LOCUS       sequence-475904-       12026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GAL-inducible peroxisomal sfCherry3C large fragment and 
            Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12026)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 12026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1141
FEATURES             Location/Qualifiers
     source          1..12026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             732..1172
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1218..2153
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1266..2156
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        3458..3899
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3458..3575
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3870..3893
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5674..6384
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        7211..7618
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7619..8713
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(9744..10559)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9744..10559)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10467..10486
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10551..10570
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10784..11372
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11273..11292
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgagtcg aacaagaagc aggcaaagtt tagagcactg
       61 cccctccgca ctcaaaaaag aaaaaactag gaggaaaata aaattctcaa ccacacaaac
      121 acataaacac atacaaatac aaatacaagc ttatttactt gacatcgcgc gatcttccac
      181 tattcagcgc cgtccgccct ctctcgtgtt ttttgtttac gcgacaacta tgcgaaatcc
      241 ggagcaacgg gcaaccgttt ggggaaagac cacacccacg cgcgatcgcc atggcaacga
      301 ggtcgcacac gccccacacc cagacctccc tgcgagcggg catgggtaca atgtccccgt
      361 tgccacagac accacttcgt agcacagcgc agagcgtagc gtgttgttgc tgctgacaaa
      421 agaaaatttt tcttagcaaa gcaaaggagg ggaagcacgg gcagatagca ccgtaccata
      481 cccttggaaa ctcgaaatga acgaagcagg aaatgagaga atgagagttt tgtaggtata
      541 tatagcggta gtgtttgcgc gttaccatca tcttctggat ctatctattg ttcttttcct
      601 catcactttc ccctttttcg ctcttcttct tgtcttttat ttctttcttt tttttaattg
      661 ttccctcgat tggctatcta ccaaagaatc caaacttaat acacgtattt atttgtccaa
      721 ttaccagatc tatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
      781 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
      841 agtgtcgcta ctctcccaaa accaaaagat ctccgctgac tagggcacat ctgacagaag
      901 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
      961 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     1021 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     1081 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     1141 gtaacaaagg tcaaagacag ttgactgtat cgattgactc ggcaggatcc ggtgacggtg
     1201 ctggtttaat taactctgct ggagacatga gagctgccaa cctttggcca agcccgctca
     1261 tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca
     1321 gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct
     1381 tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca
     1441 tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc
     1501 accttctaga atgtgcctgg ctagagatcc tgatgattgg actcgtctgg cgctccatgg
     1561 agcacccagg gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat
     1621 gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca
     1681 tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg
     1741 gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc
     1801 gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc
     1861 tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca
     1921 tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct
     1981 atgacctgct gctggagatg ctggacgccc accgcctaca tgcgcccact agccgtggag
     2041 gggcatccgt ggaggaaacg gaccaaagcc acttggccac tgcgggctct acttcatcgc
     2101 attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc acagtcgcgg
