LOCUS       sequence-475894-       15236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous 
            expression in Arabidopsis plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 15236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A
FEATURES             Location/Qualifiers
     source          1..15236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      273..861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     762..781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1015..1032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1047..1187
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1113..1132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1273..1295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1531..1725
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1791..2864)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3293..3922)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5223..5247
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9952..9963)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10440..10505
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      10513..10765
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13893..13917
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14295..15086)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
       61 tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt
      121 tcgtgcccga attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
      181 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgatgagcg
      241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      661 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      961 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1021 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     1081 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1141 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     1201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1261 ctgtgactgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1321 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     1381 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     1441 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     1501 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     1561 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     1621 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     1681 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagtcctttt
     1741 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     1801 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     1861 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     1921 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     1981 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     2041 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     2101 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     2161 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     2221 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     2281 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     2341 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     2401 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     2461 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     2521 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     2581 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     2641 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     2701 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     2761 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     2821 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
     2881 tgggggttcc agtgccattg cagggccggc aggcaaccca gccgcttacg cctggccaac
     2941 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
     3001 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
     3061 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
     3121 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
     3181 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
     3241 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
     3301 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
     3361 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
     3421 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
     3481 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
     3541 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
     3601 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
     3661 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
     3721 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
     3781 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
     3841 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
     3901 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
     3961 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
     4021 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
     4081 agacaaaccg gccaggattt catgcagccg cacggttgag atgtgcgcgg gcggctcgaa
     4141 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
     4201 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
     4261 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
     4321 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
     4381 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
     4441 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
     4501 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
     4561 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
     4621 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
     4681 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
     4741 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
     4801 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
     4861 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
     4921 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
     4981 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
     5041 gaagaaggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     5101 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
     5161 gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt
     5221 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaaccagag aagacaacag
     5281 agcggagtct agctcaacag agcttttaac ccaaattggt acaatagaat acaactttag
     5341 atcataattc tcaaaagaaa gagattcctt agctattcta tctgccactc catttccttc
     5401 tcggcttgta tgcacaagca taaaatcctc aaacttgcta agtagatact ttatgtcttg
     5461 gataattgga ttgagacttg acaagcataa ctttcatgta accaaagaca caagttgctg
     5521 agaatccacc tcaaaaatga tcttcctata attgaatcgg gataatgaca gcacagccca
     5581 tctaagagcc tccacttcta cttccagcac gcttcttact tttaccacag ctcttgcacc
     5641 taaccataac accttccctg tatgatcgcg aagcacccac cctaagccac attttaatcc
     5701 ttctgttggc catgccccat caaagttgca cttaacccaa gattgtggtg gagcttccca
     5761 tgtttctcgt ctgtcccgac ggtgttgtgg ttggtgcttt ccttacattc tgagcctctt
     5821 tccttctaat ccactcatct gcatcttctt gtgtccttac taatacctca ttggttccaa
     5881 attccctccc tttaagcacc agctcgtttc tgttcttcca cagcctccca agtatccaag
     5941 ggactaaagc ctccacattc ttcagatcag gatattcttg tttaagatgt tgaactctat
     6001 ggaggtttgt atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt
     6061 attcaaagaa tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt
     6121 ggtcattgga ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat
     6181 atatgaatta aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga
     6241 cttgttcacc aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa
     6301 tatccaataa cactaaaaaa ttaaaagaaa tggataattt cacaatatgt tatacgataa
     6361 agaagttact tttccaagaa attcactgat tttataagcc cacttgcatt agataaatgg
     6421 caaaaaaaaa caaaaaggaa aagaaataaa gcacgaagaa ttctagaaaa tacgaaatac
     6481 gcttcaatgc agtgggaccc acggttcaat tattgccaat tttcagctcc accgtatatt
     6541 taaaaaataa aacgataatg ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg
     6601 gctggatctt atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg
     6661 tcaaagtggt tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt
     6721 ttcctcaacc taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca
     6781 atacacgtgt cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag
     6841 tcgtcctcgt cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt
     6901 cacaattcag atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt
     6961 gatcaaggta aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg
     7021 atcccaattt cgtatatgtt ctttggttta gattctgtta atcttagatc gaatacgatt
     7081 ttctgggttt gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc
     7141 cgatttgttc aaataatttg agttttgtcg aataattact cttcgatttg tgatttctat
     7201 ctagatctgg tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt
     7261 ctgattaaca gtctggaagc accatggaga tggaagaagg agcaagtggt gttggtgaga
     7321 agataaagat tggagtctgc gtcatggaaa agaaggtttt ctcagctccc atgggggaaa
     7381 ttctcgacag actccagtct tttggtgaat ttgagatctt acattttggg gataaagtta
     7441 tacttgaaga tccaatagaa agttggccca tttgtgattg cttgattgct tttcattcct
     7501 ctggatatcc tcttgagaag gctcaggcat atgctgcttt aagaaagcca tttttagtga
     7561 atgaacttga tccgcaatat cttcttcatg atcgccggaa ggtgtatgag catcttgaga
