LOCUS       sequence-475892-       15236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  proUBI10::VIH2-FL::FLAG-C696A ubiquitous expression in Arabidopsis 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 15236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: proUBI10::VIH2-FL::FLAG-C696A
FEATURES             Location/Qualifiers
     source          1..15236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    186..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(252..271)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     412..434
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      670..864
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(930..2003)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2432..3061)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4362..4386
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9091..9102)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9579..9644
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      9652..9904
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13032..13056
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(13434..14225)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      14648..15236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15137..15156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
       61 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      181 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgactgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
      481 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
      541 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
      601 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
      661 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
      721 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
      781 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
      841 ttcccaatgt acgtgctatc cacaggaaag agtccttttc gacctttttc ccctgctagg
      901 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
      961 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     1021 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     1081 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     1141 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     1201 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     1261 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     1321 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     1381 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     1441 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     1501 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     1561 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     1621 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     1681 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     1741 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     1801 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     1861 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     1921 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     1981 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     2041 agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     2101 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     2161 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     2221 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     2281 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     2341 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     2401 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     2461 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     2521 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     2581 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     2641 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     2701 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     2761 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     2821 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     2881 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     2941 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
     3001 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
     3061 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
     3121 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
     3181 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
     3241 atgcagccgc acggttgaga tgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
     3301 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
     3361 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
     3421 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