     2161 ctgcaggtga cggtgctggt ttaattaaca tgacggtcga ccatgatttc aatagcgaag
     2221 atattttatt ccccatagaa agcatgagta gtatacaata cgtggagaat aataacccaa
     2281 ataatattaa caacgatgtt atcccgtatt ctctagatat caaaaacact gtcttagata
     2341 gtgcggatct caatgacatt caaaatcaag aaacttcact gaatttgggg cttcctccac
     2401 tatctttcga ctctccactg cccgtaacgg aaacgatacc atccactacc gataacagct
     2461 tgcatttgaa agctgatagc aacaaaaatc gcgatgcaag aactattgaa aatgatagtg
     2521 aaattaagag tactaataat gctagtggct ctggggcaaa tcaatacaca actcttactt
     2581 caccttatcc tatgaacgac attttgtaca acatgaacaa tccgttacaa tcaccgtcac
     2641 cttcatcggt acctcaaaat ccgactataa atcctcccat aaatacagca agtaacgaaa
     2701 ctaatttatc gcctcaaact tcaaatggta atgaaactct tatatctcct cgagcccaac
     2761 aacatacgtc cattaaagat aatcgtctgt ccttacctaa tggtgctaat tcgaatcttt
     2821 tcattgacac taacccaaac aatttgaacg aaaaactaag aaatcaattg aactcagata
     2881 caaattcata ttctaactcc atttctaatt caaactccaa ttctacgggt aatttaaatt
     2941 ccagttattt taattcactg aacatagact ccatgctaga tgattacgtt tctagtgatc
     3001 tcttattgaa tgatgatgat gatgacacta atttatcacg ccgaagattt agcgacgtta
     3061 taacaaacca atttccgtca atgacaaatt cgaggaatga gctcggatcc taactcgaga
     3121 ttgaattgaa ttgaaatcga tagatcaatt tttttctttt ctctttcccc atcctttacg
     3181 ctaaaataat agtttatttt attttttgaa tattttttat ttatatacgt atatatagac
     3241 tattatttat cttttaatga ttattaagat ttttattaaa aaaaaattcg ctcctctttt
     3301 aatgccttta tgcagttttt ttttcccatt cgatatttct atgtgctgcc aaaccagatg
     3361 tcaacacagc tacaacgccc cattatctta gcctaaaaaa accttctctt tggaactttc
     3421 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg
     3481 cgacagccct ccgacggatg actctcctcc gtgcgtcctc gtcatcaccg gtcgcgttcc
     3541 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     3601 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta
     3661 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3721 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc
     3781 tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa
     3841 tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3901 aaaaactata agatctatgg aggaagacaa catggctatc atcaaggaat tcatgagatt
     3961 caaggtccac atggaaggtt ctgtcaacgg tcacgaattt gaaattgaag gtgaaggtga
     4021 aggtcatcca tacgaaggta ctcaaacagc tagactaaag gttaccaagg gtgacccatt
     4081 gccttttgct tgggatatct tgtctccaca attcatgtac ggttccaagg cttacgttaa
     4141 gcacccagct gacattccag actacttgaa attatctttc ccagaaggtt tcacctggga
     4201 aagagttatg aacttcgaag acggcggagt tgttgctgtc acccaagact cctccttgca
     4261 agatggtcaa ttcatctaca aggtcaagtt gttgggtatt aatttcccat cagatggtcc
     4321 agtcatgcaa aagaagacca tgggttggga agcttccact gaaagaatgt acccagaaga
     4381 tggtgctttg aagggtgaaa tcaaccaaag attgaaatta aaggacggtg gtcactacga
     4441 tgctgaagtc aagaccactt acagagccaa gaagccagtt caattgccag gtgcctacga
     4501 tgttgacatt aaattggaca tcacttctca caacgaagat ggatccggtt cattgggaag
     4561 aggtagaaga tccaaattgt aataactcga gtggcagctt ttgattaagc cttctagtcc
     4621 aaaaaacacg tttttttgtc atttatttca ttttcttaga atagtttagt ttattcattt
     4681 tatagtcacg aatgttttat gattctatat agggttgcaa acaagcattt ttcattttat
     4741 gttaaaacaa tttcaggttt accttttatt ctgcttgtgg tgacgcgtgt atccgcccgc
     4801 tcttttggtc acccatgtat gctggatgca cacactggct taagatgaca acgtcttggg
     4861 gccttaccac cagtggactt tcttgctgtt tgctttgttc tggccattgt ttgcgtttat
     4921 atatttatgt tagatgtttt tcttattaac tagaaagaaa gaatataaaa ggttgaggaa
     4981 agagatgtat cccgaagaat acacagtctt ttatatatgt atttcaacaa ggagccgtgg
     5041 agggtactaa aaagaaaaat cgcccgggca tttcgttatc ttccacgcta aaagtcaagg
     5101 agagatatta cggccaggat cgcaaaggtg cagagcaagg aaatgtgaga aattgtgaga
     5161 acgataatgt atgggacaat gcgaaaatgt gagaacgaga gcaaaaatct tttttgtatc
     5221 tccccgccga atttggaaac cgcgttctga aaacttcgca tcttcacata gtaaaactgt
     5281 tccgagcgct tctccccata atggttagtg gtaaaaaccg aagttgttta ctttagcaaa
     5341 tgcccgcgaa tacggtggta aattgccacc cccccttccc cattcattgg gtaaagacca
