LOCUS       sequence-475985-        7619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter
            (pTet) on a low-copy replication origin (SC101) as part of the ADEPT
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Son HI, Hamrick GS, Shende AR, Kim K, Yang K, Huang TJ, You L
  TITLE     Population-level amplification of gene regulation by programmable 
            gene transfer.
  JOURNAL   Nat Chem Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9.
  PUBMED    39779901
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Jan 8. doi: 10.1038/s41589-024-01817-9."
COMMENT     Sequence Label: ADEPT-pCas9
FEATURES             Location/Qualifiers
     source          1..7619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..223
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             271..1221
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(1379..1406)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1422..1493)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1520..1552)
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag
                     /note="wild-type LAA variant"
                     /translation="AANDENYALAA"
     CDS             complement(1553..5656)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(1553..5656)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             5663..5674
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        5692..5747
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    5693..5711
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             5763..6389
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(5839..5858)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        6482..6584
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             6585..7244
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6651..6670)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      7271..7365
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gagttataca cagggctggg atctattctt tttatctttt tttattcttt ctttattcta
       61 taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc ggaatttaca
      121 gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt acagataccc
      181 acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa ttggtatagt
      241 gattaaaatc acctagacca attgagatgt atgtctgaat tagttgtttt caaagcaaat
      301 gaactagcga ttagtcgcta tgacttaacg gagcatgaaa ccaagctaat tttatgctgt
      361 gtggcactac tcaaccccac gattgaaaac cctacaagga aagaacggac ggtatcgttc
      421 acttataacc aatacgctca gatgatgaac atcagtaggg aaaatgctta tggtgtatta
      481 gctaaagcaa ccagagagct gatgacgaga actgtggaaa tcaggaatcc tttggttaaa
      541 ggctttgaga ttttccagtg gacaaactat gccaagttct caagcgaaaa attagaatta
      601 gtttttagtg aagagatatt gccttatctt ttccagttaa aaaaattcat aaaatataat
      661 ctggaacatg ttaagtcttt tgaaaacaaa tactctatga ggatttatga gtggttatta
      721 aaagaactaa cacaaaagaa aactcacaag gcaaatatag agattagcct tgatgaattt
      781 aagttcatgt taatgcttga aaataactac catgagttta aaaggcttaa ccaatgggtt
      841 ttgaaaccaa taagtaaaga tttaaacact tacagcaata tgaaattggt ggttgataag
      901 cgaggccgcc cgactgatac gttgattttc caagttgaac tagatagaca aatggatctc
      961 gtaaccgaac ttgagaacaa ccagataaaa atgaatggtg acaaaatacc aacaaccatt
     1021 acatcagatt cctacctaca taacggacta agaaaaacac tacacgatgc tttaactgca
     1081 aaaattcagc tcaccagttt tgaggcaaaa tttttgagtg acatgcaaag taagtatgat
     1141 ctcaatggtt cgttctcatg gctcacgcaa aaacaacgaa ccacactaga gaacatactg
     1201 gctaaatacg gaaggatctg aggttcttat ggctcttgta tctatcagtg aagcatcaag
     1261 actaaccaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1321 tttgagtgag ctgataccgc tcgccgcagc cgaacgccga cgtctcccta ggtataaacg
     1381 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa
     1441 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg
     1501 gagatcctta ctcgagttaa gcagccagag cgtagttttc gtcgttagca gcgtcacctc
     1561 ctagctgact caaatcaatg cgtgtttcat aaagaccagt gatggattga tggataagag
     1621 tggcatctaa aacttctttt gtagacgtat atcgtttacg atcaattgtt gtatcaaaat
     1681 atttaaaagc agcgggagct ccaagattcg tcaacgtaaa taaatgaata atattttctg
     1741 cttgttcacg tattggtttg tctctatgtt tgttatatgc actaagaact ttatctaaat
     1801 tggcatctgc taaaataaca cgcttagaaa attcactgat ttgctcaata atctcatcta
     1861 aataatgctt atgctgctcc acaaacaatt gtttttgttc gttatcttct ggactaccct
     1921 tcaacttttc ataatgacta gctaaatata aaaaattcac atatttgctt ggcagagcca
     1981 gctcatttcc tttttgtaat tctccggcac tagccagcat ccgtttacga ccgttttcta
     2041 actcaaaaag actatattta ggtagtttaa tgattaagtc ttttttaact tccttatatc
     2101 ctttagcttc taaaaagtca atcggatttt tttcaaagga acttctttcc ataattgtga
     2161 tccctagtaa ctctttaacg gattttaact tcttcgattt ccctttttcc accttagcaa
     2221 ccactaggac tgaataagct accgttggac tatcaaaacc accatatttt tttggatccc
     2281 agtctttttt acgagcaata agcttgtccg aatttctttt tggtaaaatt gactccttgg
     2341 agaatccgcc tgtctgtact tctgttttct tgacaatatt gacttggggc atggacaata
     2401 ctttgcgcac tgtggcaaaa tctcgccctt tatcccagac aatttctcca gtttccccat
     2461 tagtttcgat tagagggcgt ttgcgaatct ctccatttgc aagtgtaatt tctgttttga
     2521 agaagttcat gatattagag taaaagaaat attttgcggt tgctttgcct atttcttgct
     2581 cagacttagc aatcatttta cgaacatcat aaactttata atcaccatag acaaactccg
     2641 attcaagttt tggatatttc ttaatcaaag cagttccaac gacggcattt agatacgcat
     2701 catgggcatg atggtaattg ttaatctcac gtactttata gaattggaaa tcttttcgga
     2761 agtcagaaac taatttagat tttaaggtaa tcactttaac ctctcgaata agtttatcat
     2821 tttcatcgta tttagtattc atgcgactat ccaaaatttg tgccacatgc ttagtgattt
     2881 ggcgagtttc aaccaattgg cgtttgataa aaccagcttt atcaagttca ctcaaacctc
     2941 cacgttcagc tttcgttaaa ttatcaaact tacgttgagt gattaacttg gcgtttagaa
     3001 gttgtctcca atagtttttc atctttttga ctacttcttc acttggaacg ttatccgatt
     3061 taccacgatt tttatcagaa cgcgttaaga ccttattgtc tattgaatcg tctttaagga
     3121 aactttgtgg aacaatgtga tcgacatcat aatcacttaa acgattaata tctaattctt
     3181 ggtccacata catgtctctt ccattttgga gataatagag atagagcttt tcattttgca
     3241 attgagtatt ttcaacagga tgctctttaa gaatctgact tcctaattct ttgatacctt
     3301 cttcgattcg tttcatacgc tctcgcgaat ttttctggcc cttttgagtt gtctgatttt
     3361 cacgtgccat ttcaataacg atattttctg gcttatgccg ccccattact ttgaccaatt
     3421 catcaacaac ttttacagtc tgtaaaatac cttttttaat agcagggcta ccagctaaat
     3481 ttgcaatatg ttcatgtaaa ctatcgcctt gtccagacac ttgtgctttt tgaatgtctt
     3541 ctttaaatgt caaactatca tcatggatca gctgcataaa attgcgattg gcaaaaccat
     3601 ctgatttcaa aaaatctaat attgttttgc cagattgctt atccctaata ccattaatca
     3661 attttcgaga caaacgtccc caaccagtat aacggcgacg tttaagctgt ttcatcacct
     3721 tatcatcaaa gaggtgagca tatgttttaa gtctttcctc aatcatctcc ctatcttcaa
     3781 ataaggtcaa tgttaaaaca atatcctcta agatatcttc attttcttca ttatccaaaa
     3841 aatctttatc tttaataatt tttagcaaat catggtaggt acctaatgaa gcattaaatc
     3901 tatcttcaac tcctgaaatt tcaacactat caaaacattc tatttttttg aaataatctt
     3961 cttttaattg cttaacggtt acttttcgat ttgttttgaa gagtaaatca acaatggctt
     4021 tcttctgttc acctgaaaga aatgctggtt ttcgcattcc ttcagtaaca tatttgacct
     4081 ttgtcaattc gttataaacc gtaaaatact cataaagcaa actatgtttt ggtagtactt
     4141 tttcatttgg aagattttta tcaaagtttg tcatgcgttc aataaatgat tgagctgaag
     4201 cacctttatc gacaacttct tcaaaattcc atggggtaat tgtttcttca gacttccgag
     4261 tcatccatgc aaaacgacta ttgccacgcg ccaatggacc aacataataa ggaattcgaa
     4321 aagtcaagat tttttcaatc ttctcacgat tgtcttttaa aaatggataa aagtcttctt