     3481 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
     3541 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
     3601 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
     3661 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
     3721 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
     3781 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
     3841 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
     3901 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
     3961 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
     4021 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
     4081 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
     4141 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagaaggctg cactgaacgt
     4201 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
     4261 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
     4321 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
     4381 ctgtcaaaca ctgatagttt aaaccagaga agacaacaga gcggagtcta gctcaacaga
     4441 gcttttaacc caaattggta caatagaata caactttaga tcataattct caaaagaaag
     4501 agattcctta gctattctat ctgccactcc atttccttct cggcttgtat gcacaagcat
     4561 aaaatcctca aacttgctaa gtagatactt tatgtcttgg ataattggat tgagacttga
     4621 caagcataac tttcatgtaa ccaaagacac aagttgctga gaatccacct caaaaatgat
     4681 cttcctataa ttgaatcggg ataatgacag cacagcccat ctaagagcct ccacttctac
     4741 ttccagcacg cttcttactt ttaccacagc tcttgcacct aaccataaca ccttccctgt
     4801 atgatcgcga agcacccacc ctaagccaca ttttaatcct tctgttggcc atgccccatc
     4861 aaagttgcac ttaacccaag attgtggtgg agcttcccat gtttctcgtc tgtcccgacg
     4921 gtgttgtggt tggtgctttc cttacattct gagcctcttt ccttctaatc cactcatctg
     4981 catcttcttg tgtccttact aatacctcat tggttccaaa ttccctccct ttaagcacca
     5041 gctcgtttct gttcttccac agcctcccaa gtatccaagg gactaaagcc tccacattct
     5101 tcagatcagg atattcttgt ttaagatgtt gaactctatg gaggtttgta tgaactgatg
     5161 atctaggacc ggataagttc ccttcttcat agcgaactta ttcaaagaat gttttgtgta
     5221 tcattcttgt tacattgtta ttaatgaaaa aatattattg gtcattggac tgaacacgag
     5281 tgttaaatat ggaccaggcc ccaaataaga tccattgata tatgaattaa ataacaagaa
     5341 taaatcgagt caccaaacca cttgcctttt ttaacgagac ttgttcacca acttgataca
     5401 aaagtcatta tcctatgcaa atcaataatc atacaaaaat atccaataac actaaaaaat
     5461 taaaagaaat ggataatttc acaatatgtt atacgataaa gaagttactt ttccaagaaa
     5521 ttcactgatt ttataagccc acttgcatta gataaatggc aaaaaaaaac aaaaaggaaa
     5581 agaaataaag cacgaagaat tctagaaaat acgaaatacg cttcaatgca gtgggaccca
     5641 cggttcaatt attgccaatt ttcagctcca ccgtatattt aaaaaataaa acgataatgc
     5701 taaaaaaata taaatcgtaa cgatcgttaa atctcaacgg ctggatctta tgacgaccgt
     5761 tagaaattgt ggttgtcgac gagtcagtaa taaacggcgt caaagtggtt gcagccggca
     5821 cacacgagtc gtgtttatca actcaaagca caaatacttt tcctcaacct aaaaataagg
     5881 caattagcca aaaacaactt tgcgtgtaaa caacgctcaa tacacgtgtc attttattat
     5941 tagctattgc ttcaccgcct tagctttctc gtgacctagt cgtcctcgtc ttttcttctt
     6001 cttcttctat aaaacaatac ccaaagagct cttcttcttc acaattcaga tttcaatttc
     6061 tcaaaatctt aaaaactttc tctcaattct ctctaccgtg atcaaggtaa atttctgtgt
     6121 tccttattct ctcaaaatct tcgattttgt tttcgttcga tcccaatttc gtatatgttc
     6181 tttggtttag attctgttaa tcttagatcg aatacgattt tctgggtttg atcgttagat
     6241 atcatcttaa ttctcgatta gggtttcata gatatcatcc gatttgttca aataatttga
     6301 gttttgtcga ataattactc ttcgatttgt gatttctatc tagatctggt gttagtttct
     6361 agtttgtgcg atcgaatttg tcgattaatc tgagtttttc tgattaacag tctggaagca
     6421 ccatggagat ggaagaagga gcaagtggtg ttggtgagaa gataaagatt ggagtctgcg
     6481 tcatggaaaa gaaggttttc tcagctccca tgggggaaat tctcgacaga ctccagtctt
     6541 ttggtgaatt tgagatctta cattttgggg ataaagttat acttgaagat ccaatagaaa
     6601 gttggcccat ttgtgattgc ttgattgctt ttcattcctc tggatatcct cttgagaagg
     6661 ctcaggcata tgctgcttta agaaagccat ttttagtgaa tgaacttgat ccgcaatatc
     6721 ttcttcatga tcgccggaag gtgtatgagc atcttgagat gtatggcatc ccagttccta
     6781 ggtatgcttg tgtcaataga aaggtaccaa atcaagacct tcattatttt gtcgaggaag
     6841 aagattttgt tgaggtccat ggtgaacgct tctggaagcc atttgtggaa aagcctgtca
     6901 atggagatga ccatagtata atgatatact accctagctc ggcaggtgga ggcatgaaag
     6961 aattgtttcg caagattggg aaccgatcaa gtgaatttca tcctgacgtc agaagggtaa
     7021 ggagagaagg ctcttatata tacgaggagt ttatggccac cggaggaact gatgtcaagg
     7081 tctatacagt gggtcctgaa tacgcacatg ctgaagcaag aaagtcacct gttgttgatg
     7141 gtgttgttat gaggaataca gatgggaagg aagtgagata tccagtattg cttacacctg
     7201 ctgaaaagca aatggctaga gaagtttgca ttgcatttag gcaagcggtc tgtgggtttg
     7261 atcttctacg atctgagggc tgttcatatg tttgtgatgt caatggatgg agttttgtga
     7321 agaactcata caagtattat gacgatgctg cgtgtgtgct aaggaaaatg tgtttggatg
