LOCUS       sequence-475771-        7294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding long isoform of human DIXDC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ
  TITLE     An AMPK-independent signaling pathway downstream of the LKB1 tumor 
            suppressor controls Snail1 and metastatic potential.
  JOURNAL   Mol Cell. 2014 Aug 7;55(3):436-50. doi: 
            10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17.
  PUBMED    25042806
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.06.021"; journalName: "Mol Cell"; date: 
            "2014-08-7- 7"; volume: "55"; issue: "3"; pages: "436-50"
COMMENT     Sequence Label: pcDNA3-FLAG-hDIXDC1 WT
FEATURES             Location/Qualifiers
     source          1..7294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    915..939
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             948..971
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(3077..3101)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3115..3156
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3175..3192
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     primer_bind     complement(3232..3251)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3276..3324
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3526..3954
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3613..3632)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3823..3844
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3963..3983)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3968..4297
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4148..4283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4210..4229
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4345..4392
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4411..4809
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4967..5088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5004..5023)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5058..5077
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5137..5153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5137..5153)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5150..5172)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5161..5177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5185..5191,5192..5209,5210..5215))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5230..5251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5368..5385)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5539..6127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5619..5638)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6298..7089,7090..7158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6921..6940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7159..7263)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctgagc atcaacaagt ttgtacaaaa aagcaggctc caccatggat tacaaggacg
      961 atgacgacaa actcggatcc atgggaacca attcagtaga catgctagcc tgcctgaccc
     1021 gagggaactt actggacgtc ctgcaggagg gcttcaatga gcaacagctg caggcctatg
     1081 tggcctgggt gaatgcacag ctgaagaaga ggccagcagt gaagcctgtg caggacctgc
     1141 gacaagatct ccgggatggg gtgatcctgg catatctcat cgagattgtt gcaggagaaa
     1201 agctgagtgg ggtacagctg agtcccggta accaacagga gatgaagaat aatgtggaga
     1261 aagtgctaca gtttgtggcc tctaaaaaga ttcgtatgca ccagacttcg gctaaagata
     1321 ttgtggatgg aaacctgaag tctatcatga ggctggtcct tgccttggca gctcatttca
     1381 aacctggctc cagcaggacg gtgaaccaag gacgggactc cagagcccct ctgcaaagtc
     1441 accgaccaca ctgtgccact gctgttgccc agggagcagc tgctgctctg gccgatgtgt
     1501 gtcatgacat gtcccgatca ggacgggatg tctttcgata tagacagagg aacagcagca
     1561 tggatgagga aattgagaat ccatactgga gtgtgcgggc cctagtgcag cagtacgaag
     1621 ggcaacaaag gtccccgtct gaatccagct gctccagcct gacttcaccc agtccaatcc
     1681 acagtgcaaa gagcgagtcc attataaccc agtcagaaga gaaggcagat tttgtgatta
     1741 ttcccgctga aggaatagag aacagaacag aagggacaga ttctccatta tctcgagact
     1801 ggcggccagg gagccctgga acctatctgg agacctcatg ggaagaacag ctgttggaac
     1861 aacaagaata tttagaaaaa gaaatggagg aagcaaagaa aatgatatca ggactacagg
     1921 ccttactgct caatggatcc ttacctgaag atgaacagga gaggcccttg gccctctgtg
     1981 aaccaggtgt caatcccgag gaacaactga ttataatcca aagtcgtctg gatcagagta
     2041 tggaggagaa tcaggactta aagaaggaac tgctgaaatg taaacaagaa gccagaaact
     2101 tacaggggat aaaggatgcc ttgcagcaga gattgactca gcaggacaca tctgttcttc
     2161 agctcaaaca agagctactg agggcaaata tggacaaaga tgagctgcac aaccagaatg
     2221 tggatctgca gaggaagcta gatgagagga accggctctt gggagaatat aaaaaagagc
     2281 tggggcagaa ggatcgcctt cttcagcagc accaggccaa gttagaagaa gcactccgga
     2341 aactctctga tgtcagttac caccaggtgg atctagagcg agagctagaa cacaaagatg
     2401 tcctcttggc tcactgtatg aaaagagagg cagatgaggc gaccaactac aacagtcaca
     2461 actctcaaag caatggtttt ctccttccaa cggcaggaaa aggagctact tcagtcagca
     2521 acagagggac cagcgacctg cagcttgttc gagatgctct ccgcagcctg cgcaacagct
     2581 tcagtggcca cgatcctcag caccacacta ttgacagctt ggagcagggc atttctagcc
     2641 tcatggagcg cctgcatgtt atggagacgc agaagaaaca agaaagaaag gttcgggtca
     2701 agtcacccag aactcaagta ggtagtgaat accgggagtc ctggccccct aactcaaagt
     2761 tgcctcactc acagagctct ccaactgtca gcagcacctg tactaaagtg ctctatttca
     2821 ctgaccggtc acttacgccc ttcatggtca atataccaaa gaggttggag gaggtgacgt
     2881 taaaggattt taaagcagct attgatcggg aaggaaatca ccggtatcac ttcaaagcac
     2941 tggatcctga gtttggcact gtcaaagagg agattttcca tgatgatgat gccatccctg
     3001 gatgggaagg gaaaattgta gcttgggtgg aagaagacca tggagagaat taagcggccg
     3061 cactcgagat atctagaccc agctttcttg tacaaagtgg ttgatgctgt taacgggaag
     3121 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtgctgg tggctgttgt
     3181 cctggctgtt gcggtggcgg ctagtaatga gtttaaacgg gggaggctaa ctgaaacacg
     3241 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     3301 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
     3361 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
     3421 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
     3481 ctgccatagc agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3541 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3601 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3661 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3721 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3781 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3841 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3901 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3961 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4021 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4081 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4141 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4201 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4261 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     4321 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     4381 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc
     4441 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     4501 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     4561 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     4621 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     4681 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     4741 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     4801 gagggctaag cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     4861 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4921 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     4981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     5101 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     5161 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     5221 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     5281 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     5341 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     5401 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     5461 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     5521 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5581 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5641 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5701 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5761 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5821 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5881 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5941 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     6001 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6061 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6121 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     6181 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     6241 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     6301 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     6361 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     6421 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     6481 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6541 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6601 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6661 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6721 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6781 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6841 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6901 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6961 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7021 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7081 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7141 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     7201 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     7261 gcgcacattt ccccgaaaag tgccacctga cgtc
//
