LOCUS       sequence-475900-       15235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in 
            Arabidopsis plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 15235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A
FEATURES             Location/Qualifiers
     source          1..15235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1012..1803
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2181..2205
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(5333..5584)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5592..5657)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6134..6145
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    10850..10874
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12175..12804
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13233..14306
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14372..14566
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14802..14824)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14910..15050
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14965..14984
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15065..15082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcatcg gaagaacggc aactaagctg ccgggtttga
      661 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
      721 ctgtgatcaa ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
      781 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      841 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
      901 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      961 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     1021 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1081 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1141 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1201 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1261 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1321 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1381 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1441 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1501 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1561 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1621 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1681 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     1741 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     1801 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     1861 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     1921 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc
     1981 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2041 ggatcttctg ccggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     2101 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     2161 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     2221 cggattacgg aagacaatga ggacacttct atttagatac aaaattgaag caattttcac
     2281 caatttaatg ccaaaattta aaacaacgct gataaagtga aacttgattc gatttatatt
     2341 tcaaccgaaa ctgctgaagc aagaagaaaa agcgtaatta cacataacaa gaacgctacc
     2401 gcaaactact aaacgccaaa cccaatacaa aagtaaaacg cagacgctta agtgagaaac
     2461 ccagaaaaca caaacgcgga tctgaaaaac tctaatcact tgtgccccag tttgctaggg
     2521 aggtcgcagt atctggccac agccacctcg tgctgctcga cgtaggtttc tttgtcggcc
     2581 tccttgattc tttccagtct gtggtccaca tagtagacgc cgggcatctt gaggttctta
     2641 gcgggtttct tggatctgta tgtggtcttg aagttgcaga tcaggtggcc cccgcccacg
     2701 agcttcaggg ccatgtcgct tctgccttcc agtcctccgt cagctggata cagcatctcg
     2761 gtgttggctt cccagccgag tgttttcttc tgcatcacag ggccgttgga tgggaagttc
     2821 acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgagta
     2881 gcggtcagca cacctccgtc ctcgtatgtg gtgactctct cccatgtgaa gccctcaggg
     2941 aaggactgct taaagaagtc ggggatgccc tgggtgtggt tgatgaaggt tctgctgccg
     3001 tacatgaagc tggtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc
     3061 ttgattctca tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg
     3121 aagtggtggt tgttcacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc
     3181 agctcgctca tagtagtgtg ctggccacca cgagtacggt cacggtcatg ttccccacca
     3241 gtatgttgct gtccgtagta ctgagctctg tctttcagat cctgagcttt gcttcccaac
     3301 ttcatccttg cactgtccaa cttgtctgat ccctgtgggt gctctcccgt tgcgtaccta
     3361 ttcacaatca ataggtatca taccaagcat tttacctata tagatgatat acacagacac
     3421 attcaagcta gctaggtcaa atttcgtatt tagttaacca aatgttccct agaatttgca
     3481 atttcttatt gttacatttg ttattcgacc aaaaaaattg atccaaagct actggctcaa
     3541 cacatgcatg cacatattgc acaaaattat gaagtaagat gataaatgtg tgcttacttg
