LOCUS       sequence-475995-       13300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhSP gene
            via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13300)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS-GhSP
FEATURES             Location/Qualifiers
     source          1..13300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6386..6403)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6386..6402)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6395..6417)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6605..6629
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7930..8559
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8988..10061
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10127..10321
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10557..10579)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10665..10805
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10720..10739
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10820..10837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10991..11579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11071..11090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11666..12460)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12885..12909
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13177..13198
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13213..13218,13219..13236,13237..13243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13251..13267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13256..13278
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13275..13291
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13275..13291
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgc gtcttctagc
     3781 agctgtttcc ctttgagcat tgaaatagac agctgcaaca ggaaccccta gttcgttttc
     3841 ttctgcaaac ttcctggtat cgaaacgatc ccttgatgac ggtatgcttc tcactgtttg
     3901 cctgcctttt tgcttgaaga ggaggaacac aaacctgtgg atccctatgt ttggccttgg
     3961 catttcgtag ttcaccattt cccttccaaa tgtggcatct gttgtgccgg ggatatctgt
     4021 cactatccag tgtaagtgct ccctcaggta agggtcacta ggaccaggaa catctgggtc
     4081 tgtcatcacc agggtgaaaa aggatctcat atcacctcca tgaacctcaa ccttaggctt
     4141 gttagtaact gcagatggaa aaaattcatg gccattatat acctgcttgt tggtgttgaa
     4201 agtgactgac attttcacag atggggagag ggcatcaata acatccccaa tcactcattc
     4261 gctagcgtta actggccata atcctgtgta tgcaacgttg aaaattcgga tctattttta
     4321 cgattcgatc atgaattaat aaaatttaaa ttttattgaa tgattttcta gtacataact
     4381 gacatacgac ttatcagttg cgaaacgaac agctctaatt acattgttaa gtgaaattac
     4441 gcctaattcg tctaagtaca acataaccaa ctgtttaaac ctaattaaat aagtcgaccc
     4501 agaagctgtc agagatgtcg tccagacttg gaagttcagg taagctttgt ggagatgcaa
     4561 cgctctccgt aagttgtggt tgaatcggat ctggacgtgg tatatacgcg tcctggtgta
     4621 tagcagatcc tctactccgt atatcttgaa atatagggga tttattatct cccagatata
     4681 cacgccattc tctgcctgac gtgcagtgat gatttcccct gtgcgtgaat ccatgaccgg
     4741 tgcagcctat gtggaagtat atggtgcacc cacagtttag atcaatgcgt cgtcgtctaa
     4801 tcgccctcct cttggcttgc ctgtgtgcct tcttgataga ggggggcgtc gagagtgatg
     4861 aagataacat tcttgagagt ccaatgtttg agcgacgcat tttccgcttt atccaggaaa
     4921 tctttatagc tggatccctc gcctggattg caaagcacga ttgaagggat ccctccttta
     4981 atttgaactg gtttcccgta cttgcaattt gactgccagt ctttttgggc cccaagaagc
     5041 tctttccaat gctttagctt taggtattgc ggtgctacgt catcaatgac gttataggca
     5101 acattattcg agtacactcg attattgaag tccaggtgtc cactgaggta attatgtggg
     5161 cctaaagcac gagcccacat cgtcttgccc gttctagaat caccttccac tatgagacta
     5221 actggtctta caggccgcgc agcgggaccc attccaaaat aatcatccgc ccattcttgc
     5281 atctgttcag gaacgttagt gaaagaggaa agtggaaatg gagggaccca ttgttcaggc
     5341 gcctttttga aaaggcgttc caagttcgct gtaatgttat ggtaactcac gatgaacgcc
     5401 cttggatctc cggcccggat aattgcaaga gcttccgcca aactaggtgc attgatggcg
     5461 ttataataga cgtcgtcttt attcttcttt gtacccccag gcactttgaa ctgtccggat
     5521 tcacaacaat cgccgtcttt ggtgatgtaa ttcttgacgg cgttggcgtc tttggctgct
     5581 tgtatgtttg ggtgaaaatt ggtcgacctt ctggggtgag taaggtcgaa aaatctctta
     5641 ttcttgatgt tggactttcc agagagttgg atgagacagt gaaggtgtgg gaatccgtcc
     5701 gagtgctcct ctctggcaac tcgaatatat gttggtttga cgactgacca tgataaggtt
     5761 tgaagcattt gaagagcttc attttttggg atatcgcact tgggataggt taagaaaata
     5821 tgtctggctt gtaaacgaaa agaatcgggg tttctaggca ttttttaaat agtgacccag
     5881 gactcaaaaa ctagctctca acttctctca tatgttgtgg agtctggagt cctttatata
     5941 tgtttacgct atagaaggca tttggggact ctcccgggcc caccatagga ctccataccg
     6001 cggccatcta tataatatta ctgatggccg cgcggatttt ggagccccct tttgtcgtta
     6061 gcgcgacctt taattcaaat taaaggttgc ttttttctat cgtccaatca ttttgcgcct
     6121 gactagccta gatattagca acaacttcgc gactaagttg ttgaccgtca agtataaatt
     6181 aatgggctta tggcccaatg ggtatagagc aaaatgccca agcgtgatgc cccgtggcgc
     6241 ttattagcgg gtacgtctaa ggtaagtcga aatgccaact actctccctt gagagtattg
     6301 ggccctaaag ttaataaggg acctcgaggg ggggcccggt acccggggat cctctagagt
     6361 cgacctgcag gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     6421 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     6481 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     6541 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
     6601 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     6661 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     6721 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
     6781 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
     6841 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
     6901 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
     6961 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
     7021 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
     7081 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
     7141 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
     7201 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
     7261 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
     7321 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
     7381 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
     7441 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
     7501 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
     7561 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
     7621 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
     7681 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt
     7741 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
     7801 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
     7861 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
     7921 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
     7981 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
     8041 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
     8101 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
     8161 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
     8221 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
     8281 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
     8341 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
     8401 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
     8461 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
     8521 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
     8581 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
     8641 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
     8701 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
     8761 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
     8821 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
     8881 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
     8941 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
     9001 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
     9061 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
     9121 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
     9181 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
     9241 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
     9301 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
     9361 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
     9421 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
     9481 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
     9541 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
     9601 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
     9661 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
     9721 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
     9781 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
     9841 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
     9901 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
     9961 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    10021 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    10081 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtgt ctttcctgtg gatagcacgt
    10141 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    10201 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    10261 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    10321 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    10381 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    10441 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    10501 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    10561 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    10621 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    10681 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    10741 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    10801 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11401 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    11461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11641 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
    11701 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    11761 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    11821 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    11881 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    11941 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    12001 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    12061 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    12121 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    12181 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    12241 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    12301 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    12361 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    12421 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    12481 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    12541 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    12601 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
    12661 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    12721 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    12781 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    12841 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt
    12901 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac
    12961 tgaattaacg ccgaattaat tcggggatag ccctttggtc ttctgagact gtatctttga
    13021 tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat
    13081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    13141 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    13201 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    13261 ataacaattt cacacaggaa acagctatga catgattacg
//
