LOCUS       sequence-475867-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPSG803
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        284..359
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        536..723
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             724..1386
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     misc_feature    1601..2104
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(2148..2963)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2148..2963)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2871..2890
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2955..2974
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3188..3776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3677..3696
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4638..8741
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4638..8741
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8754..8774
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8778..8798
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atgtgcttca gtattacatt ttttgccttc aacgccttga ttgttctatt tttgctaata
       61 ataaatctat ttcatcggac taaaagtcca ttagttgtaa gcggatttag ctcagttggg
      121 agagcgccag actgaagaaa aacttcggtc aagtcatctg gaggtcctgt gttcgatcca
      181 cagaattcgc agatggccgg catggtccca gcctcctcgc tggcgccggc tgggcaacac
      241 cttcgggtgg cgaatgggac tttagagaga agttacataa cgagttttag agctagaaat
      301 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
      361 ttttttattt tttgtcacta ttgttatgta aaatgccacc tctgacagta tggaacgcaa
      421 acttctgtct agtggatagc tgagcagtta cagagatgtt acgaaccact agtgcactgc
      481 agtacaaaca cagtcctttc ccgcaatttt ctttttctat tactcttggc ctcctctagt
      541 acactctata tttttttatg cctcggtaat gattttcatt tttttttttc cacctagcgg
      601 atgactcttt ttttttctta gcgattggca ttatcacata atgaattata cattatataa
      661 agtaatgtga tttcttcgaa gaatatacta aaaaatgagc aggcaagata aacgaaggca
      721 aagatgacag agcagaaagc cctagtaaag cgtattacaa atgaaaccaa gattcagatt
      781 gcgatctctt taaagggtgg tcccctagcg atagagcact cgatcttccc agaaaaagag
      841 gcagaagcag tagcagaaca ggccacacaa tcgcaagtga ttaacgtcca cacaggtata
      901 gggtttctgg accatatgat acatgctctg gccaagcatt ccggctggtc gctaatcgtt
      961 gagtgcattg gtgacttaca catagacgac catcacacca ctgaagactg cgggattgct
     1021 ctcggtcaag cttttaaaga ggccctaggg gccgtgcgtg gagtaaaaag gtttggatca
     1081 ggatttgcgc ctttggatga ggcactttcc agagcggtgg ttgatctttc gaacaggccg
     1141 tacgcagttg tcgaacttgg tttgcaaagg gagaaagtag gtgatctctc ttgcgagatg
     1201 atcccgcatt ttcttgaaag ctttgcagag gctagcagaa ttaccctcca cgttgattgt
     1261 ctgcgaggca agaatgatca tcaccgtagt gagagtgcgt tcaaggctct tgcggttgcc
     1321 ataagagaag ccacctcgcc caatggtacc aacgatgttc cctccaccaa aggtgttctt
     1381 atgtagtgac accgattatt taaagctgct gcatacgata tatatacatg tgtatatatg
     1441 tatacctatg aatgtcagta agtatgtata cgaacagtat gatactgaag atgacaaggt
     1501 aatgcatcat tctatacgtg tcattctgaa cgaggcgcgc tttccttttt tctttttgct
     1561 ttttcttttt ttttctcttg aactcgacgg atcatagagt atcacgtgct ataaaaataa
     1621 ttataattta aattttttaa tataaatata taaattaaaa atagaaagta aaaaaagaaa
     1681 ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag actctagggg gatcgccaac
     1741 aaatactacc ttttatcttg ctcttcctgc tctcaggtat taatgccgaa ttgtttcatc
     1801 ttgtctgtgt agaaaaccac acacgaaaat cctgtgattt tacattttac ttatcgttaa
     1861 tcgaatgtat atctatttaa tctgcttttc ttgtctaata aatatatatg taaagtacgc
     1921 tttttgttga aattttttaa acctttgttt attttttttt cttcattccg taactcttct
     1981 accttcttta tttactttct aaaatccaaa tacaaaacat aaaaataaat aaacacagag
     2041 taaattccca aattattcca tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc
     2101 gatccgtccc gagcggccgc gtgttacaac caattaacca attctgatta gaaaaactca
