LOCUS       sequence-475873-       11780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GAL-inducible peroxisomal sfCherry3C large fragment and 
            Pex22 peroxisomal chauffeur with high affinity SH3 peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11780)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1139
FEATURES             Location/Qualifiers
     source          1..11780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             732..1172
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1218..2153
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1266..2156
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        3458..3899
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3458..3575
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3870..3893
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5674..6384
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        6965..7372
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7373..8467
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(9498..10313)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9498..10313)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10221..10240
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10305..10324
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10538..11126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11027..11046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgagtcg aacaagaagc aggcaaagtt tagagcactg
       61 cccctccgca ctcaaaaaag aaaaaactag gaggaaaata aaattctcaa ccacacaaac
      121 acataaacac atacaaatac aaatacaagc ttatttactt gacatcgcgc gatcttccac
      181 tattcagcgc cgtccgccct ctctcgtgtt ttttgtttac gcgacaacta tgcgaaatcc
      241 ggagcaacgg gcaaccgttt ggggaaagac cacacccacg cgcgatcgcc atggcaacga
      301 ggtcgcacac gccccacacc cagacctccc tgcgagcggg catgggtaca atgtccccgt
      361 tgccacagac accacttcgt agcacagcgc agagcgtagc gtgttgttgc tgctgacaaa
      421 agaaaatttt tcttagcaaa gcaaaggagg ggaagcacgg gcagatagca ccgtaccata
      481 cccttggaaa ctcgaaatga acgaagcagg aaatgagaga atgagagttt tgtaggtata
      541 tatagcggta gtgtttgcgc gttaccatca tcttctggat ctatctattg ttcttttcct
      601 catcactttc ccctttttcg ctcttcttct tgtcttttat ttctttcttt tttttaattg
      661 ttccctcgat tggctatcta ccaaagaatc caaacttaat acacgtattt atttgtccaa
      721 ttaccagatc tatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
      781 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
      841 agtgtcgcta ctctcccaaa accaaaagat ctccgctgac tagggcacat ctgacagaag
      901 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
      961 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     1021 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     1081 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     1141 gtaacaaagg tcaaagacag ttgactgtat cgattgactc ggcaggatcc ggtgacggtg
     1201 ctggtttaat taactctgct ggagacatga gagctgccaa cctttggcca agcccgctca
     1261 tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca
     1321 gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct
     1381 tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca
     1441 tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc
     1501 accttctaga atgtgcctgg ctagagatcc tgatgattgg actcgtctgg cgctccatgg
     1561 agcacccagg gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat
     1621 gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca
     1681 tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg
     1741 gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc
     1801 gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc
     1861 tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca
     1921 tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct
     1981 atgacctgct gctggagatg ctggacgccc accgcctaca tgcgcccact agccgtggag
     2041 gggcatccgt ggaggaaacg gaccaaagcc acttggccac tgcgggctct acttcatcgc
     2101 attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc acagtcgcgg
     2161 ctgcaggtga cggtgctggt ttaattaaca tgacggtcga ccatgatttc aatagcgaag
     2221 atattttatt ccccatagaa agcatgagta gtatacaata cgtggagaat aataacccaa
     2281 ataatattaa caacgatgtt atcccgtatt ctctagatat caaaaacact gtcttagata
     2341 gtgcggatct caatgacatt caaaatcaag aaacttcact gaatttgggg cttcctccac
     2401 tatctttcga ctctccactg cccgtaacgg aaacgatacc atccactacc gataacagct
     2461 tgcatttgaa agctgatagc aacaaaaatc gcgatgcaag aactattgaa aatgatagtg
     2521 aaattaagag tactaataat gctagtggct ctggggcaaa tcaatacaca actcttactt
     2581 caccttatcc tatgaacgac attttgtaca acatgaacaa tccgttacaa tcaccgtcac
     2641 cttcatcggt acctcaaaat ccgactataa atcctcccat aaatacagca agtaacgaaa
     2701 ctaatttatc gcctcaaact tcaaatggta atgaaactct tatatctcct cgagcccaac
     2761 aacatacgtc cattaaagat aatcgtctgt ccttacctaa tggtgctaat tcgaatcttt
     2821 tcattgacac taacccaaac aatttgaacg aaaaactaag aaatcaattg aactcagata
     2881 caaattcata ttctaactcc atttctaatt caaactccaa ttctacgggt aatttaaatt