     7381 caaaagctcc tcatctctca tccactcttc ctcccacctt gccatggaag gtcaatgaac
     7441 ctgtacaatc taatgaaggt ctaactcgcc agggaagtgg catcatcggc acttttgggc
     7501 agtcagaaga gctacgttgt gtcattgctg ttgttcgaca tggcgataga actcccaagc
     7561 agaaagtgaa actaaaagtt acagaggaaa aactattaaa cctaatgttg aagtacaatg
     7621 gtggaaagcc aagagctgaa accaaactta agagtgccgt ccagttgcaa gatctattag
     7681 atgccacaag aatgttagtt ccccgtacaa gaccaggtcg tgaaagtgat agtgatgcag
     7741 aggaccttga acatgctgag aagcttcggc aagttaaagc agtccttgaa gagggaggac
     7801 atttctctgg tatatacagg aaggttcaac tgaagccgct gaagtgggtt aaaataccaa
     7861 aaagcgatgg tgatggcgaa gaagaaagac cagtagaggc ccttatggtt ctgaaatatg
     7921 gcggtgtttt aacacacgct ggtagaaagc aggcagaaga acttggtaga tactttcgaa
     7981 acaatatgta tccaggtgaa gggactggtt tgcttcgtct ccatagtacg taccgtcatg
     8041 accttaaaat ttacagctct gacgagggac gtgttcagat gtctgcagcg gcttttgcta
     8101 aaggcctgct tgacctagaa ggacagctga cgccaatcct ggtttctttg gttagcaagg
     8161 actcttccat gttggatggt cttgataatg ccagcattga aatggaagcg gccaaggcta
     8221 gattgaatga gattgtaacg tctggcacaa agatgataga cgacgaccaa gtctcctctg
     8281 aagatttccc ttggatgact gatggagctg gacttcctcc caacgctcat gaactcctac
     8341 gtgaactggt gaaattaact aagaatgtga ctgaacaagt aagactactt gcaatggatg
     8401 aggacgagaa cctcactgag ccatacgata taattcctcc atatgatcaa gcaaaagccc
     8461 ttggcaagac aaacattgac agtgatcgga ttgcttctgg attaccagca ggtagtgaag
     8521 gattccttct gatgtttgct cggtggataa aacttgctag ggatctctac aatgaaagaa
     8581 aagaccgatt tgacatcaca cagattccgg atgtttacga ttcatgcaag tacgacctgt
     8641 tacataattc ccatctcgat ctaaaaggat tagatgaact cttcaaagta gcacagttac
     8701 ttgcagatgg tgtaatccca aatgagtatg gcatcaatcc gcaacaaaag cttaaaatcg
     8761 gttcaaagat tgctcggcgc ttaatgggga aaatcttgat agacttgagg aatactcgag
     8821 aagaagcact gagcgttgcc gagttgaaag aaagccaaga acaagtcttg tcattatccg
     8881 cctcgcaaag ggaagataga aatagtcaac cgaagctatt tatcaatagc gatgaattga
     8941 gacgacctgg cacaggtgat aaagatgaag atgatgataa agaaaccaaa taccgattag
     9001 atccaaagta tgcaaatgtg aaaacacctg aacgtcatgt gaggactcga ctttacttca
     9061 catctgaatc gcatattcat tcgctcatga acgtccttcg atactgtaac cttgatgaat
     9121 ctctcctagg agaagaaagc ctcatttgcc aaaacgcctt agaacgtctc tgtaaaacca
     9181 aggaactcga ttacatgagt tacattgtcc taaggctctt tgagaacacc gaggtatcac
     9241 ttgaggaccc gaaaagattc cgcattgaac ttacatttag ccgtggagct gatttgtctc
     9301 ccttaaggaa taatgacgac gaggcagaga cattgctaag ggaacacaca cttccgataa
     9361 tgggaccaga gaggcttcaa gaagtaggtt cttgtttaag cttggagaca atggagaaaa
     9421 tggttcgtcc atttgctatg ccggcggaag atttccctcc ggcgtccact ccggttggtt
     9481 tctcgggtta cttctcaaaa agtgctgcgg tgctggagcg actcgtaaac ctcttccaca
     9541 actataagaa ctcttcttct aatggaagga gcaagggaga ttacaaagac catgatggag
     9601 attataagga tcacgatatc gactacaaag atgacgacga taaataaaat cgatcgttca
     9661 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     9721 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     9781 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     9841 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     9901 gatctgaggt atgtaggtat agtaacatga acaagaacag actcaagtac aagatcgcat
     9961 acgaaaatgg aaatggcaat gtcacttcca cataatcaaa cacgaatcct catatcaaca
    10021 aggcctgaga ttctaactag ctcataacaa cttagccaat agttacttga gactaccaaa
    10081 tgtatgtaga actaaagact aagggacaga gagttcgtct aaacaggtga atctagtcgt
    10141 tgttatctaa taaacaattc agccccaaat gcagaacaca catagagctc tctattgatt
    10201 caaattacga tctgatactg ataacgtcta gatttttagg gttaaagcaa tcaatcacct
    10261 gacgattcaa ggtggttgga tcatgacgat tccagaaaac atcaagcaag ctctcaaagc
    10321 tacactcttt gggatcatac tgaactctaa caacctcgtt atgtcccgta gtgccagtac
    10381 agacatcctc gtaactcgga ttgtgcacga tgccatggct atacccaacc tcggtcttgg
    10441 tcacaccagg aactctctgg taagctagct ccactcccca gaaacaaccg gcgccaaatt
    10501 gcgcgaattg ctgacctgat gacggaacat catcgtcggg tccttgggcg attgcggcgg
    10561 aagatgggtc agcttgggct tgaggacgag agccgaatcc gagtctgttg aaaaggttgt
    10621 tcattgggga tttgtatacg gagattggtc gtcgagaggt ttgagggaaa ggacaaatgg
    10681 gtttggctct ggagaaagag agtgcggctt tagagagaga attgagaggt ttagagagag
    10741 atgcggcggc gatgagcgtg ccgaggagag acgacgagga cctgcattat caaagcagtg
    10801 acgtggtgaa atttggaact tttaagaggc agatagattt attatttgta tccattttct
    10861 tcattgttct agaatgtcgc ggaacaaatt ttaaaactaa atcctaaatt tttctaattt
    10921 tgttgccaat agtggatatg tgggccgtat agaaggaatc tattgaaggc tcaaacccat
    10981 actgacgagc ccaaaggttc gttttgcgtt ttatgtttcg gttcgatgcc aacgccacat
    11041 tctgagctag gcaaaaaaca aacgtgtctt tgaatagact cctctcgtta acacatgcag
    11101 cggctgcatg gtgacgccat taacacgtgg cctacaattg catgatgtct ccattgacac
