LOCUS       sequence-475998-       13112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one vector is used to specifically silence the GhOMT1 
            gene via virus-induced gene silencing (VIGS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVS-GhOMT1_2
FEATURES             Location/Qualifiers
     source          1..13112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6198..6215)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6198..6214)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6207..6229)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6417..6441
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7742..8371
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8800..9873
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9939..10133
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10369..10391)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10477..10617
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10532..10551
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10632..10649)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10803..11391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10883..10902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11478..12272)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12697..12721
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    12989..13010
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13025..13030,13031..13048,13049..13055)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13063..13079
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13068..13090
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13087..13103
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13087..13103
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagttg atgcccttga
     3781 tttgaggata ctttgaagta ataaaactga gagcaacccc aatcccacca cccacatcaa
     3841 ccaacacttt caacccatca aaccctttgt aaacatcaac aatcttcctc attatcaaag
     3901 cagtatgatt tgacattgcc tggttaaata ctctattgaa tcgttgatca gttcctggat
     3961 attcaaatgc tgtcatcccg tatgccttac taaaaggaac ccctccttct agtatagcat
     4021 catttaaatg gtaccagctt tgcatgaaga cttggtcatg gtgcaacaat tcgctagcgt
     4081 taactggcca taatcctgtg tatgcaacgt tgaaaattcg gatctatttt tacgattcga
     4141 tcatgaatta ataaaattta aattttattg aatgattttc tagtacataa ctgacatacg
     4201 acttatcagt tgcgaaacga acagctctaa ttacattgtt aagtgaaatt acgcctaatt
     4261 cgtctaagta caacataacc aactgtttaa acctaattaa ataagtcgac ccagaagctg
     4321 tcagagatgt cgtccagact tggaagttca ggtaagcttt gtggagatgc aacgctctcc
     4381 gtaagttgtg gttgaatcgg atctggacgt ggtatatacg cgtcctggtg tatagcagat
     4441 cctctactcc gtatatcttg aaatataggg gatttattat ctcccagata tacacgccat
     4501 tctctgcctg acgtgcagtg atgatttccc ctgtgcgtga atccatgacc ggtgcagcct
     4561 atgtggaagt atatggtgca cccacagttt agatcaatgc gtcgtcgtct aatcgccctc
     4621 ctcttggctt gcctgtgtgc cttcttgata gaggggggcg tcgagagtga tgaagataac
     4681 attcttgaga gtccaatgtt tgagcgacgc attttccgct ttatccagga aatctttata
     4741 gctggatccc tcgcctggat tgcaaagcac gattgaaggg atccctcctt taatttgaac
     4801 tggtttcccg tacttgcaat ttgactgcca gtctttttgg gccccaagaa gctctttcca
     4861 atgctttagc tttaggtatt gcggtgctac gtcatcaatg acgttatagg caacattatt
     4921 cgagtacact cgattattga agtccaggtg tccactgagg taattatgtg ggcctaaagc
     4981 acgagcccac atcgtcttgc ccgttctaga atcaccttcc actatgagac taactggtct
     5041 tacaggccgc gcagcgggac ccattccaaa ataatcatcc gcccattctt gcatctgttc
     5101 aggaacgtta gtgaaagagg aaagtggaaa tggagggacc cattgttcag gcgccttttt
     5161 gaaaaggcgt tccaagttcg ctgtaatgtt atggtaactc acgatgaacg cccttggatc
     5221 tccggcccgg ataattgcaa gagcttccgc caaactaggt gcattgatgg cgttataata
     5281 gacgtcgtct ttattcttct ttgtaccccc aggcactttg aactgtccgg attcacaaca
     5341 atcgccgtct ttggtgatgt aattcttgac ggcgttggcg tctttggctg cttgtatgtt
     5401 tgggtgaaaa ttggtcgacc ttctggggtg agtaaggtcg aaaaatctct tattcttgat
     5461 gttggacttt ccagagagtt ggatgagaca gtgaaggtgt gggaatccgt ccgagtgctc
     5521 ctctctggca actcgaatat atgttggttt gacgactgac catgataagg tttgaagcat
     5581 ttgaagagct tcattttttg ggatatcgca cttgggatag gttaagaaaa tatgtctggc
     5641 ttgtaaacga aaagaatcgg ggtttctagg cattttttaa atagtgaccc aggactcaaa
     5701 aactagctct caacttctct catatgttgt ggagtctgga gtcctttata tatgtttacg
     5761 ctatagaagg catttgggga ctctcccggg cccaccatag gactccatac cgcggccatc
     5821 tatataatat tactgatggc cgcgcggatt ttggagcccc cttttgtcgt tagcgcgacc
     5881 tttaattcaa attaaaggtt gcttttttct atcgtccaat cattttgcgc ctgactagcc
     5941 tagatattag caacaacttc gcgactaagt tgttgaccgt caagtataaa ttaatgggct
     6001 tatggcccaa tgggtataga gcaaaatgcc caagcgtgat gccccgtggc gcttattagc
     6061 gggtacgtct aaggtaagtc gaaatgccaa ctactctccc ttgagagtat tgggccctaa
     6121 agttaataag ggacctcgag ggggggcccg gtacccgggg atcctctaga gtcgacctgc
     6181 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6241 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6301 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
     6361 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     6421 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     6481 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     6541 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     6601 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     6661 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     6721 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     6781 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     6841 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     6901 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     6961 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     7021 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     7081 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     7141 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     7201 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     7261 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     7321 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     7381 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     7441 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     7501 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     7561 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     7621 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     7681 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     7741 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     7801 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     7861 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     7921 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     7981 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     8041 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     8101 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     8161 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     8221 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     8281 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     8341 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     8401 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     8461 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     8521 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     8581 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     8641 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     8701 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
     8761 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     8821 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     8881 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     8941 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     9001 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     9061 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     9121 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     9181 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     9241 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     9301 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     9361 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     9421 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     9481 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     9541 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     9601 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     9661 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     9721 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     9781 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     9841 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     9901 gccctagcag gggaaaaagg tcgaaaaggt gtctttcctg tggatagcac gtacattggg
     9961 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    10021 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    10081 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    10141 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    10201 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    10261 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    10321 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10381 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10441 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    10501 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    10561 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10621 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10681 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10741 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10801 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10861 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10921 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10981 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11041 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11101 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11161 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11221 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    11281 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11341 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11401 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11461 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    11521 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    11581 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    11641 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    11701 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    11761 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    11821 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    11881 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    11941 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    12001 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    12061 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    12121 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    12181 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    12241 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    12301 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    12361 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    12421 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    12481 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    12541 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    12601 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    12661 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    12721 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
    12781 cgccgaatta attcggggat agccctttgg tcttctgaga ctgtatcttt gatattcttg
    12841 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
    12901 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    12961 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    13021 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    13081 ttcacacagg aaacagctat gacatgatta cg
//