     3601 taaatccaag agaaaacggt tatagcggca atgccaaacc ctccagagga aagaaaaccg
     3661 gtgatgagga gtgcaactgt gatgagagcc gggacaagga ttgggctgaa gataacgagc
     3721 agaggtgttg caacagtcaa agctatgaca gttccaacaa gggtaaggct ggagagaaca
     3781 aggagggaac caccagctgt gacagcagtt gcagctttag caatctgcct agacttggag
     3841 tagtcagatc ctcgtccgga catctggtac tggtcacggt cacggcccat catcgggtac
     3901 tggtccctgc cgatgatatc gtgatgggtt cctctagctg tatccgccat ttttttgttc
     3961 ttgtttacta gagagaatga gattttgaat gagagattga tcaaaaagat gtgtatatat
     4021 tttggaagag gtaggagtgt ttgttagata tattaagaaa ggagacgaga agtcacgtgt
     4081 caatggagac atcatgcaat tgtaggccac gtgttaatgg cgtcaccatg cagccgctgc
     4141 atgtgttaac gagaggagtc tattcaaaga cacgtttgtt ttttgcctag ctcagaatgt
     4201 ggcgttggca tcgaaccgaa acataaaacg caaaacgaac ctttgggctc gtcagtatgg
     4261 gtttgagcct tcaatagatt ccttctatac ggcccacata tccactattg gcaacaaaat
     4321 tagaaaaatt taggatttag ttttaaaatt tgttccgcga cattctagaa caatgaagaa
     4381 aatggataca aataataaat ctatctgcct cttaaaagtt ccaaatttca ccacgtcact
     4441 gctttgataa tgcaggtcct cgtcgtctct cctcggcacg ctcatcgccg ccgcatctct
     4501 ctctaaacct ctcaattctc tctctaaagc cgcactctct ttctccagag ccaaacccat
     4561 ttgtcctttc cctcaaacct ctcgacgacc aatctccgta tacaaatccc caatgaacaa
     4621 ccttttcaac agactcggat tcggctctcg tcctcaagcc caagctgacc catcttccgc
     4681 cgcaatcgcc caaggacccg acgatgatgt tccgtcatca ggtcagcaat tcgcgcaatt
     4741 tggcgccggt tgtttctggg gagtggagct agcttaccag agagttcctg gtgtgaccaa
     4801 gaccgaggtt gggtatagcc atggcatcgt gcacaatccg agttacgagg atgtctgtac
     4861 tggcactacg ggacataacg aggttgttag agttcagtat gatcccaaag agtgtagctt
     4921 tgagagcttg cttgatgttt tctggaatcg tcatgatcca accaccttga atcgtcaggt
     4981 gattgattgc tttaacccta aaaatctaga cgttatcagt atcagatcgt aatttgaatc
     5041 aatagagagc tctatgtgtg ttctgcattt ggggctgaat tgtttattag ataacaacga
     5101 ctagattcac ctgtttagac gaactctctg tcccttagtc tttagttcta catacatttg
     5161 gtagtctcaa gtaactattg gctaagttgt tatgagctag ttagaatctc aggccttgtt
     5221 gatatgagga ttcgtgtttg attatgtgga agtgacattg ccatttccat tttcgtatgc
     5281 gatcttgtac ttgagtctgt tcttgttcat gttactatac ctacatacct cagatctagt
     5341 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     5401 atcgcgtatt aaattataat tgcgggactc taatcataaa aacccatctc ataaataacg
     5461 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
     5521 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
     5581 gatcgatttt atttatcgtc gtcatctttg tagtcgatat cgtgatcctt ataatctcca
     5641 tcatggtctt tgtaatctcc cttgctcctt ccattagaag aagagttctt atagttgtgg
     5701 aagaggttta cgagtcgctc cagcaccgca gcactttttg agaagtaacc cgagaaacca
     5761 accggagtgg acgccggagg gaaatcttcc gccggcatag caaatggacg aaccattttc
     5821 tccattgtct ccaagcttaa acaagaacct acttcttgaa gcctctctgg tcccattatc
     5881 ggaagtgtgt gttcccttag caatgtctct gcctcgtcgt cattattcct taagggagac
     5941 aaatcagctc cacggctaaa tgtaagttca atgcggaatc ttttcgggtc ctcaagtgat
     6001 acctcggtgt tctcaaagag ccttaggaca atgtaactca tgtaatcgag ttccttggtt
     6061 ttacagagac gttctaaggc gttttggcaa atgaggcttt cttctcctag gagagattca
     6121 tcaaggttac agtatcgaag gacgttcatg agcgaatgaa tatgcgattc agatgtgaag
     6181 taaagtcgag tcctcacatg acgttcaggt gttttcacat ttgcatactt tggatctaat
     6241 cggtatttgg tttctttatc atcatcttca tctttatcac ctgtgccagg tcgtctcaat
     6301 tcatcgctat tgataaatag cttcggttga ctatttctat cttccctttg cgaggcggat
     6361 aatgacaaga cttgttcttg gctttctttc aactcggcaa cgctcagtgc ttcttctcga
     6421 gtattcctca agtctatcaa gattttcccc attaagcgcc gagcaatctt tgaaccgatt
     6481 ttaagctttt gttgcggatt gatgccatac tcatttggga ttacaccatc tgcaagtaac
     6541 tgtgctactt tgaagagttc atctaatcct tttagatcga gatgggaatt atgtaacagg
     6601 tcgtacttgc atgaatcgta aacatccgga atctgtgcga ttgcaaagct gtcttttctt
     6661 tcattgtaga gatccctagc aagttttatc caccgagcaa acatcagaag gaatccttca
     6721 ctaccacatg gtaatccaga agcaatccga tcactgtcaa tgtttgtctt gccaagggct
     6781 tttgcttgat catatggagg aattatatcg tatggctcag tgaggttctc gtcctcatcc
     6841 attgcaagta gtcttacttg ttcagtcaca ttcttagtta atttcaccag ttcacgtagg
     6901 agttcatgag cgttgggagg aagtccagct ccatcagtca tccaagggaa atcttcagag
     6961 gagacttggt cgtcgtctat catctttgtg ccagacgtta caatctcatt caatctagcc
     7021 ttggccgctt ccatttcaat gctggcatta tcaagaccat ccaacatgga agagtccttg
     7081 ctaaccaaag aaaccaggat tggcgtcagc tgtccttcta ggtcaagcag gcctttagca
     7141 aaagccgctg cagacatctg aacacgtccc tcgtcagagc tgtaaatttt aaggtcatga
     7201 cggtacgtac tatggagacg aagcaaacca gtcccttcac ctggatacat attgtttcga
