LOCUS       sequence-47581-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: Tom20-mCherry-GAI(1-92)
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(303..321)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     571..590
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagatccgct agcgcttacc ggntgaattc gccaccatgg ntgggtcgga acagcgccat
       61 cgccgcgggc gtgtgcggtg ccctcttcat agggtactgc atctactttg accgcaaaag
      121 acgaagtgac cccaacttcg gatccggagc aagtggaatg gtgagcaagg gcgaggagga
      181 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      241 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      301 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      361 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      421 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      481 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      541 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
      601 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      661 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      721 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
      781 ccacaacgag gactacacca tcgtggaac
//
