LOCUS       sequence-475787-        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology rbcL in the M1 position used for genome integration at 
            the rbcL locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_1_005_rbcL_5'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2168..2225
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2249..2266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2420..3008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2500..2519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3191..3285)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3306..3965)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3880..3899
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4127..4145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4149..4192)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4195..4216
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaac acgtctcgaa cagtaaaaac acataactcc acgtaagcgc attttcttac
       61 aatcaaagaa tgccaattgt tctttgcttg ttgtaaaaga aaaactaggc tttttctttg
      121 aaaacatttc agcatattca acaactttta tagattggtt tagcggatga tgggactcga
      181 acccacaacc tcgaacttgg aaggatcgca ctctaccgat tgagttacat ccgctttagt
      241 atgttactat ttcttttatt ataacttata aaatataata cataaagata aattctataa
      301 taaaaagcta agattttatt tttctggcac atcgtaattt ataaagacag gcaaatttaa
      361 acaaaagata actttagaac ttaattttaa aaatgtaaaa tgatgtttag gtatttaacc
      421 taaacaccat aaaaataaaa acgatgttta tgctattcac ataaacatca tgaaaaataa
      481 aaattaaagt ttgtcaatag tatcaaattc gaatttaatt tctttccaaa cttcacatgc
      541 agcagcaagt tctggtgacc atttacaagc tgaacgaatt acgtcgccac cttcacgagc
      601 aaggtcacga ccttcgttac gagcttgagt acaagcttca agagctacac ggttagctgc
      661 agcacctgga gcgttacccc aagggtgacc tagagtacca ccaccgaact gaagacatgc
      721 gtcatcaccg aagatttcaa ctaaagctgg catgtgccat acgtgaatac cacctgaagc
      781 aactggcata acacctggca ttgaacacca gtcttgagtg aagtaaatac cacggctacg
      841 gtctttttca acgtagtcat cacgcattaa gtctacgaaa cctagagtaa cttcacgttc
      901 accttctagt ttacctacaa cagtaccaga gtgaaggtgg tcaccaccag acatacgaag
      961 agctttagca agaacacgga agtgaatacc gtggttacgt tgacggtcaa taaccgcgtg
     1021 catagcacgg tggatgtgta gaagaagacc gttgtcacga cagtagatag ctaatgaagt
     1081 gttagctgtg aaaccacctg ttaagtagtc gtgcataata ataggtacac ctaattcttt
     1141 agcacatact gcacgtttca tcatttcttc acaagtacca gcagtagcgt ttaagtagtg
     1201 acctttaact tcacctgttt ctgcttgagc tttgtaaata gcttcagcaa cgaaaaggaa
     1261 acggtcacgc caacgcatga atggttgtga gtttacgttt tcgtcgtctt tagtaaagtc
     1321 aagaccacca cgtaaacatt cataaactgc acgaccgtag tttttagctg aaagacctaa
     1381 tttaggtttg attgtacaac ctaaaagacc acgaccatat ttgtttaatt tgtcacgttc
     1441 tacctgaata ccgtgtggag gacctacgaa tgttttaacg taagcaggtg gaatacgaag
     1501 gtcttcaaga cgtagagcac gtaaagcttt gaaaccgaat acgttaccta caatagaagt
     1561 gaacatgtta gttactgaac cttcttcgaa taagtcgatt gggtaagcta cgtaagcaat
     1621 gtattggttg tcttcacccg gaactggttc gatatcgtaa caacgacctt tgtaacggtc
     1681 aagacttgtt aaaccgtcag tccatactgt agtccatgta cctgttgaag attcagcagc
     1741 tacagcagca ccacattctt caggtggaac acctggttgt ggagtcatac ggaatgcagc
     1801 taaaatatca gtatctctta ctacgtaatc aggtgtgtag tatgttaaac ggtagtcttt
     1861 tacaccggct ttgaatccag cacctgcttt agtttctgtt tgtggaacca tttatataaa
     1921 taaatgtaac ttcttttgac gatcctaaaa taatctgtcc ggaaatataa tttaaaaatt
     1981 gttgaaaaat tttaaataca tttaaatata ttatatattt agaaataaaa aataatgtaa
     2041 actagcaaat ttatgtactt gtcttatcag tcgagcatag aaagggcctt taggtatatg
     2101 tcggtcgtct tacgcagttt acttacggag tgagaccact catcgcgact agtagcggcc
     2161 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     2221 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     3181 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     3241 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     3301 tcaaattacg ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg
     3361 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     3421 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     3481 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac
     3541 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     3601 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa
     3661 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     3721 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     3781 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     3841 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     3901 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     3961 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     4021 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     4081 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4141 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     4201 gcggccgctt ctagagcgat gagt
//