     5401 atttgatgga taaattggtt gtggaaaagg tctaattctt tttcctataa ataccgagat
     5461 attttttcta tatgatggtt tccgtcgcat tattgtactc tatagtacta aagcaacaaa
     5521 caaaaacaag caacaaatat aatatagtaa aatagatcta tgccaccacc atcaagaagt
     5581 agaataaaca aaacaagaac attaggaata gtgggtacag ctatagcagt gttggtcacg
     5641 tcctactata tatatcaaaa ggtgacaggt tcttctaaag gtgaagaatt attcactggt
     5701 gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc
     5761 ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaattgat ttgtactact
     5821 ggtaaattgc cagttccatg gccaacctta gtcactactt taggttatgg tttgcaatgt
     5881 tttgctagat acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa
     5941 ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct
     6001 gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt
     6061 aaagaaggtg gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt
     6121 tacatcactg ctgacaaaca aaagaatggt atcaaagcta acttcaaaat tagacacaac
     6181 attgaagatg gtggtgttca attagctgac cattatcaac aaaatactcc aattggtgat
     6241 ggtccagtct tgttaccaga caaccattac ttatcctatc aatctgcctt atccaaagat
     6301 ccaaacgaaa agagagatca catggtcttg ttagaatttg ttactgctgc tggtattacc
     6361 catggtatgg atgaattgta caaaggatcc agtggaagtt caggctccgg ttctgggcct
     6421 cccccagctc ttccgccaaa aagacgaagg tctggcagtt ccggatcagg ttcagggcca
     6481 cctccagcat tacctccaaa gaggaggaga tccggctcat ctggttcagg atccggccct
     6541 ccgcctgcct tgccaccaaa acgtagacgt agcggatcat ccggatctgg gagtggtcct
     6601 ccacctgcac taccacctaa gaggcgtagg tcaggtagtt ccgggtcagg atcaggacca
     6661 ccgcctgctt tgcctccaaa acgtaggcgt taatggcagt gcttttaact aagaattatt
     6721 agtcttttct gcttattttt tcatcatagt ttagaacact ttatattaac gaatagttta
     6781 tgaatctatt taggtttaaa aattgataca gttttataag ttactttttc aaagactcgt
     6841 gctgtctatt gcataatgca ctggaagggg aaaaaaaagg tgcacacgcg tggctttttc
     6901 ttgaatttgc agtttgaaaa atgctgagca gttacagaga tgttacgaac cactagtgca
     6961 ctgcagtaca tcgaggagaa cttctagtat atctacatac ctaatattat tgccttatta
     7021 aaaatggaat cccaacaatt acatcaaaat ccacattctc ttcaaaatca attgtcctgt
     7081 acttccttgt tcatgtgtgt tcaaaaacgt tatatttata ggataattat actctatttc
     7141 tcaacaagta attggttgtt tggccgagcg gtctaaggcg cctgattcaa gaaatatctt
     7201 gaccgcagtt aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta
     7261 gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat
     7321 caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt
     7381 ttcaactgaa aaattgggag aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat
     7441 agaatagaga agcgttcatg actaaatgct tgcatcacaa tacttgaagt tgacaatatt
     7501 atttaaggac ctattgtttt ttccaatagg tggttagcaa tcgtcttact ttctaacttt
     7561 tcttaccttt tacatttcag caatatatat atatatattt caaggatata ccattctaat
     7621 gtctgcccct aagaagatcg tcgttttgcc aggtgaccac gttggtcaag aaatcacagc
     7681 cgaagccatt aaggttctta aagctatttc tgatgttcgt tccaatgtca agttcgattt
     7741 cgaaaatcat ttaattggtg gtgctgctat cgatgctaca ggtgttccac ttccagatga
     7801 ggcgctggaa gcctccaaga aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa
     7861 atggggaacc ggtagtgtta gacctgaaca aggtttacta aaaatccgta aagaacttca
     7921 attgtacgcc aacttaagac catgtaactt tgcatccgac tctcttttag acttatctcc
     7981 aatcaagcca caatttgcta aaggtactga cttcgttgtt gtcagagaat tagtgggagg
     8041 tatttacttt ggtaagagaa aggaagacga tggtgatggt gtcgcttggg atagtgaaca
     8101 atacaccgtt ccagaagtgc aaagaatcac aagaatggcc gctttcatgg ccctacaaca
     8161 tgagccacca ttgcctattt ggtccttgga taaagctaat gttttggcct cttcaagatt
     8221 atggagaaaa actgtggagg aaaccatcaa gaacgaattt cctacattga aggttcaaca
     8281 tcaattgatt gattctgccg ccatgatcct agttaagaac ccaacccacc taaatggtat
     8341 tataatcacc agcaacatgt ttggtgatat catctccgat gaagcctccg ttatcccagg
     8401 ttccttgggt ttgttgccat ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt
     8461 tggtttgtac gaaccatgcc acggttctgc tccagatttg ccaaagaata aggtcaaccc
     8521 tatcgccact atcttgtctg ctgcaatgat gttgaaattg tcattgaact tgcctgaaga