     4381 gtcttctcaa aatagcatgc agctcaccca agtgaatttg atggggaata gagccgttgt
     4441 caaaggtccg ttgcttgcgc agcaaatctt cacgatttag tttcaccaat aattcctcag
     4501 taccatccat tttttctaaa attggtttga taaatttata aaattcttct tggctagctc
     4561 ccccatcaat ataacctgca tatccgtttt ttgattgatc aaaaaagatt tctttatact
     4621 tttctggaag ttgttgtcga actaaagctt ttaaaagagt caagtcttga tgatgttcat
     4681 cgtagcgttt aatcattgaa gctgataggg gagccttagt tatttcagta tttactctta
     4741 ggatatctga aagtaaaata gcatctgata aattcttagc tgccaaaaac aaatcagcat
     4801 attgatctcc aatttgcgcc aataaattat ctaaatcatc atcgtaagta tcttttgaaa
     4861 gctgtaattt agcatcttct gccaaatcaa aatttgattt aaaattaggg gtcaaaccca
     4921 atgacaaagc aatgagattc ccaaataagc catttttctt ctcaccgggg agctgagcaa
     4981 tgagattttc taatcgtctt gatttactca atcgtgcaga aagaatcgct ttagcatcta
     5041 ctccacttgc gttaataggg ttttcttcaa ataattgatt gtaggtttgt accaactgga
     5101 taaatagttt gtccacatca ctattatcag gatttaaatc tccctcaatc aaaaaatgac
     5161 cacgaaactt aatcatatgc gctaaggcca aatagattaa gcgcaaatcc gctttatcag
     5221 tagaatctac caattttttt cgcagatgat agatagttgg atatttctca tgataagcaa
     5281 cttcatctac tatatttcca aaaataggat gacgttcatg cttcttgtct tcttccacca
     5341 aaaaagactc ttcaagtcga tgaaagaaac tatcatctac tttcgccatc tcatttgaaa
     5401 aaatctcctg tagataacaa atacgattct tccgacgtgt ataccttcta cgagctgtcc
     5461 gtttgagacg agtcgcttcc gctgtctctc cactgtcaaa taaaagagcc cctataagat
     5521 tttttttgat actgtggcgg tctgtatttc ccagaacctt gaacttttta gacggaacct
     5581 tatattcatc agtgatcacc gcccatccga cgctatttgt gccgatatct aagcctattg
     5641 agtatttctt atccatagat cctttctcct ctttagatct tttgaattct tttctctatc
     5701 actgataggg agtggtaaaa taactctatc aacgatagag tgtcaacaaa aattaggaat
     5761 taatgatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
     5821 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
     5881 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
     5941 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
     6001 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
     6061 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
     6121 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
     6181 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
     6241 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
     6301 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
     6361 aacaacttaa atgtgaaagt gggtcttaag tgacggaaga tcacttcgca gaataaataa
     6421 atcctggtgt ccctgttgat accgggaagc cccgggccaa cttttggcga aaatgagacg
     6481 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     6541 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     6601 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     6661 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     6721 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     6781 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     6841 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     6901 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     6961 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     7021 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     7081 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     7141 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     7201 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     7261 attggtgccc gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
     7321 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag cactagtcat
     7381 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacgcag cgaactgaat
     7441 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg
     7501 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
     7561 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagt
//
