LOCUS       sequence-475912-       11792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, 
            and inactive Pex22 peroxisomal chauffeur as negative control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11792)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1577
FEATURES             Location/Qualifiers
     source          1..11792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5762..5949
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5817..5841)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        6293..6706
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6840..7547
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             8065..8868
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9510..10325)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9510..10325)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10233..10252
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10317..10336
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10550..11138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11039..11058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatgagtatt tcaaattgga tcaccactgc gtatttaatt acatcaacat
      781 cttttcaacc tctttatggg tcattttctg atgcacttgg tcgaagaaac tgccttttct
      841 ttgctaatgg ggcttttacc attggatgtc tagcctgtgg tttctcgaaa aacatctaca
      901 tgcttagttt tatgagagca ttgacaggca taggaggtgg tggcttgatc acactttcta
      961 caatcgtaaa ttcagacgtt attccaagtt cgaaaagagg aatttttcaa gcgtttcaga
     1021 atttactttt gggatttggt gccatatgtg gagcgtcttt cggtggcaca atagcgtcga
     1081 gcattggttg gaggtggtgt tttctcatcc aagtacccat atctgtgatt agttccatat
     1141 taatgaatta ttatgtacct aatcagaaag aatataatcg tcaaaattct agcatattcc
     1201 aaaatcccgg aaaaatactc agggacatag atgttatggg ctcaattctt attataactg
     1261 gactcacact acagcttctt tacctgagcc tggggtgttc tacttctaaa ttatcatgga
     1321 ccagcccttc tgtgctactg ctattagttg ggagtgtaat aatcctctta ctgttcatat
     1381 tgcacgaaag gaaaacaagt gctagagcga ttattcctat ggagctggtc aattcctcct
     1441 acagtgtcgt tgtactttcg ataagtatac ttgttggttt tgccagctac gcgtatcttt
     1501 ttactttacc attattcttt cagattgtac ttggagattc cactgcaaaa gcaggattac
     1561 gtcttacgat tccttcccta tttactccgg taggcagtct cataacagga ttttccatga
     1621 gcaagtacaa ctgtctaaga ttattactct acattggtat ttctttgatg tttttgggta
     1681 actttttatt cctgtttatt gaaaaaactt ctccgaactg gttgattggt ctatttttga
     1741 tacctgcaaa tctaggacaa ggtatcactt ttcctacgac cttgtttact ttcatattta
     1801 tgttctctaa gagtgaccaa gctactgcga catcaacttt atatttattc cgtagtattg
     1861 gatctgtatg gggtgttgca atttcagctg gcgtcattca attatctttc gcaggtttat
     1921 tgcgtagtaa tttgaaaggt ctactggatg aaaacaagat aaagaaactt attgttcagc
     1981 ttagtgcaaa ctcctcatat attggatctt tacatggcga agttaaaaac acagtcataa
     2041 agagttttga tgaggcaaca aagagggctc atctaatgtc tacattactc tcttcattgg
     2101 ccctgatact ctgcatcctt aaagacaatc tggcgaaacc taaaacaaga agaggttctg
     2161 tttctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg
     2221 atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta
     2281 aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag
     2341 tcactacttt atcttggggt gttcaatgtt ttgcaagata cccagatcat atgaaacaac
     2401 atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca
     2461 aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta
     2521 atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat
     2581 tggaatacaa ttatttctct gacaatgttt acatcactgc tgacaaacaa aagaatggta
     2641 tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg tggtgttcaa ttagctgacc
     2701 attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact
     2761 tatccactca atctaagtta tccaaagatc caaacgaaaa gagggaccac atggtcttgt
     2821 tagaatttgt tactgctgct ggtattacct tgggtatgga tgaattgtac aaaggatccg
     2881 cagagtatgt gcgggccctg tttgacttta atgggaatga tgaagaagac cttcccttta
     2941 agaaaggaga catcctgaga atccgggata agcctgaaga gcagtggtgg aatgcagagg
     3001 acagcgaagg aaagaggggg atgattcctg tcccttacgt ggagaagtat ggatctggga
     3061 gcggatctgg aggcagcatg gcggaatatg tacgagctct ttttgatttt aatggcaatg
     3121 atgaggagga tctccctttt aaaaagggcg atattctgcg cattcgtgat aagccagagg
     3181 aacaatggtg gaacgccgag gatagtgagg ggaaacgcgg catgattccg gtgccgtatg
     3241 tagagaagta ctaatggcgc caattggtgc ggcaattgat aataacgaaa atgtctttta
     3301 atgatctggg tataatgagg aattttccga acgtttttac tttatatata tatatacatg
     3361 taacatatat tctatacgct atagagaaag gaaatttttc aattaaaaaa aaaatagaga
     3421 aagagtttca cttcttgatt atcgctaaca ctaatggttg aagtactgct actttaattt
     3481 tatgctgcca aaccagatgt caacacagct acaacgtata tattaaattt gctcttgttc
     3541 tgtactttcc taattcttat gtaaaaagac aagaatttat gatactattt aataacaaaa
     3601 aactacctaa gaaaagcatc atgcagtcga aattgaaatc gaaaagtaaa actttaacgg
     3661 aacatgtttg aaattctaag aaagcataca tcttcatccc ttatatatag agttatgttt
     3721 gatattagta gtcatgttgt aatctctggc ctaagtatac gtaacgaaaa tggtagcacg
     3781 tcgcgtttat ggcccccagg ttaatgtgtt ctctgaaatt cgcatcactt tgagaaataa
     3841 tgggaacacc ttacgcgtga gctgtgccca ccgcttcgcc taataaagcg gtgttctcaa
     3901 aatttctccc cgttttcagg atcacgagcg ccatctagtt ctggtaaaat cgcgcttaca
     3961 agaacaaaga aaagaaacat cgcgtaatgc aacagtgaga cacttgccgt catatataag
     4021 gttttggatc agtaaccgtt atttgagcat aacacaggtt tttaaatata ttattatata
     4081 tcatggtata tgtgtaaaat ttttttgctg actggttttg tttatttatt tagcttttta
     4141 aaaattttac tttcttcttg ttaatttttt ctgattgctc tatactcaaa ccaacaacaa
     4201 cttactctac aactaagatc tatgctcata gaaaacacta atgatcggtt tggtatcgtc
     4261 atagatgcag ggtcttcggg ttcgagaatc catgtgttta agtggcagga cacggaatcg
     4321 cttcttcatg caacaaacca ggactcacag tccatattac aatcagtacc tcatattcat
     4381 caagaaaaag actggacttt taagctgaat ccaggcttgt cgagttttga aaaaaaacct
     4441 caagatgcgt acaaatctca catcaagccg ttactagatt ttgctaagaa tattatccct
     4501 gaatcacatt ggtcgagttg tcccgttttt attcaagcaa ccgcgggcat gcgcctttta
     4561 cctcaagaca tacaatcttc cattttggat ggtttgtgtc aggggctcaa acaccctgca
     4621 gaatttttgg ttgaggattg ctcagcacaa attcaagtca ttgatggtga aaccgaaggt
     4681 ttatatggct ggcttggctt aaactatcta tatggacact ttaatgacta taatccagaa
     4741 gtctctgacc attttacatt tgggtttatg gacatgggcg gtgcctctac tcagattgcg
     4801 tttgcaccgc atgattcagg agaaatagct agacatagag atgacatcgc caccatcttc
     4861 ttgaggagtg ttaacggaga tttgcagaaa tgggacgttt ttgttagtac gtggttaggg
     4921 tttggtgcca atcaggctag aagaaggtac ttggctcagt tgatcaatac ccttccagaa
     4981 aacacaaatg attacgaaaa tgatgacttc tctacaagga acttgaatga tccatgtatg
     5041 ccgcgaggaa gcagcacgga ttttgaattt aaagatacca tatttcacat cgcgggttct
     5101 gggaattacg aacaatgtac aaaatctatt tatccgttac ttttaaagaa catgccttgt
     5161 gatgacgagc cgtgcttgtt taatggtgtt catgctcctc gaatagactt tgccaatgac
     5221 aaatttatag gtacttctga gtactggtac actgccaacg acgtattcaa acttgggggc
     5281 gaatacaact ttgacaaatt tagcaaaagc ctaagggagt tttgcaattc caattggacg
     5341 caaatattag cgaacagtga taaaggcgtg tataattcta ttccggagaa ttttttgaaa
     5401 gatgcatgct ttaagggcaa ttgggtcctt aatatacttc atgaagggtt tgatatgcct
     5461 cgaatagatg tcgacgcaga aaatgtcaat gacaggccct tatttcaaag tgtggaaaaa
     5521 gtcgaagaac gagagttatc gtggacacta ggcagaattt tactttatgc ctcaggtagc
     5581 atattggcag gtaatgatga cttcatggtg ggtattgcgc ccagtgaaag aagaactaag
