LOCUS       sequence-475877-       11012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Yea4p membrane protein cargo fused directly to Pex22 TMD, 
            peroxisomal Ynd1p truncation, and peroxisomal mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11012)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1574
FEATURES             Location/Qualifiers
     source          1..11012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5105..5292
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5160..5184)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5636..6049
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6069..6779
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             7285..8088
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8730..9545)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8730..9545)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9453..9472
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9537..9556
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9770..10358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10259..10278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgccacca ccatcaagaa gtagaataaa caaaacaaga acattaggaa
      781 tagtgggtac agctatagca gtgttggtca cgtcctacta tatatatcaa aaggtgacag
      841 gttcttggaa ctcactaaaa gcattcgctc tagtgttcgg aggttgttgc tccaatgtta
      901 ttacatttga aaccttaatg agcaacgaaa caggcagcat taataatctc attacttttt
      961 gtcaattttt atttgttaca tgtcagggac tccccgaatt tctggatgtt catcaacctt
     1021 tcccttactt caagcctctg aaaactccac ttcacgttta tgtcattact gtcgttttat
     1081 tttatatttc atctacgacg aataataacg tatttaaata caacatatcc attccgattc
     1141 atatcgtttt cagatgcttc ggaaccgtaa taaccatgtt tacatgctgg ttattaaatg
     1201 gtaggaaata tacaaagatt cagattttat caacactatt tttaaccatt ggagctataa
     1261 ttgcctcatt attcaaagat gctgattttc ggtaccaaga tttgaaactg caagcgtgga
     1321 aaattggaag tgaccaatct gtagatctca cttttatttt tggcatttgt atactagtac
     1381 tttcatcatt cacatcatct ttactttcag catacaatga acgtacgtat caaaaatatg
     1441 gaaagcattg gaaggagaat atcttttaca gtcatttttt atcattacct ctctttctat
     1501 ttagtcgaaa gcagttaatt cacgaatatc gagtgatgag aaagtctgaa agaatattgt
     1561 gttcaaattt tggagggaag attctagtcc cgcgagaaga aactctcctt cttttcaatg
     1621 tgttaacaca atatttttgc gtcaagggtg ttaatatcct cgccagtaaa acaaacgctc
     1681 tgacactttc cataacatta cttgtaagaa aatttataag tcttttactg agcgtccgac
     1741 ttttcgataa taacttgtca tatactggct acatcggggt ctacctagtc ttttttggtg
     1801 cctttatata ctcgctggga tcaattcacc ccaggcaaaa tgacaagggg gcgataaaga
     1861 aaagtaaagg atccgtttct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     1921 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     1981 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     2041 catggccaac cttagtcact actttatctt ggggtgttca atgttttgca agatacccag
     2101 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     2161 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     2221 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     2281 ttttaggtca caaattggaa tacaattatt tctctgacaa tgtttacatc actgctgaca
     2341 aacaaaagaa tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg
     2401 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     2461 cagacaacca ttacttatcc actcaatcta agttatccaa agatccaaac gaaaagaggg
     2521 accacatggt cttgttagaa tttgttactg ctgctggtat taccttgggt atggatgaat
     2581 tgtacaaata actcgagtgg cgccaattgg tgcggcaatt gataataacg aaaatgtctt
     2641 ttaatgatct gggtataatg aggaattttc cgaacgtttt tactttatat atatatatac
     2701 atgtaacata tattctatac gctatagaga aaggaaattt ttcaattaaa aaaaaaatag
     2761 agaaagagtt tcacttcttg attatcgcta acactaatgg ttgaagtact gctactttaa
     2821 ttttatgctg ccaaaccaga tgtcaacaca gctacaacgt atatattaaa tttgctcttg
     2881 ttctgtactt tcctaattct tatgtaaaaa gacaagaatt tatgatacta tttaataaca
     2941 aaaaactacc taagaaaagc atcatgcagt cgaaattgaa atcgaaaagt aaaactttaa
     3001 cggaacatgt ttgaaattct aagaaagcat acatcttcat cccttatata tagagttatg
     3061 tttgatatta gtagtcatgt tgtaatctct ggcctaagta tacgtaacga aaatggtagc
     3121 acgtcgcgtt tatggccccc aggttaatgt gttctctgaa attcgcatca ctttgagaaa
     3181 taatgggaac accttacgcg tgagctgtgc ccaccgcttc gcctaataaa gcggtgttct
     3241 caaaatttct ccccgttttc aggatcacga gcgccatcta gttctggtaa aatcgcgctt
     3301 acaagaacaa agaaaagaaa catcgcgtaa tgcaacagtg agacacttgc cgtcatatat
     3361 aaggttttgg atcagtaacc gttatttgag cataacacag gtttttaaat atattattat
