LOCUS       sequence-475871-        7867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Idi1p and Erg20WW for synthesis of GPP in the peroxisome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7867)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJJB1434
FEATURES             Location/Qualifiers
     source          1..7867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4140..4943
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(5585..6400)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5585..6400)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6308..6327
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6392..6411
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6625..7213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7114..7133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgtgtgg agtgtttgct tggattcttt agtaaaaggg
       61 gaagaacagt tggaagggcc aaagtggaag tcacaaaaca gtggtcctat ataaaagaac
      121 aagaaaaaga ttatttatat acaactgcgg tcacaagaag caacgcgaga gagcacaaca
      181 cgctgttatc acgcaaacta tgttttgaca ccgagccata gccgtgattg tgcgtcacat
      241 tgggcgataa tgaacgctaa atgaccaact cccatccgta ggagcccctt agggcgtgcc
      301 aatagtttca cgcgcttaat gcgaagtgct cggaacggac aactgtggtc gtttggcacc
      361 gggaaagtgg tactagaccg agagtttcgc atttgtatgg caggacgttc tgggagcttc
      421 gcgtctaaag ctttttcggg cgcgaaatgc agaccagacc agaacaaaac aactgacaag
      481 aaggcgttta atttaatatg ttgttcactc gcgcctgggc tgttgttatt cggctagata
      541 catacgtgtt tgtgcgtatg tagttatatc atatataagt atattaggat gaggcggtga
      601 aagagatttt ttttttttcg cttaatttat tcttttctct atcttttttc ctacatcttg
      661 ttcaaaagag tagcaaaaac aacaatcaat acaataaaat aagatctatg actgccgaca
      721 acaatagtat gccccatggt gcagtatcta gttacgccaa attagtgcaa aaccaaacac
      781 ctgaagacat tttggaagag tttcctgaaa ttattccatt acaacaaaga cctaataccc
      841 gatctagtga aacgtcaaat gacgaaagcg gagaaacatg tttttctggt catgatgagg
      901 agcaaattaa gttaatgaat gaaaattgta ttgttttgga ttgggacgat aatgctattg
      961 gtgccggtac caagaaagtt tgtcatttaa tggaaaatat tgaaaagggt ttactacatc
     1021 gtgcattctc cgtctttatt ttcaatgaac aaggtgaatt acttttacaa caaagagcca
     1081 ctgaaaaaat aactttccct gatctttgga ctaacacatg ctgctctcat ccactatgta
     1141 ttgatgacga attaggtttg aagggtaagc tagacgataa gattaagggc gctattactg
     1201 cggcggtgag aaaactagat catgaattag gtattccaga agatgaaact aagacaaggg
     1261 gtaagtttca ctttttaaac agaatccatt acatggcacc aagcaatgaa ccatggggtg
     1321 aacatgaaat tgattacatc ctattttata agatcaacgc taaagaaaac ttgactgtca
     1381 acccaaacgt caatgaagtt agagacttca aatgggtttc accaaatgat ttgaaaacta
     1441 tgtttgctga cccaagttac aagtttacgc cttggtttaa gattatttgc gagaattact
     1501 tattcaactg gtgggagcaa ttagatgacc tttctgaagt ggaaaatgac aggcaaattc
     1561 atagaatgct aggatccttg ggaagaggta gaagatccaa attgtaataa ctcgagtggc
     1621 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
     1681 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
     1741 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
     1801 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgc caaaccagat
     1861 gtcaacacag ctacaacgca cccatgaacc acacggttag tccaaaaggg gcagttcaga
     1921 ttccagatgc gggaattagc ttgctgccac cctcacctca ctaacgctgc ggtgtgcgga
     1981 tacttcatgc tatttataga cgcgcgtgtc ggaatcagca cgcgcaagaa ccaaatggga
     2041 aaatcggaat gggtccagaa ctgctttgag tgctggctat tggcgtctga tttccgtttt
     2101 gggaatcctt tgccgcgcgc ccctctcaaa actccgcaca agtcccagaa agcgggaaag
     2161 aaataaaacg ccaccaaaaa aaaaaaaata aaagccaatc ctcgaagcgt gggtggtagg
     2221 ccctggatta tcccgtacaa gtatttctca ggagtaaaaa aaccgtttgt tttggaattt
     2281 cccatttcgc ggccacctac gccgctatct ttgcaacaac tatctgcgat aactcagcaa
     2341 attttgcata ttcgtgttgc agtattgcga taatgggagt cttacttcca acataacggc
     2401 agaaagaaat gtgagaaaat tttgcatcct ttgcctccgt tcaagtatat aaagtcggca
     2461 tgcttgataa tctttctttc catcctacat tgttctaatt attcttattc tcctttattc
     2521 tttcctaaca taccaagaaa ttaatcttct gtcattcgct taaacactat atcaataaag
     2581 atctatggct tcagaaaaag aaattaggag agagagattc ttgaacgttt tccctaaatt
     2641 agtagaggaa ttgaacgcat cgcttttggc ttacggtatg cctaaggaag catgtgactg
     2701 gtatgcccac tcattgaact acaacactcc aggcggtaag ctaaatagag gtttgtccgt
     2761 tgtggacacg tatgctattc tctccaacaa gaccgttgaa caattggggc aagaagaata
     2821 cgaaaaggtt gccattctag gttggtgcat tgagttgttg caggcttact ggttggtcgc
     2881 cgatgatatg atggacaagt ccattaccag aagaggccaa ccatgttggt acaaggttcc
     2941 tgaagttggg gaaattgcca tctgggacgc attcatgtta gaggctgcta tctacaagct
     3001 tttgaaatct cacttcagaa acgaaaaata ctacatagat atcaccgaat tgttccatga
     3061 ggtcaccttc caaaccgaat tgggccaatt gatggactta atcactgcac ctgaagacaa
     3121 agtcgacttg agtaagttct ccctaaagaa gcactccttc atagttactt tcaagactgc
     3181 ttactattct ttctacttgc ctgtcgcatt ggccatgtac gttgccggta tcacggatga
     3241 aaaggatttg aaacaagcca gagatgtctt gattccattg ggtgaatact tccaaattca
     3301 agatgactac ttagactgct tcggtacccc agaacagatc ggtaagatcg gtacagatat
     3361 ccaagataac aaatgttctt gggtaatcaa caaggcattg gaacttgctt ccgcagaaca
