LOCUS       sequence-475879-       11117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Yea4p membrane protein cargo fused directly to Pex15 TMD, 
            peroxisomal Ynd1p truncation, and peroxisomal mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11117)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pVL1337
FEATURES             Location/Qualifiers
     source          1..11117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5210..5397
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5265..5289)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5741..6154
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6174..6884
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             7390..8193
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(8835..9650)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8835..9650)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9558..9577
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9642..9661
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9875..10463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10364..10383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgtggaac tcactaaaag cattcgctct agtgttcgga ggttgttgct
      781 ccaatgttat tacatttgaa accttaatga gcaacgaaac aggcagcatt aataatctca
      841 ttactttttg tcaattttta tttgttacat gtcagggact ccccgaattt ctggatgttc
      901 atcaaccttt cccttacttc aagcctctga aaactccact tcacgtttat gtcattactg
      961 tcgttttatt ttatatttca tctacgacga ataataacgt atttaaatac aacatatcca
     1021 ttccgattca tatcgttttc agatgcttcg gaaccgtaat aaccatgttt acatgctggt
     1081 tattaaatgg taggaaatat acaaagattc agattttatc aacactattt ttaaccattg
     1141 gagctataat tgcctcatta ttcaaagatg ctgattttcg gtaccaagat ttgaaactgc
     1201 aagcgtggaa aattggaagt gaccaatctg tagatctcac ttttattttt ggcatttgta
     1261 tactagtact ttcatcattc acatcatctt tactttcagc atacaatgaa cgtacgtatc
     1321 aaaaatatgg aaagcattgg aaggagaata tcttttacag tcatttttta tcattacctc
     1381 tctttctatt tagtcgaaag cagttaattc acgaatatcg agtgatgaga aagtctgaaa
     1441 gaatattgtg ttcaaatttt ggagggaaga ttctagtccc gcgagaagaa actctccttc
     1501 ttttcaatgt gttaacacaa tatttttgcg tcaagggtgt taatatcctc gccagtaaaa
     1561 caaacgctct gacactttcc ataacattac ttgtaagaaa atttataagt cttttactga
     1621 gcgtccgact tttcgataat aacttgtcat atactggcta catcggggtc tacctagtct
     1681 tttttggtgc ctttatatac tcgctgggat caattcaccc caggcaaaat gacaaggggg
     1741 cgataaagaa aagtaaaggt tctgtttcta aaggtgaaga attattcact ggtgttgtcc
     1801 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
     1861 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
     1921 tgccagttcc atggccaacc ttagtcacta ctttatcttg gggtgttcaa tgttttgcaa
     1981 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
     2041 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
     2101 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
     2161 atggtaacat tttaggtcac aaattggaat acaattattt ctctgacaat gtttacatca
     2221 ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa aattagacac aacattgaag
     2281 atggtggtgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
     2341 tcttgttacc agacaaccat tacttatcca ctcaatctaa gttatccaaa gatccaaacg
     2401 aaaagaggga ccacatggtc ttgttagaat ttgttactgc tgctggtatt accttgggta
     2461 tggatgaatt gtacaaagga tccgttctgg ctggttcatt ttgggctgtc ctgaagcatc
     2521 atttcacaag aagtgtgctg aacaaaaacg gacttctgct cacaggtctg ctgctactct
     2581 tatgtttgaa aaaatataag tcattgatgg caattttcaa acatgttcca gcggcctttc
     2641 acactgtata cccccagatt gtagggttgc taaaacttct agcgagtata taataactcg
     2701 agtggcgcca attggtgcgg caattgataa taacgaaaat gtcttttaat gatctgggta
     2761 taatgaggaa ttttccgaac gtttttactt tatatatata tatacatgta acatatattc
     2821 tatacgctat agagaaagga aatttttcaa ttaaaaaaaa aatagagaaa gagtttcact
     2881 tcttgattat cgctaacact aatggttgaa gtactgctac tttaatttta tgctgccaaa
     2941 ccagatgtca acacagctac aacgtatata ttaaatttgc tcttgttctg tactttccta
     3001 attcttatgt aaaaagacaa gaatttatga tactatttaa taacaaaaaa ctacctaaga
     3061 aaagcatcat gcagtcgaaa ttgaaatcga aaagtaaaac tttaacggaa catgtttgaa
     3121 attctaagaa agcatacatc ttcatccctt atatatagag ttatgtttga tattagtagt
     3181 catgttgtaa tctctggcct aagtatacgt aacgaaaatg gtagcacgtc gcgtttatgg
     3241 cccccaggtt aatgtgttct ctgaaattcg catcactttg agaaataatg ggaacacctt
     3301 acgcgtgagc tgtgcccacc gcttcgccta ataaagcggt gttctcaaaa tttctccccg
     3361 ttttcaggat cacgagcgcc atctagttct ggtaaaatcg cgcttacaag aacaaagaaa
     3421 agaaacatcg cgtaatgcaa cagtgagaca cttgccgtca tatataaggt tttggatcag