     3421 atatcatggt atatgtgtaa aatttttttg ctgactggtt ttgtttattt atttagcttt
     3481 ttaaaaattt tactttcttc ttgttaattt tttctgattg ctctatactc aaaccaacaa
     3541 caacttactc tacaactaag atctatgctc atagaaaaca ctaatgatcg gtttggtatc
     3601 gtcatagatg cagggtcttc gggttcgaga atccatgtgt ttaagtggca ggacacggaa
     3661 tcgcttcttc atgcaacaaa ccaggactca cagtccatat tacaatcagt acctcatatt
     3721 catcaagaaa aagactggac ttttaagctg aatccaggct tgtcgagttt tgaaaaaaaa
     3781 cctcaagatg cgtacaaatc tcacatcaag ccgttactag attttgctaa gaatattatc
     3841 cctgaatcac attggtcgag ttgtcccgtt tttattcaag caaccgcggg catgcgcctt
     3901 ttacctcaag acatacaatc ttccattttg gatggtttgt gtcaggggct caaacaccct
     3961 gcagaatttt tggttgagga ttgctcagca caaattcaag tcattgatgg tgaaaccgaa
     4021 ggtttatatg gctggcttgg cttaaactat ctatatggac actttaatga ctataatcca
     4081 gaagtctctg accattttac atttgggttt atggacatgg gcggtgcctc tactcagatt
     4141 gcgtttgcac cgcatgattc aggagaaata gctagacata gagatgacat cgccaccatc
     4201 ttcttgagga gtgttaacgg agatttgcag aaatgggacg tttttgttag tacgtggtta
     4261 gggtttggtg ccaatcaggc tagaagaagg tacttggctc agttgatcaa tacccttcca
     4321 gaaaacacaa atgattacga aaatgatgac ttctctacaa ggaacttgaa tgatccatgt
     4381 atgccgcgag gaagcagcac ggattttgaa tttaaagata ccatatttca catcgcgggt
     4441 tctgggaatt acgaacaatg tacaaaatct atttatccgt tacttttaaa gaacatgcct
     4501 tgtgatgacg agccgtgctt gtttaatggt gttcatgctc ctcgaataga ctttgccaat
     4561 gacaaattta taggtacttc tgagtactgg tacactgcca acgacgtatt caaacttggg
     4621 ggcgaataca actttgacaa atttagcaaa agcctaaggg agttttgcaa ttccaattgg
     4681 acgcaaatat tagcgaacag tgataaaggc gtgtataatt ctattccgga gaattttttg
     4741 aaagatgcat gctttaaggg caattgggtc cttaatatac ttcatgaagg gtttgatatg
     4801 cctcgaatag atgtcgacgc agaaaatgtc aatgacaggc ccttatttca aagtgtggaa
     4861 aaagtcgaag aacgagagtt atcgtggaca ctaggcagaa ttttacttta tgcctcaggt
     4921 agcatattgg caggtaatga tgacttcatg gtgggtattg cgcccagtga aagaagaact
     4981 aagctcactg gtaagaaatt catacctggc aagttactgg agtctgatca actacgcaag
     5041 caaagttccg gatccggttc attgggaaga ggtagaagat ccaaattgta ataactcgag
     5101 tggcgcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     5161 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa
     5221 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     5281 cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttcg ctggatgcac
     5341 acactggctt aagatgacaa cgccttgcca acagggagtt cttcagagac atggaggctc
     5401 aaaacgaaat tattgacagc ctagacatca atagtcatac aacagaaagc gaccacccaa
     5461 ctttggctga taatagcgta taaacaatgc atactttgta cgttcaaaat acaatgcagt
     5521 agatatattt atgcatatta catataatac atatcacata ggaagcaaca ggcgcgttgg
     5581 acttttaatt ttcgaggacc gcgaatcctt acatcacacc caatccccca caagtgatcc
     5641 cccacacacc atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg
     5701 actccgcgca tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc
     5761 tttcttcctc tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaca
     5821 ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt
     5881 tcttgaaaat tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag
     5941 ttaataaacg gtcatcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact
     6001 ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt tttaattaca
     6061 aaagatctat ggtgtccaaa ggagaggagt taatcaagga aaacatgaga atgaaagttg
     6121 tcatggaggg ctccgttaat ggtcaccaat tcaagtgtac aggggaaggt gaaggtaatc
     6181 cttacatggg tacacaaact atgagaatta aagtaattga aggcggacca ctaccatttg
     6241 catttgacat tctggcaacg tcattcatgt acggatcacg aactttcatc aagtacccta
     6301 aaggtatacc agactttttc aagcaatctt ttccagaggg ttttacatgg gaaagggtta
     6361 caagatacga agatgggggt gtcgtcacag ttatgcaaga tacttcatta gaagatggct
     6421 gccttgtcta tcatgtgcaa gtaagagggg tgaattttcc ttctaacgga cctgtgatgc
     6481 agaaaaagac caaaggttgg gaaccaaata ctgaaatgat gtacccagct gatggaggtt
     6541 tgagaggcta cacacacatg gcgcttaaag ttgatggtgg aggtcatttg tcttgtagtt
     6601 ttgttaccac ttatcgttct aaaaagactg ttggcaatat caaaatgcca ggaatacatg
     6661 ctgtagacca cagactagaa agactcgaag agagcgataa cgaaatgttc gttgtacaga
     6721 gagagcatgc cgtagccaaa tttgctggct taggcggtgg tatggatgaa ttgtataagg
     6781 gatccggttc attgggaaga ggtagaagat ccaaattgta ataactcgag tggcgccaat
     6841 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     6901 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     6961 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     7021 ctaacactaa tggttgaagt actgctactt taattttatg ctgagcagtt acagagatgt
