LOCUS       sequence-47579-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: Lyn-mCherry-GAI(1-92)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(260..278)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     528..547
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             848..865
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tccggccacc
       61 atgggatgta taaaatcaaa agggaaagac agcgcggatc caccggtcgc caccatggta
      121 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      181 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      241 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      301 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      361 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      421 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      481 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      541 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      601 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      661 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
      721 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgccgc
      781 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagagcgcag gaggaatgaa
      841 gagagatcat catcatcatc atcatcagat agagac
//