     5641 ctcactggta agaaattcat acctggcaag ttactggagt ctgatcaact acgcaagcaa
     5701 agttccggat ccggttcatt gggaagaggt agaagatcca aattgtaata actcgagtgg
     5761 cgcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     5821 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     5881 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     5941 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg gatgcacaca
     6001 ctggcttaag atgacaacgc cttgccaaca gggagttctt cagagacatg gaggctcaaa
     6061 acgaaattat tgacagccta gacatcaata gtcatacaac agaaagcgac cacccaactt
     6121 tggctgataa tagcgtataa acaatgcata ctttgtacgt tcaaaataca atgcagtaga
     6181 tatatttatg catattacat ataatacata tcacatagga agcaacaggc gcgttggact
     6241 tttaattttc gaggaccgcg aatccttaca tcacacccaa tcccccacaa gtgatccccc
     6301 acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact
     6361 ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt tcccctcttt
     6421 cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagacaccg
     6481 cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct
     6541 tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat atttaagtta
     6601 ataaacggtc atcaatttct caagtttcag tttcattttt cttgttctat tacaactttt
     6661 tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt aattacaaaa
     6721 gatctatgcc accaccatca agaagtagaa taaacaaaac aagaacatta ggaatagtgg
     6781 gtacagctat agcagtgttg gtcacgtcct actatatata tcaaaaggtg acaggttctg
     6841 tgtccaaagg agaggagtta atcaaggaaa acatgagaat gaaagttgtc atggagggct
     6901 ccgttaatgg tcaccaattc aagtgtacag gggaaggtga aggtaatcct tacatgggta
     6961 cacaaactat gagaattaaa gtaattgaag gcggaccact accatttgca tttgacattc
     7021 tggcaacgtc attcatgtac ggatcacgaa ctttcatcaa gtaccctaaa ggtataccag
     7081 actttttcaa gcaatctttt ccagagggtt ttacatggga aagggttaca agatacgaag
     7141 atgggggtgt cgtcacagtt atgcaagata cttcattaga agatggctgc cttgtctatc
     7201 atgtgcaagt aagaggggtg aattttcctt ctaacggacc tgtgatgcag aaaaagacca
     7261 aaggttggga accaaatact gaaatgatgt acccagctga tggaggtttg agaggctaca
     7321 cacacatggc gcttaaagtt gatggtggag gtcatttgtc ttgtagtttt gttaccactt
     7381 atcgttctaa aaagactgtt ggcaatatca aaatgccagg aatacatgct gtagaccaca
     7441 gactagaaag actcgaagag agcgataacg aaatgttcgt tgtacagaga gagcatgccg
     7501 tagccaaatt tgctggctta ggcggtggta tggatgaatt gtataaggga tccgggtcag
     7561 gctccggatc tggccctccc gcagctcttg cgccaaaaag acgaaggtaa tggcagtgct
     7621 tttaactaag aattattagt cttttctgct tattttttca tcatagttta gaacacttta
     7681 tattaacgaa tagtttatga atctatttag gtttaaaaat tgatacagtt ttataagtta
     7741 ctttttcaaa gactcgtgct gtctattgca taatgcactg gaaggggaaa aaaaaggtgc
     7801 acacgcgtgg ctttttcttg aatttgcagt ttgaaaaatg ctgagcagtt acagagatgt
     7861 tacgaaccac tagtgcactg cagtacacgg tttccttgaa atttttttga ttcggtaatc
     7921 tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata
     7981 tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa
     8041 acgtgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact
     8101 catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca aacaaacttg
     8161 tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga agcattaggt
     8221 cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc catggagggc
     8281 acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt cgaagacaga
     8341 aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt atacagaata
     8401 gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt
     8461 ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat gttagcagaa
     8521 ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt tgacattgcg