     3421 aagaaagact ttagacgaaa attacggtaa gaaggactca gtcgcagaag ccaaatgcaa
     3481 aaagattttc aatgacttga aaattgaaca gctataccac gaatatgaag agtctattgc
     3541 caaggatttg aaggccaaaa tttctcaggt cgatgagtct cgtggcttca aagctgatgt
     3601 cttaactgcg ttcttgaaca aagtttacaa gagaagcaaa ggatccttgg gaagaggtag
     3661 aagatccaaa ttgtaataac tcgagtggcg ccaattggtg cggcaattga taataacgaa
     3721 aatgtctttt aatgatctgg gtataatgag gaattttccg aacgttttta ctttatatat
     3781 atatatacat gtaacatata ttctatacgc tatagagaaa ggaaattttt caattaaaaa
     3841 aaaaatagag aaagagtttc acttcttgat tatcgctaac actaatggtt gaagtactgc
     3901 tactttaatt ttatgctgag cagttacaga gatgttacga accactagtg cactgcagta
     3961 cacggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
     4021 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
     4081 gcccagtatt cttaacccaa ctgcacagaa caaaaacgtg caggaaacga agataaatca
     4141 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc
     4201 tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca
     4261 ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac
     4321 atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat
     4381 ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
     4441 tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg
     4501 cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa
     4561 caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta
     4621 ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg
     4681 gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga
     4741 cacccggtgt gggtttagat gacaagggag atgcattggg tcaacagtat agaaccgtgg
     4801 atgatgtggt ttctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
     4861 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
     4921 gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact
     4981 cacaaattag agcttcaatt taattatatc agttattacc cgagtagagc acttgaatcc
     5041 actgccccgg gaatctcggt cgtaatgatt tctataatga cgaaaaaaaa aaaattggaa
     5101 agaaaaagct tcatggcctt tataaaaagg aactatccaa tacctcgcca gaaccaagta
     5161 acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag atggacgcat
     5221 tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa atgaaggatt
     5281 tcatgcgttt gtactctaat ctggtagaaa gatgtttcac agactgtgtc aatgacttca
     5341 caacatcaaa gctaaccaat aaggaacaaa catgcatcat gaagtgctca gaaaagttct
     5401 tgaagcatag cgaacgtgta gggcagcgtt tccaagaaca aaacgctgcc ttgggacaag
     5461 gcttgggccg ataaggtgta ctggcgtata tatatctaat tatgtatctc tggtgtagcc
     5521 catttttagc atgtaaatat aaagaccgag cggccgcgtg ttacaaccaa ttaaccaatt
     5581 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     5641 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     5701 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     5761 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     5821 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     5881 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     5941 attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     6001 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     6061 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     6121 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     6181 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     6241 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     6301 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     6361 gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     6421 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     6481 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga
     6541 aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6601 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6661 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6721 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6781 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6841 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6901 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6961 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7021 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7081 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7141 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7201 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7261 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc
     7321 caatgctaaa ttcgagtgaa acacaggaag atcagaaaat cctcatttca tccatattaa
     7381 caataatttc aaatgtttat ttgcattatt tgaaactagg caagacaagc aacgaaacgt
     7441 ttttgaaaat tttgagtatt ttcaataaat ttgtagagga ctcagatatt gaaaaaaagc
     7501 tacagcaatt aatacttgat aagaagagta ttgagaaggg caacggttca tcatctcatg
     7561 gatctgcaca tgaacaaaca ccagagtcaa acgacgttga aattgaggct actgcgccaa
     7621 ttgatgacaa tacagacgat gataacaaac cgaagttatc tgatgtagaa aaggattaaa
     7681 gatgctaaga gatagtgatg atatttcata aataatgtaa ttctatatat gttaattacc
     7741 ttttttgcga ggcatattta tggtgaagga taagttttga ccatcaaaga aggttaatgt
     7801 ggctgtggtt tcagggtcca taaagcccac atggataaca ttacccctga attcgcatct
     7861 agactga
//
