LOCUS       sequence-475996-       13340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhCHLI 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13340)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS-GhCHLI
FEATURES             Location/Qualifiers
     source          1..13340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6426..6443)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6426..6442)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6435..6457)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6645..6669
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7970..8599
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9028..10101
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10167..10361
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10597..10619)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10705..10845
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10760..10779
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10860..10877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11031..11619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11111..11130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11706..12500)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12925..12949
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    13217..13238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13253..13258,13259..13276,13277..13283)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13291..13307
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13296..13318
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13315..13331
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13315..13331
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtgt tggggatgac
     3781 agtggcaata tcttctgcaa tgactttatc tcttccttta agagctgcaa gagcttttgc
     3841 agctctatta gtgacaatat ctcctctcaa tccatcaaca ttcaactcag cgcaaacctt
     3901 tgatatttta acctttaggt cttgatcaat ctgaacagaa gaaagagaac tcctagctga
     3961 agcaatctgc tgttggagct tctcttgctc tgccttgtaa gaatcacgga attcttttgg
     4021 gtttttatca aaccgtgctc tttcctccac aatcttcact ctaagctcag cgtccctcac
     4081 tgtcccgact tgagcatgca ttccgaatcg atcaagaagc tgtggtctaa gctctccttc
     4141 ttctggatta cctgagccga tgagaataaa ccgagcagga tgtgagatcg aaataccttc
     4201 cctctcaaca gtgttccatc ctgatgcagc ggaatccaga agaacatcca ccaaatggtc
     4261 atctaaaagg ttaacttcat cgacgtaaac tagtgaattc gctagcgtta actggccata
     4321 atcctgtgta tgcaacgttg aaaattcgga tctattttta cgattcgatc atgaattaat
     4381 aaaatttaaa ttttattgaa tgattttcta gtacataact gacatacgac ttatcagttg
     4441 cgaaacgaac agctctaatt acattgttaa gtgaaattac gcctaattcg tctaagtaca
     4501 acataaccaa ctgtttaaac ctaattaaat aagtcgaccc agaagctgtc agagatgtcg
     4561 tccagacttg gaagttcagg taagctttgt ggagatgcaa cgctctccgt aagttgtggt
     4621 tgaatcggat ctggacgtgg tatatacgcg tcctggtgta tagcagatcc tctactccgt
     4681 atatcttgaa atatagggga tttattatct cccagatata cacgccattc tctgcctgac
     4741 gtgcagtgat gatttcccct gtgcgtgaat ccatgaccgg tgcagcctat gtggaagtat
     4801 atggtgcacc cacagtttag atcaatgcgt cgtcgtctaa tcgccctcct cttggcttgc
     4861 ctgtgtgcct tcttgataga ggggggcgtc gagagtgatg aagataacat tcttgagagt
     4921 ccaatgtttg agcgacgcat tttccgcttt atccaggaaa tctttatagc tggatccctc
     4981 gcctggattg caaagcacga ttgaagggat ccctccttta atttgaactg gtttcccgta
     5041 cttgcaattt gactgccagt ctttttgggc cccaagaagc tctttccaat gctttagctt
     5101 taggtattgc ggtgctacgt catcaatgac gttataggca acattattcg agtacactcg
     5161 attattgaag tccaggtgtc cactgaggta attatgtggg cctaaagcac gagcccacat
     5221 cgtcttgccc gttctagaat caccttccac tatgagacta actggtctta caggccgcgc
     5281 agcgggaccc attccaaaat aatcatccgc ccattcttgc atctgttcag gaacgttagt
     5341 gaaagaggaa agtggaaatg gagggaccca ttgttcaggc gcctttttga aaaggcgttc
     5401 caagttcgct gtaatgttat ggtaactcac gatgaacgcc cttggatctc cggcccggat
     5461 aattgcaaga gcttccgcca aactaggtgc attgatggcg ttataataga cgtcgtcttt
     5521 attcttcttt gtacccccag gcactttgaa ctgtccggat tcacaacaat cgccgtcttt
     5581 ggtgatgtaa ttcttgacgg cgttggcgtc tttggctgct tgtatgtttg ggtgaaaatt
     5641 ggtcgacctt ctggggtgag taaggtcgaa aaatctctta ttcttgatgt tggactttcc
     5701 agagagttgg atgagacagt gaaggtgtgg gaatccgtcc gagtgctcct ctctggcaac
     5761 tcgaatatat gttggtttga cgactgacca tgataaggtt tgaagcattt gaagagcttc
     5821 attttttggg atatcgcact tgggataggt taagaaaata tgtctggctt gtaaacgaaa
     5881 agaatcgggg tttctaggca ttttttaaat agtgacccag gactcaaaaa ctagctctca
     5941 acttctctca tatgttgtgg agtctggagt cctttatata tgtttacgct atagaaggca
     6001 tttggggact ctcccgggcc caccatagga ctccataccg cggccatcta tataatatta
     6061 ctgatggccg cgcggatttt ggagccccct tttgtcgtta gcgcgacctt taattcaaat
     6121 taaaggttgc ttttttctat cgtccaatca ttttgcgcct gactagccta gatattagca
     6181 acaacttcgc gactaagttg ttgaccgtca agtataaatt aatgggctta tggcccaatg
     6241 ggtatagagc aaaatgccca agcgtgatgc cccgtggcgc ttattagcgg gtacgtctaa
     6301 ggtaagtcga aatgccaact actctccctt gagagtattg ggccctaaag ttaataaggg
     6361 acctcgaggg ggggcccggt acccggggat cctctagagt cgacctgcag gcatgcaagc
     6421 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6541 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
     6601 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     6661 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     6721 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     6781 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     6841 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     6901 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     6961 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     7021 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     7081 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     7141 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     7201 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     7261 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     7321 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     7381 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     7441 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     7501 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     7561 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     7621 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     7681 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     7741 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     7801 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     7861 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     7921 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     7981 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     8041 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     8101 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     8161 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     8221 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     8281 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     8341 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     8401 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     8461 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     8521 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     8581 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     8641 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     8701 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     8761 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     8821 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     8881 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     8941 aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac
     9001 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     9061 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     9121 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     9181 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     9241 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     9301 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     9361 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     9421 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     9481 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     9541 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     9601 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     9661 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     9721 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     9781 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     9841 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     9901 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     9961 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
    10021 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
    10081 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
    10141 gaaaaaggtc gaaaaggtgt ctttcctgtg gatagcacgt acattgggaa cccaaagccg
    10201 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
    10261 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
    10321 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
    10381 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
    10441 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
    10501 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
    10561 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10621 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10681 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
    10741 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
    10861 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10921 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10981 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    11041 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    11101 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    11161 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11221 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11281 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    11341 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    11401 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    11461 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11521 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11581 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11641 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
    11701 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
    11761 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    11821 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    11881 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    11941 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    12001 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    12061 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    12121 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    12181 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
    12241 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
    12301 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    12361 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    12421 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    12481 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
    12541 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    12601 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    12661 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
    12721 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
    12781 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
    12841 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    12901 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    12961 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
    13021 tcggggatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    13081 gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg
    13141 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
    13201 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    13261 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    13321 acagctatga catgattacg
//
