LOCUS       sequence-475767-       14708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one subcellar localization vector. Construct containing EGFP 
            tag and cell endoplasmic reticulum-targeted mCherry for 
            co-localization study in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTCL3
FEATURES             Location/Qualifiers
     source          1..14708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1180..1887)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1455..1474)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(1988..2333)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2363..2537
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(2545..3258,3259..3261,3262..3264))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2548..3258,3259..3261,3262..3264))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2590..2611)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2939..2958
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3198..3219
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3348..4024)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7794..7811)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7794..7810)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7803..7825)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8013..8037
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9338..9967
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10396..11469
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11535..11729
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11965..11987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12073..12213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12128..12147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12228..12245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12399..12987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12479..12498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13074..13868)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14293..14317
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    14585..14606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14621..14626,14627..14644,14645..14651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14659..14675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14664..14686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14683..14699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14683..14699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcggtaccct aaagctcatc atgacctgac ttgtacagct cgtccatgcc
     1201 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
     1261 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
     1321 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
     1381 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
     1441 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
     1501 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
     1561 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     1621 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
     1681 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
     1741 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     1801 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
     1861 gttatcctcc tcgcccttgc tcaccatgga tccgcactcc ttgcgaggtt gccccttgac
     1921 tagctgcgta taagcaaggt agaaaacagc caccacgaac aaaccctcct gtaccttcat
     1981 ggtaccctgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     2041 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     2101 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     2161 ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta tcgcaatgat
     2221 ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga cagatagctg
     2281 ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc tcaattgccc
     2341 tttggtcttc tgagactgta tcgatctgga ttttagtact ggattttggt tttaggaatt
     2401 agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg tttcttatat
     2461 gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg aactactcac
     2521 acattattat ggagaaactc gagcttactt gtacagctcg tccatgccga gagtgatccc
     2581 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     2641 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     2701 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     2761 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
     2821 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     2881 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     2941 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
     3001 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     3061 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     3121 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     3181 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     3241 cagctcctcg cccttgctca ccatgagacc tacggtctcg gatctcattg ccccccggat
     3301 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     3361 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     3421 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     3481 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     3541 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     3601 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     3661 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     3721 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     3781 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     3841 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     3901 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     3961 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     4021 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     4081 gtcgtgctcc accatgttgg caagctgctc tagctcacgt tgacggaaac tgcagaacga
     4141 tctcagttag gtcatgggaa agctgatatt catccctctg agactctata taattaaatg
     4201 cgtttggggg attagctaat tgagaatcca tatagagaaa tatggccgcg cagcgacgcc
     4261 ggttgaagaa tatgaacaag gaataagatg aacaaggaag aagatgaaca agcaataatt
     4321 taattaatta ttgttaataa gcataaatta tgaaaataat tttgatggta atgaaagtat
     4381 tataaataat atttaggaga aaatattgtt aaggaaatat ctggttatgt ttatttgtta
     4441 aggtatatgg gtttttatag aaacccagga gcagctatgg taagaaaccc aggactcaaa
     4501 aaatgcctct caacttctct catatgttgc ggagtctgga gtcccattta taccatagaa
     4561 agcattgggg gtatagcatg gtaatttcac atcggactcc ataccgcggc catctatata
     4621 atattactga tggccgcgcg aagtcccggt atgccttttc agccgttgga tctggagccg
     4681 tacctttttc gcgttcccat tttgcccctg gagtcccgag ctatttacga ccccctgcca
     4741 ccgagtcccc ttcgtggagc gctcttcgtc caacacgtgt cccaactttt gcgcattttt
     4801 gactacctgt cgcgcacttg aattaaagtt gcccttttat tttcttccaa ttattttgcg
     4861 gctgagtagt ctagtggatc ccccggagct ccacttggga taggttaaga aaatatgtct
     4921 ggcttgtaaa cgaaaagaat cggggtttct aggcattttt taaatagtga cccaggactc
     4981 aaaaactagc tctcaacttc tctcatatgt tgtggagtct ggagtccttt atatatgttt
     5041 acgctataga aggcatttgg ggactctccc gggcccacca taggactcca taccgcggcc
     5101 atctatataa tattactgat ggccgcgcgg attttggagc ccccttttgt cgttagcgcg
     5161 acctttaatt caaattaaag gttgcttttt tctatcgtcc gatcattttg cgcctgacta
     5221 gcctagatat tagcaacaac ttcgcggcta agttgttgac cgtcaagtat aaattaatgg
     5281 gcttatggcc taatgggtat agagcaaaat ggcatgcctg cagactagtg ctttactctg
     5341 atccgcagat atttcatcag gaaagagctt tgcaagttcc ttcattgtag catcaataat
     5401 ttctgagtca ctacatgcaa tccattcttc tgctggggca aaaactaact ccaacatgga
     5461 ttggtttgga ttgtaatatt ccttacatgt tacagacatg tcagcataaa cacttaaaag
     5521 cgggcttctg ctaaagagta gatgatcata ggtgttcttc aatttcctat caaaccagat
     5581 gtgaacgttg ataactggaa ctccaactaa tttctctaat ttcttgaagt atgaaatctc
     5641 tctccagtct tcaggcacta gtgaattcgc tagcgttaac tggccataat cctgtgtatg
     5701 caacgttgaa aattcggatc tatttttacg attcgatcat gaattaataa aatttaaatt
     5761 ttattgaatg attttctagt acataactga catacgactt atcagttgcg aaacgaacag
     5821 ctctaattac attgttaagt gaaattacgc ctaattcgtc taagtacaac ataaccaact
     5881 gtttaaacct aattaaataa gtcgacccag aagctgtcag agatgtcgtc cagacttgga
     5941 agttcaggta agctttgtgg agatgcaacg ctctccgtaa gttgtggttg aatcggatct
     6001 ggacgtggta tatacgcgtc ctggtgtata gcagatcctc tactccgtat atcttgaaat
     6061 ataggggatt tattatctcc cagatataca cgccattctc tgcctgacgt gcagtgatga
     6121 tttcccctgt gcgtgaatcc atgaccggtg cagcctatgt ggaagtatat ggtgcaccca
     6181 cagtttagat caatgcgtcg tcgtctaatc gccctcctct tggcttgcct gtgtgccttc
     6241 ttgatagagg ggggcgtcga gagtgatgaa gataacattc ttgagagtcc aatgtttgag
     6301 cgacgcattt tccgctttat ccaggaaatc tttatagctg gatccctcgc ctggattgca
     6361 aagcacgatt gaagggatcc ctcctttaat ttgaactggt ttcccgtact tgcaatttga
     6421 ctgccagtct ttttgggccc caagaagctc tttccaatgc tttagcttta ggtattgcgg
     6481 tgctacgtca tcaatgacgt tataggcaac attattcgag tacactcgat tattgaagtc
