LOCUS       sequence-475887-       11408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Vba2p membrane protein cargo fused directly to Pex22 TMD, 
            peroxisomal Ynd1p truncation, and peroxisomal mRuby2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11408)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1575
FEATURES             Location/Qualifiers
     source          1..11408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5501..5688
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5556..5580)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        6032..6445
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6465..7175
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             7681..8484
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9126..9941)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9126..9941)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9849..9868
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9933..9952
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10166..10754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10655..10674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgccacca ccatcaagaa gtagaataaa caaaacaaga acattaggaa
      781 tagtgggtac agctatagca gtgttggtca cgtcctacta tatatatcaa aaggtgacag
      841 gttctagtat ttcaaattgg atcaccactg cgtatttaat tacatcaaca tcttttcaac
      901 ctctttatgg gtcattttct gatgcacttg gtcgaagaaa ctgccttttc tttgctaatg
      961 gggcttttac cattggatgt ctagcctgtg gtttctcgaa aaacatctac atgcttagtt
     1021 ttatgagagc attgacaggc ataggaggtg gtggcttgat cacactttct acaatcgtaa
     1081 attcagacgt tattccaagt tcgaaaagag gaatttttca agcgtttcag aatttacttt
     1141 tgggatttgg tgccatatgt ggagcgtctt tcggtggcac aatagcgtcg agcattggtt
     1201 ggaggtggtg ttttctcatc caagtaccca tatctgtgat tagttccata ttaatgaatt
     1261 attatgtacc taatcagaaa gaatataatc gtcaaaattc tagcatattc caaaatcccg
     1321 gaaaaatact cagggacata gatgttatgg gctcaattct tattataact ggactcacac
     1381 tacagcttct ttacctgagc ctggggtgtt ctacttctaa attatcatgg accagccctt
     1441 ctgtgctact gctattagtt gggagtgtaa taatcctctt actgttcata ttgcacgaaa
     1501 ggaaaacaag tgctagagcg attattccta tggagctggt caattcctcc tacagtgtcg
     1561 ttgtactttc gataagtata cttgttggtt ttgccagcta cgcgtatctt tttactttac
     1621 cattattctt tcagattgta cttggagatt ccactgcaaa agcaggatta cgtcttacga
     1681 ttccttccct atttactccg gtaggcagtc tcataacagg attttccatg agcaagtaca
     1741 actgtctaag attattactc tacattggta tttctttgat gtttttgggt aactttttat
     1801 tcctgtttat tgaaaaaact tctccgaact ggttgattgg tctatttttg atacctgcaa
     1861 atctaggaca aggtatcact tttcctacga ccttgtttac tttcatattt atgttctcta
     1921 agagtgacca agctactgcg acatcaactt tatatttatt ccgtagtatt ggatctgtat
     1981 ggggtgttgc aatttcagct ggcgtcattc aattatcttt cgcaggttta ttgcgtagta
     2041 atttgaaagg tctactggat gaaaacaaga taaagaaact tattgttcag cttagtgcaa
     2101 actcctcata tattggatct ttacatggcg aagttaaaaa cacagtcata aagagttttg
     2161 atgaggcaac aaagagggct catctaatgt ctacattact ctcttcattg gccctgatac
     2221 tctgcatcct taaagacaat ctggcgaaac ctaaaacaag aagaggatcc gtttctaaag
     2281 gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt gatgttaatg
     2341 gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt aaattgacct
     2401 taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta gtcactactt
     2461 tatcttgggg tgttcaatgt tttgcaagat acccagatca tatgaaacaa catgactttt
     2521 tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc aaagatgacg
     2581 gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt aatagaatcg
     2641 aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa ttggaataca
     2701 attatttctc tgacaatgtt tacatcactg ctgacaaaca aaagaatggt atcaaagcta
     2761 acttcaaaat tagacacaac attgaagatg gtggtgttca attagctgac cattatcaac
     2821 aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac ttatccactc
     2881 aatctaagtt atccaaagat ccaaacgaaa agagggacca catggtcttg ttagaatttg
     2941 ttactgctgc tggtattacc ttgggtatgg atgaattgta caaataactc gagtggcgcc
     3001 aattggtgcg gcaattgata ataacgaaaa tgtcttttaa tgatctgggt ataatgagga
     3061 attttccgaa cgtttttact ttatatatat atatacatgt aacatatatt ctatacgcta
     3121 tagagaaagg aaatttttca attaaaaaaa aaatagagaa agagtttcac ttcttgatta
     3181 tcgctaacac taatggttga agtactgcta ctttaatttt atgctgccaa accagatgtc
     3241 aacacagcta caacgtatat attaaatttg ctcttgttct gtactttcct aattcttatg
     3301 taaaaagaca agaatttatg atactattta ataacaaaaa actacctaag aaaagcatca
