LOCUS       sequence-475893-       15230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A ubiquitous expression in 
            Arabidopsis plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 15230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: proUBI10::VIH2-FL::FLAG-K379A-K381A-K498A
FEATURES             Location/Qualifiers
     source          1..15230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9674..9685)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10162..10227
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      10235..10487
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13615..13639
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14017..14808)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgactgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gtccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagat gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agaaggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aaccagagaa gacaacagag cggagtctag ctcaacagag cttttaaccc
     5041 aaattggtac aatagaatac aactttagat cataattctc aaaagaaaga gattccttag
     5101 ctattctatc tgccactcca tttccttctc ggcttgtatg cacaagcata aaatcctcaa
     5161 acttgctaag tagatacttt atgtcttgga taattggatt gagacttgac aagcataact
     5221 ttcatgtaac caaagacaca agttgctgag aatccacctc aaaaatgatc ttcctataat
     5281 tgaatcggga taatgacagc acagcccatc taagagcctc cacttctact tccagcacgc
     5341 ttcttacttt taccacagct cttgcaccta accataacac cttccctgta tgatcgcgaa
     5401 gcacccaccc taagccacat tttaatcctt ctgttggcca tgccccatca aagttgcact
     5461 taacccaaga ttgtggtgga gcttcccatg tttctcgtct gtcccgacgg tgttgtggtt
     5521 ggtgctttcc ttacattctg agcctctttc cttctaatcc actcatctgc atcttcttgt
     5581 gtccttacta atacctcatt ggttccaaat tccctccctt taagcaccag ctcgtttctg
     5641 ttcttccaca gcctcccaag tatccaaggg actaaagcct ccacattctt cagatcagga
     5701 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg
     5761 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt
     5821 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg
     5881 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc
     5941 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat
     6001 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg
     6061 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
     6121 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
     6181 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
     6241 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
     6301 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg
     6361 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg
     6421 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa
     6481 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct
     6541 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata
     6601 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta
     6661 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc
     6721 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga
     6781 ttctgttaat cttagatcga atacgatttt ctgggtttga tcgttagata tcatcttaat
     6841 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
     6901 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
     6961 tcgaatttgt cgattaatct gagtttttct gattaacagt ctggaagcac catggagatg
     7021 gaagaaggag caagtggtgt tggtgagaag ataaagattg gagtctgcgt catggaaaag
     7081 aaggttttct cagctcccat gggggaaatt ctcgacagac tccagtcttt tggtgaattt
     7141 gagatcttac attttgggga taaagttata cttgaagatc caatagaaag ttggcccatt
     7201 tgtgattgct tgattgcttt tcattcctct ggatatcctc ttgagaaggc tcaggcatat
     7261 gctgctttaa gaaagccatt tttagtgaat gaacttgatc cgcaatatct tcttcatgat
     7321 cgccggaagg tgtatgagca tcttgagatg tatggcatcc cagttcctag gtatgcttgt
     7381 gtcaatagaa aggtaccaaa tcaagacctt cattattttg tcgaggaaga agattttgtt
     7441 gaggtccatg gtgaacgctt ctggaagcca tttgtggaaa agcctgtcaa tggagatgac
     7501 catagtataa tgatatacta ccctagctcg gcaggtggag gcatgaaaga attgtttcgc
     7561 aagattggga accgatcaag tgaatttcat cctgacgtca gaagggtaag gagagaaggc
     7621 tcttatatat acgaggagtt tatggccacc ggaggaactg atgtcaaggt ctatacagtg
     7681 ggtcctgaat acgcacatgc tgaagcaaga aagtcacctg ttgttgatgg tgttgttatg
     7741 aggaatacag atgggaagga agtgagatat ccagtattgc ttacacctgc tgaaaagcaa
     7801 atggctagag aagtttgcat tgcatttagg caagcggtct gtgggtttga tcttctacga
     7861 tctgagggct gttcatatgt ttgtgatgtc aatggatgga gttttgtgaa gaactcatac
