LOCUS       sequence-475768-       14749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one subcellar localization vector. Construct containing EGFP 
            tag and Golgi-targeted mCherry for co-localization study in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14749)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 14749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTCL4
FEATURES             Location/Qualifiers
     source          1..14749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1164..1874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1442..1461)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2029..2374)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2404..2578
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(2586..3299,3300..3302,3303..3305))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2589..3299,3300..3302,3303..3305))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2631..2652)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2980..2999
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3239..3260
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3389..4065)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7835..7852)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7835..7851)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7844..7866)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8054..8078
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9379..10008
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10437..11510
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11576..11770
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12006..12028)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12114..12254
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12169..12188
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12269..12286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12440..13028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12520..12539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13115..13909)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14334..14358
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    14626..14647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14662..14667,14668..14685,14686..14692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14700..14716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14705..14727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     14724..14740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14724..14740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcggtaccct agattacttg tacagctcgt ccatgccgcc ggtggagtgg
     1201 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
     1261 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
     1321 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
     1381 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
     1441 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
     1501 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
     1561 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
     1621 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
     1681 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     1741 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     1801 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     1861 cccttgctca ccatagtttg acggtcccag aaaacgaaag agacacaaac gaaaacgatg
     1921 aagagcagag caagacgctt tgggcgcttc aagtaatagg aagggttgca gtacctccat
     1981 ttgctgctgc tgctacccac tgatctgctc cctctcgcca tggtaccctg tcctctccaa
     2041 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
     2101 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
     2161 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     2221 agaggcatct tcaacgatgg cctttccttt atcgcaatga tggcatttgt aggagccacc
     2281 ttccttttcc actatcttca caataaagtg acagatagct gggcaatgga atccgaggag
     2341 gtttccggat attacccttt gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt
     2401 atcgatctgg attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa
     2461 gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa
     2521 ccctatagga accctaattc ccttatctgg gaactactca cacattatta tggagaaact
     2581 cgagcttact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     2641 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
     2701 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     2761 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     2821 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     2881 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     2941 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     3001 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     3061 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     3121 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     3181 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     3241 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     3301 accatgagac ctacggtctc ggatctcatt gccccccgga tctgcgaaag ctcgagagag
     3361 atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc
     3421 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     3481 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     3541 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     3601 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     3661 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     3721 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     3781 tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac
     3841 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     3901 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     3961 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     4021 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     4081 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
     4141 gcaagctgct ctagctcacg ttgacggaaa ctgcagaacg atctcagtta ggtcatggga
     4201 aagctgatat tcatccctct gagactctat ataattaaat gcgtttgggg gattagctaa
     4261 ttgagaatcc atatagagaa atatggccgc gcagcgacgc cggttgaaga atatgaacaa
     4321 ggaataagat gaacaaggaa gaagatgaac aagcaataat ttaattaatt attgttaata
     4381 agcataaatt atgaaaataa ttttgatggt aatgaaagta ttataaataa tatttaggag
     4441 aaaatattgt taaggaaata tctggttatg tttatttgtt aaggtatatg ggtttttata
     4501 gaaacccagg agcagctatg gtaagaaacc caggactcaa aaaatgcctc tcaacttctc
     4561 tcatatgttg cggagtctgg agtcccattt ataccataga aagcattggg ggtatagcat
     4621 ggtaatttca catcggactc cataccgcgg ccatctatat aatattactg atggccgcgc
     4681 gaagtcccgg tatgcctttt cagccgttgg atctggagcc gtaccttttt cgcgttccca
     4741 ttttgcccct ggagtcccga gctatttacg accccctgcc accgagtccc cttcgtggag
     4801 cgctcttcgt ccaacacgtg tcccaacttt tgcgcatttt tgactacctg tcgcgcactt
     4861 gaattaaagt tgccctttta ttttcttcca attattttgc ggctgagtag tctagtggat
     4921 cccccggagc tccacttggg ataggttaag aaaatatgtc tggcttgtaa acgaaaagaa
     4981 tcggggtttc taggcatttt ttaaatagtg acccaggact caaaaactag ctctcaactt
     5041 ctctcatatg ttgtggagtc tggagtcctt tatatatgtt tacgctatag aaggcatttg
     5101 gggactctcc cgggcccacc ataggactcc ataccgcggc catctatata atattactga
     5161 tggccgcgcg gattttggag cccccttttg tcgttagcgc gacctttaat tcaaattaaa
     5221 ggttgctttt ttctatcgtc cgatcatttt gcgcctgact agcctagata ttagcaacaa
     5281 cttcgcggct aagttgttga ccgtcaagta taaattaatg ggcttatggc ctaatgggta
     5341 tagagcaaaa tggcatgcct gcagactagt gctttactct gatccgcaga tatttcatca
     5401 ggaaagagct ttgcaagttc cttcattgta gcatcaataa tttctgagtc actacatgca
     5461 atccattctt ctgctggggc aaaaactaac tccaacatgg attggtttgg attgtaatat
     5521 tccttacatg ttacagacat gtcagcataa acacttaaaa gcgggcttct gctaaagagt
     5581 agatgatcat aggtgttctt caatttccta tcaaaccaga tgtgaacgtt gataactgga
     5641 actccaacta atttctctaa tttcttgaag tatgaaatct ctctccagtc ttcaggcact
     5701 agtgaattcg ctagcgttaa ctggccataa tcctgtgtat gcaacgttga aaattcggat
     5761 ctatttttac gattcgatca tgaattaata aaatttaaat tttattgaat gattttctag
     5821 tacataactg acatacgact tatcagttgc gaaacgaaca gctctaatta cattgttaag
     5881 tgaaattacg cctaattcgt ctaagtacaa cataaccaac tgtttaaacc taattaaata
     5941 agtcgaccca gaagctgtca gagatgtcgt ccagacttgg aagttcaggt aagctttgtg
     6001 gagatgcaac gctctccgta agttgtggtt gaatcggatc tggacgtggt atatacgcgt
     6061 cctggtgtat agcagatcct ctactccgta tatcttgaaa tataggggat ttattatctc
     6121 ccagatatac acgccattct ctgcctgacg tgcagtgatg atttcccctg tgcgtgaatc
     6181 catgaccggt gcagcctatg tggaagtata tggtgcaccc acagtttaga tcaatgcgtc
     6241 gtcgtctaat cgccctcctc ttggcttgcc tgtgtgcctt cttgatagag gggggcgtcg
     6301 agagtgatga agataacatt cttgagagtc caatgtttga gcgacgcatt ttccgcttta
     6361 tccaggaaat ctttatagct ggatccctcg cctggattgc aaagcacgat tgaagggatc
     6421 cctcctttaa tttgaactgg tttcccgtac ttgcaatttg actgccagtc tttttgggcc