     6541 caggtgtcca ctgaggtaat tatgtgggcc taaagcacga gcccacatcg tcttgcccgt
     6601 tctagaatca ccttccacta tgagactaac tggtcttaca ggccgcgcag cgggacccat
     6661 tccaaaataa tcatccgccc attcttgcat ctgttcagga acgttagtga aagaggaaag
     6721 tggaaatgga gggacccatt gttcaggcgc ctttttgaaa aggcgttcca agttcgctgt
     6781 aatgttatgg taactcacga tgaacgccct tggatctccg gcccggataa ttgcaagagc
     6841 ttccgccaaa ctaggtgcat tgatggcgtt ataatagacg tcgtctttat tcttctttgt
     6901 acccccaggc actttgaact gtccggattc acaacaatcg ccgtctttgg tgatgtaatt
     6961 cttgacggcg ttggcgtctt tggctgcttg tatgtttggg tgaaaattgg tcgaccttct
     7021 ggggtgagta aggtcgaaaa atctcttatt cttgatgttg gactttccag agagttggat
     7081 gagacagtga aggtgtggga atccgtccga gtgctcctct ctggcaactc gaatatatgt
     7141 tggtttgacg actgaccatg ataaggtttg aagcatttga agagcttcat tttttgggat
     7201 atcgcacttg ggataggtta agaaaatatg tctggcttgt aaacgaaaag aatcggggtt
     7261 tctaggcatt ttttaaatag tgacccagga ctcaaaaact agctctcaac ttctctcata
     7321 tgttgtggag tctggagtcc tttatatatg tttacgctat agaaggcatt tggggactct
     7381 cccgggccca ccataggact ccataccgcg gccatctata taatattact gatggccgcg
     7441 cggattttgg agcccccttt tgtcgttagc gcgaccttta attcaaatta aaggttgctt
     7501 ttttctatcg tccaatcatt ttgcgcctga ctagcctaga tattagcaac aacttcgcga
     7561 ctaagttgtt gaccgtcaag tataaattaa tgggcttatg gcccaatggg tatagagcaa
     7621 aatgcccaag cgtgatgccc cgtggcgctt attagcgggt acgtctaagg taagtcgaaa
     7681 tgccaactac tctcccttga gagtattggg ccctaaagtt aataagggac ctcgaggggg
     7741 ggcccggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
     7801 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     7861 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     7921 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
     7981 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     8041 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     8101 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     8161 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     8221 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     8281 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     8341 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     8401 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     8461 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     8521 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     8581 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     8641 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     8701 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     8761 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     8821 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     8881 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     8941 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     9001 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     9061 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     9121 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     9181 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     9241 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     9301 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     9361 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     9421 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     9481 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     9541 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     9601 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     9661 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     9721 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     9781 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     9841 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     9901 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     9961 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    10021 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    10081 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    10141 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    10201 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    10261 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    10321 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
    10381 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    10441 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    10501 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    10561 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    10621 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    10681 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    10741 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    10801 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    10861 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    10921 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    10981 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    11041 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    11101 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    11161 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    11221 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    11281 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    11341 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    11401 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    11461 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    11521 aaaggtgtct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    11581 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    11641 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    11701 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
    11761 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    11821 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    11881 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    11941 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    12001 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    12061 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    12121 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    12181 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
    12241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    12301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    12361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    12421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    12481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    12541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    12601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    12661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    12721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    12781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    12841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    12901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    12961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    13021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gkactaaaac
    13081 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    13141 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    13201 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    13261 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    13321 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    13381 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    13441 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    13501 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    13561 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    13621 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    13681 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    13741 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    13801 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    13861 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    13921 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    13981 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    14041 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    14101 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    14161 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    14221 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    14281 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    14341 taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc ggggatagcc
    14401 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    14461 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga
    14521 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    14581 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    14641 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    14701 tgattacg
//