     3361 tgcagtcgaa attgaaatcg aaaagtaaaa ctttaacgga acatgtttga aattctaaga
     3421 aagcatacat cttcatccct tatatataga gttatgtttg atattagtag tcatgttgta
     3481 atctctggcc taagtatacg taacgaaaat ggtagcacgt cgcgtttatg gcccccaggt
     3541 taatgtgttc tctgaaattc gcatcacttt gagaaataat gggaacacct tacgcgtgag
     3601 ctgtgcccac cgcttcgcct aataaagcgg tgttctcaaa atttctcccc gttttcagga
     3661 tcacgagcgc catctagttc tggtaaaatc gcgcttacaa gaacaaagaa aagaaacatc
     3721 gcgtaatgca acagtgagac acttgccgtc atatataagg ttttggatca gtaaccgtta
     3781 tttgagcata acacaggttt ttaaatatat tattatatat catggtatat gtgtaaaatt
     3841 tttttgctga ctggttttgt ttatttattt agctttttaa aaattttact ttcttcttgt
     3901 taattttttc tgattgctct atactcaaac caacaacaac ttactctaca actaagatct
     3961 atgctcatag aaaacactaa tgatcggttt ggtatcgtca tagatgcagg gtcttcgggt
     4021 tcgagaatcc atgtgtttaa gtggcaggac acggaatcgc ttcttcatgc aacaaaccag
     4081 gactcacagt ccatattaca atcagtacct catattcatc aagaaaaaga ctggactttt
     4141 aagctgaatc caggcttgtc gagttttgaa aaaaaacctc aagatgcgta caaatctcac
     4201 atcaagccgt tactagattt tgctaagaat attatccctg aatcacattg gtcgagttgt
     4261 cccgttttta ttcaagcaac cgcgggcatg cgccttttac ctcaagacat acaatcttcc
     4321 attttggatg gtttgtgtca ggggctcaaa caccctgcag aatttttggt tgaggattgc
     4381 tcagcacaaa ttcaagtcat tgatggtgaa accgaaggtt tatatggctg gcttggctta
     4441 aactatctat atggacactt taatgactat aatccagaag tctctgacca ttttacattt
     4501 gggtttatgg acatgggcgg tgcctctact cagattgcgt ttgcaccgca tgattcagga
     4561 gaaatagcta gacatagaga tgacatcgcc accatcttct tgaggagtgt taacggagat
     4621 ttgcagaaat gggacgtttt tgttagtacg tggttagggt ttggtgccaa tcaggctaga
     4681 agaaggtact tggctcagtt gatcaatacc cttccagaaa acacaaatga ttacgaaaat
     4741 gatgacttct ctacaaggaa cttgaatgat ccatgtatgc cgcgaggaag cagcacggat
     4801 tttgaattta aagataccat atttcacatc gcgggttctg ggaattacga acaatgtaca
     4861 aaatctattt atccgttact tttaaagaac atgccttgtg atgacgagcc gtgcttgttt
     4921 aatggtgttc atgctcctcg aatagacttt gccaatgaca aatttatagg tacttctgag
     4981 tactggtaca ctgccaacga cgtattcaaa cttgggggcg aatacaactt tgacaaattt
     5041 agcaaaagcc taagggagtt ttgcaattcc aattggacgc aaatattagc gaacagtgat
     5101 aaaggcgtgt ataattctat tccggagaat tttttgaaag atgcatgctt taagggcaat
     5161 tgggtcctta atatacttca tgaagggttt gatatgcctc gaatagatgt cgacgcagaa
     5221 aatgtcaatg acaggccctt atttcaaagt gtggaaaaag tcgaagaacg agagttatcg
     5281 tggacactag gcagaatttt actttatgcc tcaggtagca tattggcagg taatgatgac
     5341 ttcatggtgg gtattgcgcc cagtgaaaga agaactaagc tcactggtaa gaaattcata
     5401 cctggcaagt tactggagtc tgatcaacta cgcaagcaaa gttccggatc cggttcattg
     5461 ggaagaggta gaagatccaa attgtaataa ctcgagtggc gcgaatttct tatgatttat
     5521 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     5581 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     5641 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccgagcaa
     5701 atgcctgcaa atcgctcccc atttcgctgg atgcacacac tggcttaaga tgacaacgcc
     5761 ttgccaacag ggagttcttc agagacatgg aggctcaaaa cgaaattatt gacagcctag
     5821 acatcaatag tcatacaaca gaaagcgacc acccaacttt ggctgataat agcgtataaa
     5881 caatgcatac tttgtacgtt caaaatacaa tgcagtagat atatttatgc atattacata
     5941 taatacatat cacataggaa gcaacaggcg cgttggactt ttaattttcg aggaccgcga
     6001 atccttacat cacacccaat cccccacaag tgatccccca cacaccatag cttcaaaatg
     6061 tttctactcc ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt
     6121 caaaacaccc aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa
     6181 ttacccgtac taaaggtttg gaaaagaaaa aagacaccgc ctcgtttctt tttcttcgtc
     6241 gaaaaaggca ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt
     6301 tttttctctt tcgatgacct cccattgata tttaagttaa taaacggtca tcaatttctc
     6361 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ctcattagaa
     6421 agaaagcata gcaatctaat ctaagtttta attacaaaag atctatggtg tccaaaggag
     6481 aggagttaat caaggaaaac atgagaatga aagttgtcat ggagggctcc gttaatggtc
     6541 accaattcaa gtgtacaggg gaaggtgaag gtaatcctta catgggtaca caaactatga
     6601 gaattaaagt aattgaaggc ggaccactac catttgcatt tgacattctg gcaacgtcat
     6661 tcatgtacgg atcacgaact ttcatcaagt accctaaagg tataccagac tttttcaagc
     6721 aatcttttcc agagggtttt acatgggaaa gggttacaag atacgaagat gggggtgtcg
     6781 tcacagttat gcaagatact tcattagaag atggctgcct tgtctatcat gtgcaagtaa
     6841 gaggggtgaa ttttccttct aacggacctg tgatgcagaa aaagaccaaa ggttgggaac