     7081 tacgaaccac tagtgcactg cagtacacgg tttccttgaa atttttttga ttcggtaatc
     7141 tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata
     7201 tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa
     7261 acgtgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact
     7321 catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg
     7381 tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt
     7441 cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc
     7501 acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga
     7561 aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata
     7621 gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt
     7681 ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa
     7741 ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt tgacattgcg
     7801 aagagcgaca aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat
     7861 gaaggttacg attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagatgca
     7921 ttgggtcaac agtatagaac cgtggatgat gtggtttcta caggatctga cattattatt
     7981 gttggaagag gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga
     8041 aaagcaggct gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta
     8101 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
     8161 ttacccgagt agagcacttg aatccactgc cccgggaatc tcggtcgtaa tgatttctat
     8221 aatgacgaaa aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa aaaggaacta
     8281 tccaatacct cgccagaacc aagtaacagt attttacggg gcacaaatca agaacaataa
     8341 gacaggactg taaagatgga cgcattgaac tccaaagaac aacaagagtt ccaaaaagta
     8401 gtggaacaaa agcaaatgaa ggatttcatg cgtttgtact ctaatctggt agaaagatgt
     8461 ttcacagact gtgtcaatga cttcacaaca tcaaagctaa ccaataagga acaaacatgc
     8521 atcatgaagt gctcagaaaa gttcttgaag catagcgaac gtgtagggca gcgtttccaa
     8581 gaacaaaacg ctgccttggg acaaggcttg ggccgataag gtgtactggc gtatatatat
     8641 ctaattatgt atctctggtg tagcccattt ttagcatgta aatataaaga ccgagcggcc
     8701 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     8761 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     8821 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     8881 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     8941 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     9001 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     9061 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
     9121 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     9181 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     9241 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     9301 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     9361 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     9421 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     9481 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     9541 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     9601 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
     9661 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
     9721 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9781 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9841 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9901 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9961 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10021 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10081 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10141 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10201 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10261 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10321 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10381 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10441 attctgtgga taaccgtgcg gccgccaatg ctaaattcga gtgaaacaca ggaagatcag
    10501 aaaatcctca tttcatccat attaacaata atttcaaatg tttatttgca ttatttgaaa
    10561 ctaggcaaga caagcaacga aacgtttttg aaaattttga gtattttcaa taaatttgta
    10621 gaggactcag atattgaaaa aaagctacag caattaatac ttgataagaa gagtattgag
    10681 aagggcaacg gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac
    10741 gttgaaattg aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag
    10801 ttatctgatg tagaaaagga ttaaagatgc taagagatag tgatgatatt tcataaataa
    10861 tgtaattcta tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt
    10921 tttgaccatc aaagaaggtt aatgtggctg tggtttcagg gtccataaag cccacatgga
    10981 taacattacc cctgaattcg catctagact ga
//