     7621 tgtatggcat cccagttcct aggtatgctt gtgtcaatag aaaggtacca aatcaagacc
     7681 ttcattattt tgtcgaggaa gaagattttg ttgaggtcca tggtgaacgc ttctggaagc
     7741 catttgtgga aaagcctgtc aatggagatg accatagtat aatgatatac taccctagct
     7801 cggcaggtgg aggcatgaaa gaattgtttc gcaagattgg gaaccgatca agtgaatttc
     7861 atcctgacgt cagaagggta aggagagaag gctcttatat atacgaggag tttatggcca
     7921 ccggaggaac tgatgtcaag gtctatacag tgggtcctga atacgcacat gctgaagcaa
     7981 gaaagtcacc tgttgttgat ggtgttgtta tgaggaatac agatgggaag gaagtgagat
     8041 atccagtatt gcttacacct gctgaaaagc aaatggctag agaagtttgc attgcattta
     8101 ggcaagcggt ctgtgggttt gatcttctac gatctgaggg ctgttcatat gtttgtgatg
     8161 tcaatggatg gagttttgtg aagaactcat acaagtatta tgacgatgct gcgtgtgtgc
     8221 taaggaaaat gtgtttggat gcaaaagctc ctcatctctc atccactctt cctcccacct
     8281 tgccatggaa ggtcaatgaa cctgtacaat ctaatgaagg tctaactcgc cagggaagtg
     8341 gcatcatcgg cacttttggg cagtcagaag agctacgttg tgtcattgct gttgttgcag
     8401 caggcgatgc aactcccaag cagaaagtga aactaaaagt tacagaggaa aaactattaa
     8461 acctaatgtt gaagtacaat ggtggaaagc caagagctga aaccaaactt aagagtgccg
     8521 tccagttgca agatctatta gatgccacaa gaatgttagt tccccgtaca agaccaggtc
     8581 gtgaaagtga tagtgatgca gaggaccttg aacatgctga gaagcttcgg caagttaaag
     8641 cagtccttga agagggagga catttctctg gtatatacag gaaggttcaa ctgaagccgc
     8701 tgaagtgggt taaaatacca aaaagcgatg gtgatggcga agaagaaaga ccagtagagg
     8761 cccttatggt tctgaaatat ggcggtgttt taacacacgc tggtagaaag caggcagaag
     8821 aacttggtag atactttcga aacaatatgt atccaggtga agggactggt ttgcttcgtc
     8881 tccatagtac gtaccgtcat gaccttaaaa tttacagctc tgacgaggga cgtgttcaga
     8941 tgtctgcagc ggcttttgct aaaggcctgc ttgacctaga aggacagctg acgccaatcc
     9001 tggtttcttt ggttagcaag gactcttcca tgttggatgg tcttgataat gccagcattg
     9061 aaatggaagc ggccaaggct agattgaatg agattgtaac gtctggcaca aagatgatag
     9121 acgacgacca agtctcctct gaagatttcc cttggatgac tgatggagct ggacttcctc
     9181 ccaacgctca tgaactccta cgtgaactgg tgaaattaac taagaatgtg actgaacaag
     9241 taagactact tgcaatggat gaggacgaga acctcactga gccatacgat ataattcctc
     9301 catatgatca agcaaaagcc cttggcaaga caaacattga cagtgatcgg attgcttctg
     9361 gattaccatg tggtagtgaa ggattccttc tgatgtttgc tcggtggata aaacttgcta
     9421 gggatctcta caatgaaaga aaagaccgat ttgacatcac acagattccg gatgtttacg
     9481 attcatgcaa gtacgacctg ttacataatt cccatctcga tctaaaagga ttagatgaac
     9541 tcttcaaagt agcacagtta cttgcagatg gtgtaatccc aaatgagtat ggcatcaatc
     9601 cgcaacaaaa gcttaaaatc ggttcaaaga ttgctcggcg cttaatgggg aaaatcttga
     9661 tagacttgag gaatactcga gaagaagcac tgagcgttgc cgagttgaaa gaaagccaag
     9721 aacaagtctt gtcattatcc gcctcgcaaa gggaagatag aaatagtcaa ccgaagctat
     9781 ttatcaatag cgatgaattg agacgacctg gcacaggtga taaagatgaa gatgatgata
     9841 aagaaaccaa ataccgatta gatccaaagt atgcaaatgt gaaaacacct gaacgtcatg
     9901 tgaggactcg actttacttc acatctgcat cgcatattca ttcgctcatg aacgtccttc
     9961 gatactgtaa ccttgatgaa tctctcctag gagaagaaag cctcatttgc caaaacgcct
    10021 tagaacgtct ctgtaaaacc aaggaactcg attacatgag ttacattgtc ctaaggctct
    10081 ttgagaacac cgaggtatca cttgaggacc cgaaaagatt ccgcattgaa cttacattta
    10141 gccgtggagc tgatttgtct cccttaagga ataatgacga cgaggcagag acattgctaa
    10201 gggaacacac acttccgata atgggaccag agaggcttca agaagtaggt tcttgtttaa
    10261 gcttggagac aatggagaaa atggttcgtc catttgctat gccggcggaa gatttccctc
    10321 cggcgtccac tccggttggt ttctcgggtt acttctcaaa aagtgctgcg gtgctggagc
    10381 gactcgtaaa cctcttccac aactataaga actcttcttc taatggaagg agcaagggag
    10441 attacaaaga ccatgatgga gattataagg atcacgatat cgactacaaa gatgacgacg
    10501 ataaataaaa tcgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
    10561 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
    10621 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
    10681 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
    10741 gcggtgtcat ctatgttact agatctgagg tatgtaggta tagtaacatg aacaagaaca
    10801 gactcaagta caagatcgca tacgaaaatg gaaatggcaa tgtcacttcc acataatcaa
    10861 acacgaatcc tcatatcaac aaggcctgag attctaacta gctcataaca acttagccaa
    10921 tagttacttg agactaccaa atgtatgtag aactaaagac taagggacag agagttcgtc
    10981 taaacaggtg aatctagtcg ttgttatcta ataaacaatt cagccccaaa tgcagaacac
    11041 acatagagct ctctattgat tcaaattacg atctgatact gataacgtct agatttttag
    11101 ggttaaagca atcaatcacc tgacgattca aggtggttgg atcatgacga ttccagaaaa
    11161 catcaagcaa gctctcaaag ctacactctt tgggatcata ctgaactcta acaacctcgt
    11221 tatgtcccgt agtgccagta cagacatcct cgtaactcgg attgtgcacg atgccatggc
    11281 tatacccaac ctcggtcttg gtcacaccag gaactctctg gtaagctagc tccactcccc