     7921 aagtattatg acgatgctgc gtgtgtgcta aggaaaatgt gtttggatgc aaaagctcct
     7981 catctctcat ccactcttcc tcccaccttg ccatggaagg tcaatgaacc tgtacaatct
     8041 aatgaaggtc taactcgcca gggaagtggc atcatcggca cttttgggca gtcagaagag
     8101 ctacgttgtg tcattgctgt tgttcgacat ggcgatagaa ctcccgcagt ggcactaaaa
     8161 gttacagagg aaaaactatt aaacctaatg ttgaagtaca atggtggaaa gccaagagct
     8221 gaaaccaaac ttaagagtgc cgtccagttg caagatctat tagatgccac aagaatgtta
     8281 gttccccgta caagaccagg tcgtgaaagt gatagtgatg cagaggacct tgaacatgct
     8341 gagaagcttc ggcaagttaa agcagtcctt gaagagggag gacatttctc tggtatatac
     8401 aggaaggttc aactgaagcc gctgaagtgg gttaaaatac caaaaagcga tggtgatggc
     8461 gaagaagaaa gaccagtaga ggcccttatg gttctggcat atggcggtgt tttaacacac
     8521 gctggtagaa agcaggcaga agaacttggt agatactttc gaaacaatat gtatccaggt
     8581 gaagggactg gtttgcttcg tctccatagt acgtaccgtc atgaccttaa aatttacagc
     8641 tctgacgagg gacgtgttca gatgtctgca gcggcttttg ctaaaggcct gcttgaccta
     8701 gaaggacagc tgacgccaat cctggtttct ttggttagca aggactcttc catgttggat
     8761 ggtcttgata atgccagcat tgaaatggaa gcggccaagg ctagattgaa tgagattgta
     8821 acgtctggca caaagatgat agacgacgac caagtctcct ctgaagattt cccttggatg
     8881 actgatggag ctggacttcc tcccaacgct catgaactcc tacgtgaact ggtgaaatta
     8941 actaagaatg tgactgaaca agtaagacta cttgcaatgg atgaggacga gaacctcact
     9001 gagccatacg atataattcc tccatatgat caagcaaaag cccttggcaa gacaaacatt
     9061 gacagtgatc ggattgcttc tggattacca tgtggtagtg aaggattcct tctgatgttt
     9121 gctcggtgga taaaacttgc tagggatctc tacaatgaaa gaaaagaccg atttgacatc
     9181 acacagattc cggatgttta cgattcatgc aagtacgacc tgttacataa ttcccatctc
     9241 gatctaaaag gattagatga actcttcaaa gtagcacagt tacttgcaga tggtgtaatc
     9301 ccaaatgagt atggcatcaa tccgcaacaa aagcttaaaa tcggttcaaa gattgctcgg
     9361 cgcttaatgg ggaaaatctt gatagacttg aggaatactc gagaagaagc actgagcgtt
     9421 gccgagttga aagaaagcca agaacaagtc ttgtcattat ccgcctcgca aagggaagat
     9481 agaaatagtc aaccgaagct atttatcaat agcgatgaat tgagacgacc tggcacaggt
     9541 gataaagatg aagatgatga taaagaaacc aaataccgat tagatccaaa gtatgcaaat
     9601 gtgaaaacac ctgaacgtca tgtgaggact cgactttact tcacatctga atcgcatatt
     9661 cattcgctca tgaacgtcct tcgatactgt aaccttgatg aatctctcct aggagaagaa
     9721 agcctcattt gccaaaacgc cttagaacgt ctctgtaaaa ccaaggaact cgattacatg
     9781 agttacattg tcctaaggct ctttgagaac accgaggtat cacttgagga cccgaaaaga
     9841 ttccgcattg aacttacatt tagccgtgga gctgatttgt ctcccttaag gaataatgac
     9901 gacgaggcag agacattgct aagggaacac acacttccga taatgggacc agagaggctt
     9961 caagaagtag gttcttgttt aagcttggag acaatggaga aaatggttcg tccatttgct
    10021 atgccggcgg aagatttccc tccggcgtcc actccggttg gtttctcggg ttacttctca
    10081 aaaagtgctg cggtgctgga gcgactcgta aacctcttcc acaactataa gaactcttct
    10141 tctaatggaa ggagcaaggg agattacaaa gaccatgatg gagattataa ggatcacgat
    10201 atcgactaca aagatgacga cgataaataa aatcgatcgt tcaaacattt ggcaataaag
    10261 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
    10321 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
    10381 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
    10441 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatctga ggtatgtagg
    10501 tatagtaaca tgaacaagaa cagactcaag tacaagatcg catacgaaaa tggaaatggc
    10561 aatgtcactt ccacataatc aaacacgaat cctcatatca acaaggcctg agattctaac
    10621 tagctcataa caacttagcc aatagttact tgagactacc aaatgtatgt agaactaaag
    10681 actaagggac agagagttcg tctaaacagg tgaatctagt cgttgttatc taataaacaa
    10741 ttcagcccca aatgcagaac acacatagag ctctctattg attcaaatta cgatctgata
    10801 ctgataacgt ctagattttt agggttaaag caatcaatca cctgacgatt caaggtggtt
    10861 ggatcatgac gattccagaa aacatcaagc aagctctcaa agctacactc tttgggatca
    10921 tactgaactc taacaacctc gttatgtccc gtagtgccag tacagacatc ctcgtaactc
    10981 ggattgtgca cgatgccatg gctataccca acctcggtct tggtcacacc aggaactctc
    11041 tggtaagcta gctccactcc ccagaaacaa ccggcgccaa attgcgcgaa ttgctgacct
    11101 gatgacggaa catcatcgtc gggtccttgg gcgattgcgg cggaagatgg gtcagcttgg
    11161 gcttgaggac gagagccgaa tccgagtctg ttgaaaaggt tgttcattgg ggatttgtat
    11221 acggagattg gtcgtcgaga ggtttgaggg aaaggacaaa tgggtttggc tctggagaaa
    11281 gagagtgcgg ctttagagag agaattgaga ggtttagaga gagatgcggc ggcgatgagc
    11341 gtgccgagga gagacgacga ggacctgcat tatcaaagca gtgacgtggt gaaatttgga
    11401 acttttaaga ggcagataga tttattattt gtatccattt tcttcattgt tctagaatgt