     2941 ccagttattt taattcactg aacatagact ccatgctaga tgattacgtt tctagtgatc
     3001 tcttattgaa tgatgatgat gatgacacta atttatcacg ccgaagattt agcgacgtta
     3061 taacaaacca atttccgtca atgacaaatt cgaggaatga gctcggatcc taactcgaga
     3121 ttgaattgaa ttgaaatcga tagatcaatt tttttctttt ctctttcccc atcctttacg
     3181 ctaaaataat agtttatttt attttttgaa tattttttat ttatatacgt atatatagac
     3241 tattatttat cttttaatga ttattaagat ttttattaaa aaaaaattcg ctcctctttt
     3301 aatgccttta tgcagttttt ttttcccatt cgatatttct atgtgctgcc aaaccagatg
     3361 tcaacacagc tacaacgccc cattatctta gcctaaaaaa accttctctt tggaactttc
     3421 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg
     3481 cgacagccct ccgacggatg actctcctcc gtgcgtcctc gtcatcaccg gtcgcgttcc
     3541 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     3601 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta
     3661 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3721 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc
     3781 tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa
     3841 tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3901 aaaaactata agatctatgg aggaagacaa catggctatc atcaaggaat tcatgagatt
     3961 caaggtccac atggaaggtt ctgtcaacgg tcacgaattt gaaattgaag gtgaaggtga
     4021 aggtcatcca tacgaaggta ctcaaacagc tagactaaag gttaccaagg gtgacccatt
     4081 gccttttgct tgggatatct tgtctccaca attcatgtac ggttccaagg cttacgttaa
     4141 gcacccagct gacattccag actacttgaa attatctttc ccagaaggtt tcacctggga
     4201 aagagttatg aacttcgaag acggcggagt tgttgctgtc acccaagact cctccttgca
     4261 agatggtcaa ttcatctaca aggtcaagtt gttgggtatt aatttcccat cagatggtcc
     4321 agtcatgcaa aagaagacca tgggttggga agcttccact gaaagaatgt acccagaaga
     4381 tggtgctttg aagggtgaaa tcaaccaaag attgaaatta aaggacggtg gtcactacga
     4441 tgctgaagtc aagaccactt acagagccaa gaagccagtt caattgccag gtgcctacga
     4501 tgttgacatt aaattggaca tcacttctca caacgaagat ggatccggtt cattgggaag
     4561 aggtagaaga tccaaattgt aataactcga gtggcagctt ttgattaagc cttctagtcc
     4621 aaaaaacacg tttttttgtc atttatttca ttttcttaga atagtttagt ttattcattt
     4681 tatagtcacg aatgttttat gattctatat agggttgcaa acaagcattt ttcattttat
     4741 gttaaaacaa tttcaggttt accttttatt ctgcttgtgg tgacgcgtgt atccgcccgc
     4801 tcttttggtc acccatgtat gctggatgca cacactggct taagatgaca acgtcttggg
     4861 gccttaccac cagtggactt tcttgctgtt tgctttgttc tggccattgt ttgcgtttat
     4921 atatttatgt tagatgtttt tcttattaac tagaaagaaa gaatataaaa ggttgaggaa
     4981 agagatgtat cccgaagaat acacagtctt ttatatatgt atttcaacaa ggagccgtgg
     5041 agggtactaa aaagaaaaat cgcccgggca tttcgttatc ttccacgcta aaagtcaagg
     5101 agagatatta cggccaggat cgcaaaggtg cagagcaagg aaatgtgaga aattgtgaga
     5161 acgataatgt atgggacaat gcgaaaatgt gagaacgaga gcaaaaatct tttttgtatc
     5221 tccccgccga atttggaaac cgcgttctga aaacttcgca tcttcacata gtaaaactgt
     5281 tccgagcgct tctccccata atggttagtg gtaaaaaccg aagttgttta ctttagcaaa
     5341 tgcccgcgaa tacggtggta aattgccacc cccccttccc cattcattgg gtaaagacca
     5401 atttgatgga taaattggtt gtggaaaagg tctaattctt tttcctataa ataccgagat
     5461 attttttcta tatgatggtt tccgtcgcat tattgtactc tatagtacta aagcaacaaa
     5521 caaaaacaag caacaaatat aatatagtaa aatagatcta tgccaccacc atcaagaagt
     5581 agaataaaca aaacaagaac attaggaata gtgggtacag ctatagcagt gttggtcacg
     5641 tcctactata tatatcaaaa ggtgacaggt tcttctaaag gtgaagaatt attcactggt
     5701 gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc
     5761 ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaattgat ttgtactact
     5821 ggtaaattgc cagttccatg gccaacctta gtcactactt taggttatgg tttgcaatgt
     5881 tttgctagat acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa
     5941 ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct
     6001 gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt
     6061 aaagaaggtg gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt
     6121 tacatcactg ctgacaaaca aaagaatggt atcaaagcta acttcaaaat tagacacaac
     6181 attgaagatg gtggtgttca attagctgac cattatcaac aaaatactcc aattggtgat
     6241 ggtccagtct tgttaccaga caaccattac ttatcctatc aatctgcctt atccaaagat
     6301 ccaaacgaaa agagagatca catggtcttg ttagaatttg ttactgctgc tggtattacc
     6361 catggtatgg atgaattgta caaaggatcc gggtcaggct ccggatctgg ccctccccca
     6421 gctcttccgc caaaaagacg aaggtaatgg cagtgctttt aactaagaat tattagtctt
     6481 ttctgcttat tttttcatca tagtttagaa cactttatat taacgaatag tttatgaatc
     6541 tatttaggtt taaaaattga tacagtttta taagttactt tttcaaagac tcgtgctgtc
     6601 tattgcataa tgcactggaa ggggaaaaaa aaggtgcaca cgcgtggctt tttcttgaat
     6661 ttgcagtttg aaaaatgctg agcagttaca gagatgttac gaaccactag tgcactgcag
     6721 tacatcgagg agaacttcta gtatatctac atacctaata ttattgcctt attaaaaatg
     6781 gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc ctgtacttcc
     6841 ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta tttctcaaca
     6901 agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata tcttgaccgc
     6961 agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct cttagcaacc