     3481 taaccgttat ttgagcataa cacaggtttt taaatatatt attatatatc atggtatatg
     3541 tgtaaaattt ttttgctgac tggttttgtt tatttattta gctttttaaa aattttactt
     3601 tcttcttgtt aattttttct gattgctcta tactcaaacc aacaacaact tactctacaa
     3661 ctaagatcta tgctcataga aaacactaat gatcggtttg gtatcgtcat agatgcaggg
     3721 tcttcgggtt cgagaatcca tgtgtttaag tggcaggaca cggaatcgct tcttcatgca
     3781 acaaaccagg actcacagtc catattacaa tcagtacctc atattcatca agaaaaagac
     3841 tggactttta agctgaatcc aggcttgtcg agttttgaaa aaaaacctca agatgcgtac
     3901 aaatctcaca tcaagccgtt actagatttt gctaagaata ttatccctga atcacattgg
     3961 tcgagttgtc ccgtttttat tcaagcaacc gcgggcatgc gccttttacc tcaagacata
     4021 caatcttcca ttttggatgg tttgtgtcag gggctcaaac accctgcaga atttttggtt
     4081 gaggattgct cagcacaaat tcaagtcatt gatggtgaaa ccgaaggttt atatggctgg
     4141 cttggcttaa actatctata tggacacttt aatgactata atccagaagt ctctgaccat
     4201 tttacatttg ggtttatgga catgggcggt gcctctactc agattgcgtt tgcaccgcat
     4261 gattcaggag aaatagctag acatagagat gacatcgcca ccatcttctt gaggagtgtt
     4321 aacggagatt tgcagaaatg ggacgttttt gttagtacgt ggttagggtt tggtgccaat
     4381 caggctagaa gaaggtactt ggctcagttg atcaataccc ttccagaaaa cacaaatgat
     4441 tacgaaaatg atgacttctc tacaaggaac ttgaatgatc catgtatgcc gcgaggaagc
     4501 agcacggatt ttgaatttaa agataccata tttcacatcg cgggttctgg gaattacgaa
     4561 caatgtacaa aatctattta tccgttactt ttaaagaaca tgccttgtga tgacgagccg
     4621 tgcttgttta atggtgttca tgctcctcga atagactttg ccaatgacaa atttataggt
     4681 acttctgagt actggtacac tgccaacgac gtattcaaac ttgggggcga atacaacttt
     4741 gacaaattta gcaaaagcct aagggagttt tgcaattcca attggacgca aatattagcg
     4801 aacagtgata aaggcgtgta taattctatt ccggagaatt ttttgaaaga tgcatgcttt
     4861 aagggcaatt gggtccttaa tatacttcat gaagggtttg atatgcctcg aatagatgtc
     4921 gacgcagaaa atgtcaatga caggccctta tttcaaagtg tggaaaaagt cgaagaacga
     4981 gagttatcgt ggacactagg cagaatttta ctttatgcct caggtagcat attggcaggt
     5041 aatgatgact tcatggtggg tattgcgccc agtgaaagaa gaactaagct cactggtaag
     5101 aaattcatac ctggcaagtt actggagtct gatcaactac gcaagcaaag ttccggatcc
     5161 ggttcattgg gaagaggtag aagatccaaa ttgtaataac tcgagtggcg cgaatttctt
     5221 atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta
     5281 aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt
     5341 caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcat
     5401 gccgagcaaa tgcctgcaaa tcgctcccca tttcgctgga tgcacacact ggcttaagat
     5461 gacaacgcct tgccaacagg gagttcttca gagacatgga ggctcaaaac gaaattattg
     5521 acagcctaga catcaatagt catacaacag aaagcgacca cccaactttg gctgataata
     5581 gcgtataaac aatgcatact ttgtacgttc aaaatacaat gcagtagata tatttatgca
     5641 tattacatat aatacatatc acataggaag caacaggcgc gttggacttt taattttcga
     5701 ggaccgcgaa tccttacatc acacccaatc ccccacaagt gatcccccac acaccatagc
     5761 ttcaaaatgt ttctactcct tttttactct tccagatttt ctcggactcc gcgcatcgcc
     5821 gtaccacttc aaaacaccca agcacagcat actaaatttc ccctctttct tcctctaggg
     5881 tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa agacaccgcc tcgtttcttt
     5941 ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt ctttttcttg aaaatttttt
     6001 tttttgattt ttttctcttt cgatgacctc ccattgatat ttaagttaat aaacggtcat
     6061 caatttctca agtttcagtt tcatttttct tgttctatta caactttttt tacttcttgc
     6121 tcattagaaa gaaagcatag caatctaatc taagttttaa ttacaaaaga tctatggtgt
     6181 ccaaaggaga ggagttaatc aaggaaaaca tgagaatgaa agttgtcatg gagggctccg
     6241 ttaatggtca ccaattcaag tgtacagggg aaggtgaagg taatccttac atgggtacac
     6301 aaactatgag aattaaagta attgaaggcg gaccactacc atttgcattt gacattctgg
     6361 caacgtcatt catgtacgga tcacgaactt tcatcaagta ccctaaaggt ataccagact
     6421 ttttcaagca atcttttcca gagggtttta catgggaaag ggttacaaga tacgaagatg
     6481 ggggtgtcgt cacagttatg caagatactt cattagaaga tggctgcctt gtctatcatg
     6541 tgcaagtaag aggggtgaat tttccttcta acggacctgt gatgcagaaa aagaccaaag
     6601 gttgggaacc aaatactgaa atgatgtacc cagctgatgg aggtttgaga ggctacacac
     6661 acatggcgct taaagttgat ggtggaggtc atttgtcttg tagttttgtt accacttatc
     6721 gttctaaaaa gactgttggc aatatcaaaa tgccaggaat acatgctgta gaccacagac
     6781 tagaaagact cgaagagagc gataacgaaa tgttcgttgt acagagagag catgccgtag
     6841 ccaaatttgc tggcttaggc ggtggtatgg atgaattgta taagggatcc ggttcattgg
     6901 gaagaggtag aagatccaaa ttgtaataac tcgagtggcg ccaattggtg cggcaattga
     6961 taataacgaa aatgtctttt aatgatctgg gtataatgag gaattttccg aacgttttta
     7021 ctttatatat atatatacat gtaacatata ttctatacgc tatagagaaa ggaaattttt
     7081 caattaaaaa aaaaatagag aaagagtttc acttcttgat tatcgctaac actaatggtt