    11461 cgcggaacaa attttaaaac taaatcctaa atttttctaa ttttgttgcc aatagtggat
    11521 atgtgggccg tatagaagga atctattgaa ggctcaaacc catactgacg agcccaaagg
    11581 ttcgttttgc gttttatgtt tcggttcgat gccaacgcca cattctgagc taggcaaaaa
    11641 acaaacgtgt ctttgaatag actcctctcg ttaacacatg cagcggctgc atggtgacgc
    11701 cattaacacg tggcctacaa ttgcatgatg tctccattga cacgtgactt ctcgtctcct
    11761 ttcttaatat atctaacaaa cactcctacc tcttccaaaa tatatacaca tctttttgat
    11821 caatctctca ttcaaaatct cattctctct agtaaacaag aacaaaaaaa tggcggatac
    11881 agctagagga acccatcacg atatcatcgg cagggaccag tacccgatga tgggccgtga
    11941 ccgtgaccag taccagatgt ccggacgagg atctgactac tccaagtcta ggcagattgc
    12001 taaagctgca actgctgtca cagctggtgg ttccctcctt gttctctcca gccttaccct
    12061 tgttggaact gtcatagctt tgactgttgc aacacctctg ctcgttatct tcagcccaat
    12121 ccttgtcccg gctctcatca cagttgcact cctcatcacc ggttttcttt cctctggagg
    12181 gtttggcatt gccgctataa ccgttttctc ttggatttac aagtaagcac acatttatca
    12241 tcttacttca taattttgtg caatatgtgc atgcatgtgt tgagccagta gctttggatc
    12301 aatttttttg gtcgaataac aaatgtaaca ataagaaatt gcaaattcta gggaacattt
    12361 ggttaactaa atacgaaatt tgacctagct agcttgaatg tgtctgtgta tatcatctat
    12421 ataggtaaaa tgcttggtat gatacctatt gattgtgaat aggtacgcaa cgggagagca
    12481 cccacaggga tcagacaagt tggacagtgc aaggatgaag ttgggaagca aagctcagga
    12541 tctgaaagac agagctcagt actacggaca gcaacatact ggtggggaac atgaccgtga
    12601 ccgtactcgt ggtggccagc acactactat gagcgagctg attaaggaga acatgcacat
    12661 gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga
    12721 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
    12781 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa ccttcatcaa
    12841 ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga
    12901 gagagtcacc acatacgagg acggaggtgt gctgaccgct actcaggaca ccagcctcca
    12961 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat ccaacggccc
    13021 tgtgatgcag aagaaaacac tcggctggga agccaacacc gagatgctgt atccagctga
    13081 cggaggactg gaaggcagaa gcgacatggc cctgaagctc gtgggcgggg gccacctgat
    13141 ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca agatgcccgg
    13201 cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag aaacctacgt
    13261 cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac tggggcacaa
    13321 gtgattagag tttttcagat ccgcgtttgt gttttctggg tttctcactt aagcgtctgc
    13381 gttttacttt tgtattgggt ttggcgttta gtagtttgcg gtagcgttct tgttatgtgt
    13441 aattacgctt tttcttcttg cttcagcagt ttcggttgaa atataaatcg aatcaagttt
    13501 cactttatca gcgttgtttt aaattttggc attaaattgg tgaaaattgc ttcaattttg
    13561 tatctaaata gaagtgtcct cattgtcttc cgtaatccgt ctaagcgtca atttgtttac
    13621 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
    13681 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
    13741 aaggcggcag actttgctca tgttaccggc agaagatcct ttgatctttt ctacggggtc
    13801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
    13861 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
    13921 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
    13981 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
    14041 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    14101 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
    14161 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
    14221 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
    14281 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
    14341 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
    14401 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
    14461 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
    14521 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
    14581 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
    14641 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
    14701 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
    14761 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
    14821 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
    14881 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
    14941 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
    15001 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
    15061 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
    15121 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    15181 ccgttcttcc gatgagcgtc agaccccgta gaaaagatca aaggatcttc
//