     7021 attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca gaatcaaatt
     7081 cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac ctttttcaac
     7141 tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa ccggcttttc atatagaata
     7201 gagaagcgtt catgactaaa tgcttgcatc acaatacttg aagttgacaa tattatttaa
     7261 ggacctattg ttttttccaa taggtggtta gcaatcgtct tactttctaa cttttcttac
     7321 cttttacatt tcagcaatat atatatatat atttcaagga tataccattc taatgtctgc
     7381 ccctaagaag atcgtcgttt tgccaggtga ccacgttggt caagaaatca cagccgaagc
     7441 cattaaggtt cttaaagcta tttctgatgt tcgttccaat gtcaagttcg atttcgaaaa
     7501 tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt ccacttccag atgaggcgct
     7561 ggaagcctcc aagaaggctg atgccgtttt gttaggtgct gtgggtggtc ctaaatgggg
     7621 aaccggtagt gttagacctg aacaaggttt actaaaaatc cgtaaagaac ttcaattgta
     7681 cgccaactta agaccatgta actttgcatc cgactctctt ttagacttat ctccaatcaa
     7741 gccacaattt gctaaaggta ctgacttcgt tgttgtcaga gaattagtgg gaggtattta
     7801 ctttggtaag agaaaggaag acgatggtga tggtgtcgct tgggatagtg aacaatacac
     7861 cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc atggccctac aacatgagcc
     7921 accattgcct atttggtcct tggataaagc taatgttttg gcctcttcaa gattatggag
     7981 aaaaactgtg gaggaaacca tcaagaacga atttcctaca ttgaaggttc aacatcaatt
     8041 gattgattct gccgccatga tcctagttaa gaacccaacc cacctaaatg gtattataat
     8101 caccagcaac atgtttggtg atatcatctc cgatgaagcc tccgttatcc caggttcctt
     8161 gggtttgttg ccatctgcgt ccttggcctc tttgccagac aagaacaccg catttggttt
     8221 gtacgaacca tgccacggtt ctgctccaga tttgccaaag aataaggtca accctatcgc
     8281 cactatcttg tctgctgcaa tgatgttgaa attgtcattg aacttgcctg aagaaggtaa
     8341 ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt atcagaactg gtgatttagg
     8401 tggttccaac agtaccaccg aagtcggtga tgctgtcgcc gaagaagtta agaaaatcct
     8461 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
     8521 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
     8581 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
     8641 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
     8701 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
     8761 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
     8821 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
     8881 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
     8941 tcgagagtac tcgtatcgca tgtcggtgcg acacgaaatt acaaaatgga atatgttcat
     9001 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     9061 atgtaaagga atacaggtaa gcaaattgat actaatggct caacgtgata aggaaaaaga
     9121 attgcacttt aacattaata ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa
     9181 cagaaaatca tgaaactatg attcctaatt tatatattgg aggattttct ctaaaaaaaa
     9241 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     9301 accttcttga accatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     9361 aacggcctta acgacgtagt cgacctcctc ttcagtacta aatctaccaa taccaaatct
     9421 gatggaagaa tgggctaatg catcatcctt acccagcgcc gagcggccgc gtgttacaac
     9481 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     9541 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     9601 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     9661 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     9721 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     9781 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     9841 ttattcattc gtgattgcgc ctgagcgagg cgaaatacgc gatcgctgtt aaaaggacaa
     9901 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     9961 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
    10021 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
    10081 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
    10141 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
    10201 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
    10261 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc
    10321 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
    10381 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
    10441 tttgctgagt tgaaggatca gtcatgacca aaatccctta acgtgagttt tcgttccact
    10501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10681 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11221 accgtgcggc cgccaatacc cagaagaaca gtaaaataaa gcaaggtacg tgaaattaat
    11281 atttttaaat ggttctaacc gatgccgaag aactgcgcag tccggttata acgtctgaca
    11341 tgtccttttt tgatttggaa tccaaccact caagtgactc tgttcattta ctttgcgaaa
    11401 aatataccca caaattgccc atcgaaagtg aatcgcaaac caccttcaga ctggcaccga
    11461 caaagcaaag attatacaga cagagtactt tatacgtacc gttaagtctc aagcaaaggg
    11521 ttttcttatt tactgaacgg gtaaagagta tctgggccgg cttgccaaga tgcaaaccga
    11581 ataagtattt caaagttgca tttgccttag ccgtcctgac accattggct atttggatat
    11641 tttatattga ctttcgtgta cattgatcac atcgactgtt ctattggcaa atgaaccacg
    11701 ggcattgact atttttcagg ttactactat atattatcat cacgggcaag gattgtaccc
    11761 tgaattcgca tctagactga
//
