LOCUS       sequence-475992-       11584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one plant virus  vector to detect circular genomes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11584)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 11584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTc
FEATURES             Location/Qualifiers
     source          1..11584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4670..4687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4670..4686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4679..4701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4889..4913
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6214..6843
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7272..8345
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8411..8605
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8841..8863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8949..9089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9004..9023
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9104..9121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9275..9863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9355..9374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9950..10744)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11169..11193
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    11461..11482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11497..11502,11503..11520,11521..11527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11535..11551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11540..11562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11559..11575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11559..11575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtcat tcaagcacgt ggaaactcac
      961 gatcttcatg caactgcgac tgagtctagc tcacgttgac ggaaactgca gaacgatctc
     1021 agttaggtca tgggaaagct gatattcatc cctctgagac tctatataat taaatgcgtt
     1081 tgggggatta gctaattgag aatccatata gagaaatatg gccgcgcagc gacgccggtt
     1141 gaagaatatg aacaaggaat aagatgaaca aggaagaaga tgaacaagca ataatttaat
     1201 taattattgt taataagcat aaattatgaa aataattttg atggtaatga aagtattata
     1261 aataatattt aggagaaaat attgttaagg aaatatctgg ttatgtttat ttgttaaggt
     1321 atatgggttt ttatagaaac ccaggagcag ctatggtaag aaacccagga ctcaaaaaat
     1381 gcctctcaac ttctctcata tgttgcggag tctggagtcc catttatacc atagaaagca
     1441 ttgggggtat agcatggtaa tttcacatcg gactccatac cgcggccatc tatataatat
     1501 tactgatggc cgcgcgaagt cccggtatgc cttttcagcc gttggatctg gagccgtacc
     1561 tttttcgcgt tcccattttg cccctggagt cccgagctat ttacgacccc ctgccaccga
     1621 gtccccttcg tggagcgctc ttcgtccaac acgtgtccca acttttgcgc atttttgact
     1681 acctgtcgcg cacttgaatt aaagttgccc ttttattttc ttccaattat tttgcggctg
     1741 agtagtctag tggatccccc ggagctccac ttgggatagg ttaagaaaat atgtctggct
     1801 tgtaaacgaa aagaatcggg gtttctaggc attttttaaa tagtgaccca ggactcaaaa
     1861 actagctctc aacttctctc atatgttgtg gagtctggag tcctttatat atgtttacgc
     1921 tatagaaggc atttggggac tctcccgggc ccaccatagg actccatacc gcggccatct
     1981 atataatatt actgatggcc gcgcggattt tggagccccc ttttgtcgtt agcgcgacct
     2041 ttaattcaaa ttaaaggttg cttttttcta tcgtccgatc attttgcgcc tgactagcct
     2101 agatattagc aacaacttcg cggctaagtt gttgaccgtc aagtataaat taatgggctt
     2161 atggcctaat gggtatagag caaaatggca tgcctgcaga ctagtgcttt actctgatcc
     2221 gcagatattt catcaggaaa gagctttgca agttccttca ttgtagcatc aataatttct
     2281 gagtcactac atgcaatcca ttcttctgct ggggcaaaaa ctaactccaa catggattgg
     2341 tttggattgt aatattcctt acatgttaca gacatgtcag cataaacact taaaagcggg
     2401 cttctgctaa agagtagatg atcataggtg ttcttcaatt tcctatcaaa ccagatgtga
     2461 acgttgataa ctggaactcc aactaatttc tctaatttct tgaagtatga aatctctctc
     2521 cagtcttcag gcactagtga attcgctagc gttaactggc cataatcctg tgtatgcaac
     2581 gttgaaaatt cggatctatt tttacgattc gatcatgaat taataaaatt taaattttat
     2641 tgaatgattt tctagtacat aactgacata cgacttatca gttgcgaaac gaacagctct
     2701 aattacattg ttaagtgaaa ttacgcctaa ttcgtctaag tacaacataa ccaactgttt
     2761 aaacctaatt aaataagtcg acccagaagc tgtcagagat gtcgtccaga cttggaagtt
     2821 caggtaagct ttgtggagat gcaacgctct ccgtaagttg tggttgaatc ggatctggac
     2881 gtggtatata cgcgtcctgg tgtatagcag atcctctact ccgtatatct tgaaatatag
     2941 gggatttatt atctcccaga tatacacgcc attctctgcc tgacgtgcag tgatgatttc
     3001 ccctgtgcgt gaatccatga ccggtgcagc ctatgtggaa gtatatggtg cacccacagt
     3061 ttagatcaat gcgtcgtcgt ctaatcgccc tcctcttggc ttgcctgtgt gccttcttga
     3121 tagagggggg cgtcgagagt gatgaagata acattcttga gagtccaatg tttgagcgac
     3181 gcattttccg ctttatccag gaaatcttta tagctggatc cctcgcctgg attgcaaagc
     3241 acgattgaag ggatccctcc tttaatttga actggtttcc cgtacttgca atttgactgc
     3301 cagtcttttt gggccccaag aagctctttc caatgcttta gctttaggta ttgcggtgct
     3361 acgtcatcaa tgacgttata ggcaacatta ttcgagtaca ctcgattatt gaagtccagg
     3421 tgtccactga ggtaattatg tgggcctaaa gcacgagccc acatcgtctt gcccgttcta
     3481 gaatcacctt ccactatgag actaactggt cttacaggcc gcgcagcggg acccattcca
     3541 aaataatcat ccgcccattc ttgcatctgt tcaggaacgt tagtgaaaga ggaaagtgga
     3601 aatggaggga cccattgttc aggcgccttt ttgaaaaggc gttccaagtt cgctgtaatg
     3661 ttatggtaac tcacgatgaa cgcccttgga tctccggccc ggataattgc aagagcttcc
     3721 gccaaactag gtgcattgat ggcgttataa tagacgtcgt ctttattctt ctttgtaccc
     3781 ccaggcactt tgaactgtcc ggattcacaa caatcgccgt ctttggtgat gtaattcttg
     3841 acggcgttgg cgtctttggc tgcttgtatg tttgggtgaa aattggtcga ccttctgggg
     3901 tgagtaaggt cgaaaaatct cttattcttg atgttggact ttccagagag ttggatgaga
     3961 cagtgaaggt gtgggaatcc gtccgagtgc tcctctctgg caactcgaat atatgttggt
     4021 ttgacgactg accatgataa ggtttgaagc atttgaagag cttcattttt tgggatatcg
     4081 cacttgggat aggttaagaa aatatgtctg gcttgtaaac gaaaagaatc ggggtttcta
     4141 ggcatttttt aaatagtgac ccaggactca aaaactagct ctcaacttct ctcatatgtt
     4201 gtggagtctg gagtccttta tatatgttta cgctatagaa ggcatttggg gactctcccg
     4261 ggcccaccat aggactccat accgcggcca tctatataat attactgatg gccgcgcgga
     4321 ttttggagcc cccttttgtc gttagcgcga cctttaattc aaattaaagg ttgctttttt
     4381 ctatcgtcca atcattttgc gcctgactag cctagatatt agcaacaact tcgcgactaa
     4441 gttgttgacc gtcaagtata aattaatggg cttatggccc aatgggtata gagcaaaatg
     4501 cccaagcgtg atgccccgtg gcgcttatta gcgggtacgt ctaaggtaag tcgaaatgcc
     4561 aactactctc ccttgagagt attgggccct aaagttaata agggacctcg agggggggcc
     4621 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg
     4681 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4741 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4801 agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt
     4861 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     4921 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     4981 gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc
     5041 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
     5101 acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc
     5161 gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac
     5221 attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg
     5281 acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt
     5341 tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc
     5401 acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc
     5461 gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg
     5521 cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg
     5581 gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg
     5641 ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc
     5701 acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg
     5761 gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg
     5821 aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg
     5881 ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga
     5941 accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga
     6001 gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc
     6061 caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa
     6121 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     6181 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     6241 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     6301 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     6361 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     6421 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     6481 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     6541 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     6601 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     6661 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     6721 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     6781 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     6841 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     6901 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     6961 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     7021 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     7081 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     7141 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     7201 ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc
     7261 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     7321 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     7381 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     7441 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     7501 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     7561 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     7621 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     7681 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     7741 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     7801 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     7861 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     7921 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     7981 ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca
     8041 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     8101 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     8161 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     8221 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     8281 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     8341 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     8401 gtgtctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     8461 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     8521 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     8581 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     8641 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     8701 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac
     8761 aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt
     8821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     8881 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     8941 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     9001 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9061 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     9121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9901 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     9961 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
    10021 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
    10081 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
    10141 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
    10201 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
    10261 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
    10321 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
    10381 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
    10441 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    10501 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    10561 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    10621 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    10681 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    10741 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    10801 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    10861 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    10921 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
    10981 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
    11041 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
    11101 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
    11161 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
    11221 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg atagcccttt
    11281 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
    11341 tgttggcaag ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11401 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11461 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11521 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    11581 tacg
//
