggccg cgcagcggga cccattccaa
     8461 aataatcatc cgcccattct tgcatctgtt caggaacgtt agtgaaagag gaaagtggaa
     8521 atggagggac ccattgttca ggcgcctttt tgaaaaggcg ttccaagttc gctgtaatgt
     8581 tatggtaact cacgatgaac gcccttggat ctccggcccg gataattgca agagcttccg
     8641 ccaaactagg tgcattgatg gcgttataat agacgtcgtc tttattcttc tttgtacccc
     8701 caggcacttt gaactgtccg gattcacaac aatcgccgtc tttggtgatg taattcttga
     8761 cggcgttggc gtctttggct gcttgtatgt ttgggtgaaa attggtcgac cttctggggt
     8821 gagtaaggtc gaaaaatctc ttattcttga tgttggactt tccagagagt tggatgagac
     8881 agtgaaggtg tgggaatccg tccgagtgct cctctctggc aactcgaata tatgttggtt
     8941 tgacgactga ccatgataag gtttgaagca tttgaagagc ttcatttttt gggatatcgc
     9001 acttgggata ggttaagaaa atatgtctgg cttgtaaacg aaaagaatcg gggtttctag
     9061 gcatttttta aatagtgacc caggactcaa aaactagctc tcaacttctc tcatatgttg
     9121 tggagtctgg agtcctttat atatgtttac gctatagaag gcatttgggg actctcccgg
     9181 gcccaccata ggactccata ccgcggccat ctatataata ttactgatgg ccgcgcggat
     9241 tttggagccc ccttttgtcg ttagcgcgac ctttaattca aattaaaggt tgcttttttc
     9301 tatcgtccaa tcattttgcg cctgactagc ctagatatta gcaacaactt cgcgactaag
     9361 ttgttgaccg tcaagtataa attaatgggc ttatggccca atgggtatag agcaaaatgc
     9421 ccaagcgtga tgccccgtgg cgcttattag cgggtacgtc taaggtaagt cgaaatgcca
     9481 actactctcc cttgagagta ttgggcccta aagttaataa gggacctcga gggggggccc
     9541 ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt
     9601 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9661 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9721 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     9781 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     9841 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     9901 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     9961 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
    10021 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
    10081 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
    10141 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
    10201 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
    10261 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
    10321 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
    10381 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
    10441 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
    10501 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
    10561 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
    10621 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
    10681 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
    10741 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
    10801 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
    10861 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
    10921 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
    10981 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
    11041 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
    11101 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
    11161 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
    11221 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
    11281 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
    11341 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
    11401 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
    11461 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
    11521 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
    11581 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
    11641 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
    11701 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
    11761 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
    11821 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
    11881 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
    11941 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
    12001 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
    12061 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
    12121 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
    12181 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    12241 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    12301 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    12361 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    12421 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    12481 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    12541 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    12601 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    12661 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    12721 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    12781 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    12841 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    12901 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    12961 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    13021 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    13081 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    13141 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    13201 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    13261 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    13321 tgtctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    13381 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    13441 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    13501 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    13561 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    13621 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    13681 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    13741 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    13801 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    13861 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    13921 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    13981 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    14041 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    14101 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    14161 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    14221 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    14281 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    14341 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    14401 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    14461 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    14521 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    14581 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    14641 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    14701 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    14761 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    14821 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
    14881 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    14941 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    15001 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    15061 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    15121 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    15181 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    15241 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    15301 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    15361 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    15421 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    15481 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    15541 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    15601 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    15661 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    15721 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    15781 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    15841 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    15901 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
    15961 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
    16021 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    16081 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
    16141 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcgggga tagccctttg
    16201 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
    16261 gttggcaagc tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    16321 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    16381 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    16441 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    16501 acg
//