    11341 agaaacaacc ggcgccaaat tgcgcgaatt gctgacctga tgacggaaca tcatcgtcgg
    11401 gtccttgggc gattgcggcg gaagatgggt cagcttgggc ttgaggacga gagccgaatc
    11461 cgagtctgtt gaaaaggttg ttcattgggg atttgtatac ggagattggt cgtcgagagg
    11521 tttgagggaa aggacaaatg ggtttggctc tggagaaaga gagtgcggct ttagagagag
    11581 aattgagagg tttagagaga gatgcggcgg cgatgagcgt gccgaggaga gacgacgagg
    11641 acctgcatta tcaaagcagt gacgtggtga aatttggaac ttttaagagg cagatagatt
    11701 tattatttgt atccattttc ttcattgttc tagaatgtcg cggaacaaat tttaaaacta
    11761 aatcctaaat ttttctaatt ttgttgccaa tagtggatat gtgggccgta tagaaggaat
    11821 ctattgaagg ctcaaaccca tactgacgag cccaaaggtt cgttttgcgt tttatgtttc
    11881 ggttcgatgc caacgccaca ttctgagcta ggcaaaaaac aaacgtgtct ttgaatagac
    11941 tcctctcgtt aacacatgca gcggctgcat ggtgacgcca ttaacacgtg gcctacaatt
    12001 gcatgatgtc tccattgaca cgtgacttct cgtctccttt cttaatatat ctaacaaaca
    12061 ctcctacctc ttccaaaata tatacacatc tttttgatca atctctcatt caaaatctca
    12121 ttctctctag taaacaagaa caaaaaaatg gcggatacag ctagaggaac ccatcacgat
    12181 atcatcggca gggaccagta cccgatgatg ggccgtgacc gtgaccagta ccagatgtcc
    12241 ggacgaggat ctgactactc caagtctagg cagattgcta aagctgcaac tgctgtcaca
    12301 gctggtggtt ccctccttgt tctctccagc cttacccttg ttggaactgt catagctttg
    12361 actgttgcaa cacctctgct cgttatcttc agcccaatcc ttgtcccggc tctcatcaca
    12421 gttgcactcc tcatcaccgg ttttctttcc tctggagggt ttggcattgc cgctataacc
    12481 gttttctctt ggatttacaa gtaagcacac atttatcatc ttacttcata attttgtgca
    12541 atatgtgcat gcatgtgttg agccagtagc tttggatcaa tttttttggt cgaataacaa
    12601 atgtaacaat aagaaattgc aaattctagg gaacatttgg ttaactaaat acgaaatttg
    12661 acctagctag cttgaatgtg tctgtgtata tcatctatat aggtaaaatg cttggtatga
    12721 tacctattga ttgtgaatag gtacgcaacg ggagagcacc cacagggatc agacaagttg
    12781 gacagtgcaa ggatgaagtt gggaagcaaa gctcaggatc tgaaagacag agctcagtac
    12841 tacggacagc aacatactgg tggggaacat gaccgtgacc gtactcgtgg tggccagcac
    12901 actactatga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
    12961 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
    13021 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
    13081 gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg catccccgac
    13141 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaggac
    13201 ggaggtgtgc tgaccgctac tcaggacacc agcctccagg acggctgcct catctacaac
    13261 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
    13321 ggctgggaag ccaacaccga gatgctgtat ccagctgacg gaggactgga aggcagaagc
    13381 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa gaccacatac
    13441 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggaccacaga
    13501 ctggaaagaa tcaaggaggc cgacaaagaa acctacgtcg agcagcacga ggtggctgtg
    13561 gccagatact gcgacctccc tagcaaactg gggcacaagt gattagagtt tttcagatcc
    13621 gcgtttgtgt tttctgggtt tctcacttaa gcgtctgcgt tttacttttg tattgggttt
    13681 ggcgtttagt agtttgcggt agcgttcttg ttatgtgtaa ttacgctttt tcttcttgct
    13741 tcagcagttt cggttgaaat ataaatcgaa tcaagtttca ctttatcagc gttgttttaa
    13801 attttggcat taaattggtg aaaattgctt caattttgta tctaaataga agtgtcctca
    13861 ttgtcttccg taatccgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
    13921 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
    13981 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
    14041 ttaccggcag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    14101 tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat
    14161 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt
    14221 gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa
    14281 tttctagcta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
    14341 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
    14401 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
    14461 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
    14521 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
    14581 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
    14641 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
    14701 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
    14761 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
    14821 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
    14881 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
    14941 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
    15001 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
    15061 acttcggcga tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc
    15121 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
    15181 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccat
//