     8581 aggtaaggcc attgaagatg cagttaaaaa ggttttggat gcaggtatca gaactggtga
     8641 tttaggtggt tccaacagta ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa
     8701 aatccttgct taaaaagatt ctcttttttt atgatatttg tacataaact ttataaatga
     8761 aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa
     8821 ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata
     8881 caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac
     8941 attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag aaaatcatga
     9001 aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa aatacaacaa
     9061 ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc ttcttgaacc
     9121 atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac ggccttaacg
     9181 acgtagtcga gagtactcgt atcgcatgtc ggtgcgacac gaaattacaa aatggaatat
     9241 gttcataggg tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa
     9301 aggaggatgt aaaggaatac aggtaagcaa attgatacta atggctcaac gtgataagga
     9361 aaaagaattg cactttaaca ttaatattga caaggaggag ggcaccacac aaaaagttag
     9421 gtgtaacaga aaatcatgaa actatgattc ctaatttata tattggagga ttttctctaa
     9481 aaaaaaaaaa atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa
     9541 gtcaatacct tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct
     9601 gtcagaaacg gccttaacga cgtagtcgac ctcctcttca gtactaaatc taccaatacc
     9661 aaatctgatg gaagaatggg ctaatgcatc atccttaccc agcgccgagc ggccgcgtgt
     9721 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     9781 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     9841 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     9901 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     9961 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
    10021 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
    10081 aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa
    10141 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
    10201 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
    10261 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
    10321 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
    10381 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
    10441 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
    10501 tccatgttgg aatttaatcg cggcctggag caagacgttt cccgttgaat atggctcata
    10561 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
    10621 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
    10681 cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat cccttaacgt gagttttcgt
    10741 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10801 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10861 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10921 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10981 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11041 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    11101 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    11161 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    11221 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    11281 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    11341 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    11401 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    11461 tggataaccg tgcggccgcc aatacccaga agaacagtaa aataaagcaa ggtacgtgaa
    11521 attaatattt ttaaatggtt ctaaccgatg ccgaagaact gcgcagtccg gttataacgt
    11581 ctgacatgtc cttttttgat ttggaatcca accactcaag tgactctgtt catttacttt
    11641 gcgaaaaata tacccacaaa ttgcccatcg aaagtgaatc gcaaaccacc ttcagactgg
    11701 caccgacaaa gcaaagatta tacagacaga gtactttata cgtaccgtta agtctcaagc
    11761 aaagggtttt cttatttact gaacgggtaa agagtatctg ggccggcttg ccaagatgca
    11821 aaccgaataa gtatttcaaa gttgcatttg ccttagccgt cctgacacca ttggctattt
    11881 ggatatttta tattgacttt cgtgtacatt gatcacatcg actgttctat tggcaaatga
    11941 accacgggca ttgactattt ttcaggttac tactatatat tatcatcacg ggcaaggatt
    12001 gtaccctgaa ttcgcatcta gactga
//