     2161 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     2221 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2281 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2341 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2401 aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2461 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagg
     2521 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2581 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2641 tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2701 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2761 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2821 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2881 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct ggagcaagac
     2941 gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt
     3001 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac
     3061 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gtcatgacca
     3121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3841 ttcctgcgtt atcccctgat tctgtggata accgtgcggc cgcccctgaa ttcgcatcta
     3901 gactgaactg gccgataatt gcagacgaac ggtgagtaag gaaagagtga ggaactatcg
     3961 catacctgca tttaaagatg ccgatttggg cgcgaatcct ttattttggc ttcaccctca
     4021 tactattatc agggccagaa aaaggaagtg tttccctcct tcttgaattg atgttaccct
     4081 cataaagcac gtggcctctt atcgagaaag aaattaccgt cgctcgtgat ttgtttgcaa
     4141 aaagaacaaa actgaaaaaa cccagacacg ctcgacttcc tgtcatccta ttgattgcag
     4201 cttccaattt cgtcacacaa caaggtccta gcgacggctc acaggttttg taacaagcaa
     4261 tcgaaggttc tggaatggcg ggaaagggtt tagtaccaca tgctatgatg cccactgtga
     4321 tctccagagc aaagttcgtt cgatcgtact gttactctct ctctttcaaa cagaattgtc
     4381 cgaatcgtgt gacaacaaca gcctgttctc acacactctt ttcttctaac caagggggtg
     4441 gtttagttta gtagaacctc gtgaaactta catttacata tatataaact tgcataaatt
     4501 ggtcaatgca agaaatacat atttggtctt ttctaattcg tagtttttca agttcttaga
     4561 tgctttcttt ttctcttttt tacagatcat caaggaagta attatctact ttttacaaca
     4621 aatataaaac aagatctatg gacaagaagt attctatcgg actggatatc gggactaata
     4681 gcgtcgggtg ggccgtgatc actgacgagt acaaggtgcc ctctaagaag ttcaaggtgc
     4741 tcgggaacac cgaccggcat tccatcaaga aaaatctgat cggagctctc ctctttgatt
     4801 caggggaaac cgctgaagca acccgcctca agcggactgc tagacggcgg tacaccagga
     4861 ggaagaaccg gatttgttac cttcaagaga tattctccaa cgaaatggca aaggtcgacg
     4921 acagcttctt ccataggctg gaagaatcat tcctcgtgga agaggataag aagcatgaac
     4981 ggcatcccat cttcggtaat atcgtcgacg aggtggccta tcacgagaaa tacccaacca
     5041 tctaccatct tcgcaaaaag ctggtggact caaccgacaa ggcagacctc cggcttatct
     5101 acctggccct ggcccacatg atcaagttca gaggccactt cctgatcgag ggcgacctca
     5161 atcctgacaa tagcgatgtg gataaactgt tcatccagct ggtgcagact tacaaccagc
     5221 tctttgaaga gaaccccatc aatgcaagcg gagtcgatgc caaggccatt ctgtcagccc
     5281 ggctgtcaaa gagccgcaga cttgagaatc ttatcgctca gctgccgggt gaaaagaaaa
     5341 atggactgtt cgggaacctg attgctcttt cacttgggct gactcccaat ttcaagtcta
     5401 atttcgacct ggcagaggat gccaagctgc aactgtccaa ggacacctat gatgacgatc
     5461 tcgacaacct cctggcccag atcggtgacc aatacgccga ccttttcctt gctgctaaga
     5521 atctttctga cgccatcctg ctgtctgaca ttctccgcgt gaacactgaa atcaccaagg
     5581 cccctctttc agcttcaatg attaagcggt atgatgagca ccaccaggac ctgaccctgc
     5641 ttaaggcact cgtccggcag cagcttccgg agaagtacaa ggaaatcttc tttgaccagt
     5701 caaagaatgg atacgccggc tacatcgacg gaggtgcctc ccaagaggaa ttttataagt
     5761 ttatcaaacc tatccttgag aagatggacg gcaccgaaga gctcctcgtg aaactgaatc
     5821 gggaggatct gctgcggaag cagcgcactt tcgacaatgg gagcattccc caccagatcc
     5881 atcttgggga gcttcacgcc atccttcggc gccaagagga cttctacccc tttcttaagg
     5941 acaacaggga gaagattgag aaaattctca ctttccgcat cccctactac gtgggacccc
     6001 tcgccagagg aaatagccgg tttgcttgga tgaccagaaa gtcagaagaa actatcactc