    11161 gtgacttctc gtctcctttc ttaatatatc taacaaacac tcctacctct tccaaaatat
    11221 atacacatct ttttgatcaa tctctcattc aaaatctcat tctctctagt aaacaagaac
    11281 aaaaaaatgg cggatacagc tagaggaacc catcacgata tcatcggcag ggaccagtac
    11341 ccgatgatgg gccgtgaccg tgaccagtac cagatgtccg gacgaggatc tgactactcc
    11401 aagtctaggc agattgctaa agctgcaact gctgtcacag ctggtggttc cctccttgtt
    11461 ctctccagcc ttacccttgt tggaactgtc atagctttga ctgttgcaac acctctgctc
    11521 gttatcttca gcccaatcct tgtcccggct ctcatcacag ttgcactcct catcaccggt
    11581 tttctttcct ctggagggtt tggcattgcc gctataaccg ttttctcttg gatttacaag
    11641 taagcacaca tttatcatct tacttcataa ttttgtgcaa tatgtgcatg catgtgttga
    11701 gccagtagct ttggatcaat ttttttggtc gaataacaaa tgtaacaata agaaattgca
    11761 aattctaggg aacatttggt taactaaata cgaaatttga cctagctagc ttgaatgtgt
    11821 ctgtgtatat catctatata ggtaaaatgc ttggtatgat acctattgat tgtgaatagg
    11881 tacgcaacgg gagagcaccc acagggatca gacaagttgg acagtgcaag gatgaagttg
    11941 ggaagcaaag ctcaggatct gaaagacaga gctcagtact acggacagca acatactggt
    12001 ggggaacatg accgtgaccg tactcgtggt ggccagcaca ctactatgag cgagctgatt
    12061 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
    12121 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
    12181 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
    12241 agcagaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct
    12301 gagggcttca catgggagag agtcaccaca tacgaggacg gaggtgtgct gaccgctact
    12361 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
    12421 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaagc caacaccgag
    12481 atgctgtatc cagctgacgg aggactggaa ggcagaagcg acatggccct gaagctcgtg
    12541 ggcgggggcc acctgatctg caacttcaag accacataca gatccaagaa acccgctaag
    12601 aacctcaaga tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc
    12661 gacaaagaaa cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
    12721 agcaaactgg ggcacaagtg attagagttt ttcagatccg cgtttgtgtt ttctgggttt
    12781 ctcacttaag cgtctgcgtt ttacttttgt attgggtttg gcgtttagta gtttgcggta
    12841 gcgttcttgt tatgtgtaat tacgcttttt cttcttgctt cagcagtttc ggttgaaata
    12901 taaatcgaat caagtttcac tttatcagcg ttgttttaaa ttttggcatt aaattggtga
    12961 aaattgcttc aattttgtat ctaaatagaa gtgtcctcat tgtcttccgt aatccgtcta
    13021 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
    13081 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
    13141 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccggcaga agatcctttg
    13201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    13261 atgcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
    13321 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg
    13381 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg
    13441 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
    13501 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
    13561 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
    13621 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
    13681 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
    13741 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
    13801 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
    13861 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
    13921 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
    13981 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
    14041 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
    14101 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
    14161 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
    14221 cccatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
    14281 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
    14341 gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
    14401 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
    14461 agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg cccgaattga tcacaggcag
    14521 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
    14581 acccggcagc ttagttgccg ttcttccgat gagcgtcaga ccccgtagaa aagatcaaag
    14641 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    14701 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    14761 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
    14821 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    14881 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    14941 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    15001 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    15061 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    15121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    15181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