     6901 caaatactga aatgatgtac ccagctgatg gaggtttgag aggctacaca cacatggcgc
     6961 ttaaagttga tggtggaggt catttgtctt gtagttttgt taccacttat cgttctaaaa
     7021 agactgttgg caatatcaaa atgccaggaa tacatgctgt agaccacaga ctagaaagac
     7081 tcgaagagag cgataacgaa atgttcgttg tacagagaga gcatgccgta gccaaatttg
     7141 ctggcttagg cggtggtatg gatgaattgt ataagggatc cggttcattg ggaagaggta
     7201 gaagatccaa attgtaataa ctcgagtggc gccaattggt gcggcaattg ataataacga
     7261 aaatgtcttt taatgatctg ggtataatga ggaattttcc gaacgttttt actttatata
     7321 tatatataca tgtaacatat attctatacg ctatagagaa aggaaatttt tcaattaaaa
     7381 aaaaaataga gaaagagttt cacttcttga ttatcgctaa cactaatggt tgaagtactg
     7441 ctactttaat tttatgctga gcagttacag agatgttacg aaccactagt gcactgcagt
     7501 acacggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     7561 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     7621 tgcccagtat tcttaaccca actgcacaga acaaaaacgt gcaggaaacg aagataaatc
     7681 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     7741 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     7801 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     7861 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     7921 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
     7981 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     8041 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
     8101 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
     8161 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     8221 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     8281 acacccggtg tgggtttaga tgacaaggga gatgcattgg gtcaacagta tagaaccgtg
     8341 gatgatgtgg tttctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     8401 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     8461 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     8521 tcacaaatta gagcttcaat ttaattatat cagttattac ccgagtagag cacttgaatc
     8581 cactgccccg ggaatctcgg tcgtaatgat ttctataatg acgaaaaaaa aaaaattgga
     8641 aagaaaaagc ttcatggcct ttataaaaag gaactatcca atacctcgcc agaaccaagt
     8701 aacagtattt tacggggcac aaatcaagaa caataagaca ggactgtaaa gatggacgca
     8761 ttgaactcca aagaacaaca agagttccaa aaagtagtgg aacaaaagca aatgaaggat
     8821 ttcatgcgtt tgtactctaa tctggtagaa agatgtttca cagactgtgt caatgacttc
     8881 acaacatcaa agctaaccaa taaggaacaa acatgcatca tgaagtgctc agaaaagttc
     8941 ttgaagcata gcgaacgtgt agggcagcgt ttccaagaac aaaacgctgc cttgggacaa
     9001 ggcttgggcc gataaggtgt actggcgtat atatatctaa ttatgtatct ctggtgtagc
     9061 ccatttttag catgtaaata taaagaccga gcggccgcgt gttacaacca attaaccaat
     9121 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     9181 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     9241 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     9301 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     9361 acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca
     9421 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     9481 gattgcgcct gagcgaggcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     9541 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     9601 ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat
     9661 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     9721 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     9781 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     9841 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     9901 cgcggcctgg agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg
     9961 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
    10021 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
    10081 aaggatcagt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtgcggccg
    10861 ccaatgctaa attcgagtga aacacaggaa gatcagaaaa tcctcatttc atccatatta
    10921 acaataattt caaatgttta tttgcattat ttgaaactag gcaagacaag caacgaaacg
    10981 tttttgaaaa ttttgagtat tttcaataaa tttgtagagg actcagatat tgaaaaaaag
    11041 ctacagcaat taatacttga taagaagagt attgagaagg gcaacggttc atcatctcat
    11101 ggatctgcac atgaacaaac accagagtca aacgacgttg aaattgaggc tactgcgcca
    11161 attgatgaca atacagacga tgataacaaa ccgaagttat ctgatgtaga aaaggattaa
    11221 agatgctaag agatagtgat gatatttcat aaataatgta attctatata tgttaattac
    11281 cttttttgcg aggcatattt atggtgaagg ataagttttg accatcaaag aaggttaatg
    11341 tggctgtggt ttcagggtcc ataaagccca catggataac attacccctg aattcgcatc
    11401 tagactga
//
