LOCUS       sequence-47591-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Miyamoto T, Derose R, Suarez A, Ueno T, Chen M, Sun TP, Wolfgang MJ,
            Mukherjee C, Meyers DJ, Inoue T
  TITLE     Rapid and orthogonal logic gating with a gibberellin-induced 
            dimerization system.
  JOURNAL   Nat Chem Biol. 2012 Mar 25;8(5):465-70. doi: 10.1038/nchembio.922.
  PUBMED    22446836
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2012-03-25- 25"; volume: "8"; 
            issue: "5"; pages: "465-70"
COMMENT     Sequence Label: NLS-GAI(1-92)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    126..191
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(447..464)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(498..518)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(522..542)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(546..566)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(607..810)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(636..656)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat
      121 ccggtggatc ccgggcccgc ggtaccgtcg actgcagaat tcgaagcttg agctcgagat
      181 ctgagtccgg acttgtacag attaaggtcg gtgagcatag aatcaagcca cgtgtaaagc
      241 tccgccggat tatagtgaac agtctcagta gcgagttgag aaagatcgtc ttcttgaaca
      301 ttagacatca taacttcaag ctgctcgagt ttctgagcaa catcagccat ttcggatgac
      361 ctaaccttgt aaccaagaac agctagaagc tcatccatgc cgttaccgtc gtcttcttca
      421 ttcatcatca tagtcttctt atcttgatga tgatgatgat gatgatctct cttcatggtg
      481 gcgaccggtg actgcagtac ctttctcttc ttttttggat ctacctttct cttctttttt
      541 ggatctacct ttctcttctt ttttggatcc atggtggcgc tagcggatct gacggttcac
      601 taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa
      661 tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact
      721 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
      781 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      841 gtactgccaa gta
//