     8581 aagagcgaca aagattttgt tatcggcttt attgctcaaa gagacatggg tggaagagat
     8641 gaaggttacg attggttgat tatgacaccc ggtgtgggtt tagatgacaa gggagatgca
     8701 ttgggtcaac agtatagaac cgtggatgat gtggtttcta caggatctga cattattatt
     8761 gttggaagag gactatttgc aaagggaagg gatgctaagg tagagggtga acgttacaga
     8821 aaagcaggct gggaagcata tttgagaaga tgcggccagc aaaactaaaa aactgtatta
     8881 taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt atatcagtta
     8941 ttacccgagt agagcacttg aatccactgc cccgggaatc tcggtcgtaa tgatttctat
     9001 aatgacgaaa aaaaaaaaat tggaaagaaa aagcttcatg gcctttataa aaaggaacta
     9061 tccaatacct cgccagaacc aagtaacagt attttacggg gcacaaatca agaacaataa
     9121 gacaggactg taaagatgga cgcattgaac tccaaagaac aacaagagtt ccaaaaagta
     9181 gtggaacaaa agcaaatgaa ggatttcatg cgtttgtact ctaatctggt agaaagatgt
     9241 ttcacagact gtgtcaatga cttcacaaca tcaaagctaa ccaataagga acaaacatgc
     9301 atcatgaagt gctcagaaaa gttcttgaag catagcgaac gtgtagggca gcgtttccaa
     9361 gaacaaaacg ctgccttggg acaaggcttg ggccgataag gtgtactggc gtatatatat
     9421 ctaattatgt atctctggtg tagcccattt ttagcatgta aatataaaga ccgagcggcc
     9481 gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     9541 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     9601 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     9661 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     9721 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     9781 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     9841 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac gcgatcgctg
     9901 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     9961 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
    10021 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
    10081 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
    10141 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
    10201 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
    10261 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
    10321 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
    10381 atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga
    10441 ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct taacgtgagt
    10501 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10561 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10621 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10681 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10741 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10801 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10861 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10921 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10981 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11041 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11101 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11161 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11221 attctgtgga taaccgtgcg gccgccaatg ctaaattcga gtgaaacaca ggaagatcag
    11281 aaaatcctca tttcatccat attaacaata atttcaaatg tttatttgca ttatttgaaa
    11341 ctaggcaaga caagcaacga aacgtttttg aaaattttga gtattttcaa taaatttgta
    11401 gaggactcag atattgaaaa aaagctacag caattaatac ttgataagaa gagtattgag
    11461 aagggcaacg gttcatcatc tcatggatct gcacatgaac aaacaccaga gtcaaacgac
    11521 gttgaaattg aggctactgc gccaattgat gacaatacag acgatgataa caaaccgaag
    11581 ttatctgatg tagaaaagga ttaaagatgc taagagatag tgatgatatt tcataaataa
    11641 tgtaattcta tatatgttaa ttaccttttt tgcgaggcat atttatggtg aaggataagt
    11701 tttgaccatc aaagaaggtt aatgtggctg tggtttcagg gtccataaag cccacatgga
    11761 taacattacc cctgaattcg catctagact ga
//
