LOCUS       sequence-475782-       12622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning gene libraries from oligonucleotide pools.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Dvir G, Xing Z, Beldman I, Rivera A, Wheeldon I, Cutler SR
  TITLE     Synthesis of large single-transcript pathways from oligonucleotide 
            pools: Design of STARBURST, an autobioluminescent reporter.
  JOURNAL   Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2508109122. doi: 
            10.1073/pnas.2508109122. Epub 2025 Jul 29.
  PUBMED    40729380
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2025-08-5"; volume: "122(31):e2508109122. 
            doi"; pages: " 10.1073/pnas.2508109122. Epub 2025 Jul 29"
COMMENT     Sequence Label: pPlantPOP
FEATURES             Location/Qualifiers
     source          1..12622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    188..212
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(258..510)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(550..1344)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(661..680)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1271..1290
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1378..1557)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2458..2492
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             2501..2512
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      3192..3263
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3279..3306
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4559..4903
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4847..4867
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4977..5654
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5157..5177)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5569..5592
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      5678..5930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5965..5983)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6172..6196
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7497..8126
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8560..9627
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      9693..9887
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10123..10145)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10231..10371
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10286..10305
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10386..10403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10557..11145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10637..10656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11233..12024)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
       61 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
      121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
      181 tggctggtgg caggatatat tgtggtgtaa acataacgga tccggtctca ggagagcgat
      241 cagcttgcat gccggtcgat ctagtaacat agatgacacc gcgcgcgata atttatccta
      301 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
      361 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      421 gtaattcaac agaaattata tgataatcat ggcaagaccg gcaacaggat tcaatcttaa
      481 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc tagctagagt ccgaacccca
      541 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gctatgcgct gcgaatcggg
      601 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
      661 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
      721 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcgtcgcc
      781 gtgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg cgaacagttc
      841 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
      901 catccgagta cgtgctcgct cgatgcggtg tttcgcttgg tggtcgaatg ggcaggtagc
      961 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     1021 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     1081 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     1141 cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt cggtcttgac
     1201 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     1261 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     1321 gtgcaatcca tcttgttcaa tcatgcctcg atcgagttga gagtgaatat gagactctaa
     1381 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     1441 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     1501 ttagctcatt aaactccaga aacccgcggc tcagtggctc cttcaacgtt gcggttctgt
     1561 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa ccgtgactcc
     1621 cttaattctc atgtatctcc gtcaggagtc tcaatggtaa ctttactctt tatttaacca
     1681 tacatttttt tttatttttt tcactttgtt cttcatccac tattgttctt tgttcatctt
     1741 gaacaaaagc tccctccttc tttgttcttc atccaccatt gttcttcatc aatcatttcg
     1801 ctgtcaggag gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
     1861 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
     1921 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
     1981 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
     2041 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
     2101 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
     2161 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
     2221 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
     2281 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
     2341 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
     2401 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagaatg agagacgttt
     2461 acggctagct cagtcctagg tatagtgcta gctactagag aaagaggaga aatactaaat
     2521 gagtgtgatc gctaaacaaa tgacctacaa ggtttatatg tcaggcacgg tcaatggaca
     2581 ctactttgag gtcgaaggcg atggaaaagg taagccctac gagggggagc agacggtaaa
     2641 gctcactgtc accaagggcg gacctctgcc atttgcttgg gatattttat caccacagtg
     2701 tcagtacgga agcataccat tcaccaagta ccctgaggac atccctgact atgtaaagca
     2761 gtcattcccg gagggctata catgggagag gatcatgaac tttgaagatg gtgcagtgtg
     2821 tactgtcagc aatgattcca gcatccaagg caactgtttc atctaccatg tcaagttctc
     2881 tggtttgaac tttcctccca atggacctgt catgcagaag aaaacacagg gctgggaacc
     2941 caacactgag cgtctgtttg cacgagatgg aatgctgcta ggaaacaact ttatggctct
     3001 gaagttagaa ggaggcggtc actatttgtg tgaatttaaa actacttaca aggcaaagaa
     3061 gcctgtgaag atgccagggt atcactatgt tgaccgcaaa ctggatgtaa ccaatcacaa
     3121 caaggattac acttcggttg agcagtgtga aatttccatt gcacgcaaac ctgtggtcgc
     3181 ctaataagct tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3241 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     3301 tctgcgcgtc tctgcttagc tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt
     3361 caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt attatggcat
     3421 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
     3481 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
     3541 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
     3601 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg
     3661 gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac cattatgctt
     3721 attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt actgaataca
     3781 agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt ttccagaatc
     3841 cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt tgtagttgag
     3901 tatgaaaata ttttttaatg cattttatga cttgccgctg tcaggagact agagccaagc
     3961 tgatctcctt tgccccggag atcaccatgg acgactttct ctatctctac gatctaggaa
     4021 gaaagttcga cggagaaggt gacgatacca tgttcaccac cgataatgag aagattagcc
     4081 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcggcaggtc
     4141 tgatcaagac gatctacccg agtaataatc tccaggagat caaatacctt cccaagaagg
     4201 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     4261 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     4321 ggcaagtaat agagattgga gtctctaaga aagtagttcc tactgaatca aaggccatgg
     4381 agtcaaaaat tcagatcgag gatctaacag aactcgccgt gaagactggc gaacagttca
     4441 tacagagtct tttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     4501 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