     7261 aagtatctac caagttcttc tgcctgcttt ctaccagcgt gtgttaaaac accgccatat
     7321 ttcagaacca taagggcctc tactggtctt tcttcttcgc catcaccatc gctttttggt
     7381 attttaaccc acttcagcgg cttcagttga accttcctgt atataccaga gaaatgtcct
     7441 ccctcttcaa ggactgcttt aacttgccga agcttctcag catgttcaag gtcctctgca
     7501 tcactatcac tttcacgacc tggtcttgta cggggaacta acattcttgt ggcatctaat
     7561 agatcttgca actggacggc actcttaagt ttggtttcag ctcttggctt tccaccattg
     7621 tacttcaaca ttaggtttaa tagtttttcc tctgtaactt ttagtttcac tttctgcttg
     7681 ggagttctat cgccatgtcg aacaacagca atgacacaac gtagctcttc tgactgccca
     7741 aaagtgccga tgatgccact tccctggcga gttagacctt cattagattg tacaggttca
     7801 ttgaccttcc atggcaaggt gggaggaaga gtggatgaga gatgaggagc ttttgcatcc
     7861 aaacacattt tccttagcac acacgcagca tcgtcataat acttgtatga gttcttcaca
     7921 aaactccatc cattgacatc acaaacatat gaacagccct cagatcgtag aagatcaaac
     7981 ccacagaccg cttgcctaaa tgcaatgcaa acttctctag ccatttgctt ttcagcaggt
     8041 gtaagcaata ctggatatct cacttccttc ccatctgtat tcctcataac aacaccatca
     8101 acaacaggtg actttcttgc ttcagcatgt gcgtattcag gacccactgt atagaccttg
     8161 acatcagttc ctccggtggc cataaactcc tcgtatatat aagagccttc tctccttacc
     8221 cttctgacgt caggatgaaa ttcacttgat cggttcccaa tcttgcgaaa caattctttc
     8281 atgcctccac ctgccgagct agggtagtat atcattatac tatggtcatc tccattgaca
     8341 ggcttttcca caaatggctt ccagaagcgt tcaccatgga cctcaacaaa atcttcttcc
     8401 tcgacaaaat aatgaaggtc ttgatttggt acctttctat tgacacaagc atacctagga
     8461 actgggatgc catacatctc aagatgctca tacaccttcc ggcgatcatg aagaagatat
     8521 tgcggatcaa gttcattcac taaaaatggc tttcttaaag cagcatatgc ctgagccttc
     8581 tcaagaggat atccagagga atgaaaagca atcaagcaat cacaaatggg ccaactttct
     8641 attggatctt caagtataac tttatcccca aaatgtaaga tctcaaattc accaaaagac
     8701 tggagtctgt cgagaatttc ccccatggga gctgagaaaa ccttcttttc catgacgcag
     8761 actccaatct ttatcttctc accaacacca cttgctcctt cttccatctc catggtgctt
     8821 ccagactgtt aatcagaaaa actcagatta atcgacaaat tcgatcgcac aaactagaaa
     8881 ctaacaccag atctagatag aaatcacaaa tcgaagagta attattcgac aaaactcaaa
     8941 ttatttgaac aaatcggatg atatctatga aaccctaatc gagaattaag atgatatcta
     9001 acgatcaaac ccagaaaatc gtattcgatc taagattaac agaatctaaa ccaaagaaca
     9061 tatacgaaat tgggatcgaa cgaaaacaaa atcgaagatt ttgagagaat aaggaacaca
     9121 gaaatttacc ttgatcacgg tagagagaat tgagagaaag tttttaagat tttgagaaat
     9181 tgaaatctga attgtgaaga agaagagctc tttgggtatt gttttataga agaagaagaa
     9241 gaaaagacga ggacgactag gtcacgagaa agctaaggcg gtgaagcaat agctaataat
     9301 aaaatgacac gtgtattgag cgttgtttac acgcaaagtt gtttttggct aattgcctta
     9361 tttttaggtt gaggaaaagt atttgtgctt tgagttgata aacacgactc gtgtgtgccg
     9421 gctgcaacca ctttgacgcc gtttattact gactcgtcga caaccacaat ttctaacggt
     9481 cgtcataaga tccagccgtt gagatttaac gatcgttacg atttatattt ttttagcatt
     9541 atcgttttat tttttaaata tacggtggag ctgaaaattg gcaataattg aaccgtgggt
     9601 cccactgcat tgaagcgtat ttcgtatttt ctagaattct tcgtgcttta tttcttttcc
     9661 tttttgtttt tttttgccat ttatctaatg caagtgggct tataaaatca gtgaatttct
     9721 tggaaaagta acttctttat cgtataacat attgtgaaat tatccatttc ttttaatttt
     9781 ttagtgttat tggatatttt tgtatgatta ttgatttgca taggataatg acttttgtat
     9841 caagttggtg aacaagtctc gttaaaaaag gcaagtggtt tggtgactcg atttattctt
     9901 gttatttaat tcatatatca atggatctta tttggggcct ggtccatatt taacactcgt
     9961 gttcagtcca atgaccaata atattttttc attaataaca atgtaacaag aatgatacac
    10021 aaaacattct ttgaataagt tcgctatgaa gaagggaact tatccggtcc tagatcatca
    10081 gttcatacaa acctccatag agttcaacat cttaaacaag aatatcctga tctgaagaat
    10141 gtggaggctt tagtcccttg gatacttggg aggctgtgga agaacagaaa cgagctggtg
    10201 cttaaaggga gggaatttgg aaccaatgag gtattagtaa ggacacaaga agatgcagat
    10261 gagtggatta gaaggaaaga ggctcagaat gtaaggaaag caccaaccac aacaccgtcg
    10321 ggacagacga gaaacatggg aagctccacc acaatcttgg gttaagtgca actttgatgg
    10381 ggcatggcca acagaaggat taaaatgtgg cttagggtgg gtgcttcgcg atcatacagg
    10441 gaaggtgtta tggttaggtg caagagctgt ggtaaaagta agaagcgtgc tggaagtaga
    10501 agtggaggct cttagatggg ctgtgctgtc attatcccga ttcaattata ggaagatcat
    10561 ttttgaggtg gattctcagc aacttgtgtc tttggttaca tgaaagttat gcttgtcaag
    10621 tctcaatcca attatccaag acataaagta tctacttagc aagtttgagg attttatgct
    10681 tgtgcataca agccgagaag gaaatggagt ggcagataga atagctaagg aatctctttc
    10741 ttttgagaat tatgatctaa agttgtattc tattgtacca atttgggtta aaagctctgt
    10801 tgagctagac tccgctctgt tgtcttctct ggtttaaact atcagtgttt gacaggatat
    10861 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
    10921 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
    10981 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
    11041 cagtgcagcc ttcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
    11101 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
    11161 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
    11221 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
    11281 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
    11341 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
    11401 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
    11461 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
    11521 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
    11581 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
    11641 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
    11701 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
    11761 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
    11821 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
    11881 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
    11941 cgcggccggg tacgtgttcg agccgcccgc gcacatctca accgtgcggc tgcatgaaat
    12001 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
    12061 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
    12121 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
    12181 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
    12241 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
    12301 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
    12361 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
    12421 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
    12481 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
    12541 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
    12601 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
    12661 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
    12721 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
    12781 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
    12841 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
    12901 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
    12961 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
    13021 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
    13081 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
    13141 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat
    13201 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
    13261 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
    13321 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
    13381 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
    13441 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
    13501 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
    13561 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
    13621 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
    13681 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
    13741 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
    13801 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
    13861 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
    13921 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
    13981 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
    14041 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
    14101 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
    14161 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
    14221 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
    14281 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
    14341 caggggaaaa aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa
    14401 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
    14461 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
    14521 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
    14581 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
    14641 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
    14701 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
    14761 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
    14821 ccggagacag tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
    14881 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
    14941 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    15001 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
    15061 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    15121 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    15181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtt
//