     6061 cctggaactt cgaagaggtg gtggacaagg gagccagcgc tcagtcattc atcgaacgga
     6121 tgactaactt cgataagaac ctccccaatg agaaggtcct gccgaaacat tccctgctct
     6181 acgagtactt taccgtgtac aacgagctga ccaaggtgaa atatgtcacc gaagggatga
     6241 ggaagcccgc attcctgtca ggcgaacaaa agaaggcaat tgtggacctt ctgttcaaga
     6301 ccaatagaaa ggtgaccgtg aagcagctga aggaggacta tttcaagaaa attgaatgct
     6361 tcgactctgt ggagattagc ggggtcgaag atcggttcaa cgcaagcctg ggtacttacc
     6421 atgatctgct taagatcatc aaggacaagg attttctgga caatgaggag aacgaggaca
     6481 tccttgagga cattgtcctg actctcactc tgttcgagga ccgggaaatg atcgaggaga
     6541 ggcttaagac ctacgcccat ctgttcgacg ataaagtgat gaagcaactt aaacggagaa
     6601 gatataccgg atggggacgc cttagccgca aactcatcaa cggaatccgg gacaaacaga
     6661 gcggaaagac cattcttgat ttccttaaga gcgacggatt cgctaatcgc aacttcatgc
     6721 aacttatcca tgatgattcc ctgaccttta aggaggacat ccagaaggcc caagtgtctg
     6781 gacaaggtga ctcactgcac gagcatatcg caaatctggc tggttcaccc gctattaaga
     6841 agggtattct ccagaccgtg aaagtcgtgg acgagctggt caaggtgatg ggtcgccata
     6901 aaccagagaa cattgtcatc gagatggcca gggaaaacca gactacccag aagggacaga
     6961 agaacagcag ggagcggatg aaaagaattg aggaagggat taaggagctc gggtcacaga
     7021 tccttaaaga gcacccggtg gaaaacaccc agcttcagaa tgagaagctc tatctgtact
     7081 accttcaaaa tggacgcgat atgtatgtgg accaagagct tgatatcaac aggctctcag
     7141 actacgacgt ggaccatatc gtccctcaga gcttcctcaa agacgactca attgacaata
     7201 aggtgctgac tcgctcagac aagaaccggg gaaagtcaga taacgtgccc tcagaggaag
     7261 tcgtgaaaaa gatgaagaac tattggcgcc agcttctgaa cgcaaagctg atcactcagc
     7321 ggaagttcga caatctcact aaggctgaga ggggcggact gagcgaactg gacaaagcag
     7381 gattcattaa acggcaactt gtggagactc ggcagattac taaacatgtc gcccaaatcc
     7441 ttgactcacg catgaatacc aagtacgacg aaaacgacaa acttatccgc gaggtgaagg
     7501 tgattaccct gaagtccaag ctggtcagcg atttcagaaa ggactttcaa ttctacaaag
     7561 tgcgggagat caataactat catcatgctc atgacgcata tctgaatgcc gtggtgggaa
     7621 ccgccctgat caagaagtac ccaaagctgg aaagcgagtt cgtgtacgga gactacaagg
     7681 tctacgacgt gcgcaagatg attgccaaat ctgagcagga gatcggaaag gccaccgcaa
     7741 agtacttctt ctacagcaac atcatgaatt tcttcaagac cgaaatcacc cttgcaaacg
     7801 gtgagatccg gaagaggccg ctcatcgaga ctaatgggga gactggcgaa atcgtgtggg
     7861 acaagggcag agatttcgct accgtgcgca aagtgctttc tatgcctcaa gtgaacatcg
     7921 tgaagaaaac cgaggtgcaa accggaggct tttctaagga atcaatcctc cccaagcgca
     7981 actccgacaa gctcattgca aggaagaagg attgggaccc taagaagtac ggcggattcg
     8041 attcaccaac tgtggcttat tctgtcctgg tcgtggctaa ggtggaaaaa ggaaagtcta
     8101 agaagctcaa gagcgtgaag gaactgctgg gtatcaccat tatggagcgc agctccttcg
     8161 agaagaaccc aattgacttt ctcgaagcca aaggttacaa ggaagtcaag aaggacctta
     8221 tcatcaagct cccaaagtat agcctgttcg aactggagaa tgggcggaag cggatgctcg
     8281 cctccgctgg cgaacttcag aagggtaatg agctggctct cccctccaag tacgtgaatt
     8341 tcctctacct tgcaagccat tacgagaagc tgaaggggag ccccgaggac aacgagcaaa
     8401 agcaactgtt tgtggagcag cataagcatt atctggacga gatcattgag cagatttccg
     8461 agttttctaa acgcgtcatt ctcgctgatg ccaacctcga taaagtcctt agcgcataca
     8521 ataagcacag agacaaacca attcgggagc aggctgagaa tatcatccac ctgttcaccc
     8581 tcaccaatct tggtgcccct gccgcattca agtacttcga caccaccatc gaccggaaac
     8641 gctatacctc caccaaagaa gtgctggacg ccaccctcat ccaccagagc atcaccggac
     8701 tttacgaaac tcggattgac ctctcacagc tcggagggga tgagggagct gatccaaaaa
     8761 agaagagaaa ggtagatcca aaaaagaaga gaaaggtata aggatcctaa ctcgagattg
     8821 aattgaattg aaatcgatag atcaattttt ttcttttctc tttccccatc ctttacgcta
     8881 aaataatagt ttattttatt ttttgaatat tttttattta tatacgtata tatagactat
     8941 tatttatctt ttaatgatta ttaagatttt tattaaaaaa aaattcgctc ctcttttaat
     9001 gcctttatgc agtttttttt tcccattcga tatttctatg tgctgccaaa ccagatgtca
     9061 acacagctac aacg
//
