LOCUS       sequence-475789-        4446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Homology psbD in the M1 position used for genome integration at 
            the psbD locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_1_010_psbD_5'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..4446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2390..2447
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2471..2488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2642..3230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2722..2741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3413..3507)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3528..4187)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4102..4121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     4349..4367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4371..4414)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    4417..4438
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaac acgtctcgaa caggtttgga ataataaaaa tcgaaaaaaa gaattcactc
       61 aaagaaaaaa acgtttgact ttaatttcgg ataaactgca taaaatttat agtttctaca
      121 aagtttatct agtttaggga tataaatata gagcaaatca attgaattgc tatacccaaa
      181 gaggcagaga tgtccttaaa aataaattaa agttttttca gacctagctg atttaataca
      241 ttttgtgttc tattagactt gttaatgtat tttttttgag tatccttaat aatattaaaa
      301 tattattttt acacgttaca tatattagta ttttatttta acgataaacg ctttgtggtt
      361 attaaaatgg atttacctct gttaggctat ggaacttgat aaaaccgtcc cttaagggga
      421 tgtcttacaa ggatttagga tctgccattt gtattaagag tatagatttt aataaaaaac
      481 caaaagattt ttaaaaaagt ggattgtaaa aatcttttta tatgataaac cacttctgtc
      541 tttttagaca taattgaaac taaaaagtaa gtttttctga aaaaataaaa agaactaaag
      601 caaaatgcaa ataattgcct tatactgctt tgatttttaa aagtgcttat acttttcaac
      661 tacagtgtaa aagtatcttt ttaaactata aaaatatata cgtcttcgtg tcgcacccac
      721 ttaaccaaat gaaaatgata cctaattgac caaagtgtgc actgaatact tttctagaaa
      781 tttcttctag atcacttgta tgactgtcaa agtcatgagc atctgcgtga aggttccaaa
      841 tccaagttgt tgtgtttgga ccttttgaca gagtacgtga aaaatgtcct ggtttagtca
      901 ggtaggattt gtttttttat tttttaatat tttaaatcct cctaaagaac cgtacatgcg
      961 agttttttcg catacggctc aagcactcat gtgattttta gccccaaagg ggcattttct
     1021 tttaaataat aaagtaatat aaattaccaa tactaattag tattggtaat ttatataaaa
     1081 atatattata gagcgttacc acgtggtaat acttcttcag ggaatactaa acgttcgtgt
     1141 ggttggtctt gagcagccat ccaagcacga ataccttcgt taagaagaat gtttttagtg
     1201 tagaatgttt cgaattcagg gtcttcagca gcacgaatct cttgtgatac gaagtcgtaa
     1261 gcacgtaagt ttagagctaa acctacaaca ccaatagcac tcatccaaag accagttact
     1321 ggaactaata acatgaagaa gtgaagccaa cgtttgttag agaaagcaac accgaagatt
     1381 tgtgaccaga aacggttagc agtaaccata gagtatgttt cttcagcctg tgtagggttg
     1441 aatgcacgga atgtgttagc accgtcaccg tcttcgaata atgtgttttc aacagtagca
     1501 ccgtgaatag cacataataa agcagcacct aaaacaccag caacacccat catgtggaat
     1561 gggttaagtg tccagttgtg gaaaccttgg aagaataaaa tgaaacggaa gatagcagct
     1621 acaccgaaac taggtgcaaa gaaccaacct gattgaccta atgggtaaat taggaatact
     1681 gaaacgaata cagcaattgg tgctgagaaa gcaattgcgt tgtatggacg taagtttact
     1741 gaacgagcaa tttcaaactg acgaagcatg aaaccaatta aaccaaatgc accgtgtaaa
     1801 gcaacgaatg cccataaacc accaagttga caccaacgag tgaaatcacc ttgagcttct
     1861 ggaccccaaa caaatagaag agagtgagcc atactgttag caggtgtaga aacagctgct
     1921 gttaagaagt tacaaccttc taagtaagaa gtagctaaac catgcgtata ccatgaagta
     1981 acgaaagtag taccagttaa ccaaccacct aatgcaaagt aagcacaagg gaatagtaat
     2041 aaacctgacc aacctacgaa tacgaaacgg tcttgacgaa gccagtcatc agcgtcatcg
     2101 aaccatgtgc gtttctcttg atatgtaccg atcgcaattg tcattgcgtg tatctccaaa
     2161 ataaaaaaac aactcatcgt tacgttaaat ttattattat ttaattttaa tcattgtgta
     2221 tttaatatta taacttatat aaaataaaat taaaaataag cattttttac acacatattt
     2281 ttaaataaat ctttaaacgg gttatatata gttatatata tgggactaga actgctttgt
     2341 gcatagtcgg agtgagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa
     2401 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     2461 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3061 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     3421 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     3481 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     3541 tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     3601 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     3661 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     3721 aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct
     3781 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     3841 aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca
     3901 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     3961 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     4021 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     4081 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     4141 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     4201 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     4261 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     4321 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     4381 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg
     4441 atgagt
//
