LOCUS       sequence-47582-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: NLS-GAI(1-92)
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..51
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             79..99
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             133..150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    406..471
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    595..716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(632..651)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     686..705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc caccatggat ccaaaaaaga agagaaaggt agatccaaaa
       61 aagaagagaa aggtagatcc aaaaaagaag agaaaggtac tgcagtcacc ggtcgccacc
      121 atgaagagag atcatcatca tcatcatcat caagataaga agactatgat gatgaatgaa
      181 gaagacgacg gtaacggcat ggatgagctt ctagctgttc ttggttacaa ggttaggtca
      241 tccgaaatgg ctgatgttgc tcagaaactc gagcagcttg aagttatgat gtctaatgtt
      301 caagaagacg atctttctca actcgctact gagactgttc actataatcc ggcggagctt
      361 tacacgtggc ttgattctat gctcaccgac cttaatctgt acaagtccgg actcagatct
      421 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
      481 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      541 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
      721 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
      781 attttttaac caataggccg aaatcggcaa aatccct
//