     4561 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
     4621 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
     4681 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
     4741 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     4801 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     4861 acccttcctc tatataagga agttcatttc atttggagag gactccggta tttttacaac
     4921 aattaccaca acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaatgg
     4981 cctcctccga gaacgtcatc accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg
     5041 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggccaca
     5101 acaccgtgaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     5161 ccccccagtt ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact
     5221 acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     5281 gcggcgtggc gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg
     5341 tgaagttcat cggcgtgaac ttcccctccg acggccccgt gatgcagaag aagacgatgg
     5401 gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagacac
     5461 acaaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca
     5521 tggccaagaa gcccgtgcag ctgcccggct actactacgt ggacgccaag ctggacatca
     5581 cctcccacaa cgaggactac accatcgtgg agcagtacga gcgcaccgag ggccgccacc
     5641 acctgttcct gtaggcttgg aatggatctt cgatcccgat cgttcaaaca tttggcaata
     5701 aagtttctta agattgaatc ctgttgccgg tcttgcgacg attatcatat aatttctgtt
     5761 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     5821 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     5881 cgcaaactag gataaattat cgcgcdcggt gtcatctatg ttactagatc gggaattgcc
     5941 aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     6001 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6121 gataaatgct tcaataatgg gaccgactcg cgctgtcatg agaccggatc ctgacaggat
     6181 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     6241 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     6301 tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     6361 ttcagtgcag ccgtcatctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     6421 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     6481 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     6541 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     6601 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     6661 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     6721 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     6781 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     6841 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     6901 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     6961 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     7021 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     7081 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     7141 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     7201 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     7261 atcgcggccg ggtacgtgtt cgagccgccc gcgcacctct caaccgtgcg gctgcatgaa
     7321 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     7381 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     7441 gcggtcgctg cgtatatgat ccgatgagta aataaacaaa tacgcaaggg gaacgcatga
     7501 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc ggaacccatc
     7561 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     7621 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     7681 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     7741 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     7801 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     7861 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     7921 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     7981 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     8041 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     8101 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     8161 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     8221 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     8281 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     8341 gatcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     8401 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     8461 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
     8521 atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg
     8581 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     8641 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     8701 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     8761 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     8821 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     8881 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     8941 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     9001 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     9061 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     9121 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     9181 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     9241 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     9301 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     9361 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     9421 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     9481 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     9541 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     9601 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     9661 gcaggggaaa aaggtcgaaa aggactcttt cctgtggata gcacgtacat tgggaaccca
     9721 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     9781 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
     9841 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
     9901 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
     9961 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    10021 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    10081 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10141 cccggtgacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10201 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    10261 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10321 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    10381 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10981 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11221 cattctaggt gattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
    11281 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
    11341 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    11401 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    11461 aagcactaca tttcgctcat caccagccca gtcgggcggc gagttccata gcgttaaggt
    11521 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    11581 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    11641 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    11701 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    11761 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    11821 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    11881 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    11941 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    12001 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
    12061 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
    12121 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
    12181 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
    12241 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
    12301 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
    12361 atttctgtcc tggctggaac agaacttatt atttccttcc tcttttctac agtatttaaa
    12421 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    12481 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    12541 agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg
    12601 ttacaatcaa catgctaccc tc
//