     6481 ccaagaagct ctttccaatg ctttagcttt aggtattgcg gtgctacgtc atcaatgacg
     6541 ttataggcaa cattattcga gtacactcga ttattgaagt ccaggtgtcc actgaggtaa
     6601 ttatgtgggc ctaaagcacg agcccacatc gtcttgcccg ttctagaatc accttccact
     6661 atgagactaa ctggtcttac aggccgcgca gcgggaccca ttccaaaata atcatccgcc
     6721 cattcttgca tctgttcagg aacgttagtg aaagaggaaa gtggaaatgg agggacccat
     6781 tgttcaggcg cctttttgaa aaggcgttcc aagttcgctg taatgttatg gtaactcacg
     6841 atgaacgccc ttggatctcc ggcccggata attgcaagag cttccgccaa actaggtgca
     6901 ttgatggcgt tataatagac gtcgtcttta ttcttctttg tacccccagg cactttgaac
     6961 tgtccggatt cacaacaatc gccgtctttg gtgatgtaat tcttgacggc gttggcgtct
     7021 ttggctgctt gtatgtttgg gtgaaaattg gtcgaccttc tggggtgagt aaggtcgaaa
     7081 aatctcttat tcttgatgtt ggactttcca gagagttgga tgagacagtg aaggtgtggg
     7141 aatccgtccg agtgctcctc tctggcaact cgaatatatg ttggtttgac gactgaccat
     7201 gataaggttt gaagcatttg aagagcttca ttttttggga tatcgcactt gggataggtt
     7261 aagaaaatat gtctggcttg taaacgaaaa gaatcggggt ttctaggcat tttttaaata
     7321 gtgacccagg actcaaaaac tagctctcaa cttctctcat atgttgtgga gtctggagtc
     7381 ctttatatat gtttacgcta tagaaggcat ttggggactc tcccgggccc accataggac
     7441 tccataccgc ggccatctat ataatattac tgatggccgc gcggattttg gagccccctt
     7501 ttgtcgttag cgcgaccttt aattcaaatt aaaggttgct tttttctatc gtccaatcat
     7561 tttgcgcctg actagcctag atattagcaa caacttcgcg actaagttgt tgaccgtcaa
     7621 gtataaatta atgggcttat ggcccaatgg gtatagagca aaatgcccaa gcgtgatgcc
     7681 ccgtggcgct tattagcggg tacgtctaag gtaagtcgaa atgccaacta ctctcccttg
     7741 agagtattgg gccctaaagt taataaggga cctcgagggg gggcccggta cccggggatc
     7801 ctctagagtc gacctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg
     7861 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7921 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7981 atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     8041 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     8101 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     8161 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     8221 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     8281 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     8341 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     8401 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     8461 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     8521 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     8581 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     8641 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     8701 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     8761 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     8821 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     8881 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     8941 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     9001 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     9061 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     9121 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     9181 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     9241 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     9301 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     9361 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     9421 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     9481 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     9541 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     9601 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     9661 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     9721 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     9781 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     9841 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     9901 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     9961 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
    10021 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
    10081 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
    10141 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
    10201 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
    10261 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
    10321 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
    10381 tgggttgcct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
    10441 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
    10501 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
    10561 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
    10621 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
    10681 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
    10741 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
    10801 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
    10861 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
    10921 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
    10981 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
    11041 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
    11101 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
    11161 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
    11221 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
    11281 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
    11341 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    11401 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    11461 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    11521 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtgtc tttcctgtgg
    11581 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    11641 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    11701 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    11761 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    11821 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
    11881 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
    11941 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
    12001 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
    12061 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
    12121 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
    12181 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    12241 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    12301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    12361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    12421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    12481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    12541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    12601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    12661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    12721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    12781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    12901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    13021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    13081 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
    13141 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    13201 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    13261 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    13321 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    13381 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    13441 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    13501 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    13561 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    13621 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    13681 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    13741 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    13801 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    13861 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    13921 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    13981 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    14041 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
    14101 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
    14161 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
    14221 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
    14281 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    14341 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    14401 ttaatgtact gaattaacgc cgaattaatt cggggatagc cctttggtct tctgagactg
    14461 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct
    14521 ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    14581 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    14641 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    14701 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacg
//
