LOCUS       sequence-475932-        8569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables expression of Ovalbumin with Blasticidin selectable marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   LaFleur MW, Milling LE, Prathima P, Li V, Lemmen AM, Streeter ISL, 
            Heisig PKS, Derosia NM, Riffo E, Xu H, Nguyen TH, Kolengaden A, 
            Markson SC, Doench JG, Sharpe AH
  TITLE     A STUB1-CHIC2 complex inhibits CD8(+) T cells to restrain tumor 
            immunity.
  JOURNAL   Nat Immunol. 2025 Sep;26(9):1476-1487. doi: 
            10.1038/s41590-025-02231-6. Epub 2025 Aug 12.
  PUBMED    40796662
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-025-02231-6"; journalName: "Nat Immunol"; date: 
            "2025-09"; volume: "26"; issue: "9"; pages: "1476-1487"
COMMENT     Sequence Label: pLX_305_OVA_Blasticidin
FEATURES             Location/Qualifiers
     source          1..8569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     411..430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     664..681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    798..819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(834..839,840..857,858..864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    872..888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     877..899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     896..912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     896..912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     931..951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        933..951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        979..1205
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1206..1386
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1433..1558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2051..2284
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2469..2513
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2744..2954
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2766..2962
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2813..2948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2984..3379
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3453..3570
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3628..4128
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4047..4065
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4149..5309
                     /codon_start=1
                     /product="chicken ovalbumin"
                     /label=ovalbumin
                     /translation="MGSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLG
                     AKDSTRTQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFSLAS
                     RLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVESQTNGIIRNVLQ
                     PSSVDSQTAMVLVNAIVFKGLWEKTFKDEDTQAMPFRVTEQESKPVQMMYQIGLFRVAS
                     MASEKMKILELPFASGTMSMLVLLPDEVSGLEQLESIINFEKLTEWTSSNVMEERKIKV
                     YLPRMKMEEKYNLTSVLMAMGITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGR
                     EVVGSAEAGVDAASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP"
     misc_feature    5346..5934
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5399..5419)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5817..5828)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6061..6094)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6114..6294
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    6371..6492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6408..6427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6462..6481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6532..6667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6687..6706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6688..6706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6716..6733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6716..6732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6725..6747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6874..7329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6961..6980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7171..7192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7355..7459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7460..7528,7529..8320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7678..7697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8491..8569,1..510)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
       61 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      121 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      181 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      241 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      301 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      421 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
      481 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
      541 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
      601 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      661 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      721 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
      781 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      841 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      901 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
      961 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     1021 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     1081 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     1141 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     1201 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     1261 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     1321 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     1381 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     1441 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     1501 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     1561 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1621 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1681 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1741 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     1801 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     1861 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     1921 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     1981 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     2041 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     2101 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     2161 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     2221 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     2281 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     2341 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     2401 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     2461 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     2521 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     2581 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     2641 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct
     2701 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2761 aagcatgcgt ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2821 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2881 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2941 tggaggccta ggcttttgca aaaagctttc tagaggtacc accatggcca agcctttgtc
     3001 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     3061 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     3121 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     3181 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     3241 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     3301 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     3361 tggttatgtg tgggagggcc tgcagctgca gtagtaaggc gcgccgttaa cgaattctag
     3421 atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3481 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3541 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3601 ctttggcgcc ggctcgaggc ctgcaggttg cgccttttcc aaggcagccc tgggtttgcg
     3661 cagggacgcg gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct
     3721 cgcacattct tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc
     3781 cccccggcga cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg
     3841 tgccggacgt gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc
     3901 cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca
     3961 gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta
     4021 gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg
     4081 tcggcagtcg gctccctcgt tgaccgaatc accgacctct ctccccaggg gctagcatcg
     4141 attgatcaat gggctccatc ggcgcagcaa gcatggaatt ttgttttgat gtattcaagg
     4201 agctcaaagt ccaccatgcc aatgagaaca tcttctactg ccccattgcc atcatgtcag
     4261 ctctagccat ggtatacctg ggtgcaaaag acagcaccag gacacagata aataaggttg
     4321 ttcgctttga taaacttcca ggattcggag acagtattga agctcagtgt ggcacatctg
     4381 taaacgttca ctcttcactt agagacatcc tcaaccaaat caccaaacca aatgatgttt
     4441 attcgttcag ccttgccagt agactttatg ctgaagagag atacccaatc ctgccagaat
     4501 acttgcagtg tgtgaaggaa ctgtatagag gaggcttgga acctatcaac tttcaaacag
     4561 ctgcagatca agccagagag ctcatcaatt cctgggtaga aagtcagaca aatggaatta
     4621 tcagaaatgt ccttcagcca agctccgtgg attctcaaac tgcaatggtt ctggttaatg
     4681 ccattgtctt caaaggactg tgggagaaaa catttaagga tgaagacaca caagcaatgc
     4741 ctttcagagt gactgagcaa gaaagcaaac ctgtgcagat gatgtaccag attggtttat
     4801 ttagagtggc atcaatggct tctgagaaaa tgaagatcct ggagcttcca tttgccagtg
     4861 ggacaatgag catgttggtg ctgttgcctg atgaagtctc aggccttgag cagcttgaga
     4921 gtataatcaa ctttgaaaaa ctgactgaat ggaccagttc taatgttatg gaagagagga
     4981 agatcaaagt gtacttacct cgcatgaaga tggaggaaaa atacaacctc acatctgtct
     5041 taatggctat gggcattact gacgtgttta gctcttcagc caatctgtct ggcatctcct
     5101 cagcagagag cctgaagata tctcaagctg tccatgcagc acatgcagaa atcaatgaag
     5161 caggcagaga ggtggtaggg tcagcagagg ctggagtgga tgctgcaagc gtctctgaag
     5221 aatttagggc tgaccatcca ttcctcttct gtatcaagca catcgcaacc aacgccgttc
     5281 tcttctttgg cagatgtgtt tccccttaat accggttaag tcgacaatca acgcgttaag
     5341 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5401 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5461 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     5521 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     5581 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     5641 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     5701 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     5761 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5821 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5881 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     5941 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6001 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga
     6061 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc
     6121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcatacgta
     6301 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6361 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6481 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     6541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6601 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     6661 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     6721 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     6781 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     6841 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     6901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7021 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7081 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7201 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7321 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     7741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     7801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     7861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     7921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     7981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccg
//