     7141 gaagtactgc tactttaatt ttatgctgag cagttacaga gatgttacga accactagtg
     7201 cactgcagta cacggtttcc ttgaaatttt tttgattcgg taatctccga acagaaggaa
     7261 gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa
     7321 acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacgtg caggaaacga
     7381 agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt
     7441 gctgccaagc tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat
     7501 gttcgtacca ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta
     7561 ctaaaaacac atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta
     7621 aaggcattat ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt
     7681 ggtaatacag tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac
     7741 attacgaatg cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggca
     7801 gaagaagtaa caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc
     7861 tccctatcta ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat
     7921 tttgttatcg gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg
     7981 ttgattatga cacccggtgt gggtttagat gacaagggag atgcattggg tcaacagtat
     8041 agaaccgtgg atgatgtggt ttctacagga tctgacatta ttattgttgg aagaggacta
     8101 tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa
     8161 gcatatttga gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt
     8221 atactaaact cacaaattag agcttcaatt taattatatc agttattacc cgagtagagc
     8281 acttgaatcc actgccccgg gaatctcggt cgtaatgatt tctataatga cgaaaaaaaa
     8341 aaaattggaa agaaaaagct tcatggcctt tataaaaagg aactatccaa tacctcgcca
     8401 gaaccaagta acagtatttt acggggcaca aatcaagaac aataagacag gactgtaaag
     8461 atggacgcat tgaactccaa agaacaacaa gagttccaaa aagtagtgga acaaaagcaa
     8521 atgaaggatt tcatgcgttt gtactctaat ctggtagaaa gatgtttcac agactgtgtc
     8581 aatgacttca caacatcaaa gctaaccaat aaggaacaaa catgcatcat gaagtgctca
     8641 gaaaagttct tgaagcatag cgaacgtgta gggcagcgtt tccaagaaca aaacgctgcc
     8701 ttgggacaag gcttgggccg ataaggtgta ctggcgtata tatatctaat tatgtatctc
     8761 tggtgtagcc catttttagc atgtaaatat aaagaccgag cggccgcgtg ttacaaccaa
     8821 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     8881 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     8941 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     9001 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     9061 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     9121 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     9181 ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta
     9241 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     9301 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     9361 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     9421 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     9481 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     9541 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     9601 gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     9661 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     9721 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     9781 gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9841 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9901 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9961 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10021 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10081 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10141 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10201 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10261 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10321 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10381 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10441 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10501 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10561 gtgcggccgc caatgctaaa ttcgagtgaa acacaggaag atcagaaaat cctcatttca
    10621 tccatattaa caataatttc aaatgtttat ttgcattatt tgaaactagg caagacaagc
    10681 aacgaaacgt ttttgaaaat tttgagtatt ttcaataaat ttgtagagga ctcagatatt
    10741 gaaaaaaagc tacagcaatt aatacttgat aagaagagta ttgagaaggg caacggttca
    10801 tcatctcatg gatctgcaca tgaacaaaca ccagagtcaa acgacgttga aattgaggct
    10861 actgcgccaa ttgatgacaa tacagacgat gataacaaac cgaagttatc tgatgtagaa
    10921 aaggattaaa gatgctaaga gatagtgatg atatttcata aataatgtaa ttctatatat
    10981 gttaattacc ttttttgcga ggcatattta tggtgaagga taagttttga ccatcaaaga
    11041 aggttaatgt ggctgtggtt tcagggtcca taaagcccac atggataaca ttacccctga
    11101 attcgcatct agactga
//
